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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Development genes and evolution 206 (1996), S. 64-71 
    ISSN: 1432-041X
    Keywords: Key words Annexin V ; Spermatogenesis ; Meiosis ; Cynops pyrrhogaster ; Differential screening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In order to isolate genes whose expression is up-regulated after the initiation of meiosis, we screened a cDNA expression library of newt testes with antiserum against homogenates of testes derived from the spermatogonial and spermatocyte stages. We report the isolation of spermatocyte-specific cDNA clones encoding a newt homologue of the calcium-dependent phospholipid-binding protein, annexin V. Northern blot analysis showed that newt annexin V mRNA was 1.7 kb in length and was expressed strongly in testes, but weakly in other organs. In situ hybridization revealed that the expression of newt annexin mRNA was barely observed in spermatogonia, but increased significantly in leptotene-zygotene primary spermatocytes and reached a maximum level in pachytene spermatocytes and round spermatids. The newt annexin V cDNA predicted a 323-amino acid protein and had a 68% homology to human annexin V. The predicted amino acid sequence contained a conserved 4-fold internal repeat of approximately 70 residues like other annexin proteins. Immunoblot analysis using the monoclonal antibody against newt annexin V showed that the protein was expressed scarcely in spermatogonia but was abundantly expressed in stages from primary spermatocytes to spermatids; this pattern was consistent to that of the mRNA. Immunohistochemical analysis revealed that newt annexin V was localized in the cytoplasm of the spermatogenic cells, but not in somatic cells such as Sertoli cells or pericystic cells. These results indicate that the expression of newt annexin V is up-regulated in the spermatogenic cells after the initiation of meiosis and suggest that newt annexin V plays an important role in spermatogenesis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0992-7689
    Keywords: Ionosphere (Auroral, ionosphere) ; Magnetospheric Physics (Magnetotail; Storms and substorms)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract During the 6th August 1995, the CUTLASS Finland HF radar ran in a high time resolution mode, allowing measurements of line-of-sight convection velocities along a single beam with a temporal resolution of 14 s. Data from such scans, during the substorm expansion phase, revealed pulses of equatorward flow exceeding ∼600 m s−1 with a duration of ∼5 min and a repetition period of ∼8 min. Each pulse of enhanced equatorward flow was preceded by an interval of suppressed flow and enhanced ionospheric Hall conductance. These transient features, which propagate eastwards away from local midnight, have been interpreted as ionospheric current vortices associated with fieldaligned current pairs. The present study reveals that these ionospheric convection features appear to have an accompanying signature in the magnetosphere, comprising a dawnward perturbation and dipolarisation of the magnetic field and dawnward plasma flow, measured in the geomagnetic tail by the Geotail spacecraft, located at L = 10 and some four hours to the east, in the postmidnight sector. These signatures are suggested to be the consequence of the observation of the same field aligned currents in the magnetosphere. Their possible relationship with bursty Earthward plasma flow and magnetotail reconnection is discussed.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1420-9071
    Keywords: Estrogen ; receptor ; tumor ; submandibular gland ; 9,10-dimethyl-1,2-benzanthracene ; female rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We have demonstrated the presence of estrogen receptor mRNA and the mature protein in the cytoplasm and nucleus, respectively, of a 9,10-dimethyl-1,2-benzathracene-induced submandibular gland tumor in female rats. We have previously shown that progesterone receptors are also present in human salivary gland tumors. These results suggest that endocrine therapy may be effective in treatment of submandibular gland tumors.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-0568
    Keywords: Key words Cellular cementum ; Intrinsic fiber ; Cementoblast ; Human tooth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  The formation of an alternate lamellar pattern in the advanced stage of cellular cementogenesis in human molars was examined by light and electron microscopy. In longitudinal ultrathin sections, longitudinally oriented intrinsic fibril bundles appeared in close and parallel association with slender processes of cementoblasts on the cementum. Where transversely oriented intrinsic fibril bundles appeared, cementoblasts formed indentations to enclose the fibril bundles. Cytoplasmic fragments were also enclosed in the indentations. Scanning electron microscopy indicated that cementoblasts have developed two types of processes on their cementum-facing side – ridge- and finger-like. The cementoblasts formed groove-like compartments by ridge-like processes in cooperation with other cementoblasts. The compartments formed groups, and in each group the compartments were arranged in the same direction. The finger-like processes were arranged in parallel with the ridge-like processes in the compartments. These observations suggest that: (1) slender processes and cytoplasmic fragments are longitudinally and transversely cut finger-like processes, respectively; (2) the cellular indentations are transversely cut groove-like compartments; (3) the cementoblasts regulate the intrinsic fiber arrangement by the two types of processes; (4) the cementoblasts move the two types of processes synchronously and periodically to cause an alternate change in the intrinsic fiber arrangement. This dynamic sequence results in the alternate lamellar pattern of cellular cementum.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-0509
    Keywords: Key words: Pancreatitis—Pseudocyst—Endoscopic retrograde pancreaticocholangiography—Doppler sonography.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pseudocyst formation is a well-known complication of acute or chronic pancreatitis. We report a case in which pseudocyst ruptured into the splenic and portal veins.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Environmental geology 28 (1996), S. 88-98 
    ISSN: 1432-0495
    Keywords: Key words River sand ; Geochemical elements ; X-ray fluorescent spectroscopy ; Correlation number ; Ratio matching ; Correlation matrix ; Geological characteristics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract  The analysis of sand samples by X-ray fluorescent spectroscopy (XRF) gives the ratio of the geochemical elements to construct the sand samples. The application of the ratio matching to sand samples represents the correlation number between two sand samples with respect to geochemical elements. When the correlation number between two sand samples is low, the two samples are not geochemically similar each other. This denotes that the exchange of sand between two sampling points is scarce or the two samples are independent. When the correlation number between two sand samples is high, the two samples are geochemically similar, signifying that the exchange of sand between two sampling points is frequent or there is sand movement between two sampling points. If there exists prominent sand movement in the study area, the correlation number is almost 1 and kilo count number per second of each geochemical element per weight decreases along the flow direction. The decrease is caused by the reduction of sizes and the adherence of dirt on the surface of sand particles. Since the flow direction in rivers is usually the same as the direction of sand movement, it can be verified. This study obtains satisfactory results applying the method of prediction in sand movement to sediments in rivers.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Environmental geology 25 (1995), S. 100-108 
    ISSN: 1432-0495
    Keywords: Sand movement ; Geochemistry ; Fluorescent spectroscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract The analysis of sand samples by x-ray fluorescent spectroscopy (XRF) gives the ratio of the geochemical elements to construct the sand samples. The x-ray analysis therefore shows the geochemical characteristics of sand in the sampled area. In this study, sand is sampled on coasts and rivers of the Noto Peninsula to determine the geochemical elements and to show the geological characteristics that occur, especially iron and calcium. The experiments show the effect of rivers and Kotogahama beach on iron and calcium, respectively. Applying the method of ratio matching to the measured data of the geochemical elements, the direction of movement of sand on the coast is determined by considering the correlation matrix and the ratio of geochemical elements between sand samples at two locations. The predominant longshore direction movement of sand offshore in the study area is from south to north. Sand in rivers is not directly transported to adjacent beaches; however, offshore sand is transported to beaches. The estimated direction of movement of sand longshore near coastal structures agrees with observations. The proposed method to predict the direction of movement of sand gives the correct one in comparison with the observed data in rivers.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Microsystem technologies 5 (1999), S. 169-172 
    ISSN: 1432-1858
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Technology
    Notes: Abstract  A diffusion layer that is likely to be formed at the interfaces of the multi-layered thin film would affect its overall mechanical properties; the thinner the thin film, the more significant would be the effect. We measure the distribution of atoms and estimate the thickness of the diffusion layer at the vicinity of the interfaces among thin films of Al and SiO2 and silicon wafer with the aid of Auger electron spectroscopy (AES). The effect of heat treatment after fabrication of the thin films on the diffusion is also investigated.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 0992-7689
    Keywords: Magnetospheric configuration and dynamics ; Magnetotail
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Geotail energetic particle, magnetic field data and plasma observations (EPIC, MGF and CPI experiments) have been examined for a number of energetic particle bursts in the distant tail (120Re 〈 |XGSM| 〈 130 Re), associated with moving magnetic field structures, following substorm onsets. The features obtained from this data analysis are consistent with the distant magnetotail dynamics determined first by ISEE3 observations and explained in terms of the neutral line model. At the onset of the bursts, before plasma sheet entrance, energetic electrons appear as a field-aligned beam flowing in the tailward direction, followed by anisotro-pic ions. Within the flux rope region, suprathermal ions exhibit a convective anisotropy, which allows determination of the plasma flow velocity, assuming that the anisotropy arises from the Compton-Getting effect. The velocities thus determined in the plasma sheet are estimated to be 200–650 km/s, and compare favourably with the velocities derived from the CPI electron and proton experiment. The estimated length of magnetic field structures varies between 28 and 56 Re and depends on the strength of the westward electrojet intensification. Finally, the three structures reported here show clear magnetic field signatures of flux rope topology. The existence of a strong magnetic field aligned approximately along the Y-axis and centred on the north-to-south excursion of the field, and the bipolar signature in both By and/or Bz components, is consistent with the existence of closed field lines extending from Earth and wrapping around the core of the flux rope structure.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 0992-7689
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract We have analyzed the onsets of energetic particle bursts detected by the ICS and STICS sensors of the EPIC instrument on board the GEOTAIL spacecraft in the deep magnetotail (i.e., at distances greater than 180 RK). Such bursts are commonly observed at the plasma-sheet boundary layer (PSBL) and are highly collimated along the magnetic field. The bursts display a normal velocity dispersion (i.e., the higher-speed particles are seen first, while the progressively lower speed particles are seen later) when observed upon entry of the spacecraft from the magnetotail lobes into the plasma sheet. Upon exit from the plasma sheet a reverse velocity dispersion is observed (i.e., lower-speed particles disappear first and higher-speed particles disappear last). Three major findings are as follows. First, the tailward-jetting energetic particle populations of the distant-tail plasma sheet display an energy layering: the energetic electrons stream along open PSBL field lines with peak fluxes at the lobes. Energetic protons occupy the next layer, and as the spacecraft moves towards the neutral sheet progressively decreasing energies are encountered systematically. These plasma-sheet layers display spatial symmetry, with the plane of symmetry the neutral sheet. Second, if we consider the same energy level of energetic particles, then the H ‘ layer is confined within that of the energetic electron, the He++ layer is confined within that of the proton, and the oxygen layer is confined within the alpha particle layer. Third, whenever the energetic electrons show higher fluxes inside the plasma sheet as compared to those at the boundary layer, their angular distribution is isotropic irrespective of the Earthward or tailward character of fluxes, suggesting a closed field line topology.
    Type of Medium: Electronic Resource
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