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Atypical mitotic figures and the mitotic index in cervical intraepithelial neoplasia (1995)

Leeuwen, A. M. ; Burger, P. M. ; Pieters, W. J. L. M. ; [et al.]

Springer

Virchows Archiv 427 (1995), S. 139-144

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ISSN: 1432-2307

Keywords: Mitoses ; Atypical mitotic figures ; Cervical neoplasia

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract We surveyed cervical intraepithelial neoplasia (CIN) to quantify the proliferation rate and the presence of normal and atypical mitotic figures. In the cervical tissue specimens of 127 women with CIN, the area with the highest cell proliferation was identified and, at that site, the proliferation rate was assessed by calculating the mitotic index (MI). Lesions with an MI 〈2 were not considered further. In the area with the highest proliferation rate, 228 mitoses were classified into one of the following groups: normal mitotic figures (NMFs), lag-type mitoses (LTMs) comprising three group metaphases (3GMs), two group metaphases (2GMs) and other lag-type mitoses (OLTMs), multipolar mitoses (MPMs) comprising tripolar mitoses (3PMs) and quadripolar mitoses (4PMs), and other atypical mitotic figures (OAMFs). The median value of the MI increased significantly from 3 in CIN I through 4 in CIN II to 9 in CIN III (P〈0.001). The occurrence of the different LTMs was mutually correlated. The frequency of LTMs increased significantly with increasing CIN grade (P〈0.001), whereas the frequency of NMFs decreased significantly with increasing CIN grade (P〈0.001). The frequency of OAMFs was not related to CIN grade (P=0.94). MPMs were present in low numbers in a minority of the lesions. Spearman's rank correlation coefficient (with 95% confidence limits) between the MI and the number of LTMs, OAMFs and NMFs was 0.66 (0.53; 0.75), −0.14 (−0.32; 0.05) and −0.51 (−0.63; −0.35), respectively. Increasing CIN grade is associated with increasing MI, increasing numbers of LTMs, and decreasing numbers of NMFs. MPMs are very rare events in CIN. The abundant presence of OAMFs seems to be independent of CIN grade and MI.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00196518

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Preparation of biological tissue sections for correlative ion, electron, and light microscopy (1984)

Kupke, K. G. ; Pickett, J. P. ; Ingram, P. ; [et al.]

New York, NY : Wiley-Blackwell

Journal of Electron Microscopy Technique 1 (1984), S. 299-309

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ISSN: 0741-0581

Keywords: Electron microscopy ; Ion microscopy ; Correlative microscopy ; Electron probe microanalysis ; Life and Medical Sciences ; Cell & Developmental Biology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Natural Sciences in General

Notes: In order to correctly interpret the chemical images obtained using ion microscopy (IM), it is useful to correlate them with the information provided by conventional light microscopy (LM), secondary electron imaging (SEI), backscattered electron imaging (BEI), and electron probe microanalysis (EPMA). Accordingly, we have devised a technique of specimen preparation which allows for the application of several different microanalytical techniques to a single histologic section mounted on the same substrate. Sections are cut onto polyester plastic coverslips (devoid of peaks for any element with atomic number 〉 9 using EPMA) and studied by LM. After a light rotary coating with carbon (to prevent charging), the section can then be examined by SEI, BEI, and EPMA. Specific areas can be marked for IM study either with an objective-mounted pin tissue microlocater, or by placing small pieces of metal foil, cut in specific geometric shapes, over features of interest. After sputter-coating the sample with platinum, metal-free shadows are visible using a low-power reflected light microscope available on a typical IM sample chamber as a guide for ion beam placement. The conductive coatings also minimize specimen charging during IM. Post-IM light microscopy, SEI, and BEI are used to confirm the location of specific areas probed in the IM experiments and to provide information on differential ion-sputtering artifacts and tissue contaminants. This new correlative technique should permit better understanding of the images obtained with these diverse instruments.

Additional Material: 7 Ill.