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  • 11
    Digitale Medien
    Digitale Medien
    Screening and selection of media components for lactic acid production using Plackett–Burman design (1998)
    Springer
    Bioprocess engineering 19 (1998), S. 61-65 
    Details
    ISSN: 0178-515X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Plackett–Burman design was used to efficiently select important media components influencing lactic acid production in a two step screening procedure. A total of 36 screening experiments were conducted for studying the effect of various media components such as carbon and nitrogen (simple and complex) sources, minerals/buffering agents and a specific inducer for the production of lactic acid by Lactobacillus plantarum NCIM 2084. The eleven ingredients chosen after the first screening experiments were further screened by a Plackett-Burman design consisting of 12 experiments. Liquefied starch, wheat bran extract, ammonium nitrate, manganese sulphate and sodium acetate were chosen as promising ingredients for further optimisation studies. The highest yield of 41.9 g/l of lactic acid was obtained at the end of 24 hours of fermentation which corresponded to 90% conversion, on the basis of sugar supplied.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/PL00009003
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  • 12
    Digitale Medien
    Digitale Medien
    Scale up studies for the production of biosurfactant in packed column bioreactor (2000)
    Springer
    Bioprocess engineering 22 (2000), S. 95-99 
    Details
    ISSN: 0178-515X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract Biosurfactants capable of emulsifying pesticides have great potential to assist in microbial degradation of the pesticides. Solid State Fermentation (SSF) due to several advantages, is one of the efficient ways of producing these surfactants and seldom receives attention for commercial exploitation. In this study, a packed column bioreactor with wheat bran as the raw material and Bacillus subtilis has been used to produce a biosurfactant specific to disperse Fenthion, an organophosphrous pesticide. The emulsifier activity (EA) and surface tension from the packed column bioreactor were compared with flask fermentation experiments, which served as control. Airflow rate in the packed column bioreactor was varied from 10–20 l/min. Maximum EA and minimum surface tension occurred at airflow rate of 20 l/min. Peak EA in the control was 1.2 at 29 h while it was 1.9 in the bioreactor. The least surface tension of 24 dynes/cm was noticed at 54 h in the bioreactor, which was 33% better than the control at the same time period. The results indicate that the packed column bioreactor can become a more acceptable solid state fermentation system for commercial exploitation of Fenthion specific biosurfactant production.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004490050017
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  • 13
    Digitale Medien
    Digitale Medien
    Mathematical modeling of production of microbial lipids (1991)
    Springer
    Bioprocess and biosystems engineering 6 (1991), S. 227-234 
    Details
    ISSN: 1432-0797
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract As a part of the investigations on the microbial lipid production using the yeast Rhodotorula gracilis, CFR-1, kinetics of the biomass synthesis has been studied using shake flask experiments. Using a medium containing a carbon to nitrogen ratio of 70∶1, the rates of biomass production were followed at different initial substrate concentrations in the range of 20–100 kg/m3. A logistic model was found to be reasonably adequate to describe the kinetics of the growth of biomass; the maximum specific growth rate of 0.105 h−1 was applicable for substrate concentrations less than 60 kg/m3, which gave reasonable agreement between predicted and actual biomass concentration values.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00369716
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  • 14
    Digitale Medien
    Digitale Medien
    Gaseous concentration gradients in tray type solid state fermentors — Effect on yields and productivities (1992)
    Springer
    Bioprocess and biosystems engineering 8 (1992), S. 67-72 
    Details
    ISSN: 1432-0797
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract In aerobic solid state fermentation systems, interaction of mass transfer effects with bioreaction plays an important role on the yields and productivities of the bioreactors. Experimental observations on the oxygen and carbon dioxide concentration gradients in a tray type solid state fermentation system are reported in this paper. Steep gradients are experienced in deep beds making large portions of the bioreactor ineffective. The results are useful in the design of the bioreactor in terms of efficient mass transfer as well as critical thickness of the substrate bed to be used.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00369266
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  • 15
    Digitale Medien
    Digitale Medien
    Mathematical modeling of production of microbial lipids (1991)
    Springer
    Bioprocess and biosystems engineering 6 (1991), S. 241-248 
    Details
    ISSN: 1432-0797
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract In the microbial lipid production system using the yeast Rhodotorula gracilis, CFR-1, kinetics of lipid accumulation and substrate utilisation at initial substrate concentrations in the range of 20–100 kg/m3 were investigated using shake flask experiments. A mathematical representation based on logistic model for biomass and Luedeking-Piret model for lipid accumulation gave reasonably good agreement between the theoretical and experimental values for substrate concentration less than 60 kg/m3. The kinetic expressions and parameters obtained through shake flask studies were directly applied to experiments in the laboratory fermentors also and the models were found to hold good for the prediction of the change of biomass, product as well as substrate with time. The attainment of a saturation in the intracellular lipid accumulation with time, however, was not predicted by the model which was shown to be an inherent feature of the Luedeking-Piret model.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00369553
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  • 16
    Digitale Medien
    Digitale Medien
    A mathematical model for solid state fermentation in tray bioreactors (1993)
    Springer
    Bioprocess and biosystems engineering 8 (1993), S. 255-262 
    Details
    ISSN: 1432-0797
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Abstract A simple mathematical model for the interaction of mass transport with biochemical reaction in solid state fermentations (SSF) in static tray type bioreactors under isothermal conditions has been developed. The analysis has enabled scientific explanations to a number of practical observations, through the concept of critical substrate bed thickness. The model will be most useful in the prediction of the concentration gradients as also in efficient design of these bioreactors.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00369838
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  • 17
    Digitale Medien
    Digitale Medien
    Diffusional influences on the parametric sensitivity of immobilized enzyme catalystsNCL Communication No. 2141. (1978)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 20 (1978), S. 1817-1831 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Immobilization of an enzyme within an insoluble material permeable to substrate can change the apparent behavior of the enzyme. In particular, external mass transfer and intraparticle diffusion effects can significantly influence the dependence of observed reaction rate on operating parameters such as temperature and pH. This analysis shows that, under very general conditions, the influence of diffusion alone is to diminish the sensitivity of the observed rate to any parameter that is uniform throughout the catalyst particle. However, the optimal value of the parameter is not changed because of intrapellet diffusional effects.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260201110
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  • 18
    Digitale Medien
    Digitale Medien
    A useful sampling device for small fermentors with thick mycelial suspensionsNCL Communication No. 3084. (1983)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 25 (1983), S. 1677-1678 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260250622
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  • 19
    Digitale Medien
    Digitale Medien
    Studies on cellulase production by a mutant - penicillium funiculosum uv-49 (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 26 (1984), S. 1079-1084 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: In search of hypercellulolytic microorganisms, ultraviolet irradiation carried out with Penicillium funiculosum has yielded a superior mutant. The investigations reported in this article are shake flask studies on some important nutritional requirements of the mutant, namely, nitrogen source, carbon source, and inducers. The mutant shows an ability to metabolize inorganic nitrogen sources like urea and sodium nitrate both for growth and enzyme production. A comparison of the long-term saccharification ability and the utilization efficiency of the mutant enzyme with those reported in the literature is also carried out, showing the superior performance of the mutant enzyme.
    Zusätzliches Material: 9 Tab.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260260910
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  • 20
    Digitale Medien
    Digitale Medien
    Glycerol production by fermentation: A fed-batch approachNCL Communication No. 3955 (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 30 (1987), S. 325-328 
    Details
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bit.260300225
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