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  • Organelle movement  (3)
  • Key words Pollen tube  (2)
  • Key words Nicotiana tabacum  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Dynein heavy chain-related polypeptides are associated with organelles in pollen tubes of Nicotiana tabacum (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 31-40 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Key words Pollen tube ; Nicotiana tabacum ; Dynein heavy chain related polypeptides ; Immunolocalization
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  It is known that pollen tubes contain two high molecular weight polypeptides which share some biochemical and immunological properties with dynein heavy chains. This paper reports data on the subcellular localization of the two dynein heavy chain-related polypeptides during pollen tube growth. Immunofluoresence studies using a purified antibody (Dy-1) raised against a synthetic peptide reproducting the P-loop conserved sequence of dynein heavy chains showed spot-like structures, with a characteristic distribution pattern that depended on the tube length. Biochemical evidence confirmed the presence of dynein heavy chain-related bands in the pollen tube membrane fraction. The association of proteins carrying dynein heavy chain-related polypeptides to cell membranes was affected by detergent (Triton×100), whereas other stripping agents, like NaCl and Na2CO3, did not significantly influence the interaction of dynein heavy chain-related doublet with their cytoplasmic targets. These data suggest that dynein heavy chain-related polypeptides associate with membranous organelles within the vegetative cell of Nicotiana tabacum pollen tubes, implying their involvement in the cytoplasmic distribution of these organelles.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004970050118
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  • 2
    Digitale Medien
    Digitale Medien
    In vitro double fertilization in Nicotiana tabacum (L.): fusion behavior and gamete interaction traced by video-enhanced microscopy (2000)
    Springer
    Sexual plant reproduction 12 (2000), S. 267-275 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Key words Nicotiana tabacum ; In vitro fertilization ; Gamete interaction ; Cytology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  In vitro double fertilization in tobacco was carried out with attention to fusion behavior and gamete interaction. Structural and cytological events indicating possible reaction to the fusion of sperm-egg and especially sperm-central cell were recorded by video-enhanced microscopy. Generative cells were fused with the egg cell or central cell as a control system to better understand gamete interaction. As early as adherence of the male cell, the female cell showed response by means of cytoplasm strand formation. After gamete fusion, cytoplasm activation in the egg cell was observed as long distance movement of organelles. In fertilized central cells, however, fusion did not result in notable cytological change within 30 min. Male nuclear movement recorded in the female cell illustrated two different patterns of movement which showed similarity to organelle movement. The dynamics of male and female nuclear fusion after in vitro fertilization was also recorded in the central cell. It revealed that the fusion process requires only a few seconds and is similar to that of gamete fusion in vitro. This may offer a new clue for understanding how female and male nuclei attract, adhere and finally fuse each other.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004970050194
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  • 3
    Digitale Medien
    Digitale Medien
    Cytoplasmic motors and pollen tube growth (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 59-64 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Motor proteins ; Pollen tube ; Tip growth ; Organelle movement
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The growth of pollen tubes is characterized by an intense cytoplasmic streaming, during which the movements of smaller organelles (like secretory vesicles) and larger ones (including the generative cell and vegetative nucleus) are precisely coordinated. A well-characterized cytoskeletal apparatus is likely responsible for these intracellular movements. In recent years both microfilament and microtubule-based motor proteins have been identified and assumed to be the translocators of the several organelle categories. Their precise function during pollen tube growth is not yet clear, but apparently an actomyosin-based system is mainly responsible for pollen tube elongation. On the other hand, microtubules and microtubule-based motors have been thought to play a role in the maintenance of cell polarity. Both cytoskeletal systems (and their respective motor activities) could cooperate to ensure a precise regulation of pollen tube growth.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02153052
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  • 4
    Digitale Medien
    Digitale Medien
    The kinesin-immunoreactive homologue from Nicotiana tabacum pollen tubes: Biochemical properties and subcellular localization (1993)
    Springer
    Planta 191 (1993), S. 496-506 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Kinesin ; Nicotiana ; Organelle movement ; Pollen tube
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract In plant cells, microtubule-based motor proteins have not been characterized to the same degree as in animal cells; therefore, it is not yet clear whether the movement of organelles and vesicles is also dependent on the microtubular cytoskeleton. In this work the kinesinimmunoreactive homologue from pollen tubes of Nicotiana tabacum L. has been purified and biochemically characterized. The protein preparation mainly contained a polypeptide with a relative molecular weight of approx. 100 kDa. This polypeptide bound to animal microtubules in an ATP-dependent manner and it further copurified with an ATPase activity fourfold-stimulated by the presence of microtubules. In addition, the sedimentation coefficient (approx. 9S) was similar to those previously shown for other kinesins. Immunofluorescence analyses revealed a partial co-distribution of the protein with microtubules in the pollen tube. These data clearly indicate that several properties of the kinesin-immunoreactive homologue are similar to those of kinesin proteins, and suggest that molecular mechanisms analogous to those of animal cells may drive the microtubule-based motility of organelles and vesicles in plants.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00195751
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  • 5
    Digitale Medien
    Digitale Medien
    Cytoplasmic motors and pollen tube growth (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 59-64 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Key words Motor proteins ; Pollen tube ; Tip growth ; Organelle movement
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The growth of pollen tubes is characterized by an intense cytoplasmic streaming, during which the movements of smaller organelles (like secretory vesicles) and larger ones (including the generative cell and vegetative nucleus) are precisely coordinated. A well-characterized cytoskeletal apparatus is likely responsible for these intracellular movements. In recent years both microfilament- and microtubule-based motor proteins have been identified and assumed to be the translocators of the several organelle categories. Their precise function during pollen tube growth is not yet clear, but apparently an actomyosin-based system is mainly responsible for pollen tube elongation. On the other hand, microtubules and microtubule-based motors have been thought to play a role in the maintenance of cell polarity. Both cytoskeletal systems (and their respective motor activities) could cooperate to ensure a precise regulation of pollen tube growth.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004970050011
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  • 6
    Digitale Medien
    Digitale Medien
    Dynein-related polypeptides in pollen and pollen tubes (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 312-317 
    Details
    ISSN: 1432-2145
    Schlagwort(e): Key words Pollen tube ; Nicotiana tabacum ; Ginkgo biloba ; Dynein
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Microtubules in pollen tubes are evident within the vegetative and generative cell cytoplasm. This observation led to the formulation of several hypotheses regarding the role of microtubules in cytoplasmic movement and the migration of the vegetative nucleus/generative cell along the pollen tube. The study of microtubular motor proteins in pollen tubes followed the discovery and characterization of an immunoreactive homolog of mammalian kinesin in tobacco pollen tubes. Recent identification of dynein-related polypeptides in pollen tubes of Nicotiana tabacum and pollen of Ginkgo biloba is a significant step in the definition of the role of microtubule function within pollen and pollen tubes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004970050048
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