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  • 1
    Digitale Medien
    Digitale Medien
    Cryopreservation of zygotic embryos of a Japanese terrestrial orchid (Bletilla striata) by vitrification (1997)
    Springer
    Plant cell reports 16 (1997), S. 754-757 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key wordsBletilla striata ; Cryopreservation ; Embryo ; Orchid ; Vitrification
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The seeds of a Japanese terrestrial orchid (Bletilla striata Rchb.f.) were germinated and cultured on solidified new Dogashima (ND) medium for 10 days. These embryos were then precultured on ND medium supplemented with 0.3 m sucrose for 3 days at 25°C in continuous dark. The embryos were then overlaid with a mixture of 2 m glycerol and 0.4 m sucrose for 15 min at 25°C and finally dehydrated with highly concentrated vitrification solution (PVS2) for 3 h at 0°C prior to immersion into liquid nitrogen for 30 min. After rapid warming, the embryos were washed with liquid ND medium supplemented with 1.2 m sucrose for 20 min and then plated on ND medium. Successfully vitrified and warmed embryos developed into normal plantlets. The rate of plant regeneration amounted to about 60%. This vitrification method appears to be a promising technique for cryopreservation of orchids.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990050314
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  • 2
    Digitale Medien
    Digitale Medien
    Cryopreservation of in vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott) by vitrification. 1. Investigation of basic conditions of the vitrification procedure (1997)
    Springer
    Plant cell reports 16 (1997), S. 594-599 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words Shoot tips ; Cryopreservation ; Vitrification ; Taro [Colocasia esculenta (L.) Schott.] ; Tropical crops
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In vitro-grown shoot tips of taro (Colocasia esculenta (L.) Schott.) were successfully cryopreserved by vitrification. Excised shoot tips precultured on solidified MS supplemented with 0.3 M sucrose and maintained under a 16 h phtoperiod at 25°C for 16 h were loaded with a mixture of 2 M glycerol plus 0.4 M sucrose for 20 min at 25°C. The shoot tips were then sufficiently dehydrated with a highly concentrated vitrification solution (PVS2) for 20 min at 25°C prior to immersion into liquid nitrogen. Successfully vitrified and warmed shoot tips resumed growth within 7 days and developed shoots directly without intermediate callus formation. The average rate of shoot recovery amounted to around 80%, and the vitrification protocol appeared to be very promising for the cryopreservation of taro germplasm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990050285
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  • 3
    Digitale Medien
    Digitale Medien
    Amyloplast formation in cultured tobacco cells. III Determination of the timing of gene expression necessary for starch accumulation (1999)
    Springer
    Plant cell reports 18 (1999), S. 589-594 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words ADP-glucose starch glycosyl transferase ; Amyloplast ; BY-2 ; Nicotiana tabacum ; Transcription/translation inhibitors
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract When BY-2 cultured tobacco (Nicotiana tabacum L.) cells were transferred to auxin-depleted culture medium containing cytokinin (benzyladenine, 1 mg/l), the starch content per cell started increasing from 18 h of culture and amyloplasts had formed by 48 h. Pulse-treatment of the cells with actinomycin D and cycloheximide for the first 12 h (or longer) of culture significantly decreased the cellular starch content after 48 h, whereas the starch content did not decrease significantly when the cells were released from the inhibition within 6 h. This suggests that nuclear gene expression necessary for amyloplast formation begins 6–12 h after the transfer. Immunoblotting analysis of the accumulation of ADP-glucose starch glycosyl transferase (starch synthase) supported this inference.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990050627
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  • 4
    Digitale Medien
    Digitale Medien
    Cryopreservation of encapsulated shoot primordia induced in horseradish (Armoracia rusticana) hairy root cultures (1997)
    Springer
    Plant cell reports 16 (1997), S. 469-473 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words Cryopreservation ; Encapsulation-dehydration ; Encapsulation-vitrification ; Hairy roots ; Horseradish shoot primordia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Shoot primordia induced in Armoracia rusticana Gaertn. Mey. et Scherb. (horseradish) hairy root cultures were successfully cryopreserved by two cryogenic procedures. Encapsulated shoot primordia were precultured on solidified Murashige-Skoog medium supplemented with 0.5 M sucrose for 1 day and then dehydrated with a highly concentrated vitrification solution (PVS2) for 4 h at 0°C prior to a plunge into liquid nitrogen. The survival rate of encapsulated vitrified primordia amounted to 69%. In a revised encapsulation-dehydration technique, the encapsulated shoot primordia were precultured with a mixture of 0.5 M sucrose and 1 M or 1.5 M glycerol for 1 day to induce dehydration tolerance and then subjected to air-drying prior to a plunge into liquid nitrogen. The survival rate of encapsulated dried primordia was more than 90%, and the revived primordia produced shoots within 2 weeks after plating. A long-term preservation of shoot primordia was also achieved by the technique. Thus, this revised encapsulation-dehydration technique appears promising as a routine method for the cryopreservation of shoot primordia of hairy roots.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01092768
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  • 5
    Digitale Medien
    Digitale Medien
    Cryopreservation of in vitro-grown axillary shoot-tip meristems of mint (Mentha spicata L.) by encapsulation vitrification (1999)
    Springer
    Plant cell reports 19 (1999), S. 150-155 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Key words Cryopreservation ; Encapsulation-vitrification ; Meristems ; Mint ; Vitrification
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Alginate-coated meristems from in vitro-grown axillary buds of mint (Mentha spicata L.) were successfully cryopreserved by vitrification. Excised meristems from nodal segments cold hardened at 4  °C for 3 weeks were encapsulated and osmoprotected by a mixture of 2 M glycerol plus 0.4 M sucrose. These meristems were dehydrated with a highly concentrated vitrification solution (PVS2 solution) for 3 h at 0  °C prior to a plunge into liquid nitrogen. Successfully encapsulated vitrified meristems developed shoots within a week after plating without intermediary callus formation. The average rate of shoot formation amounted to nearly 90%. This procedure was successfully applied to other Mentha species. It was also confirmed that encapsulated vitrified meristems produced a much higher rate of shoot formation than the encapsulated dried meristems. Thus, this revised encapsulation vitrification method appears promising for the cryopreservation of mint and other germplasm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002990050725
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  • 6
    Digitale Medien
    Digitale Medien
    Survival of cultured cells and somatic embryos of Asparagus officinalis cryopreserved by vitrification (1989)
    Springer
    Plant cell reports 8 (1989), S. 418-421 
    Details
    ISSN: 1432-203X
    Schlagwort(e): Cryopreservation ; Vitrification ; Plant germplasm ; Asparagus ; Asparagus officinalis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Cultured cells and somatic embryos derived from the mesophyll tissue of asparagus (Asparagus officinalis L.) were cryopreserved by vitrification. The vitrification solution (PVS) contains (w/v) 22% glycerol, 15% ethylene glycol, 15% propylene glycol and 7% DMSO in Murashige-Skoog medium enriched with 0.5M sorbitol. After initial cryoprotection with sorbitol supplemented MS medium containing 12% ethylene glycol, cells or embryos were exposed stepwise to 85% PVS at 0°C. They were loaded into 0.5 ml transparent straws, and were then plunged directly into liquid nitrogen. After rapid warming, PVS was removed and diluted stepwise. The highest survivals of vitrified cells and embryos were about 65 and 50%, respectively. Surviving embryos developed into plantlets.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00270083
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  • 7
    Digitale Medien
    Digitale Medien
    Lung cyclic nucleotides in exercise-trained rats attenuate hypoxic pulmonary vasoconstriction (1995)
    Springer
    Lung 173 (1995), S. 363-372 
    Details
    ISSN: 1432-1750
    Schlagwort(e): Cyclic AMP ; Cyclic GMP ; Exercise ; Exercise training ; Hypoxia ; Hypoxic pulmonary vasoconstriction ; Pulmonary arterial pressure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract We examined the effects of exercise training on pulmonary arterial blood pressure (Ppa) and on adenosine 3′,5′-cyclic monophosphate (cAMP) and guanosine 3′,5′-cyclic monophosphate (GMP) concentrations in lung tissue at rest and during exercise under hypoxic conditions in catheter-implanted rats. Male Wistar rats were divided into an exercise-trained group (ET, n = 32) and nonexercised control group (control, n = 32). ET rats exercised 40 min/day 6 days/week for 6 weeks, at an altitude of 610 m on a treadmill. The mean Ppa levels of the ET were significantly lower than those of controls at rest and during exercise at 610- and 2500-m altitudes. The exercise-induced mean Ppa increase in the ET was less than that in controls at both 610- and 2500-m altitudes. Resting lung cAMP increased more in the ET than in controls at both 610- and 2500-m altitudes. In ET, cGMP was significantly greater at the 2500-m altitude than at the 610-m altitude at rest and just after exercise. Hypoxic exercise in ET was accompanied by a preferential increase in cGMP but not in cAMP. These results suggest that the intracellular augmentation of cAMP and cGMP in ET plays an important role in attenuating hypoxic pulmonary vasoconstriction (HPV) and exercise-induced increases in Ppa.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00172143
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  • 8
    Digitale Medien
    Digitale Medien
    Protection against septic shock in mice with SJC13, an azaindolidine derivative that is a cell adhesion molecule inhibitor (1996)
    Springer
    Inflammation research 45 (1996), S. 448-451 
    Details
    ISSN: 1420-908X
    Schlagwort(e): Septic shock ; Neutrophil ; Cell adhesion molecule inhibitor ; Azaindolidine derivative ; Mice
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract An azaindolidine derivative, SJC13, selectively inhibits expression and mRNA synthesis of E-selectin and vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVEC) stimulated with lipopolysaccharide (LPS). The present experiments were performed to determine the in vivo effects of SJC13 against the lethality of LPS. In a mouse model of septic shock, intravenous administration of SJC13 5 min prior to LPS injection prevented significantly the lethality at doses of 3 mg/kg and 10 mg/kg. The prophylactic effect was dose-dependent. When injected up to 1h after LPS injection, SJC13 inhibited significantly the lethality. Neutrophil emigration into lung tissues during sepsis induced with LPS, as assessed by lung myeloperoxidase (MPO) content and histological examination, was significantly prevented by SJC13 administration. These data demonstrate that SJC13 has therapeutic anti-inflammation potential in vivo.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02252315
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  • 9
    Digitale Medien
    Digitale Medien
    Inhibition of endothelial cell adhesion molecule expression with SJC13, an azaindolidine derivative, in vitro (1996)
    Springer
    Inflammation research 45 (1996), S. 224-229 
    Details
    ISSN: 1420-908X
    Schlagwort(e): E-selectin ; Intercellular adhesion molecule-1 ; Vascular cell adhesion molecule-1 ; Vascular endothelial cells ; Azaindolidine derivative
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Stimulation of cultured human umbilical vein endothelial cells (HUVEC) with lipopolysaccharide (LPS) induces adherences for human promyelocytic cell line HL60. Adherence of HL60 cells to HUVEC stimulated with LPS for 4h was completely inhibited by pretreatment with SJC13, an azaindolidine derivative. The mechanism whereby SJC13 inhibits the adhesiveness of HUVEC was investigated. Pretreatment of SJC13 inhibited LPS-induced expression of E-selectin and vascular cell adhesion molecule-1 (VCAM-1), but not intercellular adhesion molecule-1 (ICAM-1), in HUVEC, determined by flow cytometry and cellular enzyme-linked immunosorbent assay (cell-ELISA). The inhibitory activity was concentration dependent between 62.5 and 1,000 μg/ml. SJC13 also selectively inhibited LPS-induced increases in E-selectin and VACM-1 mRNAs, indicating that the action of SJC13 is to inhibit synthesis of these molecules. These data demonstrate that SJC13 is capable of selectively inhibiting the expression of E-selectin and VCAM-1, but not ICAM-1, in endothelial cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02259607
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  • 10
    Digitale Medien
    Digitale Medien
    Abstracts of the Twenty-second Annual Meeting of the Japanese Society of Biometeorology, Kanazawa, 28–29 October 1983 (1985)
    Springer
    International journal of biometeorology 29 (1985), S. 361-384 
    Details
    ISSN: 1432-1254
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Geographie , Physik
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02189212
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