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  • 1975-1979  (6)
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Materialart
Erscheinungszeitraum
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  • 1
    Digitale Medien
    Digitale Medien
    Anomeric preferences ofd-glucose uptake and utilization by cerebral cortex slices of rats (1979)
    Springer
    Neurochemical research 4 (1979), S. 505-516 
    Details
    ISSN: 1573-6903
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract On aerobic incubation of rat cerebral cortex slices with anomers ofd-glucose and with 2-deoxy-d-glucose (2DG) for 5 min, the disappearance of β-d-glucose from the incubation mixture was greater than that of α-d-glucose and both anomers had a greater rate of disappearance than that of 2DG. In addition, there were significantly greater consumption of oxygen and production of lactate with the β-anomer than with the α-anomer. In similar experiments with3H-labeledd-glucose anomers and [1-3H]-3-O-methyl-d-glucose (3MG), the accumulation of [1-3H]-β-d-glucose (up to 5 min) by rat cerebral cortex slices was greater than that of [1-3H]-α-d-glucose. Although initially lower than that of the anomers, the accumulation of [1-3H]-3MG increased at a greater rate and, by 5 min of incubation, was greater than that of both glucose anomers. This preferential accumulation was seen to disappear when the slices were preincubated with 2DG (hexokinase inhibitor) or when the temperature of incubation was reduced to 20°C. Under those conditions the data with the glucose anomers were similar to those obtained with 3MG. Our data then suggested that the greater accumulation of β-d-glucose than of α-d-glucose by the slices was probably not due to differences in transport through brain cell membranes but rather to the preferential metabolism of the β-d-glucose.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00964644
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  • 2
    Digitale Medien
    Digitale Medien
    The localization of mutarotase in rat kidney (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 1094-1097 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The localization of mutarotase in rat kidney was investigated by fluorescein-labelled and peroxidase-labelled antibody techniques, and by method of isolation of the nuclei and cytoplasm in non-aqueous solvents. In these immunohistochemical studies, mutarotase was almost exclusively recognized in the nuclei of epithelial cells of renal tubules and glomeruli in rat. The specific activity of mutarotase was found to be 1.5 times higher in the nuclei (122 units/g dry wt) than that in the cytoplasm (80 units/g dry wt) isolated with non-aqueous solvents. These results suggest that mutarotase may be involved in the metabolism of D-glucose in nuclei.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01949964
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  • 3
    Digitale Medien
    Digitale Medien
    Different effects of D-glucose anomers for respiration of bacterial germinated spores (1979)
    Springer
    Cellular and molecular life sciences 35 (1979), S. 470-472 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Effects of α- or β-D-glucose on the respiration of germinated spores (only germinated spores not including swollen spores and elongated spores) ofBacillus subtilis andB. megaterium were studied. In our conditions, net amount of oxygen consumed by 1010 germinated spores ofB. subtilis per min after addition of α- or β-D-glucose was 1.6 μg or 6.6 μg (β/α=4.13), while that byB. megaterium was 4.5 μg or 6.8 μg (β/α=1.51), respectively. However, the net amounts of oxygen consumed by 1010 vegetative cells per min after addition of α- or β-D-glucose were identical, forB. subtilis in both cases 443.0 μg and forB. megaterium in both cases 604.4 μg.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01922712
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  • 4
    Digitale Medien
    Digitale Medien
    Uptake of D-glucose anomers by rat retina (1977)
    Springer
    Cellular and molecular life sciences 33 (1977), S. 19-20 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Uptake ofd-glucose anomers by isolated rat retina was studied. After 3 min incubation at 37°C in the presence of α or β anomer (750 μg/ml), a significantly greater uptake (1.32 mg/g wet tissue) of β-anomer was observed compared with that of α-d-glucose (1.11 mg/g wet tissue). This result and other data suggest that the carrier ford-glucose transport in the retina prefers the β-anomer stereospecifically.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01936727
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  • 5
    Digitale Medien
    Digitale Medien
    Anomeric compositions of D-glucose in tissues and blood of rat (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 167-169 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The anomeric compositions of D-glucose in the liver, kidney, heart, blood and plasma of rat were determined by our method for the assay of D-glucose anomers and the percentages of the β-anomer were found to be 61.8, 61.0, 62.4, 62.7 and 62.9, respectively.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01944655
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  • 6
    Digitale Medien
    Digitale Medien
    Multiple forms of human kidney mutarotase (1978)
    Springer
    Cellular and molecular life sciences 34 (1978), S. 563-564 
    Details
    ISSN: 1420-9071
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary 4 forms of mutarotase from human kidney were demonstrated by DEAE-cellulose column chromatography. A major form of them was purified to homogeneity.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01936959
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