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  • 1
    Electronic Resource
    Electronic Resource
    Simulation of Hot-Dip Galvanizing for Automotive Applications: Requirements for Modern Steel Product Design (1998)
    s.l. ; Stafa-Zurich, Switzerland
    Materials science forum Vol. 284-286 (June 1998), p. 559-566 
    Details
    ISSN: 1662-9752
    Source: Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://www.tib-hannover.de/fulltexts/2011/0528/02/03/transtech_doi~10.4028%252Fwww.scientific.net%252FMSF.284-286.559.pdf
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  • 2
    Electronic Resource
    Electronic Resource
    Crossreactivity between Dermatophagoides siboney and other house dust mite allergens in sensitized asthmatic patients (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 25 (1995), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background The role of Pyroglyphid mites in IgE-mediated serisitizalion has been well eslahlished. Although mites belonging to the genus Dermatophagoidess dominate in the acarofauna of domestic dust. non-pyroglyphid mites might also be of clinical importance. In Cuba, Dermatophagoides siboney is found in dust coexisting with D. pteronyssiuus and Blomia tropicalis. Dermatophagoides forinae is not found. Storage mites, such as Acorus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae might also be present.Objective The aim of this study was to investigate the allergenic relationships among the mites present in the environment of allergic patients in Cuba.Methods The crossreactivialty between an extract of D. siboney and the above mentioned six mites was studied by specific IgE inhibition, using sera of mite-sensitive patients after incubation with serial dilutions of D. sibaney.Results The inhibitory effect of D. sidiney extract was more pronounced against D. farinae. 91%, than against D. pyteronyssinus, 62%. Specific IgE to B. tropicali, A. siro, L. destructor and T. putrescentiae were inhibited to a much lesser extent, i.e. between 47 and 58%. The correlation between IgE to D. siboney and IgE to D. pteronyssinus and D. farinae was of 0.92 and 0.90. respectively. A weaker correlation was found between D. siboney and L. destructor (0.7), A. siro (0.67), B. tropicalis (0.54) and T. putrescentiae (0.51).Conclusion Thus. D. siboney seems to be most closely related to D. farinae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1995.tb00394.x
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  • 3
    Electronic Resource
    Electronic Resource
    Biologic activity of Dermatophagoides siboney and Blomia tropicalis allergens in exposed and unexposed mite-allergic individuals. Effect of patient selection on the biologic standardization of mite extracts (1999)
    Copenhagen : Munksgaard International Publishers
    Allergy 54 (1999), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: This study aimed to investigate the influence of patient selection criteria, i.e., mite-allergic individuals exposed and not exposed to Dermatophagoides siboney and Blomia tropicalis, on the biologic activity of mite extracts. Determination of the potency of mite extracts in vivo requires selection of patients with a clinical history of mite allergy. In Scandinavia, there are some anamnestic criteria for mite allergy, whereas in the tropics, where patients are continuously exposed to high levels of mites, selection of patients with mite allergy by clinical history is difficult. Methods: A total of 210 Cuban asthmatics with continuous symptoms, and 43 Swedes with a clinical history of mite allergy were investigated. Skin prick tests were performed with D. siboney, D. pteronyssinus, D. farinae, B. tropicalis, Acarus siro, Lepidoglyphus destructor, and Tyrophagus putrescentiae extracts. For analysis of the biologic activity of mite extracts, Cuban patients were divided into four groups:1)all patients skin-test-positive to mites2)patients positive to mites, but not to other inhalant allergens3)patients reacting most to the mite species analyzed4)patients reactive only to mites and reacting most to the mite species analyzed.The biologic potency was calculated according to the Nordic Guidelines. Results: Due to cross-reactivity between mites, Swedish mite-sensitive patients, with a clear clinical history of mite allergy, but not exposed to D. siboney and B. tropicalis, were more skin reactive to these mites than were Cubans. The estimated potency increased gradually to 〉200% in group 4. In group 1 Cubans, the reactivity to all mites but B. tropicalis was lower than that in mite-sensitive Swedes. Conclusions: According to the influence of patient selection criteria on the estimation of the potency of mite extracts, the determination of the biologic activity of allergenic extracts in subjects without a clear-cut clinical history should be replaced by new methods when available.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.1999.00875.x
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  • 4
    Electronic Resource
    Electronic Resource
    Effect of applied benzyladenine on endogenous cytokinin content during the early stages of bud development of kiwifruit (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 95 (1995), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: A short-term study linked directly to our previous long-term experiments was conducted to clarify whether or not benzyladenine (BA) acts directly or through endogenous cytokinins in the development of kiwifruit explants. The uptake and metabolism of BA were characterized in kiwifruit explants after 0.5, 1, 2, 8 and 16 h exposure to 8-[14C]BA on both solidified and liquid Murashige and Skoog media. Endogenous cytokinins were measured by enzyme-linked immunosorbent assay (ELISA) using polyclonal antibodies. The kinetics of BA uptake by explants cultured under these conditions were different, being higher in explants cultured in liquid medium. The glucosylation rate of BA was faster in explants cultured on solid medium. The levels of active forms of BA (BA and [9R]BA) were higher in explants cultured on liquid medium due to its higher uptake and lower glucosylation. The levels of zeatin-type cytokinins were influenced by the culture conditions. A direct correlation was observed between morphogenic response and proportion of active forms of BA, but not with zeatin-type cytokinins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1995.tb00833.x
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  • 5
    Electronic Resource
    Electronic Resource
    Uptake, Distribution, and Metabolism of 1-Naphthaleneacetic Acid and Indole-3-Acetic Acid During Callus Initiation From Actinidia deliciosa Tissues (1999)
    Springer
    Journal of plant growth regulation 18 (1999), S. 81-88 
    Details
    ISSN: 1435-8107
    Keywords: Key Words.Actinidia deliciosa—Auxin—Callus—Indoleacetic acid—Kiwifruit—Naphthaleneacetic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract. 1-Naphthaleneacetic acid (NAA) and 6-benzyladenine (BA) were required for in vitro callus formation at the basal edge of kiwifruit (Actinidia deliciosa [A. Chev] Liang and Ferguson, cv. Hayward) petioles. The uptake, metabolism, and concentration of NAA and indole-3-acetic acid (IAA) content were examined in the explants during the callus initiation period. After 1, 6, 12, 24, 48, and 96 h of culture in the presence of [H3]NAA, petioles were divided into apical, middle, and basal portions and analyzed. Except for a high IAA level measured at 12 h, IAA content decreased in tissues during a culture period of 96 h. NAA uptake was higher in petiolar edges than in the middle portion, and NAA was rapidly conjugated with sugars and aspartic acid inside the tissues. The amide conjugation was triggered in apical and basal portions from 12 h and in the middle part from 48 h, with α-naphthylacetylaspartic acid being the major metabolite. Free-NAA concentration in cultured petioles achieved an equilibrium with the exogenously applied NAA (0.27 μm) from 12 h, and it remained constant thereafter. The relationships between the role attributed to NAA and BA in the initiation and the maintenance of disorganized growth of callus in kiwifruit cultures are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00007053
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  • 6
    Electronic Resource
    Electronic Resource
    Relationship between endogenous auxin and cytokinin levels and morphogenic responses inActinidia deliciosa tissue cultures (1996)
    Springer
    Plant cell reports 16 (1996), S. 58-62 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thein vitro culture ofActinidia deliciosa petioles results in a decline of cytokinin content and an increase of auxin levels. The addition of plant growth regulators (PGRs) to the medium lead to recovery of the initial auxin content, and callus induction occurs at the basal end of the explants. Endogenous auxin/cytokinin ratio was higher at this side than in the apical one, due to unequal distribution of endogenous PGRs in the cultured petioles. Some of the induced calluses showed shoot formation when they were transferred to proliferation medium. Most important differences found in hormonal content between organogenic and non-organogenic callus concerned benzyladenine levels. In this paper the relationships between explant behaviour and their hormonal content is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275450
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  • 7
    Electronic Resource
    Electronic Resource
    Relationship between endogenous auxin and cytokinin levels and morphogenic responses in Actinidia deliciosa tissue cultures (1996)
    Springer
    Plant cell reports 16 (1996), S. 58-62 
    Details
    ISSN: 1432-203X
    Keywords: Abbreviations: BM, basal medium, BA, 6-benzyladenine, CIM, callus induction medium, CPM, callus proliferation medium, (diH)Z, dihydrozeatin, DW, dry weight, ELISA, enzyme linked immunoassay, FW, fresh weight, HPLC, high performance liquid chromatography, IAA, indole-3-acetic acid, iP, N6-(Δ2-isopentenyl)adenine, NAA, 1-naphthaleneacetic acid, PBS, phosphate buffer, PGR, plant growth regulator, (diH)[9R]Z, 9-β-D-ribofuranosyl-(diH), [9R]iP, 9-β-D-ribofuranosyl-iP, [9R]Z, 9-β-D-ribofuranosylzeatin, TBS, trishydroxymethyl-aminomethane buffer, Z, zeatin.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary. The in vitro culture of Actinidia deliciosa petioles results in a decline of cytokinin content and an increase of auxin levels. The addition of plant growth regulators (PGRs) to the medium lead to recovery of the initial auxin content, and callus induction occurs at the basal end of the explants. Endogenous auxin/cytokinin ratio was higher at this side than in the apical one, due to unequal distribution of endogenous PGRs in the cultured petioles. Some of the induced calluses showed shoot formation when they were transferred to proliferation medium. Most important differences found in hormonal content between organogenic and non-organogenic callus concerned benzyladenine levels. In this paper the relationships between explant behaviour and their hormonal content is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050176
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  • 8
    Electronic Resource
    Electronic Resource
    Participation of ACTH1–10 and ACTH4–10 on the melatonin modulation of benzodiazepine receptors in rat cerebral cortex (1995)
    Springer
    Cellular and molecular life sciences 51 (1995), S. 209-212 
    Details
    ISSN: 1420-9071
    Keywords: Melatonin ; hypophysectomy ; ACTH-derived peptides ; benzodiazepine receptor ; brain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In this study, we examined the effect of intracerebroventricular (i.c.v) injection of melatonin and/or ACTH1–10 and ACTH4–10 on [3H]flunitrazepam binding sites in the cerebral cortex of hypophysectomized rats. Hypophysectomy increased the Bmax (maximum number of binding sites) of benzodiazepine (BNZ) receptors for at least 7 days after surgery, without changing KD (dissociation constant). The i.c.v. injection of melatonin to hypophysectomized rats significantly increased Bmax, whereas the same doses of melatonin were ineffective in sham-operated animals. In both cases, KD values were unchanged. The i.c.v injection of ACTH1–10 to hypophysectomized animals significantly increased Bmax, an effect that was enhanced by simultaneous i.c.v. injection of ACTH1–10+melatonin, reaching higher values of Bmax than the i.c.v. injection of these hormones individually. No significant changes in KD values were found after ACTH1–10 and/or melatonin administration. However, the i.c.v. injection of ACTH4–10 to hypophysectomized rats did not change Bmax, although it significantly increased KD values, indicating a decrease in the BNZ binding affinity. Melatonin injection counteracted this effect of ACTH4–10, returning KD to the control value. Moreover, although the lower dose of i.c.v. melatonin used, 10 ng, was unable to modify Bmax of BNZ binding in the ACTH4–10-injected group, the higher dose, 20 ng, significantly increased Bmax. The results suggest that these ACTH-derived peptides can modulate the effect of melatonin on brain benzodiazepine receptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01931097
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  • 9
    Electronic Resource
    Electronic Resource
    Long-term changes in glial fibrillary acidic protein and glutamine synthetase immunoreactivities in the supraoptic nucleus of portacaval shunted rats (1996)
    Springer
    Metabolic brain disease 11 (1996), S. 369-379 
    Details
    ISSN: 1573-7365
    Keywords: Astrocyte ; GFAP ; GS ; glutamate ; portacaval shunt
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The present study was undertaken to ascertain whether, and to what extent, glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS) expressions in the supraoptic nucleus (SON) could be modulated after one month and six months of portacaval shunting (PCS) in rats. GFAP and GS immunoreactivities were significantly higher in PCS rats than in control rats at one and six months. The increased GFAP and GS immunoreactivities observed in the SON astrocytes were directly related to the duration of PCS. In PCS rats, the number and length of both GFAP and GS immunopositive astroglial processes increased not only in the hypothalamic nucleus but in the perinuclear zone, where glutamatergic pathways have been described, whereas GFAP and GS expressions decreased in the ventral glial lamina. Since GS is one of the glutamate metabolizing enzymes and the SON is one of the areas of glutamatergic activity, our results show that astrocytes respond differentially to glutamate toxicity. This suggests that overexpression of GFAP and GS immunoreactivities could be associated with glutamatergic neurotransmission disorders.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02029497
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  • 10
    Electronic Resource
    Electronic Resource
    Maternal pineal gland participates in prepubertal rats' ovarian oocyte development (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 243 (1995), S. 461-465 
    Details
    ISSN: 0003-276X
    Keywords: Maternal pineal gland ; Prepubertal phase ; Oocyte ; Nucleus ; Nucleole ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Background: Sexual maturation is a very complex phenomenom that it is mediated by the ontogeny of the hypothalamus-pituitarygonadal axis during intrauterine life. The maternal pineal gland can affect fetal development because the main pineal hormone, melatonin, crosses the placental barrier. We found that melatonin treatment during gestation in the rat produced delayed sexual maturation of the female offspring. The present work was undertaken to study the maturational stage of oocytes of prepubertal female rats when their mothers were either pinealectomized (PIN-X) or treated with melatonin (MEL) during pregnancy.Methods: Three groups of female Wistar rats were used: control, PIN-X, and those treated (250 μg/100 g body weight) with melatonin throughout pregnancy. Ovaries of 25-30- and 34-day-old female offspring were studied during the prepubertal phase. Morphometric studies of semithin sections (1 μm) of the ovaries were performed. Oocyte, nuclear, and nucleolar volumes were calculated by a computer-assisted program (M.I.P.) in an image analyzer Kontron. Regularity of the structures was determined by the frequency distributions of circular and regular form factors.Results: Cytometric study of oocyte structure showed a frequency distribution of regular and circular form factors, with a high degree of regularity very close to unit. Cellular and nuclear volumes of follicular oocytes showed a transitory increase at 30 days of age in control rats. In the offspring of MEL-treated mother rats, a pattern of oocyte development showed significantly lower nuclear and nucleolar volumes at 30 days of age than at the other time points and significantly lower cellular volume at 34 days of age than at 25 days of age. In the offspring of PIN-X mother rats, no significant differences in oocyte cellular volumes were observed throughout prepubertal development, but we observed a significantly higher nuclear volume at 25 days of age and a significantly lower nucleolar volume at 30 days of age.Conclusions: These findings show that the maternal pineal gland participates in cellular and nuclear volumes of prepubertal oocyte development. Melatonin treatment during pregnancy resulted in a redirected postnatal oocyte development. © 1995 Wiley-Liss, Inc.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092430408
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