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  • 1
    Electronic Resource
    Electronic Resource
    Acetylcholine-induced change in intracellular Cl− activity of the mouse lacrimal acinar cells (1985)
    Springer
    Pflügers Archiv 405 (1985), S. 108-111 
    Details
    ISSN: 1432-2013
    Keywords: Lacrimal gland ; Cl− activity ; Acetylcholine ; Cl− permeability ; Ca2+ ionophore
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Using double-barreled Cl−-sensitive microelectrodes, intracellular Cl− activity (A Cl i ) in the mouse lacrimal acinar cells in vitro was determined in both resting and secretory phases. In the resting stateA Cl i was 31 mmol/l which was 1.4 times higher than that predicted for the passive distribution according to the membrane potential (V m) of −41 mV. Addition of acetylcholine (ACh, 1μM) hyperpolarizedV m to −63 mV and decreasedA Cl i to 20 mmol/l which was still twice the equilibrium activity. A-23178 produced similar changes inV m andA Cl i to those induced by ACh. It was concluded that Cl− was actively accumulated in the acinar cells and, in the secretory phase, Cl− efflux was enhanced by the increased driving force and Ca2+-mediated increase in the Cl− permeability across the cell membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00584530
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  • 2
    Electronic Resource
    Electronic Resource
    Microfluorimetric imaging study of the mechanism of activation of the Na+/H+ antiport by muscarinic agonist in rat mandibular acinar cells (1991)
    Springer
    Pflügers Archiv 419 (1991), S. 338-348 
    Details
    ISSN: 1432-2013
    Keywords: Na+/H+ exchange ; Intracellular pH regulation ; Salivary gland ; Protein kinase C ; Intracellular Ca2+ ; Acetylcholine ; Ionomycin ; Phorbol esters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The mechanism of regulation of intracellular pH (pHi) in dispersed acini from the rat mandibular salivary gland has been studied with a microfluorimetric imaging method and the pH probe 2′,7′-bis(2-carboxyethyl)-5(and −6)-carboxyfluorescein. The pHi in the TRIS/HEPES-buffered standard solution was 7.29±0.01. Addition of 1 μmol/l acetylcholine (ACh) or ionomycin caused a sustained increase in the pHi. These agents decreased pHi in the absence of external Na+ or in the presence of amiloride. The rate of pHi recovery from an acid load after NH 4 + prepulse was a linear function of pHi and increased as pHi became more acidic. Addition of ACh shifted the relationship towards a more alkaline pHi range. The increase in pHi induced by ACh or ionomycin was not inhibited by the protein kinase C inhibitors staurosporine (10 nM) and 1-(5-isoquinolinesulfonyl)-1-methylpiperazine (50 μmol/l). Addition of 0.1–1 μmol/l phorbol 12-myristate 13-acetate (TPA) had little effect on pHi within 10 min; however, exposure to TPA for 120 min resulted in a significant rise in pHi. In Ca2+-free solution with 50 μmol/l 8-(diethylamino)-octyl-3,4,5-trimethoxybenzoate, the ACh-induced rise in both pHi and cytosolic Ca2+ concentration was suppressed. ACh and ionomycin caused an increment of amiloride-sensitive acid output into the extracellular fluid, while 20 μmol/l 1-oleoyl-2-acetylglycerol had little effect on it. It was concluded that (a) stimulation with ACh activated the Na+/H+ antiport in the plasma membrane, (b) ACh also stimulated the intracellular acid production but acid extrusion by the Na+/H+ antiport prevented the cell from intracellular acidification, and (c) the major route of signal transduction for the ACh-induced activation of the Na+/H+ antiport was independent of protein kinase C but was dependent on the rise in cytosolic Ca2+ concentration. The implication of the cytosolic acidification and cell volume change in pHi regulation is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00371116
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  • 3
    Electronic Resource
    Electronic Resource
    Cluster cations ejected from liquid metal ion source: alkali metals (Li, Na) and group IV elements (Si, Ge, Sn, Pb) (1989)
    Springer
    The European physical journal 12 (1989), S. 225-227 
    Details
    ISSN: 1434-6079
    Keywords: 36.40 ; 79.70 ; 35.20.X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Cluster abundance of Li n + (n≤19), Na n + (n≤25), Si n z+ (n≤8 forz=1, 3≤n≤7 forz=2), Ge n z+ (n≤11 forz=1, 3≤n≤9 forz=2,n=4 forz=3), Sn n z+ (n≤7 forz=1, 3≤n≤9 forz=2,n=4 forz=3) and Pb n z+ (n≤6 forz=1, 5≤n≤7 forz=2) ejected from a liquid metal ion source has been investigated by mass spectrometry. The abundance spectra of alkali metal clusters showed distinct maxima and steps atn=3, 7, 9, 13 and 19 for Li, and atn=3, 5, 11, 13 and 19 for Na. Mass spectra of Si, Ge and Sn clusters were very similar each other, showing intensity drops aftern=4 and 6 (and alson=10 for Ge) for singly charged clusters. The magic numbers observed are discussed in terms of stability of charged clusters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01426943
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  • 4
    Electronic Resource
    Electronic Resource
    Abundance of Na cluster ions ejected from a liquid metal ion source (1989)
    Springer
    The European physical journal 11 (1989), S. 87-91 
    Details
    ISSN: 1434-6079
    Keywords: 36.40 ; 79.70 ; 35.20.X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Sodium cluster ions Na+ n withn ranging up to 25 have been observed from a liquid sodium ion source by using a magnetic mass analyzer. Ion intensity as a function of cluster size showed distinct steps and local maxima atn=3, 5, 11, 13 and 19 (magic numbers), and a pronounced odd-even alternation. The features in the ion abundance curve are attributed to the relative stability of cluster ions. The observed magic numbers are only partially explained by the electronic shell model, indicating need to include a consideration of atomic structure in a cluster.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01436588
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    Paper (German National Licenses)
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