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  • 11
    ISSN: 1438-2199
    Keywords: Amino acids ; Hydroxyproline ; Dabsyl-chloride ; Urine ; High-performance liquid chromatography ; o-Phthalaldehyde
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In order to analyse hydroxyproline (HYP) in urine, a high-performance liquid chromatographic method was modified. The primary amino groups were blocked with o-phthalaldehyde, and then the secondary amino groups were derivatized with 4-dimethylaminoazobenzene-4′-sulphonyl chloride. In addition, the dabsylated samples were treated with ethyl acetate to obtain a simple elution profile in high-performance liquid chromatography. The dabsyl-HYP and -proline were eluted at 4.7 min and 8.0 min, respectively. The chromatographic analysis was completed within 10 min, including the time needed for reequilibration of the column. Using the present method, the concentration of HYP in urine was determined to 260 ± 6µmol/l.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Journal of neural transmission 100 (1995), S. 263-267 
    ISSN: 1435-1463
    Keywords: Amyotrophic lateral sclerosis ; cerebrospinal fluid ; cyclic GMP ; nitric oxide ; Parkinson's disease ; spinocerebellar ataxia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To investigate whether nitric oxide (NO) plays a role in degenerative neurologic disease (DND), we measured nitrite, nitrate and cyclic GMP in cerebrospinal fluid (CSF) samples from patients with Parkinson's disease (PD), spinocerebellar ataxia (SCA) and amyotrophic lateral sclerosis (ALS). We found no significant change in CSF nitrite, nitrate or cyclic GMP in patients with any DND compared with control values. These results suggest that NO production is preserved in PD, SCA and ALS.
    Type of Medium: Electronic Resource
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    International archives of occupational and environmental health 68 (1996), S. 305-314 
    ISSN: 1432-1246
    Keywords: Key words Blood level ; Cadmium ; Dietary intake Japan ; Lead
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Objective To investigate the current levels of exposure of the Japanese population to cadmium and lead, in comparison with the levels in 1980s. ⋅ Design A nation wide survey was conducted in 1991–1994 (the 1990 study) in 19 study sites in Japan as a follow-up to a study conducted in 1979–1983 (the 1980 study). Blood samples and 24-h total food duplicates were collected from women who did not smoke or drink habitually. Methods Blood and food duplicates (after homogenization) were analysed for cadmium (Cd-B and Cd-F, respectively) and lead (Pb-B and Pb-F) by graphite furnace atomic absorption after wet-ashing. ⋅ Results Altogether, 467 women volunteered for blood sampling. Of these women, 375 also gave food duplicates. Geometric mean (GM) Cd-B levels in the 1990 study were lower than the corresponding 1980 values in most study sites so that the 1990 grand GM (1.98 ng/ml) for Cd-B was significantly lower than the 1980 GM (3.58 ng/ml). This reduction in Cd-B was related to the reduction in Cd-F (GM for Cd-F was 38.0 μg/day in the 1980 study and 30.0 μg/day in the 1990 study). Dietary intake was almost exclusively the route of Cd burden among the populations studied. Both Pb-B and Pb-F also showed a remarkable reduction, i.e. from 33.9 ng/ml (1980 GM) to 23.2 ng/ml (1990 GM) in the case of Pb-B, and from 32.2 μg/day (1980 GM) to 7.1 μg/day (1990 GM) in the case of Pb-F. Pb-B, however, did not correlate with Pb-F either in the 1980 or the 1990 study, because Pb intake via inhalation of air remained significant when compared with dietary intake. ⋅ Conclusion The Cd burden in Japan has decreased markedly in the past 10 years, although it is still higher than in other countries. The Pb burden has been quite low.
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  • 14
    ISSN: 1432-1246
    Keywords: Key words Lead ; Cadmium ; Food ; Blood ; Chinese women ; Japanese women
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objectives: To assess and compare the background exposure of the general population to lead (Pb) and cadmium (Cd) in China and in Japan. Methods: Food duplicates and peripheral blood samples were collected from nonoccupationally exposed subjects, viz 202 Chinese women in four Chinese cities (Beijing, Shanghai, Nanning, and Tainan) and 72 Japanese women in three Japanese cities (Tokyo, Kyoto, and Sendai) in the years 1993–1995. Wet-ashing and graphite furnace atomic absorption spectrometric methods were used for the determination of Pb and Cd levels in food and blood samples. Results: Geometric mean (GM) dietary Pb intake (25.8 μg/day) and the GM Pb concentration in blood (56.7 μg/l) in Chinese were significantly higher than in Japanese women (11.6 μg/day in food and 32.1 μg/l in blood), whereas Cd in food (32.1 μg/day) and Cd in blood (1.92 μg/l) in Japanese were significantly higher than in Chinese women (9.9 μg/day in food and 1.07 μg/l in blood). The intake of Pb and Cd via boiled rice accounted for 3.6% and 31.1% of the total dietary burden in Chinese, and 12.1% and 32.7% in Japanese, respectively. The Cd burden was acquired almost exclusively through the dietary route, whereas the Pb burden came from both air and food, especially in the case of the Chinese population. Conclusions:  The background Pb exposure in the Chinese population was higher than that in the Japanese population, whereas Cd exposure was lower in Chinese women than in their Japanese counterparts.
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  • 15
    ISSN: 1432-1246
    Keywords: Key words Biological monitoring ; 1-Butanol ; Carbon cloth ; Diffusive sampling ; Hydrolysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Objectives: To investigate the possibility of applying diffusive air sampling and urinalysis (for mother compound and metabolites) to the monitoring of exposure of factory workers to 1-butanol. Methods: The performance of carbon cloth in adsorbing 1-butanol vapor in air was studied by experimental exposure of the cloth to 1-butanol at 50, 100, 200 or 400 ppm for up to 10 h. 1-Butanol in the exposed cloth was extracted with carbon disulfide and this was followed by gas-chromatographic (GC) analysis. Urine samples were collected from factory workers occupationally exposed to 1-butanol and from rats exposed experimentally to 1-butanol vapour (up to 200 ppm). The urine samples were analyzed by GC without any pretreatment, or after treatment with hydrochloric acid or hydrolase preparation. Results: The performance of the carbon cloth was such that it adsorbed 1-butanol in proportion to the concentration (up to 400 ppm) and the duration (up to 10 h) of exposure, and responded quantitatively to a 15-min exposure up to 400 ppm. The amount of 1-butanol (after enzymic or acid hydrolysis) in post-exposure urine samples from rats was proportional to the exposure intensity. The proportion of free 1-butanol in total 1-butanol (i.e., free+conjugated) in urine was higher after 100 or 200 ppm exposure (35–40%) than after 50 ppm exposure (about 8%). There was a significant increase in total 1-butanol concentration (but not in free 1-butanol) in shift-end urine samples of workers exposed to 1-butanol at concentrations up to 3 ppm. Conclusions: Diffusive sampling with carbon cloth as an adsorbent can be applied to ambient air monitoring of exposure to 1-butanol. Urinalysis for 1-butanol after hydrolysis is sensitive enough to detect occupational 1-butanol vapour exposure at 3 ppm.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    International archives of occupational and environmental health 68 (1996), S. 289-297 
    ISSN: 1432-1246
    Keywords: Key words o-Cresol ; Exposure marker ; Hippuric acid ; Toluene ; Urinalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Head-space gas chromatography (GC) and high-performance liquid chromatography (HPLC) (with fluorescence detectors) methods were developed for toluene (TOL-U) and o-cresol (CR-U) in urine, respectively. In order to identify the most sensitive urinary indicator of occupational exposure to toluene vapor (TOL-A) among TOL-U, CR-U, and hippuric acid in urine (HA-U), the two methods together with an HPLC (with untraviolet detectors) method for determination of HA-U were applied in the analysis of end-of-shift urine samples from 115 solvent-exposed workers (exposed to toluene at 4 ppm as geometric mean). Regression analysis showed that TOL-U correlated with TOL-A with a significantly higher correlation coefficient than did HA-U or CR-U. With regard to the TOL-A concentrations at which the exposed subjects could be separated from the nonexposed by the analyte, TOL-U achieved separation at 〈10 ppm TOL-A, whereas both HA-U and CR-U did so only when TOL-A was 30 ppm or even higher. The ratio of the analyte concentrations at 50 ppm TOL-A to those at 0 ppm TOL-A was also highest for TOL-U. Overall, the results suggest that TOL-U is a better marker of exposure to toluene vapor than HA-U or CR-U.
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    International archives of occupational and environmental health 72 (1999), S. 516-520 
    ISSN: 1432-1246
    Keywords: Key words Biological monitoring ; Blood lead ; Urinary lead
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Objectives: The aim of the present study is to investigate whether lead (Pb) in urine (Pb-U) can be a valid surrogate of lead in blood (Pb-B), the traditional biomarker of exposure to lead in occupational health. Methods: Blood and spot urine samples were collected from 258 workers of both sexes occupationally exposed to lead. The samples were analyzed for lead by graphite furnace atomic absorption spectrometry, and the correlation between Pb-B and Pb-U was examined by linear regression analysis before and after logarithmic conversion. Results: The correlation coefficient (0.824; P 〈 0.01) was largest when the relationship between Pb-B and Pb-U was examined with 214 cases of one sex (i.e., men) after Pb-U was corrected for a specific gravity (1.016) of urine (Pb-Usg) and both Pb-B and Pb-Usg were converted to logarithms. The geometric means (GMs) of Pb-B and Pb-Usg for the 214 men were 489 μg/l and 81 μg/l, respectively. When Pb-Usg was assumed to be 100 μg/l in this set of correlations, the 95% confidence range of Pb-B for the group mean was narrow, i.e., 543–575 μg/l (with GM of 559 μg/l), whereas that for individual Pb-B values was as wide as 355–881 μg/l. Conclusions: The correlation of Pb-U with Pb-B among workers occupationally exposed to Pb was close enough to suggest that Pb-U may be a good alternative to Pb-B on a group basis, but not close enough to allow Pb-U to predict Pb-B on an individual basis.
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  • 18
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Initial studies of the SI82 gene suggested the existence of other genes with similar sequences1. Searches of nucleotide sequence database by using the BLASTX alignment program uncovered three expressed sequence-tagged sequences (ESTs) (T03796, and subsequently R14600 and ...
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  • 19
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Some cases of Alzheimer's disease are inherited as an autosomal dominant trait. Genetic linkage studies have mapped a locus (AD3) associated with susceptibility to a very aggressive form of Alzheimer's disease to chromosome 14q24.3. We have defined a minimal cosegregating ...
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Surgical endoscopy and other interventional techniques 10 (1996), S. 164-165 
    ISSN: 1432-2218
    Keywords: Foreign body ; Radiolucent ; CT scan ; Flexible fiberscope
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A 25-year-old male patient with a sharp, large, and radiolucent tracheobronchial foreign body which was inhaled at the time of a traffic accident is reported on. CT scan was quite useful in finding this radiolucent foreign body. The patient had no respiratory disturbance because the foreign body was located in the level between bifurcation and left main bronchus; however, a flexible fiberscopic procedure performed to remove the body caused an airway obstruction and a dyspnea because the foreign body lodged in the subglottis. Remarkable progress has been made in the development of the flexible fiberscope system. Almost all medical facilities in Japan have flexible systems. However, the opportunities for young physicians to learn about rigid systems may now become limited. This case may warn us not to have too much confidence in the ability of flexible fiberscope system to remove this kind of large foreign body and remind us of the need to continue adequate training in the rigid systems.
    Type of Medium: Electronic Resource
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