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  • 1
    Electronic Resource
    Electronic Resource
    Effect of dehydroepiandrosterone sulfate on the mineral accretion of chick embryo frontal bones cultivatedin vitro (1969)
    Springer
    Calcified tissue international 4 (1969), S. 39-47 
    Details
    ISSN: 1432-0827
    Keywords: Dehydroepiandrosterone ; Calcification ; Embryo ; Tissue Culture ; Bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Les modes d'utilisation de glucose, le contenu de calcium et d'hydroxyproline et la densité cellulaire du perioste de les os frontaux d'embryons de poulet de 12 et 13 jours de developpement, cultivés sur coagulum de plasma, se presentant différenment à chaque âge. Cultivés avec sulfate de déhydroèpiandrostérone en concentration 1 mM, les frontaux de 12 jours montrent un synthese augmentée du matrice osseuse, celle de 13 jours se calcifient à une vélocité significativement plus grande que celle des os contôles. Le degré de calcification au quatrième jour de culture measuré par la relation calcium/hydroxyproline, suit un fonction lineáire avec le logarithme des doses de sulfate de dehydroepiandrostérone employées (0.5, 1,0 et 2,0 mM). Les renseignements obtenus indiquent que les frontaux de 13 jours, cultivés “in vitro” constituent modeles experimentaux appropriés pour étudier l'effet des androgénes sur le tissue osseux.
    Abstract: Zusammenfassung Stirnbeine von Hühnerembryonen an ihrem 12. und 13. Entwicklungstag entnommen und in vitro kultiviert zeigen verschiedene Arten der Glucoseverwertung der Periostzellendichte, des Calcium- und Hydroxyprolingehaltes. Wird Dehydroepiandrosteronsulfat dem Medium in einer 1 mM-Konzentration zugegeben, so beteiligen sich die 12tägigen Stirnbeine vorwiegend an der Knochengewebesynthese, während die 13tägigen signifikant stärker verkalken als die Kontrollen. Gemessen an der Calcium/hydroxyprolin Ratio bildet die Verkalkung der 13tägigen Stirnbeine eine lineare Funktion mit den Logarithmen der verwendeten Dosen von Dehydroepiandrosteronsulfat (0,5, 1,0 und 2,0 mM). Das in vitro kultivierte 13tägige Stirnbein schein ein geeignetes Experimentiermodell zur Studie der Dehydroepiandrosteronsulfatwirkung auf das Knochengewebe zu sein, weil es das grundlegende Phänomen (erhöhte Verkalkung) wiedergibt, welches man auch bei mit Androgenen behandelten Menschen und Tieren beobachtet.
    Notes: Abstract Chick embryo frontal bones at 12 and 13 days of development cultivatedin vitro exhibit different patterns of glucose utilization, periosteal cellular density and calcium and hydroxyproline content. When dehydroepiandrosterone sulfate is added to the medium at a concentration 1 mM, 12-day frontals engage primarily in osteoid tissue synthesis while 13-day frontals calcify at a significantly greater rate than controls. Measured with the ratio calcium/hydroxyproline, the calcification of 13-day frontals follows a linear function with the logarithm of the doses of dehydroepiandrosterone sulfate employed (0.5, 1.0 and 2.0 mM). The 13-day frontal bone cultivatedin vitro seems to be an adequate experimental model for the study of the effects of dehydroepiandrosterone sulfate on bone tissue because it reproduces the basic phenomenon (increased calcification) observed in man and animals treated with androgens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02279104
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  • 2
    Electronic Resource
    Electronic Resource
    Controlled clinical trial of cadralazine as a second-step drug in the treatment of hypertension (1985)
    Springer
    European journal of clinical pharmacology 28 (1985), S. 135-138 
    Details
    ISSN: 1432-1041
    Keywords: hypertension ; cadralazine ; vasodilators ; chlorthalidone ; atenolol ; side-effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The antihypertensive efficacy of a new long-lasting vasodilator, cadralazine, and the diuretic chlorthalidone have been compared in hypertensive patients receiving concurrent treatment with atenolol. After a 4-week run-in period with atenolol alone 100 mg/day, two groups of 10 patients whose diastolic blood pressure exceeded 100 mm Hg were given for a period of 65 days either cadralazine 15 mg/day or chlorthalidone 25 mg/day, according to a randomized, double-blind, between-patients design. Compared to atenolol alone, both cadralazine and chlorthalidone induced a statistically and clinically significant decrease in blood pressure. The antihypertensive effect did not differ significantly between groups. Good compensation of the atenolol-induced decrease in heart rate was obtained with cadralazine, whereas during atenolol + chlorthalidone treatment at times the standing heart rate was significantly lower than during treatment with atenolol + cadralazine. Side-effects, many of which were already present during atenolol treatment, occurred with a similar frequency in both groups. It is concluded that atenolol + cadralazine and atenolol + chlorthalidone are equally well tolerated, acceptable and effective in the treatment of hypertension, but that further studies are warranted to explore the potential haemodynamic advantages of the cadralazine + atenolol combination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00609680
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  • 3
    Electronic Resource
    Electronic Resource
    Post-treatment diagnostic accuracy of a new enzyme immunoassay to detect Helicobacter pylori in stools (2001)
    Oxford UK : Blackwell Science Ltd
    Alimentary pharmacology & therapeutics 15 (2001), S. 0 
    Details
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Helicobacter pylori has attracted increasing attention among gastroenterologists because of its pathogenic potential, stimulating the search for non-invasive diagnostic tests.〈section xml:id="abs1-2"〉〈title type="main"〉Aims:In this study the efficacy of a new enzyme immunoassay designed to detect H. pylori antigens in stools (HpSA) was evaluated before and after eradication therapy.〈section xml:id="abs1-3"〉〈title type="main"〉Methods:HpSA was performed on stool samples collected from 268 patients whose H. pylori status was defined on the basis of concordant results for the 13C-urea breath test, rapid urease test and histology. The H. pylori-positive patients were treated with a 1-week triple therapy to eradicate the infection. One (T30) and 3 months (T90) after the end of therapy, 13C-urea breath test and HpSA were repeated in the treated patients.〈section xml:id="abs1-4"〉〈title type="main"〉Results:The overall diagnostic accuracy of HpSA at T30 (83%, 95% confidence interval (CI) 77–89%) was significantly lower in comparison to the values obtained at baseline (94%, 95% CI: 91–97%) and at T90 (97%, 95% CI: 94–99%). No significant difference was found between the diagnostic accuracy of HpSA at baseline and at T90 (P=0.253).〈section xml:id="abs1-5"〉〈title type="main"〉Conclusions:The present data suggest that HpSA provides a low diagnostic accuracy when used shortly after treatment. It needs a longer period of follow-up (8–12 weeks) to reach a reliability comparable to the 13C-urea breath test.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2036.2001.00928.x
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  • 4
    Electronic Resource
    Electronic Resource
    Gene Expression in Human Macrophages Infected with Dengue Virus Serotype-2 (2004)
    Oxford, UK; Malden, USA : Blackwell Science Ltd
    Scandinavian journal of immunology 60 (2004), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Infection by any of the four serotypes of dengue viruses (DEN-1, -2, -3 and -4) may result in either a relatively benign fever, called dengue fever (DF), a fatal disease, such as dengue haemorrhagic fever (DHF) or dengue shock syndrome (DSS). Several lines of evidence suggest that soluble immune response mediators may be involved in the severity of dengue infections. For instance, elevated seric levels of IL-8 are a common feature in DHF patients. Because other chemokines, cytokines, adhesion molecules, chemokine and cytokine receptors, as well as cytokine-related molecules may also be involved in dengue virus pathogenesis, we aimed at analysing the gene expression of such molecules in the course of an in vitro DEN-2 infection of human peripheral blood monocyte-derived macrophages, a cell type regarded as a primary target for DEN. Nylon membrane gene arrays containing 375 different human cytokine-related genes were used as a first step to search for differentially expressed genes upon infection. Transcripts for IL-8, IL-1β, osteopontin, GRO-α, -β and -γ, I-309, and some other molecules showed to be upregulated upon infection, whereas others such as MIC-1, CD27L and CD30L, were downregulated. Four genes were selected for reverse transcriptase-polymerase chain reaction based gene-expression analysis as a way to partially confirm microarray results. This approach pointed out 25 macrophage-expressed cytokine-related genes that could be relevant in DEN-2 pathogenesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.0300-9475.2004.01519.x
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  • 5
    Electronic Resource
    Electronic Resource
    Postnatal Development of Ley dig Cells in the Testis of the Domestic Fowl (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 513 (1987), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1987.tb25034.x
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  • 6
    Electronic Resource
    Electronic Resource
    Characterization of a partial deletion of the factor VIII gene in a haemophiliac with inhibitor (1988)
    Springer
    Human genetics 〈Berlin〉 79 (1988), S. 86-88 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Genomic DNA from 49 Italian patients affected with severe haemophilia A was analysed by Southern blotting technique using a cDNA probe corresponding to exons 14–26 of coagulation factor VIII. No TaqI site mutation was observed in this sample. A partial deletion, eliminating exons 15–18 and spanning about 13 kb, was identified and characterized in one patient with anti-factor VIII antibodies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00291718
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  • 7
    Electronic Resource
    Electronic Resource
    Isolation procedure for bovine retinal rod outer segments (1980)
    Springer
    Cellular and molecular life sciences 36 (1980), S. 894-895 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An isolation procedure to obtain rod outer segments from cattle retinas is reported. Centrifugation of homogenates in discontinuous and continuous sucrose density gradients yields purified photoreceptor cell outer segments. Assay of the final preparation for rhodopsin content gives a ratio of 2.4 for DO280 nm/DO498 nm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01953777
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  • 8
    Electronic Resource
    Electronic Resource
    Polymorphism in the location of the 18S and 28S rRNA genes on the chromosomes of the diploid-tetraploid treefrogs Hyla chrysoscelis and H. versicolor (1989)
    Springer
    Chromosoma 97 (1989), S. 481-487 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The chromosomes of the diploid Hyla chrysoscelis and its tetraploid sibling species H. versicolor were studied with AgNO3 staining and in situ hybridization to determine the chromosome location of the 18S and 28S ribosomal RNA (rRNA) genes. A total of 236 Hyla chrysoscelis from 34 localities in 15 U.S. states and 100 H. versicolor from 15 localities in 12 states were examined. The rRNA gene sites were extremely variable in H. chrysoscelis, and also variable, but to a lesser extent, in H. versicolor. The most common rRNA gene site in both H. chrysoscelis and H. versicolor was on the short arm of chromosome 6. All of the rRNA gene locations seen in H. versicolor were also seen in H. chrysoscelis, supporting the hypothesis that the tetraploid H. versicolor arose from H. chrysoscelis. Although polymorphic rRNA gene sites in H. versicolor may reflect the positions of the rRNA genes in H. chrysoscelis ancestors, the origin of the extreme variability of such sites in H. chrysoscelis seems more obscure. Possible explanations include inversions, translocations, mobile genetic elements or a combination of some or all of these.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00295033
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  • 9
    Electronic Resource
    Electronic Resource
    The effect of dehydroepiandrosterone sulfate and testosterone on the development of chick embryo frontal bones in vitro (1968)
    Springer
    Calcified tissue international 2 (1968), S. 133-144 
    Details
    ISSN: 1432-0827
    Keywords: Dehydroepiandrosterone ; Testosterone ; Embryo ; Tissue culture ; Frontal bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Les os frontaux d'embryons de poulet, de 12 jours de développement, cultivés sur coagulum de plasma avec sulfate de déhydroépiandrostérone (DS) en concentration 1 mM, montrent hyperplasie du périoste et synthèse augmentée du matrice osseuse. Ces phénomenes ne son pas vus quand on emploie les os frontaux de 11 jours de développement. La testostérone produit les mêmes effets que le DS. Le deux steroïdes semblent agir directement sur l'os selon on peut le déduire des conditions expérimentales. L'activité de la phosphatase alcaline des frontaux d'embryons de poulet de 12 jours de développement est augmentée de manière significative quand les rudiments son cultivés dans un milieu qui contient DS ou testostérone. Dans ces deux cas: a) on peut révéler l'activation de l'enzyme par des expériences de cinétique enzymatique et b) les vélocités maximes apparantes ont une grande correlation avec le logarithme des doses employées. Quand les deux steroïdes son essayés en conditions semblables, les deux produisent la même augmentation de l'activité phosphatasique.
    Abstract: Zusammenfassung Wenn Stirnknochen von 12 Tage alten Hühnerembryonen in vitro auf koaguliertem Plasma mit Zusatz von 1 mM Dehydroepiandrosteronsulfat gezüchtet werden, so zeigen sie eine Hyperplasie des Periostes und eine erhöhte Synthese des Osteoidgewebes. Diese Erscheinung trifft nicht zu, ween 11 tägige Stirnknochen in gleicher Weise gezüchtet werden. Mit Testosteron werden gleiche Effekte wie mit Dehydroepiandrosteronsulfat erzielt. Aus den gewählten Versuchsverhältnissen kann abgeleitet werden, daß beide Steroide direkt auf den Knochen zu wirken scheinen. Die Aktivität der alkalischen Phosphatase im Stirnknochen des 12 Tage alten Hühnerembryos ist signifikant erhöht, wenn die Knochenansätze in einem Dehydroepiandrosteron oder Testosteron enthaltenden Medium gezüchtet werden. In beiden Fällen ergeben enzymkinetische Untersuchungen einerseits eine Aktivierung des Enzyms, andererseits eine gute Korrelation der erhaltenen scheinbaren Maximalgeschwindigkeiten mit dem Logarithmus der verwendeten Dosen. Bei gleichzeitiger Prüfung bewirkten beide Steroide gleichzeitig eine ähnliche Aktivitätszunahme der alkalischen Phosphatase.
    Notes: Abstract Chick embryo frontal bones at 12 days of development, cultivated in vitro on plasma clots with dehydroepiandrosterone sulfate in a concentration 1mM, exhibit periosteal hyperplasia and increased synthesis of osteoid tissue. These phenomena are not observed when 11-day frontals are cultivated in similar conditions. Testosterone produces the same effects as dehydroepiandrosterone sulfate. Both steroids seem to act directly on bone as can be inferred from the experimental conditions employed. The alkaline phosphatase activity of chick embryo frontal bones at 12 days of development is significantly increased when the rudiments are cultivated in a medium containing dehydro-epiandrosterone or testosterone. In both cases a) enzyme kinetics experiments revealed that there is activation of the enzyme and b) the apparent maximum velocities obtained are hihgly correlated with the logarithm of the doses employed. When assayed simultaneously, both steroids were found equally active in promoting the increase in alkaliue phosphatase activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02279202
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  • 10
    Electronic Resource
    Electronic Resource
    Operator factorization on partially ordered Hilbert resolution spaces (1983)
    Springer
    Theory of computing systems 16 (1983), S. 67-77 
    Details
    ISSN: 1433-0490
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract The classical study of factorization of operators along a linearly ordered chain of orthoprojectors is extended to the more general context of partially ordered chains. With this extension the factorization theory becomes relevant to stochastic approximation, filtering and control of multidimensional systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01744570
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