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  • 1
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Freshwater mud eel, Amphipnous cuchia, were injected intraperitoneally daily with 100 ng of vitamin D3/100 g body weight and maintained in media containing either no calcium or different calcium concentrations. The eels were killed after 1, 3, 5, 10 and 15 days following the treatment and their serum calcium levels were measured. The ultimobranchial glands were fixed and processed using the routine paraffin method for histological studies. The results of the present study indicate that vitamin D3 can induce hypercalcaemia in eels kept in different calcium environments. Also, the ultimobranchial glands became hyperactive following vitamin D3 treatment. It is concluded that in mud eels, the gland has a calcium-regulating function.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science, Ltd
    Plant, cell & environment 24 (2001), S. 0 
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Seedlings of Arabidopsis thaliana were treated with Cd, and transcript populations that changed their levels were screened by a fluorescent differential display method. Among the 170 cDNAs initially identified, 31 were further characterized for their identity, expression profile and response to other stresses. Sequencing revealed 10, 12 and 15 to be related to signal transduction, protein denaturing stress and responses to active oxygen species, respectively. Many of these genes responded not only to Cd, but also to oxidative stress, Cu ions and a protein denaturation agent. Upon introduction into yeast cells, two genes, encoding ATMEKK1 and a putative farnesylated protein that has two metal binding motifs, endowed marked toleration of Cd toxicity. These results suggest that oxidative stress and protein denaturation are important components of Cd toxicity, and that to cope with such stresses, plants activate a set of genes involved in metal detoxification, protein refolding and wound healing. The results also suggested temporarily and spatially well-regulated protein phosphorylation and activation of transcription factors, accompanied by their transcription.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1365-2516
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We describe successful induction of immune tolerance (IT) in a 10-month-old boy with severe haemophilia B. Urticaria developed soon after starting prophylactic treatment and was associated with an inhibitor at 7 Bethesda units mL−1. Initially, we tried low dose factor IX therapy to induce IT with only a transient effect. The patient experienced an intracranial haemorrhage. A simple bolus dose of FIX eradicated the inhibitor. Thereafter he has been free from inhibitor and nephrotic syndrome for more than 5 years, although he receives FIX three times a week.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Two feeding experiments were conducted to evaluate the ability of rainbow trout, Oncorhynchus mykiss (Walbaum), to discriminate the sufficiency of a single essential amino acid in diets using self-feeders. In experiment 1, 12 groups of 23–24 fish (initial weight; 25.3 ± 0.5 g) were acclimated to a casein-based low protein diet (crude protein, CP = 10%) for 2 weeks. They were then offered for 32 days a combination of two wheat gluten-based diets fortified by crystalline amino acids (CP = 40%), with a complete amino acid composition (complete) and a composition deficient in either methionine (Met-def) or lysine (Lys-def) (six groups per treatment). In the complete and Met-def diet selection groups, no specific preference for either of the diets was observed. However, in the complete and Lys-def diets selection groups, a preference for the complete diet increased gradually (P 〈 0.05, for the last 8-day period). In experiment 2, 12 groups of 21–22 fish (initial weight; 27.6 ± 0.5 g) were acclimated to a casein-based high protein diet (CP = 40%) for 2 weeks, and then the complete diet and one of the amino acid-deficient diets were offered alternately for 8-day intervals over 32 days (six groups per treatment). In the complete–Met-def diet groups, overall daily feed consumption (g tank−1) increased gradually, but that for the Met-def diet seemed nearly stable. In the complete–Lys-def diet group, feed consumption when the complete diet was offered increased, while it then decreased after the complete diet was replaced with the Lys-def diet. These results indicate that trout discriminate sufficiency of lysine in diets and regulate their feed consumption.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The influence of dietary fat level and whole-body adiposity on voluntary energy intake of juvenile rainbow trout Oncorhynchus mykiss (Walbaum) was examined using self-feeders. Groups of lean fish [crude fat (CF) = 7%] and fat fish (CF = 11%), pretreated with a commercial diet with or without supplemental pollock oil, were self-fed one of three fat level diets (CF = 8%, 13.5% and 19%) for 48 days at 17 °C. Final body weight (BW) and total digestible energy (DE) intake (kJ per fish) were positively affected by the initial BW. Relative to the initial BW, however, fat fish consumed less DE than lean fish. Although the effect of dietary fat level was not significant, percentage weight gain and daily DE intake per BW (kJ kg−1 BW day−1) of fat fish were significantly lower than those of lean fish (ancova with initial BW as a covariate, P 〈 0.05). Energy digestibility, feed efficiency and protein retention were improved with the dietary fat level; however, there was no difference resulting from body fat level. The whole-body fat levels at the end of the experiment increased with the dietary fat level. Between groups self-fed the same diet, fat levels of the initially fat fish were still higher than those of the lean fish. The results of the present medium-term study suggest that rainbow trout adjust DE intake from diets with fat levels ranging from 8% to 19%. Although body fat level affects neither energy digestibility nor protein utilization, a high body fat level may reduce DE intake and consequently depress growth.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science, Ltd
    Aquaculture research 32 (2001), S. 0 
    ISSN: 1365-2109
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Growth performance of rainbow trout fry initially weighing 0.27 ± 0.09 g fed on demand by self-feeders and fed continuously by an automatic feeder was compared. Each group of 50 fish (five replicates per treatment) was fed a commercial trout feed for 44 days with a self-feeder that consisted of a highly sensitive switch and a feeder; or with an automatic feeder with two feeding schedules, 12 h (during light phase) per day and 24 h per day. The self-feeders were adjusted to release 0.02 g of food at each switch actuation. Each automatic feeding group was initially fed 0.7 g per day (5.1% of initial biomass) and this was increased twofold and threefold on days 21 and 32 respectively. Almost all feeding activity of the self-feeding groups was observed during the light phase. There was no significant effect of the feeding strategy or the feeding schedule on feed efficiency and specific growth rate, but mortality (including cannibalism) and the coefficient of variation of final fish weigh were significantly lower in the self-feeding groups. It is concluded that rainbow trout fry feed efficiently to attain growth identical to an automatic feeding fish and grow more homogeneously with self-feeders.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Th1 and Th2 cells, resulting from antigenic stimulation in the presence of IL-12 and IL-4, respectively, are implicated in the pathology of various diseases including allergic and autoimmune diseases. Txk/Rlk is a member of Tec family tyrosine kinases. We reported that Txk acts as a Th1-specific transcription factor in the T lymphocytes.Objective In this study we have asked whether administration of txk expression plasmid brings about a Th1/Th2 shift in vivo of the mice, and subsequent reduction of circulating IgE.Methods Mice were administered a txk expression plasmid with hemagglutinating virus of Japan (HVJ) envelope vector. Txk expressions in spleen cells were assessed by immunoblotting and immunocytochemical staining. Cytokine productions by the spleen cells and serum Ig concentrations were studied by ELISA.Results Administration of a txk expression plasmid with HVJ vector induced expression of Txk in the spleen cells. The spleen cells showed enhanced Th1-specific cytokine production; spleen cells from the txk administered mice produced more IFN-γ as compared with those from control plasmid-administered mice in an antigen-specific manner. IL-2 and IL-4 secretions of the spleen cells were comparable between the two mouse groups. Txk administration did not reduce serum IgG concentration. It markedly reduced total IgE level and an IgG1/IgG2a ratio, reflection of Th1/Th2 balance, in sera. Furthermore, txk administration reduced ovalbumin (OVA)-specific IgE levels in sera of the OVA sensitized mice.Conclusion Thus, Txk enhances IFN-γ secretion and thus modulates Th1/Th2 cytokine balance, leading to reduction of serum IgE.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Oxford, UK; Malden, USA : Blackwell Science Ltd
    Journal of fish biology 65 (2004), S. 0 
    ISSN: 1095-8649
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The Mediterranean turbot Psetta maxima consists of two main genetically distinct lineages (western Mediterranean and ‘eastern secluded Mediterranean’ basins) as investigated by mitochondrial DNA analysis. Within the latter lineage, most haplotypes from the Sea of Azov were endemic and more than half of them derived from a single ancestral haplotype shared among all the eastern Mediterranean areas. There was no relation between morphotype variation in bony tubercles and mitochondrial genealogy.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 68 (2003), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: : Polyphenol oxidase (PPO) was purified and characterized from bean sprouts by ammonium sulfate precipitation, DEAE-Toyopearl 650M, CM-Toyopearl 650M, SuperQ-Toyopearl 650S and QAE-Toyopearl 550C column chromatographies. Substrate staining of the crude extract on electrophoresis showed the presence of 2 isozymic forms of this enzyme. The molecular weight of the purified enzyme was estimated to be about 54 kDa. The optimum pH was 9.0 and optimum temperature 40 °C. Heat inactivation occurred about 30 °C. PPO showed activity to catechol, pyrogallol and dopamine. These compounds such as ascorbic acid, L-cysteine, 2-mercaptoethanol, and glutathione used was the effective inhibitor. Enzyme activity was maintained for 7 d at 4 °C but suddenly decreased after 8 d.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 144 (2001), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background  Synthesis of cysteinyl leukotrienes (LTs) is known to play a part in the pathogenesis of inflammatory diseases. Objectives  To define the involvement of cysteinyl LTs in atopic dermatitis (AD). Methods  Synthesis of cysteinyl LTs was assessed in patients with AD and healthy volunteers by measuring urinary LTE4, a useful index of systemic cysteinyl LT synthesis, using liquid chromatography/tandem mass spectrometry. Results  Mean ± SD urinary LTE4 levels in patients with AD (125 ± 69 pg mg−1 creatinine, n = 20) were significantly higher (P 〈 0·01) than in healthy volunteers (60 ± 19 pg mg−1 creatinine, n = 17). A significant correlation between urinary LTE4 and total serum IgE levels in patients with AD was observed (r = 0·643, P 〈 0·05). Conclusions  Our findings demonstrate an enhanced synthesis of cysteinyl LTs in patients with AD and suggest that cysteinyl LTs are involved in the pathophysiology of AD.
    Type of Medium: Electronic Resource
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