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  • 1
    Electronic Resource
    Electronic Resource
    Optimal design of the tubular microporous membrane aerator for shear-sensitive cell cultures (1992)
    s.l. : American Chemical Society
    Biotechnology progress 8 (1992), S. 19-24 
    Details
    ISSN: 1520-6033
    Source: ACS Legacy Archives
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bp00013a004
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  • 2
    Electronic Resource
    Electronic Resource
    Production of rosmarinic acid in high density perfusion cultures ofAnchusa officinalis using a high sugar medium (1990)
    Springer
    Biotechnology letters 12 (1990), S. 793-798 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The most direct approach to enhancing the volumetric yield of secondary metabolites in plant tissue cultures is to operate the culture under high cell density. In this study, a cell suspension ofAnchusa officinalis was cultivated using a semi-continuous perfusion technique, i.e. batch cultivation with intermittent medium exchange. Using a perfusion medium containing sucrose concentration which was two times that in the normal growth medium, the final cell density and the final product concentration were increased by more than 2-fold compared with a batch culture without medium exchange. The high cell density obtained from the semi-continuous perfusion culture can be explained by the prevention of nutrient depletion, removal of toxic by-products, as well as the control of cell size by virtue of the high sugar medium osmolarity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01022597
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  • 3
    Electronic Resource
    Electronic Resource
    Production of rosmarinic acid from perfusion culture ofAnchusa officinalis in a membrane-aerated bioreactor (1991)
    Springer
    Biotechnology letters 13 (1991), S. 889-892 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary In this study, a perfusion fermentation ofAnchusa officinalis was carried out in a stirred tank bioreactor integrated with an internal cross-flow filter. Bubble-free aeration via microporous membrane fibers was used to provide oxygen. A two-stage culture was successfully conducted in this reactor without filter fouling. In a 17 day fermentation, a cell density of 26 g dw/I and a rosmarinic acid productivity of 94 mg/l day were achieved. This productivity is three times that obtained in a batch culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01022093
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Location of monitorial fluorophores inCandida utilis (1992)
    Springer
    Biotechnology techniques 6 (1992), S. 227-232 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The fluorescent spectra of whole broth, cell-free broth and resuspended cells were compared during the fermentation ofCandida utilis growing in ethanol for the purposes of identifying the location of monitorial fluorophores in cellular systems. Four cellular fluorophores, tryptophan, pyridoxine, NADH and riboflavin were examined. The results indicated that the fluorescence signal of tryptophan and NADH came mainly from intracellular fluorophores, and the fluorescence signal of pyridoxine and riboflavin mainly came from extracellular fluorophores. The contributions of intracellular and extracellular fluorophores to culture fluorescence signals were found to change during the fermentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02439349
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  • 5
    Electronic Resource
    Electronic Resource
    A study of nicotine release from tobacco hairy roots by transient technique (1993)
    Springer
    Biotechnology techniques 7 (1993), S. 557-562 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A material balancing method for determining the productivity of nicotine-releasing Nicotiana tabacum hairy roots is presented. Diffusive nicotine release from the intracellular environment of N. tabacum to its medium is based upon an equilibrium partitioning process. By removing nicotine from a culture's medium, subsequent transient release from the tissue is induced, as is the heightened production of nicotine. Under normal conditions, sixteen hours is required for releasing tissue to re-establish equilibrium with the medium. 100 ppm of the surfactant Triton X-100 was used to enhance the rate of nicotine release and to reduce the return to equilibrium to four hours without toxic side-effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00156328
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  • 6
    Electronic Resource
    Electronic Resource
    Use of fluorometry for monitoring and control of a bioreactor (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 37 (1991), S. 1043-1049 
    Details
    ISSN: 0006-3592
    Keywords: Multiple excitation fluorometric system (MEFS) ; NADH probe ; culture fluorescence ; inner filter effects ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new fluorescent bioreactor monitoring probe - multiple excitation fluorometric system (MEFS) - has been developed. This probe was compared to the commercially available BioChem Technology FluroMeasure system (NADH probe). In this task the fluorescence behavior of three model fermentation systems, ethanol fermentation by Candida utilis, phenol fermentation by Pseudomonas putida, and glucose fermentation by Saccharomyces cerevisiae, were examined. The results indicated that the fluorescence intensity and behavior of various cellular fluorophors vary significantly between the different fermentation systems. Monitoring a fermentation process using only NAD(P)H fluorescence provided limited information. The NAD(P)H fluorescence was found not to be the best fluorescence signal for monitoring cell concentrations. The best way of monitoring a bioreactor by fluorometry may be to monitor several fluorophors in the whole culture broth simultaneously and to relate these fluorescence signals to various biological parameters.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260371109
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  • 7
    Electronic Resource
    Electronic Resource
    Production of expansin from light/dark growing Trichosanthes kirilowii var. Japonicum root cultures (1994)
    Springer
    Biotechnology letters 16 (1994), S. 955-958 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Actively growing Trichosanthes kirilowii var. Japonicum root culture grown under light produces two proteins that are similar to the S1 and S2 proteins (expansin) extracted from Cucumis sativus cv Burpee Pickler stems. These proteins play a role in the expansion (swelling) of cell walls. Since the root cultures can be easily handled and scaled up, root cultures may be an alternative source for the production of expansin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00128632
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  • 8
    Electronic Resource
    Electronic Resource
    Screening microorganisms for utilization of furfural and possible intermediates in its degradative pathway (1994)
    Springer
    Biotechnology letters 16 (1994), S. 977-982 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A variety of microorganisms were screened for their ability to utilize furfural and possible intermediates in its degradative pathway. Compounds tested included furfural, furfuryl alcohol, 2-furoic acid, tetrahydrofurfuryl alcohol, di- and tetra-hydrofurans, 1,5-pentanediol and 1,4-butanediol. The objective of the research was to determine whether microbial strains might be found that degraded furfuryl via diols. If so, then it might be possible to block the metabolic pathway at the appropriate point allowing accumulation of diol(s). A promising bacterial strain was identified that could utilize furfural, some of its derivatives and diols, which is a candidate for further studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00128637
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  • 9
    Electronic Resource
    Electronic Resource
    Stimulation of solavetivone synthesis in free and immobilized cells of Hyoscyamus muticus by Rhizoctonia solani fungal components (1993)
    Springer
    Biotechnology letters 15 (1993), S. 307-310 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Three elicitor fractions including spent culture medium, hydrolyzed mycelial pellet, and hydrolyzed mycelial biomass supernatant were tested for their ability to induce sesquiterpene formation in freely suspended and immobilized cells. Immobilization enhanced the effectiveness of the hydrolysate elicitors, but not that derived from spent culture medium. When effectiveness of the elicitors is compared based on product yield, spent culture medium is shown to be far more effective than hydrolysates as an elicitor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00128324
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  • 10
    Electronic Resource
    Electronic Resource
    Production of solavetivone by immobilized cells of Hyoscyamus muticus (1993)
    Springer
    Biotechnology letters 15 (1993), S. 301-306 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The secretion of solavetivone, a phytoalexin, by the cells of Hyoscyamus muticus in response to fungal elicitation has been enhanced by gel entrapment in calcium alginate. The immobilized cells produced 53% higher product and exhibited sustained biosynthetic activity in repeated batch cycles when compared with suspended cells. Providing a non-elicited media exchange and a sufficient interval of time between repeated infection (elicitation) gave greater productivity. Apparently the cells need a period to recover from the infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00128323
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