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  • 1
    Electronic Resource
    Electronic Resource
    Surface-induced alkyl migration reactions in metal carbonyls (1980)
    s.l. : American Chemical Society
    Journal of the American Chemical Society 102 (1980), S. 5112-5114 
    Details
    ISSN: 1520-5126
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ja00535a057
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  • 2
    Electronic Resource
    Electronic Resource
    Secretion of MPB64 Antigen by a Recominant Clone of Mycobacterium Smegmatis: Characterization and Application for the Diagnosis of Tuberculosis (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 42 (1995), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: MPB64, a specific antigen to Mycobaeterium tuberculosis complex (TB complex), was produced and secreted by a clone of M. smegmatis-MPB64 where the structural gene of MPB64 was inserted using a new mycobacteria, E. coli shuttle plasmid pNIS vector. Antibodies against the recombinant MPB64 (rMPB64) were used for the reverse particle latex agglutination (RPLA) test to detect the MPB64 antigen rapidly. RPLA tests were applied to the stock cultures and the clinical isolates of mycobacteria to identify TB complex. RPLA with anti-MPB64 antibody-coated latex beads completely distinguished TB complex from other mycobacteria. Thus, it is suggested that RPLA with anti-MPB64 antibody would be a new, easy and inexpensive method for the rapid diagnosis of tuberculosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1995.tb03684.x
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  • 3
    Electronic Resource
    Electronic Resource
    Mutagenic Activity of Heat-Induced Cholesterol-Degradation Products (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 53 (1988), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Mutagenic activities of degradation products from pure crystalline cholesterol by heat-treatment in air were examined with a streptomycin-dependent strain from the Salmonella typhimurium TA98. Heatings at 225°C for periods of over 5 hr or at temperatures above 150°C for 7 hr were required for mutagens to be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1988.tb07877.x
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  • 4
    Electronic Resource
    Electronic Resource
    Degradation of Cholesterol in Egg Yolk by Rhodococcus equi No. 23 (1988)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 53 (1988), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The degradation of egg yolk cholesterol was investigated with Rhodococcus equi No. 23, a strain previously isolated from butter. R. equi No. 23 was inoculated into culture media (10 mL) containing egg yolk at various levels (0.2 to 1.4g) and grown with continuous shaking at 37°C for varying time periods. Egg yolk cholesterol was degraded via 4-cholesten-3-one into nonsteroid compounds with almost no accumulation of steroid intermediates, as detected by thin-layer chromatography.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1988.tb07782.x
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  • 5
    Electronic Resource
    Electronic Resource
    Combined Effect of pH and Sodium Chloride on the Heat-induced Aggregation of Whole Egg Proteins (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 51 (1986), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Heat-induced aggregation of whole egg proteins through various treatment combinations ranging from 70–85°C, pH 2.0–9.0, and NaCl concentrations of 0–3%, was investigated using multiple regression analysis and vertical flat-sheet polyacrylamide gel electrophoresis. The importance of single variable was in the order of temperature, pH and NaCl concentration, and the combined variable of pH and NaCl had a highly significant (p〈0.001) effect on soluble protein content. From the fractional and step-wise aggregation appearances of whole egg proteins in the electrophoretic patterns, the heat stability of main proteins was considered to increase as pH (pH〉5) and NaCl concentration increased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1986.tb13060.x
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of Heat Treatment on Preparation of Colorless Globin from Bovine Hemoglobin Using Soluble Carboxymethyl Cellulose (1986)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 51 (1986), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Optimum conditions for the preparation of colorless globin using soluble carboxymethyl cellulose (CMC) were established by mapping super-sim plex optimization. Response for minimization was calculated using two parameters of heme content and protein recovery. When three factors (initial pH, CMC concentration, and final pH) were used, the minimum response of 15.4 was obtained. However, much lower minimum response (8.6 or 6.2) was obtained by adding urea or by heating in addition to the above three factors. By determining the effects of heating temperature (20–80°C) at different final pH (2.25–3.06), the heme content of globin obtained from heated hemoglobin was remarkably lower (2.65.8%) than from unheated hemoglobin (29.2–70.7%).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1986.tb13933.x
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  • 7
    Electronic Resource
    Electronic Resource
    Serological studies of Porphyromonas (Bacteroides) gingivalis and correlation with enzyme activity (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 26 (1991), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Porphyromonas gingivalis from the human oral cavity was serologically characterized using absorbed and unabsorbed rabbit antisera. The reference strains were ATCC 33277, W50, W83, 381 and hara 1. The 432 isolates were from periodontal pockets of 63 patients with adult periodontitis. Using sonicated antigens, four serotypes were identified by immunodiffusion tests and immunoelectrophoresis. Each patient harbored only one serotype of P. gingivalis, and serotypes I and IV predominated. The incidence of serotype I was four times higher than that of serotype II, and approximately seven times higher than that of serotype III. The collagenolytic and some proteolytic enzymes of representatives of each serotype were assessed. Although all strains produced these enzymes to some degree, some differences in their levels were observed. Serotype II strains were more collagenolytic than serotypes I or III, and serotype III exhibited lower activities of N-CBz-glycyl-glycyl-arginyl peptidase than other serotypes. Antibiotic sensitivity was also compared with antimicrobial disks, and serotype IV strains exhibited high sensitivity to the four antibiotics used.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1991.tb01644.x
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  • 8
    Electronic Resource
    Electronic Resource
    Thiol-dependent collagenolytic activity in culture media of Bacteroides gingivalis (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 19 (1984), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Twenty strains of anaerobic bacteria including some oral species from Bacteroides, Eikenella, Fusobacterium, Capnocytophaga, and Actinobacillus were used for the detection of collagenolytic activity in their culture media (supernatants). Organisms were essentially grown in complete anaerobic basal broth, and collagenolytic activities in the culture media were measured using 14C-acetylated soluble collagen from rat skin. When the enzyme reaction was carried out in the standard reaction mixture for 60 min, very low collagenolytic activities were found in culture media of Bacteroides gingivalis 381, 1021, Bacteroides intermedius 163, K l–8, Bacteroides asaccharolyticus ATCC 27067, and Fusobacterium nucleatum ATCC 25586. However, the activity in the media of all species of B. gingivalis tested was enormously enhanced by the addition of reducing reagents having thiol-groups such as dithiothreitol, L-cysteine, etc. This stimulated activity was nearly completely inhibited by some thiol-blocking reagents. In contrast, these reagents had no effect on the collagenolytic activity in the media of the other anaerobes and in the collagenase preparation of Clostridium histolyticum. These results suggest that B. gingivalis produces thiol-dependent collagenolytic enzyme which may play an important role in the degradation of collagen fibrils in a reduced environment like the deep gingival pocket.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1984.tb01010.x
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  • 9
    Electronic Resource
    Electronic Resource
    Isolation and characterization of protease from culture supernatant of Bacteroides gingivalis (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 22 (1987), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Caseinolytic protease was isolated and purified, with high yield, from culture supernatant of Bacteroides gingivalis 381 by procedures including acetone fractionation, gel filtration on Sepharose CL-6B, solubilization with 0.8% 1-O-N-octyl-β-D-glucopyranoside and affinity chromatography on arginine-Sepharose 4B. By the affinity chromatography, the protease was separated into three isoenzymes, one of which was unadsorbed on the arginine-Sepharose 4B, but the other two of which were adsorbed and eluted with the buffer at a different concentration of arginine. Caseinolytic activities of the latter two were highly dependent on dithiothreitol and were inhibited by both thiol-blocking reagents and some microbial protease inhibitors; but such inhibition was not found in the former isoenzyme. The higher the affinity of the enzyme to arginine-Sepharose, the stronger the thiol dependency and the inhibition by these inhibitors. Phosphoramidone and ethylenediamine tetraacetate had no effect on the activity of any of the three peaks, indicating that the enzyme is not a metaloprotease. Besides the enzymehydrolyzed synthetic substrates of trypsin, such as BApNA, TAME, substrates of chymotrypsin and kallikrein were also hydrolyzed. The salivary and egg-white lysozymes were also degraded by this enzyme. These findings suggest that the protease produced by B. gingivalis may play a role as a periodontopathogen not only in directly destroying periodontal tissues but also in weakening the oral antibacterial mechanism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1987.tb02060.x
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  • 10
    Electronic Resource
    Electronic Resource
    Testicular Dynamics in Syrian Hamsters Exposed to Both Short Photoperiod and Low Ambient Temperature (2005)
    Berlin, Germany : Blackwell Verlag GmbH
    Anatomia, histologia, embryologia 34 (2005), S. 0 
    Details
    ISSN: 1439-0264
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The object of this study was to determine the details of morphological dynamics of spermatogenesis in Syrian hamsters exposed to both short photoperiod and low ambient temperature. Eight-week-old male hamsters, kept in a long photoperiod (14 h L, 10 h D), were transferred to a short photoperiod (6 h L, 18 h D) and kept there for 13 weeks to induce testicular regression. Some hamsters were then transferred from the room at 23°C to that at 5°C (5°C group). Remaining hamsters were continuously kept at 23°C (23°C group). Thereafter, the morphology was examined. As a result, it took only 8 weeks until spermatogenesis recovered in the 23°C group. However, it was not until 20 weeks that spermatogenesis was recognized in the 5°C group. As the regulation of seasonal testicular activity is characterized by coordinated shifts in the relationships among mitosis, meiosis, and apoptosis, the changes in the proliferative and apoptotic activities were examined. Although no significant difference in proliferative activity of spermatogonia between the 5°C and the 23°C groups was confirmed, a notable increase in the rate of apoptosis was observed in the 5°C group. Furthermore, this increase was more salient during the hibernation period. These findings suggest that both cold ambient temperature and hibernation caused the delay of testicular recrudescence and this delay arose from the increase of apoptotic activity but not the change in proliferative activity in spermatogonia in the 5°C group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0264.2005.00599.x
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