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  • Artikel: DFG Deutsche Nationallizenzen  (26)
  • 2000-2004  (16)
  • 1980-1984  (10)
  • 1
    Digitale Medien
    Digitale Medien
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 88 (2000), S. 1900-1906 
    ISSN: 1089-7550
    Quelle: AIP Digital Archive
    Thema: Physik
    Notizen: The dependence of the fluorescence lifetime of the 4S3/2 state of the Er3+ ion on the ErF3 concentration was measured in fluorozirconate glasses at room temperature. Energy transfer rates were calculated from optical parameters assuming that the Er3+ ions in fluorozirconate glasses were dispersed as the cubic, bcc, or fcc structure, and were inserted into the rate equation. The dependence of the total transition rate of the 4S3/2 state of the Er3+ ion on the ErF3 concentration was calculated by using the rate equation and compared with the total transition rate measured by the lifetime experiment. Although many studies have reported the energy transfer rates in various rare-earth ions, the reliability of the calculated energy transfer rates has been hardly discussed. The energy transfer rate can be derived from the total transition rate. The energy transfer rate estimated from the rate equation calculation was compared with the energy transfer rate obtained from the lifetime measurement. It could be shown that the calculated energy transfer rate assuming that the Er3+ ions were dispersed as the cubic structure was closer to the measured energy transfer rate than that as the bcc and fcc structures. With increasing the ErF3 concentration, the calculated energy transfer rate when the Er3+ ions were dispersed as the cubic structure was comparable to the measured energy transfer rate. It was suggested that at the lower concentration of ErF3 (≤2 mol %), we needed to use the distance between Er3+ ions which was shorter than that as the cubic structure in the calculation of the energy transfer rate. © 2000 American Institute of Physics.
    Materialart: Digitale Medien
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  • 2
    Digitale Medien
    Digitale Medien
    Oxford, UK; Malden, USA : Blackwell Science Inc
    Wound repair and regeneration 12 (2004), S. 0 
    ISSN: 1524-475X
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Aim: A method for preparing acellular trachea scaffold and its effectiveness were investigated by using rabbits and dogs Methods: The sacrificed dog or rabbit trachea was collected. The spiral stent of stainless steel was inserted in the obtained trachea. To remove all of cellular components in tissues, the trachea was rinsed with sterilized 0.5% Triton X-100 for 24 to 48 hours at ambient temperature and then for removing the detergents completely using fresh water. The acellular trachea obtained was lyophilized and sterilized by ethylene oxide gas. Before implantation, the lyophilized acellular trachea was soaked in phosphate buffered saline containing 0.5% gelatin and other adhesive molecules for 2 to 18 hours at 37 degree. After 15 mm of rabbit neck trachea was removed surgically under anesthesia, the same length of reengorged acellular rabbit trachea was implanted the removed region. In the case of dog, 50 mm of thoracic trachea was removed under the mechanical ventilation and then the same length of reengorged acellular trachea was implanted by technique of end to end anastomosis. The implanted trachea was rapped by omentum. The effectiveness of acellualr scaffold on implanted-animals was evaluated by endoscope finding. Results: 1) The rabbits implanted reengorged-acellular trachea survived for minimum 10 days and maximum 60 days. It was suggested that the cause of death was the infection of implantation region. 2) The dog implanted reengorged-acellular trachea survived for over 60 days at least. The cause of death was strangulated hernia. 3) The acellular trachea containing various growth factors or cultured with fibroblasts was not always effective. Discussion: It is not necessary for the animal implanted acellular trachea to be administered the immunodepressants such as the animal implanted cryopreserved trachea. From these results, the tissue engineered acellular trachea may be more effective than the cryopreserved trachea.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Oxford UK : Blackwell Science Ltd.
    Cytopathology 13 (2002), S. 0 
    ISSN: 1365-2303
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Method of producing tissue sections from endometrial scrapings Endometrial cytology is important not only for observing cytological findings, but also for assessing histological architecture. Therefore, we attempted to produce a histological preparation from the sample using an automatic fixation apparatus (ACF 1000) which employs a membrane filter method.After observation of the cytological features, the cover slip was removed and a paraffin-embedded section was prepared.Producing a histological section from a cytological specimen prepared with the ACF 1000 apparatus was more useful and easier than with the techniques described to date. Peelability of the cell was avoided by using a silane-coated membrane filter. Observation of the same cell cluster as that observed in the cytological sample was also facilitated in the histological section by this technique.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Science Ltd
    Alimentary pharmacology & therapeutics 14 (2000), S. 0 
    ISSN: 1365-2036
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Background: Alkaline oesophagitis attributable to duodenal mechanisms may induce oesophageal carcinogenesis in a rat reflux model. Aim: To investigate the mechanism of the regurgitation after distal partial gastrectomy. Methods: Oesophageal manometry was used in 16 patients before and after distal partial gastrectomy with reconstruction by Bilroth methods. Serum concentrations of four gastrointestinal hormones were measured by radioimmunoassay in 10 gastrectomy patients and nine healthy volunteers before and after a standardized meal. Results: The lower oesophageal sphincter pressure was reduced to 83% after surgery. The amplitude and duration of the peristaltic waves tended to be increased, and the velocity tended to be less after surgery (amplitude 120%, duration 114%, velocity 88%). Interrupted waves appeared more frequently after surgery. The manometric changes in gastrectomized patients are considered to be disadvantageous relative to regurgitation. After surgery, gastrin and pancreatic polypeptide were completely abolished postprandially, whereas cholecystokinin and neurotensin were significantly increased. Conclusion: The hormonal changes should have a suppressive effect on the lower oesophageal sphincter. Both the manometric and the hormonal changes may exacerbate reflux oesophagitis after distal partial gastrectomy.
    Materialart: Digitale Medien
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  • 5
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] The observation was carried out with an X-ray burst monitor which consists of two sets of rotating modulation collimators: two coarse modulation collimators (CMC 1,2) and a fine modulation collimator (FMC 1) with a field of view of 17.4 and 5.8 (FWHM), respectively. Positions of steady sources and ...
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    International journal of legal medicine 113 (2000), S. 201-207 
    ISSN: 1437-1596
    Schlagwort(e): Key words Methamphetamine ; Cardiotoxicity ; Cardiomyocyte ; Cell culture ; Hypertrophy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Notizen: Abstract To investigate the direct effects of methamphetamine (MAP) on cardiac lesions seen in MAP abusers, isolated adult rat ventricular cardiomyocytes (ARCs) were exposed to MAP (0.05–1.0 mM) in medium 199 containing 10% fetal calf serum. Isolated ARCs attached to laminin-coated substrata and began to spread into polygonal shapes with pseudopodia at day 6 in normal culture. However, the cell attachment and spreading were inhibited by exposure to MAP (0.5 and 1.0 mM) for the first 7 days in culture. On the other hand, exposure to MAP (0.05 and 0.1 mM) for 7 days after a 6-day period of normal culture, led to a larger cross surface area of cells with more abundant actin bundles compared to control cells (p 〈 0.05). This development of spreading area resembled that of norepinephrine-treated ARCs. In addition, immunoreactive atrial natriuretic peptide (ANP) granules developed and accumulated around the nuclear region of ARCs exposed to MAP and the number of ANP positive cells tended to increase in a dose-dependent manner. These results suggest that chronic exposure to a high concentration of MAP may directly inhibit development of ARCs in culture and that a continuous exposure to a low concentration of MAP may facilitate the development of cellular hypertrophy. Therefore, hypertrophied cardiomyocytes in MAP abusers may be provoked by multifactorial incidents of direct and indirect actions of MAP.
    Materialart: Digitale Medien
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  • 7
    ISSN: 1437-1596
    Schlagwort(e): Key words Sudden infant death syndrome (SIDS) ; Fetal ; haemoglobin ; Rat globin ; High performance liquid chromatography (HPLC) ; Extramedullary haematopoiesis (EMH)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Rechtswissenschaft
    Notizen: Abstract The influence of repeated hypoxia on the development of haemoglobin (Hb) subtypes and on extramedullary haematopoiesis (EMH) was investigated in young Wistar rats of different ages. The rats were exposed to hypercapnic/hypoxic and to “simple” hypoxic conditions. The results obtained were compared to those of an untreated age-matched control group. Different globin chains were measured using HPLC and time-of-flight (TOF) mass analysis. The number of EMH cells was evaluated by cell counting. By determining the proportions of α- and β-chains, fetal, neonatal and mature types of globin chain composition could be differentiated. The β-2 chain levels were significantly higher in hypercapnic/hypoxic environments than in the controls and simple hypoxic environments. The numbers of EMH cells in the two groups subjected to hypercapnia/hypoxia decreased significantly more slowly compared to the controls and simple hypoxia groups. Therefore, the development of Hb subtypes and the EMH activity in rats were influenced by both repeated hypercapnia and hypoxia.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Astrophysics and space science 103 (1984), S. 345-352 
    ISSN: 1572-946X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Two successive X-ray outbursts of the recurrent X-ray pulsar GX301-2 were observed in April and May, 1982 from Hakucho. Apart from general increase during flares lasting for several days, the X-ray intensity was highly variable on a time-scale of the order of an hour. The outstanding feature is that the amplitude of 700 s pulsation of GX301-2 changes rapidly in a very wide range. When the pulsation is distinct, the depth of modulation is as large as 60%; whereas, the pulse amplitude occasionally diminishes almost to an undetectable level so that the observed X-rays become unpulsed. Such drastic changes are found to occur at any intensity levels observed. In several cases of abrupt changes of pulse amplitude, the intensity at the pulse bottom remained essentially unchanged. These properties suggest a hypothesis that the X-ray emission from GX301-2 consists of a pulsating component with variable amplitude and a non-pulsating component of less variable intensity.
    Materialart: Digitale Medien
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  • 9
    ISSN: 1435-1463
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary The three-dimensional distribution of vasoactive intestinal polypeptide (VIP)-like immunoreactive (VIPLI) structures in rat stomach and their origins were investigated using the indirect immunofluorescence method in whole mounted tissue. The present study demonstrates a very dense VIPLI fiber meshwork in the circular muscle layer, longitudinal muscle layer and myenteric plexus. In the muscle layers VIPLI immunoreactive fibers run parallel to the muscle. VIPLI fibers are distributed evenly throughout the entire stomach. We have also shown by experimental manipulations that the fibers in the stomach originate from VIPLI neurons in the myenteric plexus.
    Materialart: Digitale Medien
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  • 10
    ISSN: 1437-7772
    Schlagwort(e): Key words Granulocyte colony stimulating factor ; G-CSF-producing esophageal carcinoma ; Serum G-CSF level ; Adjuvant chemo-radiotherapy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We report here a case of granulocyte-colony stimulating factor (G-CSF)-producing esophageal carcinoma in a 66-year-old man. The International Union Against Cancer (UICC) staging was IV A, and a diagnosis of moderately differentiated squamous cell carcinoma was made, based on histopathology. The diagnosis was based on marked leukocytosis (41 500 leukocytes/mm3) and an elevated serum level of G-CSF (154 pg/ml). Immunofluorescent histochemistry and northern blot analysis confirmed the expression of G-CSF protein in cancer cells and its mRNA in cancer tissue. We paid special attention to any change in serum G-CSF levels during aggressive cancer treatment. Subtotal esophagectomy induced a significant decrease in G-CSF level. Adjuvant chemo-radiotherapy, targeting celiac lymph node metastasis, and radiotherapy, targeting solitary lung metastasis, together effectively maintained a low serum G-CSF level, despite a recurrence of the tumor in the lungs, in the form of multiple metastases, with an increase in serum G-CSF levels. The patient's clinical course suggested that serum G-CSF would be a useful marker for monitoring the effects of treatment on G-CSF-producing carcinoma.
    Materialart: Digitale Medien
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