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  • 1
    Electronic Resource
    Electronic Resource
    Histochemical methods for the further characterisation of the tumourettes of the posterior lobe of the pituitary (1980)
    Springer
    Acta neuropathologica 49 (1980), S. 101-103 
    Details
    ISSN: 1432-0533
    Keywords: Tumourettes of the neurohypophysis ; Granular cell tumours ; Sialomucopolysaccharides ; Neuraminidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The granular cell tumourettes of the posterior lobe of the pituitary possess neuraminic acid containing carbohydrate. After removal of neuraminic acid with neuraminidase and exposure to FITC (Fluorescein isothiocyanate) labelled peanut agglutinin (Arachis hypogaea) (PNA), intracellular receptor structures could be demonstrated. The significance of the findings is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690749
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  • 2
    Electronic Resource
    Electronic Resource
    Histochemical methods for the demonstration of thomsen-friedenreich antigen in cell suspensions and tissue sections (1978)
    Springer
    Journal of molecular medicine 56 (1978), S. 761-765 
    Details
    ISSN: 1432-1440
    Keywords: Thomsen-Friedenreich Antigen ; Erdnußagglutinin ; Neuraminidase ; Autoradiographie ; Fluorescenzmikroskop ie ; Thomsen-Friedenreich antigen ; Peanut agglutinin ; Neuraminidase ; Autoradiography ; Fluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary 1. Three different methods are described for the visualisation of the Thomsen-Friedenreich (TF) antigen on cell suspensions, formalin-fixed and paraffin embedded or frozen tissue sections: a) Rosette formation with chicken and sheep erythrocytes. b) Fluorescence-microscopy with fluorescein labelled peanut agglutinin. c) Autoradiography with3H-labelled peanut agglutinin. 2. The TF antigen was shown, as far as presently investigated, to be exposed on various blood cells, glomerula of the kidney and normal mammary gland after neuraminidase treatment. Mammary gland was also shown to possess TF receptors without prior treatment with neuraminidase. 3. The exposure of this cryptantigen can be brought about by bacterial or viral neuraminidase and is followed by an antigen/antibody reaction, which can lead to possible pathological consequences.
    Notes: Zusammenfassung 1. Drei verschiedene Methoden zur Darstellung von sog. Thomsen-Friedenreich (TF)-Antigenen in Zellsuspensionen, an Formalin-fixiertem und in Paraffin eingebettetem Gewebe sowie in Gefrierschnitten werden beschrieben: a) Rosetten-Bildung mit Hühner- und Schaferythrocyten. b) Fluorescenzmikroskopie mit Fluorescein-markiertem Erdnußagglutinin. c) Autoradiographie mit3H-markiertem Erdnußagglutinin. 2. Nach Neuraminidasebehandlung konnten TF-Antigene — soweit bisher untersucht — auf verschiedenen Blutzellen, in Nierenglomerula und in normalem Brustdrüsengewebe nachgewiesen werden. Ein Teil derartiger Antigene lag im Brustdrüsenparenchym auch bereits als freie Rezeptoren vor, d. h. waren nicht von Neuraminsäure bedeckt. 3. Die Freilegung dieser Kryptantigene kann im Rahmen von Infektionen durch bakterielle und virale Neuraminidase erfolgen. Dabei kommt es zu einer Antigen/Antikörper Reaktion mit unter Umständen klinisch relevanten Folgen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01476765
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  • 3
    Electronic Resource
    Electronic Resource
    The presence and significance of the Thomsen-Friedenreich antigen in mammary gland (1979)
    Springer
    Journal of cancer research and clinical oncology 93 (1979), S. 205-214 
    Details
    ISSN: 1432-1335
    Keywords: Thomsen-Friedenreich antigen ; Breast carcinoma ; Histochemistry ; Lectin ; Neuraminidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Normal tissue as well as various benign and malignant lesions of the breast were histochemically examined for the presence of the Thomsen-Friedenreich (TF)-antigen. Fluorescein- or 3H-labelled peanut agglutinin was used for this purpose, a lectin that is known to have a high affinity for the TF-antigen. The occurrence of this TF-antigen seemed in all cases, even in the carcinoma lobulare in situ that is regarded as being derived from myoepithelial cells by some authors, to be associated with a secretory condition. Its presence (free and neuraminicacid covered) in normal, hyperplastic and malignant breast tissue, however, cannot be considered a specific tumour associated antigen as has been previously assumed. Furthermore the investigations have shown that the intensity of fluorescence for peanut agglutinin (PNA)-receptors was generally stronger in differentiated carcinomas than in undifferentiated carcinomas of the breast. The histochemical findings are discussed with regard to diagnostical and immunotherapeutical aspects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00406579
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  • 4
    Electronic Resource
    Electronic Resource
    Lectin binding sites in the choroid plexus and choroid plexus papillomas (1980)
    Springer
    Neurosurgical review 3 (1980), S. 57-65 
    Details
    ISSN: 1437-2320
    Keywords: Choroid plexus ; Choroid plexus papilloma ; Papillary ependymoma ; Brain metastasis ; Pneumococcal meningitis ; Colloidal iron reaction ; Lectin receptors ; Neuraminic acid mucopolysaccharides ; Neuraminidase ; Plexus chorioideus ; Papillom des Plexus chorioideus ; papilläres Ependymom ; Ca-Metastase ; Pneumokokken-Meningitis ; kolloidale Eisenreaktion ; Lektinrezeptoren ; Sialo-Mucopolysaccharide ; Neuraminidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die luminale Oberfläche der Zellen des Plexus chorioideus und der Plexuspapillome ist mit einem Überzug aus Sialo-Mucopolysacchariden bedeckt, der mit der kolloidalen Eisenreaktion nach Hale darstellbar ist und mit Neuraminidase entfernt werden kann. Nach Neuraminidase-Vorbehandlung lassen sich an den Zelloberflächen mit Fluorochrommarkierten Lektinen der Erdnuß (PNA) und von Rhicinus communis (RCA) spezifische, bestimmten Kohlehydraten entsprechende Rezeptoren nachweisen. Die Identität der PNA-Rezeptoren mit dem Thomsen-Friedenreich Antigen legt deren immunologische Bedeutung bei bakteriellen oder viralen Infektionen nahe. Die Anwendung dieser histochemischen Methoden scheint darüber hinaus eine differentialdiagnostische Klärung zwischen Plexuspapillomen und anderen papillären Tumoren (Ca-Metastasen, papilläre Ependymome) zu ermöglichen.
    Notes: Summary The surface of the cells of the normal choroid plexus and of the plexus papillomas is coated by sialomucopolysaccharides, containing substances which are positively stained with the colloidal iron (Hale-) reaction. After pretreatment with neuraminidase sialic acid is removed rendering the membrane negative to the Hale reaction. Using FITC- or rhodamine-labelled PNA (Arachis hypogaea) and RCA (Rhicinus communis) lectins specific receptors are demonstrable. The identity of these distinct oligosaccharides containing receptors with the Thomsen-Friedenreich antigens suggests the possibility of an immunologic significance, e. g. in bacterial or viral infections of the brain. The application of histochemical techniques seem suitable to clarify the differential-diagnosis between choroid plexus papillomas on the one hand and metastasis of carcinoma and papillary ependymomas on the other hand.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01644420
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