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  • 1
    Digitale Medien
    Digitale Medien
    Isozyme variation and RFLPs at the β-amylase loci in wheat (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 691-699 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Hexaploid wheat ; β-Amylase ; Isozymes ; RFLPs ; IEF
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Forty-one hexaploid wheat genotypes have been examined for RFLPs detected by a β-amylase probe using three restriction enzymes, and for mature grain β-amylase isozyme polymorphism following IEF. The two homoeoallelic series assayed for RFLPs differed: little variation was found at group 2 chromosome homoeoloci, while the group 4/5 chromosome homoeoloci displayed considerable variation. Varieties that displayed a RFLP with one RE almost always did likewise with the other two REs, suggesting that most of the polymorphisms observed were due to large DNA rearrangements. Comparison of the variation in grain β-amylase isozymes with the RFLP results indicated strong associations between particular RFLP and isozyme alleles.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00303514
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  • 2
    Digitale Medien
    Digitale Medien
    The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 342-348 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Hexaploid wheat ; RFLPs ; Homoeology ; Chromosome arm ; Alien additions
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary To investigate the use of RFLP analysis in the Triticeae, a set of low copy number probes has been isolated from a wheat cDNA library. The probes identify each of the 14 homoeologous chromosome arms of wheat as determined by analysis of DNA fragments hybridizing to the probes in aneuploid lines of Chinese Spring. These probes can be used in RFLP analyses both for the assignment of homoeology of alien chromosomes or arms added to wheat, and for the determination of chromosome dosage in wheat aneuploids. Different chromosomes from various Triticeae species can therefore be followed in a wheat genetic background using a single technique. The potential uses of the set in facilitating the transfer of alien segments into wheat are outlined.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00265294
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  • 3
    Digitale Medien
    Digitale Medien
    Molecular cloning and chromosomal location of genes encoding the “Early-methionine-labelled” (Em) polypeptide of Triticum aestivum L. var. Chinese Spring (1990)
    Springer
    Theoretical and applied genetics 80 (1990), S. 43-48 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Wheat ; Em genes ; Group 1 chromosomes ; RFLPs
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The “Early-methionine-labelled” (Em) polypeptide is the most abundant cytosolic polypeptide found in mature wheat embryos. Using a near full-length cDNA clone as a hybridisation probe to detect genomic sequences by Southern blotting of electrophoretic separations of genomic DNA derived from Triticum aestivum L. var. Chinese Spring and a series of its aneuploid derivatives, we demonstrate that the Em polypeptide is the product of a small multigene family in which the copies are located on each of the long arms of the homoeologous group 1 chromosomes. Screening of a variety of genotypes additionally reveals a number of restriction fragment length polymorphisms associated with these loci. Screening of a library of genomic DNA cloned in the vector λEMBL 4 has resulted in the isolation of a genomic fragment containing two closely linked Em genes. These are separated by ca. 2.5 kb. Analysis of restriction enzyme digests of this clones fragment has identified it as originating from chromosome 1A.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224014
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  • 4
    Digitale Medien
    Digitale Medien
    Treatment of infantile phytanic acid storage disease: clinical, biochemical and ultrastructural findings in two children treated for 2 years (1988)
    Springer
    European journal of pediatrics 147 (1988), S. 133-142 
    Details
    ISSN: 1432-1076
    Schlagwort(e): Infantile Refsum disease ; Phytanic acid ; Dietary treatment ; Peroxisomes ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Two patients with infantile phytanic acid storage disease (infantile Refsum disease), one of whom showed the presence of morphologically normal peroxisomes in a liver biopsy, were treated with a low phytanic acid diet for more than 2 years and the effects of treatment on certain clinical, biochemical and ultrastructural parameters were examined. Both patients showed evidence of either an improvement or stabilisation in their clinical condition. Plasma phytanic acid levels decreased to near normal values in approximately 6 weeks after the introduction of the diet; plasma pipecolic acid also declined markedly but the decrease was not so rapid and its level remained abnormal. C26∶C22 fatty acid ratios decreased very slowly and even after 2 years the values remained grossly abnormal. Despite the marked reduction of phytanic acid in the liver, there was an increase in the C26∶C22 fatty acid ratios and this appeared to be paralleled by an increase in inclusion bodies. Our data suggest that some patients with the infantile form of Refsum disease may show some clinical benefit from dietary management and this is reflected biochemically by decreases in the plasma levels of phytanic acid and pipecolic acid.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00442210
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  • 5
    Digitale Medien
    Digitale Medien
    RFLP-based genetic maps of wheat homoeologous group 7 chromosomes (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 495-504 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Wheat ; Genetic mapping ; RFLPs ; Isozymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Restriction fragment length polymorphism (RFLP) mapping was attempted using 18 cDNA clones, 14 anonymous and 4 of known function, which had been shown to have homologous DNA sequences on the group 7 chromosomes of wheat. The loci identified by these probes have been mapped on one or more chromosomes in this homoeologous group using linkage data derived from various F2, random inbred, doubled haploid and single chromosome recombinant populations. The maps also include three isozyme loci, five disease resistance loci, two anthocyanin pigment loci and a vernalisation response locus. The mapping data have been used to determine the extent of map co-linearity over the A, B and D genomes, the degree of RFLP variability in the three genomes and the relative efficiency of various restriction enzymes in detecting RFLPs in wheat. The strategy for future mapping in wheat, particularly the use of “alien” genomes or segments, such as that from Aegilops ventricosa used here, is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00290833
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  • 6
    Digitale Medien
    Digitale Medien
    Location of β-amylase sequences in wheat and its relatives (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 286-290 
    Details
    ISSN: 1432-2242
    Schlagwort(e): RFLP ; Wheat ; Aegilops ; Rye ; β-Amylase
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A β-amylase cDNA clone isolated from barley has been used to locate β-amylase encoding sequences on wheat, rye, and Aegilops umbellulata chromosomes by hybridisation to restriction endonuclease digested DNA obtained from wheat aneuploid and wheat-alien addition lines. Structural genes were identified on homoeologous group 4 and 5 chromosomes, confirming the results of isozyme studies. In addition, a further set of structural genes was found on homoeologous group 2 chromosomes. It is proposed that there are two homoeoallelic series, β-Amy-1 on group 4 or 5 chromosomes, and β-Amy-2 on group 2 chromosomes. Evidence is presented that each locus contains one or two β-amylase structural genes, and it is suggested that the large number of isozymes seen upon IEF are due to post-translational modifications.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00303966
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  • 7
    Digitale Medien
    Digitale Medien
    Immunofluorescent localization and ultrastructural characterization of gonadotrophe cells in the adenohypophysis of the barbary drake (Cairina moschata L.) using anti-chicken LH serum (1977)
    Springer
    Cell & tissue research 181 (1977), S. 531-544 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Adenohypophysis, duck ; Gonadotrophes ; Immunofluorescence ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary An indirect immunofluorescence technique and an anti-chicken LH serum were used to localize cells in the adenohypophyses of drakes at different stages of their breeding cycle, after castration, and after castration combined with thyroxine treatment. Immunofluorescent cells were distributed throughout both lobes of the adenohypophyses from control and experimental birds and were shown to be alcian blue positive, PAS negative, basophiles. Immunofluorescent cells were as numerous in castrated birds as in castrated birds treated with thyroxine. Adjacent thin and semi-thin sections were used to study the cells binding anti-LH serum at light microscope and ultrastructural levels. The cells contained spherical granules with variable densities and diameters ranging between 40 and 280 nm in the rostral (= cephalic) lobe, and between 60 and 260 nm in the caudal lobe. The light microscope and ultrastructural observations showed that the anti-LH serum binds to cells which have been classified by other authors in the Pekin duck, quail and pigeon as TSH producing delta cells. The experimental technique used did not permit a distinction to be made between cells producing FSH and LH.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221774
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