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Digitale Medien

Design and application of a post-column extraction system compatible with miniaturized liquid chromatography (1984)

Apffel, J. A. ; Brinkman, U. A. Th. ; Frei, R. W.

Springer

Chromatographia 18 (1984), S. 5-10

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Details

ISSN: 1612-1112

Schlagwort(e): Miniaturized HPLC ; Post-Column Extraction Detection ; Segmented-Flow Open-Tubular Reactors ; Membrane Phase Separator ; Anti-Cancer Drugs

Quelle: Springer Online Journal Archives 1860-2000

Thema: Chemie und Pharmazie

Notizen: Abstract An on-line, post-column, extraction system suitable for use with miniaturized HPLC (0.7–1 mm i.d. columns) is described. The system consists of a 30° angle segmenting tee, a PTFE extraction coil of variable length and a membrane phase separator. The internal volume of the phase separator is less than 1 μl. The total variance contribution of the system is between 1 and 6 sec2. The determination of the anti-cancer drugs VP 16 and VM 26 in urine by means of reversed-phase HPLC and fluorescence detection, using both on-column preconcentration and the present miniaturized post-column extraction system is presented as an application.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02279454

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