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  • 1
    Electronic Resource
    Electronic Resource
    Growth hormone stimulates proliferation and differentiation of normal human osteoblast-like cells in vitro (1993)
    Springer
    Calcified tissue international 52 (1993), S. 222-226 
    Details
    ISSN: 1432-0827
    Keywords: Growth hormone ; Osteoblast ; Growth factors ; Osteoporosis ; Bone cell cultures ; Bone formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary In this study we investigated the direct, shortterm effects of human growth hormone (hGH) on the biology of normal adult human osteoblast-like (hOB) cells cultured from trabecular bone explants. In Subconfluent cultures, hGH stimulated hOB proliferation in a dose-dependent fashion (P〈0.001, n=15) with half-maximal effects at a concentration of 10 ng/ml. These mitogenic effects were detectable within 24 hours as shown by bromodeoxyuridine labeling. In confluent cultures containing mainly quiescent cells, hGH increased levels of alkaline phosphatase (P〈0.05, n=10) and to a lesser degree levels of procollagen type I carboxyterminal propeptide (PICP) (P=0.07, n=9). Effects on osteocalcin (bone GLa protein, BGP) levels were highly variable among different cell strains and only 7 of 10 cell strains showed a stimulatory response (P=0.16). We also studied the effects of hGH on osteoblastic production of insulin-like growth factor I (IGF-I) and IGF-II as well as the production of GH-dependent, insulin-like growth factor binding protein 3 (IGFBP-3). Under basal conditions, human osteoblasts produced IGF-II and IGFBP-3 in the conditioned medium. When stimulated with hGH, minor insignificant increase in both IGF-II and IGFBP-3 (125% and 126% of control, respectively) were detectable. No IGF-I was detectable in the conditioned medium under basal conditions or after stimulation with hGH. In conclusion, the results obtained in this study suggest that GH exerts direct anabolic effects on human osteoblasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00298723
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  • 2
    Electronic Resource
    Electronic Resource
    Basal and insulin stimulated substrate metabolism in tumour induced hypoglycaemia; evidence for increased muscle glucose uptake (1991)
    Springer
    Diabetologia 34 (1991), S. 17-20 
    Details
    ISSN: 1432-0428
    Keywords: Glucose turnover ; forearm technique ; intermediary metabolites ; euglycaemic and hypoglycaemic glucose clamp ; indirect calorimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary While it has very recently been reported that tumour induced hypoglycaemia is characterised by elevated production of insulin-like growth factor 2, the tissues responsible for induction of hypoglycaemia are largely unknown. We have investigated a patient with a large retroperitoneal mass and spontaneous hypoglycaemia. When compared to a reference population the patient displayed: (1) An increased glucose disposal rate and a five-fold elevation of forearm glucose uptake. (2) A decreased endogenous glucose production rate. (3) Decreased circulating levels of lipid intermediates. (4) Increased glucose oxidation and decreased lipid oxidation. (5) Low circulating levels of insulin-like growth factor 2 and insulin-like growth factor-binding protein-3 and normal levels of insulin-like growth factor 1. (6) Normal insulin sensitivity (euglycaemic glucose clamp). Blood concentrations of insulin, C-peptide, proinsulin, glucagon, growth hormone and catecholamines were within normal range, but the growth hormone response to hypoglycaemia was blunted. The data suggest that the mechanisms behind tumour induced hypoglycaemia are of systemic nature and that the tissue most prominently affected is striated muscle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00404019
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  • 3
    Electronic Resource
    Electronic Resource
    Accumulation of scopoletin is associated with the high disease resistance of the hybrid Nicotiana glutinosa x Nicotiana debneyi (1993)
    Springer
    Planta 191 (1993), S. 200-206 
    Details
    ISSN: 1432-2048
    Keywords: Disease resistance ; Nicotiana ; Scopoletin ; Scopolin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The high disease resistance of the amphidiploid hybrid of Nicotiana glutinosa x Nicotiana debneyi is associated with high constitutive levels of two phenolic compounds as analysed by high-performance liquid chromatography. The structures of these two compounds were elucidated by means of gas chromatography-tandem mass spectrometry, fluorescence- and light-spectrophotometry to be those of scopolin and scopoletin. They reached levels of 4 nmol·(g FW)−1 and 35 nmol·(g FW)−1, respectively, in leaf tissues of the hybrid, about 10–50 times the amount found in the parental species. Scopoletin showed a direct antimicrobial activity against Cercospora nicotianae, Phytophthora parasitica var. nicotianae, Pseudomonas syringae pvs. tabaci and syringae and tobacco mosaic virus when added to synthetic growth media, mixed with the inoculum or sprayed onto tobacco plants prior to inoculation. We postulate that the high amount of toxic phenolics in the leaves of the hybrid N. glutinosa x N. debneyi contributes to its high disease resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00199750
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  • 4
    Electronic Resource
    Electronic Resource
    Placebo-controlled, double-blind, cross-over trial of growth hormone treatment in prepubertal children with chronic renal failure (1992)
    Springer
    Pediatric nephrology 6 (1992), S. 272-272 
    Details
    ISSN: 1432-198X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00878370
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  • 5
    Electronic Resource
    Electronic Resource
    Growth hormone treatment in children with preterminal chronic renal failure: no adverse effect on glomerular filtration rate (1993)
    Springer
    Pediatric nephrology 7 (1993), S. 391-391 
    Details
    ISSN: 1432-198X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00857547
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  • 6
    Electronic Resource
    Electronic Resource
    Growth hormone treatment in children with preterminal chronic renal failure: No adverse effect on glomerular filtration rate (1992)
    Springer
    European journal of pediatrics 151 (1992), S. 601-607 
    Details
    ISSN: 1432-1076
    Keywords: Chronic renal failure ; Recombinant human growth hormone treatment ; Insulin-like growth factors ; Insulin-like growth factor binding proteins ; Progression of renal disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Impaired growth and stunting remains a major therapeutic problem in children with chronic renal failure (CRF). Recombinant human growth hormone (rhGH) treatment may be beneficial, but concern has been raised about possible side-effects, i.e. deterioration of renal function and glucose intolerance. We have treated 10 prepubertal children with CRF (median age 7.5 [1.7–10.0] years) with 4 IU rhGH/m2 per day s.c. over a period of 1 year. Height velocity increased significantly (P〈0.03) from basal 4.6 (2.0–14.0) cm/year to 9.7 (6.8–17.6) cm/year. Height velocity SDS for chronological age and for bone age increased in all children from basal median −2.3 to +3.8 (P〈0.005). Median glomerular filtration rate (GFR) measured by single injection inulin clearance at onset was 18 (11–66) ml/min per 1.73 m2 and did not change significantly during the treatment year. The loss of GFR as estimated by creatinine clearance was similar during the treatment year (median loss 1.3 ml/min per 1.73 m2) compared to the year before treatment (median loss 3.7 ml/min per 1.73 m2). Serum glucose levels during an oral glucose tolerance test did not change, but fasting as well as stimulated insulin levels increased significantly with time during the study period. It is concluded that the rhGH regimen employed was remarkably effective in improving growth velocity in children with CRF without adversely affecting GFR. Glucose homeostasis remained stable, but at the expense of increased serum insulin levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01957731
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  • 7
    Electronic Resource
    Electronic Resource
    Zur Morphologie der Lungentuberkulose beim Vergleich von Bronchogramm und Angiogramm (1953)
    Springer
    Lung 109 (1953), S. 528-540 
    Details
    ISSN: 1432-1750
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Im Hinblick auf die zunehmende Bedeutung der Segmentresektion für die operative Behandlung der Lungentuberkulose ist die präoperative Analyse des einzelnen Lungensegments hinsichtlich seiner funktionellen und morphologischen Situation besonders dringlich. Aus einem Material von 160 selektiven Angiographien der Lungengefäße und 160 Bronchographien bei Lungentuberkulose wird an 5 Beispielen aufgezeigt, daß sich das Bronchogramm mit seinen vorwiegend morphologischen Befunden durch das Angiogramm mit seinen funktionellen Kriterien im Rahmen der Segmentanalyse zu einer funktionellen Morphologie ausweiten läßt. Die wesentlichen Merkmale des pathologischen Angiogramms sind Engerstellung und Streckung der Gefäße mit entsprechender Veränderung der Verzweigungswinkel, Herabsetzung der Zirkulationsgeschwindigkeit des Kontrastmittels, Gefäßabbrüche, Rarefizierung im Bereich des peripheren Gefäßnetzes und Änderungen der Gefäßtopographie. Durch die Abhängigkeit der Zirkulation von der Ventilation sind Schlüsse auf das funktionelle Spiel der Alveolen möglich, wenn die Durchblutung durch die selektive Angiographie sichtbar gemacht wird. An Hand der ausgewählten Beispiele, deren bronchographische und angiographische Befunde außerdem operativ nachkontrolliert werden konnten, werden die Ergebnisse der beiden Untersuchungsverfahren miteinander verglichen und die Möglichkeit ihrer Synthese zu einer funktionellen Morphologie für die Segmentanalyse aufgezeigt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02146052
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  • 8
    Electronic Resource
    Electronic Resource
    Bestimmung des Phosphors in vanadinhaltigem Material (1923)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 63 (1923), S. 124-130 
    Details
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01380404
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  • 9
    Electronic Resource
    Electronic Resource
    Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6-phosphate (IGF-II/M6P) receptors and synthesize IGF-II: Autocrine growth stimulation by IGF-II via the IGF-I receptor (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 159 (1994), S. 531-541 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Recently, insulin-like growth factor-I and -II (IGF-I and -II) have been implicated in the growth promotion of tumors in vivo and tumor cells in vitro. We have studied the human osteosarcoma cell line U-2 OS in order (1) to gain more insight into the growth promoting actions of the IGFs and (2) to establish an in vitro tissue culture model of IGF action in human tumor cells. Specific binding of 125I-IGF-I and 125I-IGF-II to IGF-I receptors and IGF-II/mannose-6-phosphate (M6P) receptors on U-2 OS cells was demonstrated in competitive binding experiments and in affinity crosslinking experiments. Western blotting of cell extracts confirmed the expression of the IGF-II/M6P receptor. In addition, in Northern blotting experiments using total RNA from U-2 OS cells IGF-I receptor RNA of 11 kb and IGF-II/M6P receptor RNA of approximately 9 kb were detected. Solution hybridization experiments confirmed the presence of IGF-I receptor and IGF-II/M6P receptor RNA. In a subset of experiments DNA synthesis was measured as 3H-thymidine uptake into cellular DNA of U-2 OS cells. Normal rat serum stimulated DNA synthesis maximally. IGF-I-deficient serum from hypophysectomized rats as well as IGF-I or IGF-II without serum were approximately twofold and tenfold, respectively, less potent than serum in stimulating 3H-thymidine uptake. The concentrations of IGF-I and IGF-II needed for half maximal stimulation of DNA synthesis corresponded well with the respective concentrations required for half maximal inhibition of 125I-IGF-I binding to U-2 OS cells. The anti-IGF-I receptor antibody alphaIR3 blocked the IGF-I and IGF-II stimulated increase of 3H-thymidine uptake. In addition, basal DNA synthesis was partially inhibited by the anti-IGF-I receptor antibody. These data suggest that U-2 OS cells synthesize and secrete IGF-like peptides. Northern blotting experiments confirmed that U-2 OS cells express an IGF-II RNA species of 5.3 kb but no IGF-I transcripts. In a series of RNase protection assays, protected RNA fragments were detected with an IGF-II riboprobe. © 1994 wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041590317
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