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  • 1
    ISSN: 1432-1440
    Keywords: Key words Antibody ; Capsid antigens ; HHV-8 ; Kaposi’s sarcoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Sequences of a new herpesvirus with homology to gammaherpesvirinae were recently identified in AIDS-associated Kaposi’s sarcoma (KS). Subsequently this novel virus, called KS-associated virus (KSHV) or human herpesvirus (HHV) 8 was detected in classical KS and AIDS-associated body cavity based lymphomas by polymerase chain reaction. In this report major and minor capsid proteins of HHV-8 were molecularly cloned and produced as recombinant proteins in Escherichia coli. Sera from 69 HIV-1 infected patients with KS, 30 HIV-1 infected patients without KS and 106 control individuals were tested by enzyme-linked immunosorbent assay for anti-HHV-8 capsid IgM and IgG antibodies. Sera from four patients were tested over periods ranging from 18 months to 6 years. IgG antibodies directed against HHV-8 capsid antigens were detected in patients with AIDS-associated KS and in some AIDS patients without KS. Seroconversion with IgM and IgG antibodies directed against HHV-8 capsid proteins occurred more than 1 year prior to diagnosis of KS. In a considerable portion of KS patients no IgM or IgG antibodies against HHV-8 capsid proteins were detected. In these patients there was an inverse relationship between antibodies against HHV-8orf26 and the CD4/CD8 ratio, suggesting that the inconsistency of anti-HHV-8orf26 antibodies is due at least partly to an impaired immune response. No reactivity against HHV-8 capsid antigens was detected in the vast majority of sera from HIV-negative control individuals. Our findings indicate that a specific humoral immune response against capsid proteins is raised in HHV-8 infected individuals, and that anti-capsid antibodies can be used to diagnose HHV-8 infection. The correlation between occurrence of anti-HHV-8 antibodies and KS supports the hypothesis of a causative role of HHV-8.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1076
    Keywords: Term newborns ; Dyspnoea ; Volume expansion ; Bicarbonate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Abstract In a prospective, randomised, open trial 103 term newborns with persisting dyspnoea, tachypnoea and/or cyanosis were treated with oxygen for 5–10 min and then with oxygen plus mask continuous positive airway pressure (CPAP) for another 5–15 min. Cases with overt prenatal or intrapartum obstetric pathology had been excluded from the study. Forty-one infants (40%) responded to this procedure within 10–25 min. The remaining 62 infants (60%) were randomly allocated to one of three forms of further treatment: continuation of mask CPAP for 20 min (group A,n=24), volume expansion with 9 ml of 3 ml albumin, 3 ml glucose, and 3 mEq of sodium bicarbonate (group B,n=24), or volume expansion with 4.5 ml albumin and 4.5 ml glucose (group C,n=14). There was no statistical difference in birth weight, gestational age or Apgar scores at 1 and 5 min between the infants of the groups. Time to normalisation of symptoms was significantly shorter in the volume expansion groups (B: 45±41 min, range 20–180, and C: 80±72 min, range 20–210) than in the mask CPAP group (A: 224±256 min, range 30–1200,P=0.02). There were statistical differences in umbilical cord and capillary pH values among the infants of the three groups, but the response to therapy was not related to the degree of acidaemia. Thirty-four infants (33%) who did not respond were admitted to a special care unit for further examination (group A: 21/24, group B: 7/24; group C: 6/14). Of these, 23 had no abnormal findings, 8 infants had radiological signs of transitory respiratory distress, and 1 had a nontension pneumothorax. Septicaemia was found in two infants. No infant was intubated. At discharge all 103 infants did well. Conclusion Incremental application of simple primary care procedures including volume expansion (with or without alkali) in term newborns with persisting postnatal tachypnoea and dyspnoea helps avoid overtreatment and unnecessary separation from the mothers in most cases and reliably selects infants who need close monitoring or special treatment.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-1076
    Keywords: Key words Term newborns ; Dyspnoea ; Volume expansion ; Bicarbonate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In a prospective, randomised, open trial 103 term newborns with persisting dyspnoea, tachypnoea and/or cyanosis were treated with oxygen for 5–10 min and then with oxygen plus mask continuous positive airway pressure (CPAP) for another 5–15 min. Cases with overt prenatal or intrapartum obstetric pathology had been excluded from the study. Forty-one infants (40%) responded to this procedure within 10–25 min. The remaining 62 infants (60%) were randomly allocated to one of three forms of further treatment: continuation of mask CPAP for 20 min (group A, n = 24), volume expansion with 9 ml of 3 ml albumin, 3 ml glucose, and 3 mEq of sodium bicarbonate (group B, n = 24), or volume expansion with 4.5 ml albumin and 4.5 ml glucose (group C, n = 14). There was no statistical difference in birth weight, gestational age or Apgar scores at 1 and 5 min between the infants of the groups. Time to normalisation of symptoms was significantly shorter in the volume expansion groups (B: 45 ± 41 min, range 20–180, and C: 80 ± 72 min, range 20–210) than in the mask CPAP group (A: 224 ± 256 min, range 30–1200, P = 0.02). There were statistical differences in umbilical cord and capillary pH values among the infants of the three groups, but the response to therapy was not related to the degree of acidaemia. Thirty-four infants (33%) who did not respond were admitted to a special care unit for further examination (group A: 21/24, group B: 7/24; group C: 6/14). Of these, 23 had no abnormal findings, 8 infants had radiological signs of transitory respiratory distress, and 1 had a non-tension pneumothorax. Septicaemia was found in two infants. No infant was intubated. At discharge all 103 infants did well. Conclusion Incremental application of simple primary care procedures including volume expansion (with or without alkali) in term newborns with persisting postnatal tachypnoea and dyspnoea helps avoid overtreatment and unnecessary separation from the mothers in most cases and reliably selects infants who need close monitoring or special treatment.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1471-0528
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Objective To define the normal ranges of umbilical cord blood oxygen saturation (SaO2) and acid-base status at birth and to evaluate the effect of gestational age on cord blood values in vigorous newborn infants following spontaneous vaginal birth from a vertex position.Design Prospective study.Setting Department of Obstetrics and Gynaecology, University of Graz, Austria.Sample Cord blood samples from 1281 vigorous newborn infants.Methods Cord blood sampling was performed following on newborn infants following spontaneous vaginal birth in a vertex position. SaO2 was measured directly by a spectrophotometer and pH, base excess, pCO2 and pO2 by a pH/blood-gas analyser. Infants with a 5-minute Apgar score ≥ 7 were considered vigorous. Subgroups were classified according to the gestational age: preterm, term and postterm (〈 37, 37–42 and 〉 42 weeks, respectively).Results The median umbilical artery SaO2 was 24.3% and the 2.5th centile was as low as 2.7%. The median umbilical artery values were pH = 7.25, base excess =−4.3 mmol/L and pO2= 16 mmHg. The 2.5th centiles were 7.08, −11.1 mmol/L and 5 mmHg, respectively. The median umbilical artery pCO2 was 50 mmHg and the 97.5th centile was 75 mmHg. The mean umbilical artery and vein SaO2 values were not significantly influenced by gestational age. The umbilical artery SaO2 and base excess values were strongly skewed. The mean umbilical artery pH values in preterm infants were higher than in other subgroups. The mean umbilical artery and vein base excess values were lower in post-term newborn infants than in other subgroups.Conclusions The physiological range of oxygen saturation in umbilical cord of vigorous newborn infants at birth is wide and skewed. In contrast to pH and base excess, umbilical cord blood oxygen saturation is not influenced significantly by gestational age at birth.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Suite 500, 5th Floor, 238 Main Street, Cambridge, Massachusetts 02142, USA : Blackwell Science Inc.
    International journal of gynecological cancer 6 (1996), S. 0 
    ISSN: 1525-1438
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Whether the size of a retroperitoneal lymph node reflects its status is not clear. We measured the size of 125 positive and 160 negative pelvic lymph nodes in 32 consecutive patients with node-positive endometrial cancer. The measurements were compared with those of 143 pelvic lymph nodes of five randomly selected patients with endometrial cancer without node involvement. Overall, positive lymph nodes were larger than negative lymph nodes in both node-positive patients and node- negative controls (P 〈 0.01). There was a positive correlation between the size of positive lymph nodes and the size of the metastasis therein (P 〈 0.01). However, 68 of 125 (54%) positive lymph nodes measured less than 10 mm in maximum diameter, while 46 of 160 (29%) negative lymph nodes in node-positive patients measured more than 10 mm in maximum diameter. The metastasis was detected in more than 50% of step-serial sections in only 74% of positive lymph nodes. These data suggest that the size of a lymph node does not reliably reflect its status. Thus, these nodes may be missed if only enlarged nodes are removed. If only one section of a lymph node is performed, at least 26% of metastases will be missed.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 782 (1996), S. 0 
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford [u.a.] : International Union of Crystallography (IUCr)
    Acta crystallographica 54 (1998), S. 952-954 
    ISSN: 1600-5759
    Source: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Topics: Chemistry and Pharmacology , Geosciences , Physics
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1437-3262
    Keywords: Key words Structural units ; Structure evolution ; Periadriatic Lineament ; Pannonian Basin ; Hungary ; Slovenia ; Croatia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract Due to the political boundaries between the Central European countries, on one hand, and the thick Tertiary cover in the Pannonian Basin, on the other, the eastward continuation of the Alpine and Dinaridic units has been ambiguous and poorly documented. Based on comparative analyses, the aim of the present paper is to define the pre-Tertiary structural units in the junction area of the Alpine, Dinaridic, and Pannonian regions, in the SW part of the Pannonian Basin, and to draw conclusions on the continuation of the Alpine and Dinaridic units. According to diagnostic characteristics of the Periadriatic Lineament system, the Balaton Lineament system may be considered as its direct eastern continuation. North of the Periadriatic–Balaton Lineament system, the Transdanubian Range Unit, due to its pre-Tertiary paleogeographic setting, shows mainly South Alpine facies relations; however, its present structural position is identical to that of the Upper Austroalpine nappes. Between the Periadriatic–Balaton and Zagreb–Zemplin Lineament systems heterogeneous structural units are juxtaposed, forming the Sava Composite Unit. In the northern part of this composite unit non-metamorphosed nappes occur which can be considered the eastern continuation of the South Alpine units. These nappes are overthrust onto Internal Dinaridic units in the Tertiary. The Zagreb–Zemplin (Mid-Hungarian) Lineament separates the Sava Unit from the Tisza Unit showing close affinity to the Tethyan margin of the Eurasian plate during the early stage of the Alpine evolution.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1435-1285
    Keywords: Key words Risk stratification – acute myocardial infarction – magnetocardiography – late fields – late potentials ; Schlüsselwörter Risikostratifizierung – akuter Myokardinfarkt – Magnetokardiographie – Spätfelder – Spätpotentiale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In der Risikostratifizierung für einen plötzlichen Herztod bzw. das Auftreten eines arrhythmogenen Ereignisses nach akutem Myokardinfarkt (AMI) wurden bisher eine hohe Spezifität und ein hoher negativ prädiktiver Wert durch Kombination mehrerer Methoden erreicht. Sensitivität und positive Prädiktion sind jedoch weiterhin nicht ausreichend. Wie der Elektro- liegen der Magnetokardiographie (MKG) dieselben physiologischen Prozesse zugrunde, wobei die Signale der jeweiligen Methode jedoch unterschiedliche Informationen beinhalten. Methodik: Wir untersuchten bei 50 Patienten nach AMI und 32 Probanden die von der Erregungsausbreitung erzeugten Magnetfelder und die daraus analog zu der Spätpotential(LP)analyse ermittelten Spätfelder (LF). Wir definierten anhand der 95%-Konfidenzintervalle Normbereiche für herzgesunde Probanden (QRS (Dauer des gesamten QRS-Komplexes) 〈 = 97 ms, RMS 〉 = 0,6 (Maß für die Fläche des terminalen QRS-Komplexes), LAS (Dauer des terminalen QRS-Komplexes) 〈 25 ms). Ergebnisse: Wir verglichen mittels der erstellten Normwerte die Befunde der LF- mit denen der LP-Messung hinsichtlich pathologisch-nicht patholotisch und fanden bei 76% der Patienten eine Übereinstimmung. Bei 4 Patienten waren im ST-Segment im Gegensatz zum EKG im MKG niedrigamplitudige Signale nachweisbar, während bei 6 Patienten, deren Befund “Spätpotential positiv” nur auf RMS und LAS beruht, im MKG keine Spätfelder gefunden werden konnten. Bei der Normwerterstellung zeigte sich, daß der magnetische QRS-Komplex kürzer als der elektrische ist. Diskussion: Insgesamt liefert die LF-Berechnung ähnliche Ergebnisse wie die LP-Berechnung. Vermutlich existieren magnetisch detektierbare intrakardiale Erregungskreisläufe, die im oberflächlich abgeleiteten EKG keine Potentialdifferenz erzeugen. Welche der beiden Methoden letztendlich die höhere Sensitivität bzw. Spezifität zeigt, bleibt in weiteren Studien mit Hoch-Risikogruppen zu untersuchen.
    Notes: Summary A high specificy and a high positive prediction has been reached in risk stratification for a sudden cardiac death after acute myocardial infarction (AMI) by combining multiple methods. But sensitivity and negative prediction are still not satisfying. There are the same physiological processes underlying magnetcardiography (MCG) and electrocardiography (ECG). Nevertheless, the signals in each method contain different information. Methods: We studied the cardiac magnetic fields in 50 patients after AMI and in 32 probands and calculated the magnetic late field (LF), according to Simsons late potential (LP) analysis. We defined normal values, according to the 95% confidence interval of the probands (QRS 〈 =97 ms, RMS 〉 = 0.6, LAS 〈 25 ms). Results: We compared the results of LF and LP analysis regarding pathologic-nonpathologic and found 76% of the patients with the same results in both methods. Four patients had magnetic signals with low amplitude in the ST segment in contrast to the ECG result, while 6 patients with a “LP positive” diagnosis based on RMS and LAS only, did not show LF. In addition, we have found the magnetic QRS complex to be shorter than the electrical one. Discussion: In general, the results of LF measurement are similiar to the ones of LP measurement. Presumably, there are intracardial currents, which are not detectable by ECG. Further studies are needed to evaluate the prognostic value in patients at high risk for cardiac arrhythmias.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1433-0407
    Keywords: Schlüsselwörter Tuberkulöse Meningitis ; Polymerase- Kettenreaktion ; Liquor ; Mycobacterium tuberculosis ; Mycobacterium fortuitum ; Key words Tuberculous meningitis ; Polymerase chain reaction ; Mycobacteria tuberculosis ; Mycobacteria fortuitum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: , alsein ubiquitärer Keim mit geringer Pathogenität, Untersuchungsproben verunreinigen kann und die Verwendung von Primerpaaren, die gruppenspezifische mykobakterielle DNA erkennen, zu falsch-positiven Resultaten führen kann. Für die Validität der PCR ist daher neben der Sensitivität der Nachweisreaktion, die Spezifität der eingesetzten PCR-Primer von großer Bedeutung, um zwischen mykobakteriellen Subtypen zu differenzieren.
    Abstract: Value of the polymerase chain reaction (PCR) for diagnosing tuberculous meningitis
    Notes: Zusammenfassung
    Type of Medium: Electronic Resource
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