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  • 1
    Digitale Medien
    Digitale Medien
    Chromosomal localization of ceruoplasmin and transferrin genes in laboratory rats, mice and in man by hybridization with specific DNA probes (1987)
    Springer
    Chromosoma 96 (1987), S. 60-66 
    Details
    ISSN: 1432-0886
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Fragments of the natural rat ceruloplasmin (Cp) gene and cDNA copies of rat Cp and transferring (Tf) mRNAs highly labelled by nick translation with 125I-dCTP were used as specific probes for assignment of these genes to the metaphase chromosomes of rat, mouse and man by in situ hybridization. Both Cp and Tf genes were found to be syntenic in rodents, occupying with high probability the regions 9D and 9F1–3 in mice and 7q11–13 and 7q31–34 in rats respectively. The significant increase in silver grain count over chromosome 15 in rats after hybridization with both the Cp and Tf probes suggests the presence of a related pseudogene cluster on this particular chromosome and thus favours its partial homeology to chromosome 7. The localization of silver grains in metaphase chromosome of man indicates subregional assignment of the Tf gene to 3q21. Use of the rat Cp DNA probe does not indicate synteny of the Cp and Tf genes in man and suggests the existence of a related DNA sequence in 15q11–13. The potential and limitations of the in situ hybridization technique with heterologous DNA probes for gene mapping in mammalian species are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00285885
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  • 2
    Digitale Medien
    Digitale Medien
    De novo mutation within the intron-exon junction in the PiZ allele of the alpha-1-antitrypsin gene (1992)
    Springer
    Human genetics 〈Berlin〉 90 (1992), S. 169-170 
    Details
    ISSN: 1432-1203
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary A proband homozygous for the PiZ allele of the alpha-1-antitrypsin gene was found to be a heterozygous carrier of the additional nucleotide substitution (C-T) within the intron IV-exon V junction (position 9955 in intron IV, 3bp upstream of its 3′-splice site). This mutation was not found in DNA from either the PiZ heterozygous parents or the PiZ homozygous brother of proband.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00210767
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  • 3
    Digitale Medien
    Digitale Medien
    The action of x-rays on the motor function and the contractile proteins of motile cells (1959)
    Springer
    Bulletin of experimental biology and medicine 48 (1959), S. 1483-1486 
    Details
    ISSN: 1573-8221
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Experiments showed that there are no significant changes of the motor function in the seminal cells after x-ray irradiation of frogs (20, 000 r). This shows that disturbance of oxidative phosphorylation under the action of radiation is not a general biological regularity. The effect of ionizing radiation on the resynthesis of highenergy (ATP) differs in the cells of radiosensitive and radioresistant tissues; evidently it is primarily determined by the different localization of the corresponding enzyme systems therein. There are no visible disturbances in the ability of “glycerol models” of myofibrils, the cilia of the ciliated epithelium and spermatozoa (obtained from the tissues of irradiated animals), to interact with ATP. This points to a rather low radiosensitivity of the contractile cellular proteins and explains the high radioresistance in some phases of cell division connected with the separation of the chromosomes and equatorial division of the cytoplasm.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00792740
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  • 4
    Digitale Medien
    Digitale Medien
    Mapping apolipoprotein A-I and ceruloplasmin genes on human and murine chromosomes by in situ hybridization with specific human DNA probes (1988)
    Springer
    Bulletin of experimental biology and medicine 105 (1988), S. 703-706 
    Details
    ISSN: 1573-8221
    Schlagwort(e): genes ; DNA probes ; hybridization ; ceruloplasmin ; apolipoprotein A-I
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00841538
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  • 5
    Digitale Medien
    Digitale Medien
    On the defect of synthesis ceruloplasmin in the liver polyribosomes in Wilson's disease (1975)
    Springer
    Biochemical genetics 13 (1975), S. 533-550 
    Details
    ISSN: 1573-4927
    Schlagwort(e): Wilson's disease ; ceruloplasmin ; genetic defect in protein synthesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Comparative immunochemical analysis of ceruloplasmin-synthesizing polyribosomes in liver biopsies from control subjects and homozygous carriers of the Wilson's mutation was performed. According to I 125 -antibody binding data, the amount of ceruloplasmin-forming liver polysomes in patients with Wilson's disease was 10–20 times lower than that in non-Wilson patients. Correspondingly, the pulse labeling of ceruloplasmin polypeptides was decreased severalfold in the cell-free liver preparations from patients with Wilson's disease.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00484913
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  • 6
    Digitale Medien
    Digitale Medien
    Highly purified ceruloplasmin messenger RNA from rat liver (1981)
    Springer
    Molecular and cellular biochemistry 35 (1981), S. 171-182 
    Details
    ISSN: 1573-4919
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Summary Highly purified ceruloplasmin mRNA was isolated from rat liver polyribosomes. The molecular weight of ceruloplasmin mRNA is in a range from 1.05 to 1.25 · 106 daltons which is large enough to code for a putative precursor of ceruloplasmin (∼700 amino acids). Ceruloplasmin mRNA contains 3′-terminal poly(A) the length of which varies from 38 to 165 nucleotides. The 5′-end of ceruloplasmin mRNA is blocked with confronting m7G residue which is a component of cap I (m7G5′ppp5′XmpAp). The addition of ceruloplasmin mRNA to wheat-germ cell-free system programmed the synthesis of a product that was largely precipitated by anti-ceruloplasmin immunoglobulins. The translation product was homogeneous in polyacrylamide gel-sodium dodecylsulfate electrophoresis. Cell-free translation of ceruloplasmin mRNA was sensitive to inhibition by cap analogue.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02357087
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  • 7
    Digitale Medien
    Digitale Medien
    Polyribosomes and messenger RNA from RAT liver mitochondria (1977)
    Springer
    Molecular and cellular biochemistry 14 (1977), S. 101-108 
    Details
    ISSN: 1573-4919
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Summary Rat liver mitochondrial polyribosomes were isolated free from cytoplasmic ribonucleoprotein contaminations in a number of criteria (sedimentation and buoyant density patterns, ribosomal RNA composition). Heterogeneous poly A containing RNA from mitochondrial polysomes was purified by two-stage cellulose chromatography. This RNA was in vitro labelled with125I up to specific activity ~106–107 cts.min−1.µg −1 and used for hybridization experiments with separate complementary strands of mitochondrial DNA and nuclear DNA fragments. The proportions of mitochondrial poly A containing RNA that is complementary to heavy and light strands of mtDNA were respectively 31.5% and 8.3%. Besides, a significant RNA fraction was complementary to unique sequences of nuclear DNA (2–3 copies per haploid genome). The hybrids that were formed possessed a high Tm indicative of a perfect base pairing. A dual intracellular origin of mitochondrial messenger RNA is discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01734172
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  • 8
    Digitale Medien
    Digitale Medien
    On the cell-free system of protein synthesis from mammalian mitochondria (1977)
    Springer
    Molecular and cellular biochemistry 14 (1977), S. 91-96 
    Details
    ISSN: 1573-4919
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Summary Some properties of a submitochondrial cell-free system for protein synthesis are described. The system was prepared from rat liver mitochondria lysed with Triton X-100, and the lysate was characterized by a linear rate of [14C]amino acid incorporation for 15–20 min with subsequent decline in activity. The incorporation reaction was inhibited by chloramphenicol and was in-sensitive to cycloheximide. Poly(U) addition stimulated [14C]phenylalanine incorporation by the preincubated submitochondrial system. Upon the addition of 7.5S mRNA that was iso-lated from mitochondria the major translation product was identified as a hydrophobic poly-peptide which in some properties (solubility in chloroform-methanol mixture) was similar to one of polypeptides synthesized by the sub-mitochondrial system on endogeneous mRNAs.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01734170
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  • 9
    Digitale Medien
    Digitale Medien
    Isolation and partial purification of ceruloplasmin messenger RNA from rat liver (1977)
    Springer
    Molecular biology reports 3 (1977), S. 235-242 
    Details
    ISSN: 1573-4978
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Partially purified ceruloplasmin mRNA was isolated using indirect immunoprecipitation of rat liver polysomes and poly(U)-Sepharose chromatography of polysomal RNA. This RNA programmed the synthesis of ceruloplasmin polypeptides in a cell-free system from mitochondria. Immunochemical analysis of the translation products revealed a 40-fold enrichment of the ceruloplasmin mRNA activity. The purified ceruloplasmin mRNA migrated as a major homogeneous component with an apparent molecular weight about 1×106 daltons in polyacrylamide gels containing sodium dodecyl sulfate. The immunoprecipitated products of the cell-free translation had molecular weights in the range 4.5–5.4×104 daltons as estimated by gel-electrophoresis under denaturating conditions. These values approach the weight of the half-molecule of native ceruloplasmin.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00643479
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  • 10
    Digitale Medien
    Digitale Medien
    Identification of ceruloplasmin messenger RNA sequences in heterogeneous nuclear RNA from rat liver (1981)
    Springer
    Molecular biology reports 8 (1981), S. 57-62 
    Details
    ISSN: 1573-4978
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The distribution of the sequences of ceruloplasmin mRNA in different fractions of heterogeneous nuclear RNA from rat liver was studied using cDNA transcripts of highly purified mRNA as hybridization probe. The content of ceruloplasmin mRNA sequences in poly(A)-containing and poly(A)-free subfractions of heterogeneous nuclear RNA is respectively 1 and 27 molecules per a hepatocyte. Heterogeneous nuclear RNA carrying the sequences of ceruloplasmin mRNA sedimented in sucrose gradients containing formamide, as a broad zone around the 56S peak. Denaturing electrophoresis followed by the transfer of RNA onto diabenzyloxymethyl paper and hybridization with [32P]-cDNA revealed multiple high molecular weight fractions of ceruloplasmin pre=mRNA (9.0, 6.6, 2.2 and 1.6 megadaltons) in the non-adenylated fraction of nuclear RNA and a single 1.1–1.2 megadalton zone in poly(A)-containing nuclear RNA, the latter being equal in size to the mature ceruloplasmin mRNA from liver polysomes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00798386
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