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  • Chemistry  (10)
  • Cytoskeleton  (4)
  • Eye morphogenesis  (2)
  • 1
    Digitale Medien
    Digitale Medien
    Mersalyl, a sulfhydryl reagent, alters the solubility of myosin and cytoskeletal proteins of human platelets
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 880 (1986), S. 197-203 
    Details
    ISSN: 0304-4165
    Schlagwort(e): (Human platelet) ; Cytoskeleton ; Mersalyl ; Myosin ; Sulfhydryl
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin , Physik
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0304-4165(86)90080-2
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  • 2
    Digitale Medien
    Digitale Medien
    Rapid effects ofphorbol ester on platelet shape change, cytoskeleton and calcium transient
    Amsterdam : Elsevier
    FEBS Letters 206 (1986), S. 273-278 
    Details
    ISSN: 0014-5793
    Schlagwort(e): (Blood platelet) ; Ca^2^+ transient ; Cytoskeleton ; Myosin ; Phorbol ester ; Protein kinase C
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Physik
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(86)80995-4
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  • 3
    Digitale Medien
    Digitale Medien
    Presence of calmodulin in human platelet cytoskeletons and its concentration change upon activation of platelets
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 801 (1984), S. 290-297 
    Details
    ISSN: 0304-4165
    Schlagwort(e): (Human) ; Calmodulin ; Cytoskeleton ; Platelet activation
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin , Physik
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0304-4165(84)90079-5
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  • 4
    Digitale Medien
    Digitale Medien
    Possible involvement of membrane fluidity in helicoidal microfibrillar orientation in the coenocytic green alga,Boergesenia forbesii (1994)
    Springer
    Protoplasma 180 (1994), S. 82-91 
    Details
    ISSN: 1615-6102
    Schlagwort(e): Boergesenia forbesii ; Cellulose synthesizing complex ; Cytoskeleton ; Helicoidal wall ; Membrane fluidity ; Microfibril orientation ; Plasma membrane
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Microfibrillar textures and orientation of cellulose microfibrils (MFs) in the coenocytic green alga,Boergesenia forbesii, were investigated by fluorescence and electron microscopy. Newly formed aplanosporic spherical cells inBoergesenia start to form cellulose MFs on their surfaces after 2 h of culture at 25°C. Microfibrillar orientation becomes random, fountain-shaped, and helicoidal after 2, 4, and 5 h, respectively. The fountain orientation of MFs is usually apparent prior to helicoidal MF orientation and thus may be considered to initiate helicoid formation. Microfibrils continue to take on the helicoidal arrangement during the growth ofBoergesenia thallus. The helicoidal orientation of MFs occurs through gradual counterclockwise change in MF deposition by terminal complexes (TCs) viewed from inside the cell. On the dorsal side of curving TC impressions in helicoidal texture formation on a freeze-fractured plasma membrane, the aggregation of intramembranous particles (IMPs) occurs. Membrane flow may thus possibly affect the regulation of helicoidal orientation inBoergesenia. Following treatment with 3 μM amiprophos-methyl (APM) or 1 mM colchicine, cortical microtubules (MTs) completely disappear within 24 h but helicoidal textures formation is not affected. With 15 μM cytochalasin B or 30 μM phalloidin, however, the helicoidal orientation of MFs becomes random. Treatment with CaCl2 (10 mM) causes the helicoidal MF orientation of cells to become random, but co-treatment with N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide (W-7) (100 mM) prevents this effect, though W-7 has no effect on the helicoidal MF formation. It thus follows that MF orientation inBoergesenia possibly involves actin whose action may be regulated by calmodulin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01379226
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  • 5
    Digitale Medien
    Digitale Medien
    An eye imaginal disc-specific transcriptional enhancer in the long terminal repeat of the tom retrotransposon is responsible for eye morphology mutations of Drosophila ananassae (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 161-166 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Key words Drosophila ananassae ; Om mutations ; tom element ; Retrotransposon ; Eye morphogenesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Optic morphology (Om) mutations of Drosophila ananassae are semidominant, neomorphic and nonpleiotropic, map to at least 22 loci scattered throughout the genome, and are associated with the insertion of the tom retrotransposon. Molecular and genetic analyses have revealed that eye morphology defects of Om mutants are caused by the ectopic or excessive expression of Om genes in the eye imaginal discs of third instar larvae. It is therefore assumed that the tom element carries tissue-specific gene regulatory sequences which enhance expression of the Om genes. In the present study, we examined whether or not the long terminal repeats (LTR) of the tom element contain such an eye imaginal disc-specific enhancer, using D. melanogaster transformants containing a lacZ gene ligated to the tom LTR. Analyses of lacZ gene expression in the eye imaginal discs of third instar larvae of 18 independently established transformant lines showed that the tom LTR was capable of enhancing lacZ expression in all the transformant lines, but the degree of enhancement varied between lines. In addition, the effect of the tom LTR lacZ gene evidently changed when the tom LTR construct was relocated to different chromosomal positions. On the basis of these findings, it is hypothesized that ectopic and excessive expression of the Om genes in the eye imaginal discs is induced by an eye imaginal disc-specific enhancer present in the tom LTR, the effect of which may be subject to chromosomal position effects.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02172914
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  • 6
    Digitale Medien
    Digitale Medien
    An eye imaginal disc-specific transcriptional enhancer in the long terminal repeat of thetom retrotransposon is responsible for eye morphology mutations ofDrosophila ananassae (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 161-166 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ananassae ; Om mutations ; tom element ; Retrotransposon ; Eye morphogenesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Optic morphology (Om) mutations ofDrosophila ananassae are semidominant, neomorphic and nonpleiotropic, map to at least 22 loci scattered throughout the genome, and are associated with the insertion of thetom retrotransposon. Molecular and genetic analyses have revealed that eye morphology defects ofOm mutants are caused by the ectopic or excessive expression ofOm genes in the eye imaginal discs of third instar larvae. It is therefore assumed that thetom element carries tissue-specific gene regulatory sequences which enhance expression of theOm genes. In the present study, we examined whether or not the long terminal repeats (LTR) of thetom element contain such an eye imaginal disc-specific enhancer, usingD. melanogaster transformants containing alacZ gene ligated to thetom LTR. Analyses oflacZ gene expression in the eye imaginal discs of third instar larvae of 18 independently established transformant lines showed that thetom LTR was capable of enhancinglacZ expression in all the transformant lines, but the degree of enhancement varied between lines. In addition, the effect of thetom LTRlacZ gene evidently changed when thetom LTR construct was relocated to different chromosomal positions. On the basis of these findings, it is hypothesized that ectopic and excessive expression of theOm genes in the eye imaginal discs is induced by an eye imaginal disc-specific enhancer present in thetom LTR, the effect of which may be subject to chromosomal position effects.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02172914
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  • 7
    Digitale Medien
    Digitale Medien
    Metabolic engineering of cultured tobacco cells (1998)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 58 (1998), S. 329-332 
    Details
    ISSN: 0006-3592
    Schlagwort(e): tobacco cultured cells ; heat-shock promoter of Arabidopsis thaliana ; strong promoter from tobacco cell ; β-glucuronidase ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Construction of a gene expression system in tobacco cultured cells (BY2) was studied. A 925 bp promoter fragment of a heat-shock protein gene (HSP18.2) of Arabidopsis thaliana showed clear heat-shock response of expression of the β-glucuronidase (GUS) reporter gene in BY2 cells. Similar results were observed in a 500 mL flask and 3-L jar fermentor.Isolation of strong promoters in BY2 cells was tried. cDNA clones, in which the mRNA level is high in log-phase cells and the copy number in the genome is low, were isolated. These clones showed high homology with F1-ATPase (mitochondria type), elongation factor 1-α, and a gene with an unknown function of A. thaliana (clone 27), respectively. A 5′-flanking region of clone 27 showed 6.2 times the promoter activity of the CaMV35S promoter in BY2 cells.Three cDNA clones, which are expressed in the stationary growth phase of BY2 cells, were isolated by a differential screening. These clones showed high sequence homologies to alcohol dehydrogenase, pectin esterase, and extensin. Promoters of these genes will be useful in gene expression in high cell-density culture. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:329-332, 1998.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    A study of surface dynamics of polymers. III. Surface dynamic stabilization by plasma polymerization (1988)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 26 (1988), S. 2061-2074 
    Details
    ISSN: 0887-6266
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: As demonstrated in Part II of this series of studies, the hydrophobic character of CF4 plasma-treated Nylon 6 and poly(ethylene terephthalate) (PET) decay with time of water immersion, and the rate of decay can be used as a measure for the surface mobility of (substrate) polymers. The same method of using fluorine-containing moieties introduced by CF4 plasma treatment as surface labeling is applied to investigate the influence of a thin layer of plasma polymer of methane applied onto the surface of those polymers. An ultrathin layer of plasma polymer provides a barrier to the rotational and diffusional migration of the introduced chemical moieties from the surface into the bulk of the film. The influence of operational parameters of plasma polymerization on the surface dynamic stability are examined by measuring the decay rate constants for (subsequently) CF4 plasma-treated samples. The rate constant was found to decrease sharply with increasing value of plasma energy input manifested by J/kg monomer, and no decay was observed as the energy input reached a threshold value (about 6.5 GJ/kg for PET, about 7.0 GJ/kg for Nylon 6), indicating that unperturbable surfaces can be created by means of plasma polymerization.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/polb.1988.090261004
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  • 9
    Digitale Medien
    Digitale Medien
    A study of surface dynamics of polymers. II. Investigation by plasma surface implantation of fluorine-containing moieties (1988)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 26 (1988), S. 1781-1794 
    Details
    ISSN: 0887-6266
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: Macromolecules at the surface of a polymeric solid have considerable mobility, and the specific arrangement of functional groups of macromolecules at the surface is dictated by the environmental conditions in which the surface is placed. Consequently, the change of environmental conditions, such as immersion in water or placement in a biological surrounding, could cause a cosiderable degree of change in the surface characteristics of a polymer from those evaluated in the laboratory against ambient air. The mobile nature of a polymer surface can be investigated by surface-implanting fluorine-containing moieties, mainly - CF3, by the plasma implantation technique and following the disappearance and reappearance of fluorine atoms on the surface. The disappearance rates (based on the immersion time in water at room temperature) of ESCA F1s signals, the decay rates of (advancing) contact angle of water, and the recovery of these values on heat treatment of water-immersed samples were measured as a function of crystallinity of polymer samples (at three levels of crystallinity) for poly(ethylene terephthalate) and nylon 6.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/polb.1988.090260817
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  • 10
    Digitale Medien
    Digitale Medien
    Development of a new advanced process for manufacturing polyacetal resins. Part III. End-capping during polymerization for manufacturing acetal homopolymer and copolymer (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 50 (1993), S. 1317-1329 
    Details
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: Conventionally, acetal homopolymer or copolymer is obtained by the polymerization of formaldehyde or trioxane, following the end-capping using acetic anhydride or unzipping of the unstable polymer end fraction. First, Asahi Chemical developed a new process to obtain an end-capped polymer during polymerization of highly purified formaldehyde using acetic anhydride as the chain-transfer agent. Use of highly purified formaldehyde and endcapping during polymerization using acetic anhydride as a chain-transfer agent or an endcapping agent will provide a simple process for manufacturing acetal homopolymer. The polymerization mechanism was confirmed by infrared spectroscopy analysis and proton NMR analysis of the polymer obtained. Second, for the acetal copolymer, purified trioxane was copolymerized with ethylene oxide in the presence of methylal, which gave an endcapped polymer with high thermal stability. Two new intermediates from the initiation reaction of the copolymerization, 1,3,5,7-tetraoxacyclononane (TOCN) and 1,3,5,7,10-pentaoxacyclododecane (POCD), were isolated and a new initiation mechanism was proposed. © 1993 John Wiley & Sons, Inc.
    Zusätzliches Material: 16 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/app.1993.070500803
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