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  • Electronic Resource  (195)
  • 2000-2004
  • 1985-1989  (195)
  • 1920-1924
  • 1986  (195)
  • Life Sciences  (107)
  • Genetics
  • 1
    Electronic Resource
    Electronic Resource
    HLA-DR4 as a predictor of outcome three years after onset of rheumatoid arthritis (1986)
    Springer
    Rheumatology international 6 (1986), S. 233-235 
    Details
    ISSN: 1437-160X
    Keywords: Rheumatoid arthritis ; Genetics ; Natural history ; HLA seropositivity ; Disability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Data from 59 patients with early rheumatoid arthritis were analysed to determine the predictive value of the possession of HLA-DR4 for disease severity and functional outcome at 3 years after disease onset. The previously reported association of that antigen with seropositive disease was confirmed. Conversely, however, there was no evidence that functional outcome was worse in the HLA-DR4 positive group as measured by the Stanford Health Assessment Questionnaire. The mean articular index was lower in the HLA-DR4 group which also had a smaller proportion with limited wrist extension. In addition, both patient and physicians' global assessment of disease status were better in the HLA-DR4 group. It is concluded that HLA-DR4 is not a useful predictor of poor outcome at 3 years.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00541373
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  • 2
    Electronic Resource
    Electronic Resource
    Pelizaeus-Merzbacher disease in a brother and sister (1986)
    Springer
    Acta neuropathologica 69 (1986), S. 343-346 
    Details
    ISSN: 1432-0533
    Keywords: Leukodystrophy ; Demyelination ; Pelizaeus-Merzbacher disease ; Genetics ; Neurochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A brother and sister developed a slowly progressive neurological disorder with cerebellar and pyramidal signs and mild dementia. The brother developed symptoms at 6 months and died aged 11 years; the sister developed symptoms at 3 years and died aged 18 years. At post-mortem both had severe widespread central nervous system demyelination with islands of preserved myelin, and small amounts of sudanophilic lipid products. Metachromatic material, globoid cells, and adrenal abnormalities were not seen. The features were those of Pelizaeus-Merzbacher disease (PMD). It has been proposed, on the basis of only a few family studies, that PMD is an X-linked recessive disorder. These cases suggest that autosomal recessive inheritance may occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688316
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  • 3
    Electronic Resource
    Electronic Resource
    Glucose tolerance in siblings of Type 1 diabetic patients relationship to HLA status (1986)
    Springer
    Diabetologia 29 (1986), S. 39-45 
    Details
    ISSN: 1432-0428
    Keywords: Type 1 diabetes ; Genetics ; Aetiology ; Glucose tolerance ; HLA type ; Islet cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In this report, we present an analysis of glucose and insulin responses during oral glucose tolerance tests in 369 siblings of Type 1 diabetic patients. All have been HLA typed at the A, B and C loci. Though most had normal glucose tolerance by National Diabetes Data Group criteria (92% of the males and 95% of the females), siblings who shared both HLA haplotypes with the diabetic patient in the family had higher mean 3-hour glucose areas than those who shared one or neither HLA haplotype (p 〈 0.01). This difference was more marked in males and older siblings. Insulin concentrations did not differ significantly between the two groups except that, for those aged 〈16 years, the group sharing both haplotypes had lower fasting insulin concentrations (p = 0.05); for 16–29 year olds, the corresponding group had marginally higher 3-hour insulin areas than the remainder of siblings (p = 0.17). Little association with specific haplotypes (A1B8 or A2B15) was seen. Multivariate analyses, adjusting for age and obesity, eliminated the 3-h glucose difference in females by HLA sharing status (p = 0.37) although in males it remained significant (p 〈 0.001). Failure to account for age, sex and obesity may explain some of the conflicts in the reported literature. The glucose tolerance differences seen by HLA haplotype sharing status did not correlate with the presence of anti-islet cell antibodies. These results are consistent with the hypothesis that the HLA identical siblings, particularly males, have different (i. e. worse) glucose tolerance than their haplo-identical and non-HLA identical siblings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02427279
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  • 4
    Electronic Resource
    Electronic Resource
    The Dubowitz syndrome (1986)
    Springer
    European journal of pediatrics 144 (1986), S. 574-578 
    Details
    ISSN: 1432-1076
    Keywords: Dubowitz syndrome ; Genetics ; Growth retardation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The Dubowitz syndrome is a rare, autosomal, recessively inherited disorder of intrauterine and postnatal growth retardation leading to microcephaly, moderate mental retardation and such characteristic facial anomalies as telecanthus, epicanthic folds, blepharophimosis, ptosis, broadening of the bridge and tip of the nose, abnormal ears and retrogenia. Further findings include hyperactivity, eczema, cryptorchidism in the affected males, and brachy-clinodactyly of the fifth fingers. Thirty-three cases with this syndrome have been reported in the literature. Five additional patients are presented. All five are sporadic cases. The diagnostic symptoms and the differential diagnosis are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00496039
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  • 5
    Electronic Resource
    Electronic Resource
    The 4–5 Cycles per second rhythm — changes in time (1986)
    Springer
    European archives of psychiatry and clinical neuroscience 235 (1986), S. 299-300 
    Details
    ISSN: 1433-8491
    Keywords: 4–5 c/s rhythm ; Age ; Genetics ; EEG
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The 4-5 cycles per second (c/s) rhythm is a relatively rare, individual EEG variant. Age distribution of subjects carrying this variant and longitudinal studies over many years have indicated that it may sometimes disappear during middle age. Observations on female monozygotic twins at 15, 23 and 45 years of age suggest that disappearance of this trait might also be under genetic control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00515917
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  • 6
    Electronic Resource
    Electronic Resource
    Environmental effects on zein chromatograms of maize inbred lines revealed by reversed-phase high-performance liquid chromatography (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 607-612 
    Details
    ISSN: 1432-2242
    Keywords: Taxonomy ; Germplasm identification ; Varietal identity ; Environmental interaction ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Alcohol soluble seed storage proteins (zeins and alcohol soluble glutelins) of maize (Zea mays L.) were separated by reversed-phase high-performance liquid chromatography (RP-HPLC). The objectives were to assess the reproducibility of chromatographic profiles using seed of inbred lines that had been produced in different locations and years. Reproducible differences between sources were seen but these were restricted to proteins that contributed 2% or less to an inbred profile. The majority of variation (93% for peak percent area; 99.8% for elution time) was between inbreds. RP-HPLC can therefore provide distinctive phenotypic profiles that are largely characteristic of genotype. Such qualitative and quantitative data will be valuable for studies of taxonomy, evolution, genetics, and germplasm identification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264264
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  • 7
    Electronic Resource
    Electronic Resource
    Genetic analysis of gliadin-encoding genes reveals gene clusters as well as single remote genes (1986)
    Springer
    Theoretical and applied genetics 73 (1986), S. 278-285 
    Details
    ISSN: 1432-2242
    Keywords: Genetics ; Gliadins ; Gene clusters ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Analysis of F2 grains from two different crosses has revealed a complex organization of the family of gliadin-coding genes located on chromosomes of the first homoeological group in hexaploid wheat. Chromosome 1A of variety ‘Bezenchukskaya 98’ was found to carry at least five gliadin-coding genes of which three genes form a cluster controlling the synthesis of the GLD1A1 block. Two additional genes are located on the both sides of this cluster and recombine with it at frequencies of 5±1.3% and 13±2.9%. Gliadinencoding genes recombining with the main clusters were also found on chromosomes 1B and 1A in the ‘Bezenchukskaya 98’ and ‘Saratovskaya 210’ varieties, respectively. In ‘Chinese Spring’, widely used in genetic studies, we discovered a recombination between genes located on chromosome 1A and controlling the synthesis of ω- and γ-gliadins. Varieties and biotypes of one variety may differ by the presence or absence of such “selfish” (not included in clusters) gliadin components. The similarity of organization of prolamine-coding genes on chromosomes in different cereals is considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289286
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  • 8
    Electronic Resource
    Electronic Resource
    The pyridine nucleotide cycle of Salmonella typhimurium: Genetic characterization of the pncXA operon (1986)
    Springer
    Molecular genetics and genomics 205 (1986), S. 507-514 
    Details
    ISSN: 1617-4623
    Keywords: Pyridine nucleotide cycle ; NAD metabolism ; Salmonella typhimurium ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A series of Mud1 and Tn10 insertions were identified in the pncA chromosome region of Salmonella typhimurium which is responsible for the production of nicotinamide deamidase. Both pncA (resulting in no nicotinamide deamidase activity) and pncX (resulting in lowered nicotinamide deamidase activity) insertions were constructed. In addition, mutants which could utilize nicotinamide as a sole source of nitrogen were isolated. These mutants, designated pncH, hyperproduce nicotinamide deamidase. Genetic studies utilizing pncX-lacZ and pncA-lacZ operon fusions indicate that pncX::Tn10 insertions reduce transcription of pncA-lac while pncH mutations increase the expression of both pncA-lacZ and pncX-lacZ. The gene order was determined as purB-pncA-pncX-gdh with transcription of both pncA and pncX occurring in the counterclockwise direction. Merodiploid studies suggest a model whereby pncX and pncA form an operon with the major promoter occurring upstream from pncX. A second, weaker promoter for pncA must be situated between pncX and pncA. The pncH mutations appear to occur in the pncX promoter (pncXp) increasing promoter activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338090
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  • 9
    Electronic Resource
    Electronic Resource
    The genetics of actinorhizalFrankia: A review (1986)
    Springer
    Plant and soil 90 (1986), S. 429-453 
    Details
    ISSN: 1573-5036
    Keywords: Actinorhizae ; Frankia ; Genetics ; Nitrogen fixation ; Nodulation ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02277414
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  • 10
    Electronic Resource
    Electronic Resource
    Masthead (1986)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 7 (1986) 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020070201
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  • 11
    Electronic Resource
    Electronic Resource
    Isocitrate dehydrogenase in D. melanogaster imaginal discs: Pattern development and alteration by homoeotic mutant genes (1986)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 7 (1986), S. 21-34 
    Details
    ISSN: 0192-253X
    Keywords: Drosophila ; pattern formation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The distribution of the soluble form of NADP+ -dependent isocitrate dehydrogenase (ICDH) was examined in Drosophila melanogaster imaginal discs. Development of the enzyme patterns and the specific transformations of the patterns by homoeotic mutants were studied. ICDH pattern formation was followed in eye-antennal discs and wing discs from the late 2nd instar stage through 3rd instar and 8 hours into prepupal development. The patterns formed gradually in both disc types. The most interesting pattern developed in the eye portion of the eye-antennal disc complex. ICDH distribution as well as staining intensity correlated well with differentiation of the ommatidia. The spatial distribution of ICDH within the discs was under genetic control. The patterns reflected the state of determination of the disc. When the presumptive tissue type was transformed via mutant homoeotic genes to different determinative states, the ICDH pattern likewise transformed to the pattern characteristic of the newly acquired structure.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020070103
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  • 12
    Electronic Resource
    Electronic Resource
    Developmental profiles of three embryo-lethal maize mutants lacking leaf primordia: ptd*-1130, cp*-1418, and bno*-747B (1986)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 7 (1986), S. 35-49 
    Details
    ISSN: 0192-253X
    Keywords: zea mays ; embryogenesis ; defective kernel mutants ; genetic regulation ; maize ; leaf primordia ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The defective kernel (dek) mutants of maize are altered in both their embryo and endosperm development. Earlier studies have indicated that some of the dek mutants are unable to form shoot apical meristems or leaf primoirda. We have examined three embryo lethal dek mutants of this type, ptd*-1130, cp*-1418, and bno*-747B, to obtain a developmental profile for each. Allelism tests show that these three mutants are not allelic. Embryos were examined in early, mid-, and late kernel development as well as at kernel maturity by dissection and sectioning procedures and also at kernel maturity by scanning electron microscopy. All three mutants lag behind normal embryos in their rate of development. Embryos of ptd*-1130 reached the transition stage by early kernel development and progressed no further but underwent cell enlargement and necrosis during late kernel development. Embryos of cp*-1418 reached an early coleoptilar stage by midkernel development. They subsequently increased in size but did not form any leaf primordia. At kernel maturity, they no longer had a shoot apical meristem but often had a well formed root meristem. They appeared to remain healthy and did not become necrotic. Embryos of bno*747B reached the early coleoptilar stage by early kernel development but progressed no further. By kernel maturity, they had grown into masses of irregularly shaped embryonic tissue that no longer resembled any normal embryo stage but were not necrotic. None of these three mutants responded to attempts to support continued embryo development when cultured, but all three mutants formed callus on N6 and MS media supplemented with 2,4-D. These results indicate that these mutants are all uniformly blocked at specific stages early in embryonic development, have different subsequent developmental fates, and represent three different genes performing unique functions that are essential for embryogenesis.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020070104
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  • 13
    Electronic Resource
    Electronic Resource
    Noncoordinate synthesis of the vitellogenin proteins in tissues of Drosophila grimshawi (1986)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 7 (1986), S. 81-97 
    Details
    ISSN: 0192-253X
    Keywords: Hawaiian ; Drosophila grimshawi ; yolk proteins ; ovarian follicle development ; fat body ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In analyzing the in vitro pattern of protein synthesis by the fat body and ovaries of the Hawaiian species Drosophila grimshawi, we have found that the ovaries synthesize much more protein than the female fat body and that the majority of the synthesized proteins are retained by the ovarian tissues. In contrast, the fat body secretes most of the proteins into the culture medium. Vitellogenins are the major class of proteins synthesized and released into the medium by both tissues. The synthesis of the three vitellogenin proteins (V1, V2, V3,) is noncoordinate in the two tissues. Ovaries synthesize much more of the V2 protein, less V1 and very little V3, whereas fat body synthesies more Vs1 protein with lesser qu antities of the other two. The follicle cells were identified as the site of ovarian vitellogenin synthesis in D. grimshawi, confirming the findings in D. melanogaster. In D. grimshawi, the three vitellogenins are synthesized by the follicle cells in a noncoordinate and developmentally regulated manner. V2 and V1 are the predominant proteins at the onset of vitellogenesis (S8-9); their production peaks together with that of V3 a few hours later (S10) and then decreases to quantitiesequalto that of V3 during early choriogenesis (S11). During active choriogenesis (S12), V2 and V1 cease to be synthesized, but V3 synthesis continues. The vitellogenins synthesized by the follicles in vitro are released into the medium and not incorporated into the oocyte.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020070204
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  • 14
    Electronic Resource
    Electronic Resource
    Masthead (1986)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 7 (1986) 
    Details
    ISSN: 0192-253X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020070401
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  • 15
    Electronic Resource
    Electronic Resource
    Sex ratio and testis size in mice carrying Sxr and T(X;16)16H (1986)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 7 (1986), S. 177-185 
    Details
    ISSN: 0192-253X
    Keywords: sex reversal ; X-chromosome inactivation ; testis formation ; sex determination ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Mice heterozygous for the T(X;16)16H translocation and carrying Sxr on their normal (inactive) X chromosome (ie, T16H/X Sxr individuals) may develop as males, females, or hermaphrodites. The proportion of males varied from 22% to 65% depending on the source of the normal X chromosome. A model is proposed, according to which relatively small variations in the spreading of inactivation from the X chromosome into the attached Sxr fragment produce large changes in the proportion of males. Testis weight in T16H/X Sxr males was found to be significantly smaller than in X/X Sxr males, irrespective of the source of the normal X chromosome.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020070403
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  • 16
    Electronic Resource
    Electronic Resource
    The relationship of relative gene dose to the complex phenotype of the daughterless locus in Drosophila (1986)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 7 (1986), S. 205-221 
    Details
    ISSN: 0192-253X
    Keywords: gene dosage effects ; Drosophila sex determination ; maternal-zygotic gene interactions ; tandem duplications ; maternal effects ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The daughterless (da) gene provides an essential maternally supplied component for Drosophila sex determination and dosage compensation. In this connection, it is required as a positive regulator of a female-specific master regulatory gene, Sex-lethal (Sxl). In addition, zygotic da gene function is required for male and female viability. Thus, the phenotype da is complex; it includes both maternal and zygotic aspects, as well as both sex-specific and nonsex-specific aspects. Assessment of wild-type da function has relied on the characterization of only a single leaky mutant da allele. In order to better understand the nature of this allele and the relationships between the various aspects of its complex phenotype, tandem duplications of both the mutant and wild-type da alleles were isolated and used in a dose study of this gene's function. Three conclusions were reached: (1) by the most stringent genetic criteria, the mutant da allele is a simple hypomorph, an allele with reduced but non-zero levels of wild-type functions; (2) since increased dose of da+ had no effect on viability or progeny sex ratio, this gene seems not to be a dose-sensitive element of the X/A ratio sex determination signal; and (3) expression of the maternal da+ allele does make a contribution to the nonsex-specific developmental processes that require zygotic da+ function; however, that contribution is clearly minor. In contrast, the zygotic genotype with respect to da appears to have no effect on the expression of Sxl+ in the zygote, the sex-specific process that requires maternal da+ function.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020070406
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  • 17
    Electronic Resource
    Electronic Resource
    Abstracts, S261-S280 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S261 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020516
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  • 18
    Electronic Resource
    Electronic Resource
    Abstracts, S221-S240 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S221 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020514
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  • 19
    Electronic Resource
    Electronic Resource
    Abstracts, S281-S300 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S281 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020517
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  • 20
    Electronic Resource
    Electronic Resource
    Abstracts, S301-S320 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S301 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020518
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  • 21
    Electronic Resource
    Electronic Resource
    Abstracts, S321-S340 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S321 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020519
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  • 22
    Electronic Resource
    Electronic Resource
    Abstracts, S341-S360 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S341 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020520
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  • 23
    Electronic Resource
    Electronic Resource
    Abstracts, S361-S380 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S361 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020521
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  • 24
    Electronic Resource
    Electronic Resource
    Abstracts, S381-S400 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S381 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020522
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  • 25
    Electronic Resource
    Electronic Resource
    The utilization of starch by yeasts (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 109-115 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020206
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  • 26
    Electronic Resource
    Electronic Resource
    A comparative radiorespirometric study of glucose metabolism in yeasts (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 117-121 
    Details
    ISSN: 0749-503X
    Keywords: Hexose monophosphate pathway ; NADPH ; radiorespirometry ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: A comparative radiorespirometric study of glucos emetabolism in glucose-limited chemostat cultures of Saccharomyces cerevisiae, Candida utilis and Rhodosporidium toruloides was performed in an attempt to estimate the contribution of the hexose monophosphate (HMP) pathway to glucose metabolism. Radioactively labelled glucose was administered directly to the cultures in a constant substrate feed, without disturbance of the steady state. The 14CO2 yields from [1-14C]- and [6-14C]-glucose demonstrated that the HMP pathway activities for the three yeasts were very similar. Furthermore, a quantitative analysis of results indicated that the HMP pathway activities were close to the theoretical minimum needed to cover the NADPH requirement for biomass formation.
    Additional Material: 1 Tab.
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    Yeast group formed in India (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 141-142 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020210
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  • 28
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    Cloning and sequencing of the PHO80 gene and CEN15 of Saccharomyces cerevisiae (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 129-139 
    Details
    ISSN: 0749-503X
    Keywords: Saccharomyces cerevisiae ; regulation ; pho ; pho80 ; CEN15 ; nucleotide sequence ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The PHO80 gene, which is one of the regulatory genes exerting negative control in the pho system of Saccharomyces cerevisiae, was cloned. The 1·8 kb DNA fragment carrying the PHO80 gene was sequenced and one open reading frame large enough to encode 293 amino acids was found in the sequence. Northern blot analysis of poly(A)+-RNA isolated from cells grown under repressed and derepressed conditions revealed that (i) the size of the PHO80 message was around 1·4 kb, (ii) the expression of the PHO80 gene was not affected by the presence or absence or absence of inorganic phosphate in the medium, and (iii) the expression of the PHO80 gene was not affected by pho2, pho4, pho81, or by pho80 itself. a centromere sequence was found downstream of the PHO80 coding region.
    Additional Material: 8 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320020209
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  • 29
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    Third small meeting on yeast and energetics (SMYTE III) at Mook, the Netherlands, 21-23 August 1985 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 143-144 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020211
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  • 30
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    Linear DNA killer plasmids from the yeast Kluyveromyces (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 153-162 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020303
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  • 31
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    Ty, an endogenous retrovirus of yeast? (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 145-152 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020302
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  • 32
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    Mutations in ARS1 increase the rate of simple loss of plasmids in Saccharomyces cerevisiae (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 169-178 
    Details
    ISSN: 0749-503X
    Keywords: ARS1 ; plasmid maintenance ; replication ; segregation ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Autonomously replicating sequence (ARS) elements are DNA sequences that promote extrachromosomal maintenance of plasmids in yeast. Mutations generated in vitro in the ARS1 region were examined for their effect on plasmid maintenance in a yeast centromeric plasmid. Our data show that mutations in the regions surrounding the ARS1 consensus sequence cause increases in the frequency of simple loss (1:0) event without affecting the rate of non-disjunction (2:0). Removal of the consensus itself causes a drastic increase in the rate of simple loss. Sequences sensitive to mutagenesis were identified in each flanking region and differ with respect o their location and importance o ARS function. These results suggest that the role ARS1 plays in plasmid maintenance deals with the replication and/or localization of the plasmid in yeast.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320020305
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  • 33
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    Abstracts, S181-S200 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S181 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020512
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  • 34
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    Abstracts, S201-S220 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S201 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020513
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  • 35
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    Abstracts, S241-S260 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S241 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020515
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  • 36
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    Masthead (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986) 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020101
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  • 37
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    Degradation of organic nitrogen compounds by yeasts (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 1-34 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 11 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320020102
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  • 38
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    mRNA-dependent yeast cell-free translation systems: Theory and practice (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 35-52 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020103
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  • 39
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    Inter- and intra-species crosses between Candida albicans and Candida guilliermondii (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 53-58 
    Details
    ISSN: 0749-503X
    Keywords: Candida albicans ; Candida guilliermondii ; hybrid ; recombination ; segregants ; DNA ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Hybridization was shown to occur both between strains of the imperfect diploid yeast Candida albicans and between C. albicans and the distantly related hapoid yeast Candida (Pichia)guilliermondii Protorophic hybrids were selected from croses of multiply marked auxotropic mutants two species. In most cases, mild ultraviolet irradiation of the C. albicans partmer was reqiured.Examination of auxotropic markers in segregants from the hybrid indicated that recombination, rather than the heterokaryon, formation, had occured in these croses. The DNA content of the hybrids varied from diploid or aneuploid (for crosses between C. albicans and C. guilliermodii) to triploid (for C. albicabs × C. albicans). It seems possible that genetic exchange analogous to this may occur in nature.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320020104
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  • 40
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    Xth international specialized symposium on yeasts (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 69-73 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020106
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  • 41
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    Calendar (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 74-74 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020107
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  • 42
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    Identification of the RNA2 protein of Saccharomyces cerevisiae (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 59-67 
    Details
    ISSN: 0749-503X
    Keywords: Splicing ; S. cerevisiae ; RNA2 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The rna2-1 mutant Saccharomyces cerevisiae. has a consitional lethal phenotype, accumulating high molecular weight RNAs of intron-conataining nuclear genes at 36°C. The cloned RNA2 gene suppresses this phenotype and the RNA2 gene product has been implicated in RNA splicing. Rabbit antisera have been raised againts an N-terminal synthetic peptide taken from the RNA2 gene DNA sequence data, and against a β-galactosidase/RNA2 gene fusion protein. Both antisera identify that same 97-105 kd protein from S. cerevisiae cell extracts which is consistent with the predicted size of the RNA2 protein (from the 2800 nucleotide transcript size and DNA sequence data).
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320020105
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  • 43
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    Alcoholic fermentation by ‘non-fermentative’ yeastsCentraalbureau voor Schimmelcultures, Yeast Division, Delft, The NetherlandsDedicated to Dr Nelly J. W. Kreger-van Rij. (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 123-127 
    Details
    ISSN: 0749-503X
    Keywords: Yeasts ; fermentation ; ethanol ; Durham tube test ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: All type strains of ‘non-fermentative’ yeasts, available in the culture collection of the Centraalbureau voor Schimmelcultures, were reinvestigated for their capacity to ferment glucose in the classical Durham tube test. Although visible gas production was absent, nearly all strains produced significant amounts of ethanol under the test conditions. Under conditions of oxygen-limited growth, even strong alcoholic fermentation may occur in a number of yeasts hitherto considered as non-fermentative. Thus, shake-flask cultures of Hansenula nonfermentans and Candida silvae fermented more than half of the available sugar to ethanol. It is concluded that the taxonomic test for fermentation capacity, which relies on detection of gas formation in Durham tubes, is not reliable for a physiological classification of yeasts as fermentative and non-fermentative species.
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320020208
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  • 44
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    Masthead (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986) 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020301
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  • 45
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    Abstracts, S401-S420 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S401 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020523
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  • 46
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    Abstracts, S421-S440 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S421 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020524
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  • 47
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    Abstracts, S441-S442 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S441 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020525
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  • 48
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    Announcement (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 75-75 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020108
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  • 49
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    Masthead (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986) 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020201
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  • 50
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    A new type of methylamine oxidase: The sole oxidase produced during growth of Sporobolomyces albo-rubescens on primary alkylamines (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 87-92 
    Details
    ISSN: 0749-503X
    Keywords: Amine oxidase ; yeast ; methylamine ; n-butylamine ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Under conditions known to separate methylamine oxidase from benzylamine oxidase in other yeast strains, only a single oxidase could be detected in Sporobolomyces albo-rubescens. This occurred irrespective of whether methylamine or n-butylamine was the nitrogen source for growth. The oxidase did not attack benzylamine. It was concluded that this organism can only produce a methylamine oxidase. The enzyme was purified to 90% homogeneity and found to have properties significantly a methylamine oxidases previously characterised. It lost only 40% of its activity in 30 min at 45°C, whereas methylamine oxidase previously described had half-lives of from 2 to 9 min at 45°C. It showed also a lower activity with short chain 1-aminoalkanes and a higher activity with longer chain 1-aminoalkanes than other methylamine oxidases, and had a significantly smaller subunit molecular weight (57 000 compared with 80 000).
    Additional Material: 4 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320020203
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    Vanadium metabolism in wild type and respiratory-deficient strains of S. cerevisiae (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 77-85 
    Details
    ISSN: 0749-503X
    Keywords: Vanadium metabolism ; magnetic resonance spectroscopy ; respiratory-deficient strains ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Vanadium metabolism was studied in a wild type and respiratory-deficient strains of S. cerevisiae. Inhibition of growth by vanadate [V(+5)], vanadate accumulation, and conversion of medium vanadate [V(+5)] to both cell-associated and medium vanadyl [V(+4)] and vanadate [V(+5)] were compared. The growth of both the parental and respiratory-deficient strains was inhibited by vanadate at concentrations greater than or equal to 1 mM. Both parental and respiratory-deficient strains accumulated vanadate and converted medium vanadate to cellular vanadyl as detected using electron spin resonance (ESR). The accumulation of cell-associated vanadyl was correlated with the loss of medium vanadate in both strains using a chemical assay. In contrast, the respiratory-deficient strain showed a greater amount of a cell-associated vanadate compound, as detected with vanadium-51 nuclear magnetic resonance (51V-NMR), than the wild strain or a representative respiratory-competent vanadate-resistant mutant. These data imply that mitochondrial function may be directly involved in vanadium metabolism.
    Additional Material: 3 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320020202
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    Synergistic effects of weak-acid preservatives and pH on the growth of Zygosaccharomyces bailii (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 93-100 
    Details
    ISSN: 0749-503X
    Keywords: pH ; weak acid ; synergism ; growth ; Zygosaccharomyces ; yeast ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In completely randomised factorial experiments, individual and synergistic effects of pH, benzoic acid and sorbic acid on the growth rate of the yeast Zygosaccharomyes bailii were determined, and expressed in polynomial equations. Synergism between benzoic and sorbic acid was pH dependent. A distinct effect of the anionic form of benzoic acid on doubling time was demonstrated by experiments in which concentrations of benzoic acid and benzoate were varied. The resultant polynomial equation showed that both species act synergistically.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320020204
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  • 53
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    Mechanism of inactivation of UDP-glucose 4-epimerase from Saccharomyces cerevisiac by D-xylose and L-arabinose (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 101-108 
    Details
    ISSN: 0749-503X
    Keywords: L-Arabinose ; D-xylose ; UDP-glucose 4-epimerase ; inactivation ; Saccharomyces ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: In a previous paper (Cármenes et al., 1984) we reported that UDP-glucose 4-epimerase from Saccharomyces was inactivated both in vitro (Crude extracts) by L-arabinose or D-xylose. In this paper, we reported that pure epimerase requires the presence of UMP or UDP to be inactivated by sugars and that the inactivation is due to the reduction of the epimerase NAD+, which is essential for epimerase activity. The inactivation rate is directly proportional to epimerase and sugar concentrations and hyperbolically proportional to UMP concentration. In situ experiments made with permeabilized cells showed that epimerase is inactivated in the same way when it is inside the cell. In vivo studies showed that epimerase is inactivated to a smaller extent when 1% Dgalactose is present in the culture medium than when 1% ethanol is the main carbon source.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320020205
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  • 54
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    Yeast/E. coli shuttle vectors with multiple unique restriction sites (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 163-167 
    Details
    ISSN: 0749-503X
    Keywords: Shuttle vectors ; gene cloning ; Saccharomyces ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Two yeast/E. coli shuttle vectors have been constructed. The two vectors, YEp351 and YEp352, have the following properties: (1) they can replicate autonomuosly in Saccharomyces cerevisiae and in E. coli; (2) they contain the β-lactamase gene and confer ampicillin resistance to E. coli; (3) they contain the entire sequence of pUC18; (4) all ten restriction sites of the multiple cloning region of pUC18 including EcoRI, SacI, KpnI, SmaI, BamH1, XbaI, SbaI, SalI, PstI, SphI and HindIII are unique in YEp352; these sites are also unique in YEp351 except for EcoRI and KpnI, which occur twice; (5) recombinant plasmids with DNA inserts in the multiple cloning region of YEp351 and YEp352 can be recognised by loss of β-galactosidase function in appropriate E. coli hosts; (6) YEp351 and YEp352 contain the yeast LEU2 and URA3 genes, respectively, allowing for selection of these grown under non-selective conditions indicative of high plasmid copy number. The above properties make the shuttle vectors suitable for constructions of yeast genomic libraries and for cloning of DNA fragments defined by a large number of different restriction sites.The two vectors have been further modified by deletion of the sequences necessary for antunomous replication in yeast. The derivative plasmids YIp651 and YIp352 can therefore be used ti integrate specific sequences into yeast chromosomal DNA.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/yea.320020304
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    Physical separation and functional interaction of Kluyveromyces lactis and Saccharomyces cerevisiae ARS elements derived from killer plasmid DNA (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 179-191 
    Details
    ISSN: 0749-503X
    Keywords: ARS elements ; bifunctional vectors ; plasmids ; DNA replication ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Teo DNA fragments which have autonomously replicating sequence (ARS) activity in both Saccharomyces cerevisiae and Kluyveromyces lactis have been isolated from the K. lactis kl killer plasmid. One fragment (Kla1) is 700 base pairs (bp) in length plasmids carrying it are mitotically unstable in both hosts. In K. lactis, this instability leads to colonies having a ‘nibbled’ phenotype when grown on selective media and appears to be the results of inefficient plasmid segregation. The other fragment of 1100 bp which was produced during the cloning procedure. Kla2 has been divided into the sub-fragments Kla2A and Kla2B which have, respectively, ARS activity in K. lactis and S. Cerevisiar but not the other species. This indicates that these two closely elated yeasts have different sequence requrements for ARS activity. Kla2B contains a perfect match to the S. cerevisiae ARS consensus but Kla2A does not. Both Kla2A and Kla1 share a 10 bp sequence as the sole region of homology between them. This sequence, 5‘TCATAATATA3’, is tentatively offered as defining the ARS consensus sequence for K. lactis.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020306
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    Masthead (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986) 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020401
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  • 57
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    Orthogonal-field-alternation gel electrophoresis banding patterns of DNA from yeasts (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 193-204 
    Details
    ISSN: 0749-503X
    Keywords: Orthgonal-field-alternation gel electrophoresis ; karyotyping ; ascomycetous yeasts ; basidiomycetous yeasts ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Chromosomal DNAs from various yeast species were separated by orthogonal-field-alternation gel electrophoresis (OFAGE). To this end we developed a spheroplasting and lysis method to obtain intact DNA from both ascomycetous and basidiomycetous yeasts. The OFAGE banding patterns of 22 ascomycetous and four basidiomycetous yeast strains were compared. The strains represented species from the genera: Brettanomyces, Candida, Croococcus, Filobasidiella, Geotrichum, Hansenula, Kluyveromyces, Pachysolen, Pichia, Rhodosporidium, Rhodotorula, Saccharomyces, Saccharomycodes, Saccharomycopis, Schizosaccharomyces and Zygosaccharomyces. Variations occurred in the number of bands and their positions in the gel, not only among strains of different genera but also among species from the same genus and even between varieties of the same species. The ascomycetous yeasts, with the exeption of Saccharomyces cerevisiae, only showed one to five bands of DNA larger than 1000 kilobase pairs (kb) in general none smaller. The paterns of the four basidiomycetous yeasts revealed also a few large DNA bands but in addition one to six bands ranging in size from 500 to 1000 kb, with the exception of a single smaller chromosome in Rhodotorula mucilaginosa. From the OFAGE banding patterns of strains studied here it appears that in Saach. cerevisiae the partitioning of DNA over chromosomes in unique. But rather than the large number of chromosomes, the presence of four chromosomes with less than 500 kb of DNA is characteristic for Sacch. cerevisiae.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020307
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  • 58
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    Involvement of lipids in solute transport in yeasts (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 205-220 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020402
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  • 59
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    Misconceptions about the energy metabolism of Saccharomyces cerevisiae (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 221-228 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020403
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  • 60
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    Calendar (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 237-237 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020405
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  • 61
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    Masthead (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986) 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020501
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  • 62
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    Sequences important for gene expression in filamentous fungi (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. 229-236 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020404
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  • 63
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    Scientific program (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. i 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020502
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  • 64
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    Abstracts, S1-S20 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S1 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020503
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  • 65
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    Abstracts, S21-S40 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S21 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020504
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  • 66
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    Abstracts, S41-S60 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S41 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020505
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    Abstracts, S61-S80 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S61 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020506
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    Abstracts, S81-S100 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S81 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020507
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  • 69
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    Abstracts, S101-S120 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S101 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020508
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  • 70
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    Abstracts, S121-S140 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S121 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020509
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  • 71
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    Abstracts, S141-S160 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S141 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020510
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    Abstracts, S161-S180 (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 2 (1986), S. S161 
    Details
    ISSN: 0749-503X
    Keywords: Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320020511
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  • 73
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    Production, characterization and use of expendable kefir starter culture in the kefir production process (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 167-174 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Upon sterilization, freeze-dried kefir grains are to be supplemented with yeast preparation, yeast showing the lowest survival in the course of freeze drying.A mixture of 20% sucrose solution and starch appeared to be the most efficient dispersing agent to protect yeast in the freeze-drying process.A starter which is produced with the kefir starter culture, does not differ in its microbiological, biochemical and organoleptic properties from a starter as obtained with kefir grains in the same time, such starter shows a better consistency.The preparation of such starters is less laborious and less complicated than the preparation of starters using kefir grains.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060215
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  • 74
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    The effect of automatic ph maintenance on steady state cultivation regimes and their stability. A theoretical study of the case of a perfect ph controller and perfect stirring (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 175-187 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The dynamics of pH-controlled chemostat and pH-auxostat is considered. The medium flow governed by pH-controller is calculated using the ionic (mass-charge) balance equations. The generalized equations of pH-controlled continuous fermentation are developed for the case of two variables: cell biomass and residual substrate concentrations. The study of steady state solutions has shown a substantial dependence of the variable patterns on a concentration of alkali (or acid) in titrant medium flow. The generalized analytical expressions for eigenvalues (reciprocals of transient characteristic times) of the culture dynamics have been obtained. The rates of transients during the steady state establishment are considered using these eigenvalues.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060216
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  • 75
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    Flow visualization III. Proceedings of the third International Symposium on flow visualization, September 6-9, 1983, University of Michigan, Ann Arbor, Michigan, USA, Washington, New York, London: Hemisphere Publishing Corporation. Heidelberg, New York, Tokyo, Berlin: Springer-Verlag, 1985, 888 pp., 910 fig., 288 DM. (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 188-188 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060217
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  • 76
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    Microbial Adhesion and Aggregation. Dahlem Workshop Reports, Life Sciences Research Report 31, Berlin, Heidelberg, New York, Tokyo: Springer-Verlag, 1984. 423 S., 86 Abb., 35 Tab., 59 DM (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 200-200 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060219
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  • 77
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    Gentechnik - Einführung in Prinzipien und Methoden. Uni-Taschenbücher 1290, Stuttgart: Gustav Fischer Verlag, 1985. 362 S., 178 Abb., 33 Tab., 28,80 DM (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 205-205 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060222
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  • 78
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    Silierung durch aerobe Säuerung - paradoxer Einfall oder praktische lösung? (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 201-204 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: For ensiling of herbage which is rich in protein and therefore difficult to ferment a procedure is proposed. It is based on aerobic acidification using biological deoxydants. The crops are treated with a suspension of living cells of Acetobacter methanolicus and a water solution of ethanol. This treatment brings about environmental conditions which promote lactic acid fermentation: anaerobiosis, complex nutritional supply and protective concentrations of H+-ions. Preliminary attempts have confirmed the principle of conservation by aerobic acidification combined with biological deoxydation in agricultural practice. Damages of legume crops with high protein content are prevented. The advantages of the new ensiling process is discussed in comparison to traditional treatment.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060221
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  • 79
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    Handbook of neurochemistry. Vol. 2. Experimental Neurochemistry. Second Edition. New York, London: Plenum Press, 1982. 475 pp., $ 59.50 (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 205-205 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060223
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  • 80
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    Molekular- und Zellbiologie. Berlin, Heidelberg, New York, Tokyo: Springer-Verlag, 1985. 199 S., 32 DM (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 206-206 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060224
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  • 81
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    Masthead (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986) 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060301
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  • 82
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    Partikelelektrophoretische Charakterisierung der Oberflächeneigenschaften von alkanutilisierenden Hefezellen: Chemische Zusammensetzung und Tensidadsorption (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 221-231 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: By using particle electrophoresis and quantitative analysis the interaction of anionic surfactants (sodium dodecylculfate, sodium decylbenzene sulfonate) with a hydrocarbon-grown yeast and a commercial baker's yeast has been investigated to obtain further informations about the chemical composition of the surface region (3-6 nm) of the cell wall of this yeasts. A correlation is found between the chemical composition and the different adsorption behaviour of various batches of the same yeasts. It was found that surface-localized hydrophobic glycoproteins, probably proteophosphomannan are responsible for the strong pH dependent adsorption behaviour of a typical Candida species.In contrast the cell surface of a typical Saccharomyces species was strongly hydrophilic and showed no surfactant adsorption.This can be explained by the presence of a polysaccharide probably phosphomannan in the surface region only.The implication of proteins in the adsorption process is confirmed by model experiments too.The models for the yeast cell wall proposed by LAMPEN and KIDBY et al. were critically discussed in terms of our results.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060305
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  • 83
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    Clnsteranalyse und Diskriminanzanalyse. Stuttgart, New York: Gustav-Fischer-Verlag, 1985. 135 S., 44 DM (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 232-232 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060306
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  • 84
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    Microbiological solubilization of metals from complex sulfide ore material in aerated column reactors (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 233-238 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Finely ground ore material (90%-200 mesh) was subjected to acid microbiological leaching in 20% and 30% (wt/vol) suspensions. The rate of aeration was maintained at 2 and 4 1/min · 1. After the onset of the microbiological phase of leaching, the solubilization of iron was initially rapid but was succeeded by Fe(III) precipitation. Major differences were apparent between inoculated and uninoculated suspensions for each metal analyzed (Fe, Al, Co, Cu, Ni, Zn). The sulfur balance in leach residues indicated elevated levels of elemental S and sulfate in the inoculated systems.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060307
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  • 85
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    Inhibitory effects of ethanol in alcoholic fermentation (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 239-243 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The ethanol tolerance behaviour of the strain Saccharomyces cerevisiae Sc 5 regarding the growth is characterized by a threshold ethanol inhibitory concentration (P1' = 42.5 g/l) and a linear relationship between the specific growth rate and the ethanol concentration within the limits P1' 〈 P 〈 P′. The maximum ethanol concentration for growth amounts to P′ = 84 g/l.A general model for the inhibition of growth and alcohol production, respectively, caused by ethanol, is deduced from experimental and bibliographical data: \documentclass{article}\pagestyle{empty}\begin{document}$$\begin{array}{l} \mu = \mu_0 - {\rm a}'(P - {P_{1}}^{\prime})^{{\rm b}'}, \\ \nu = \nu_0 - {\rm a}(P - P_1 )^{\rm b}.\end{array}$$\end{document}If the inhibitory effects are linear, the exponents b, b' become 1.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060308
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  • 86
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    Lecture Notes in Biomathematics Vol. 67. Mathematics in Biology and Medieine Proceedings, Bari 1983. Berlin, Heidelberg, New York, Tokyo: Springer-Verlag, 1985. 524 S. (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 244-244 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060309
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  • 87
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    Discovery and Isolation of Microbial Products. Chichester: Ellis Horwood Limited, 1985, 198 S., 23.50 L. (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 272-272 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060315
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  • 88
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    Optimization of solid-state fermentation of citrus dried peel by aspergillus niger in a packed bed column (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 253-258 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The protein enrichment of citrus dried peel on solid-state fermentation by Aspergillus niger in packed bed column was optimized using a two factor central composit design with α = 2 considering the “initial moisture content of the fermentation mass” and the “temperature in the centre of the fermenter”. It results significance for the moisture content (optimum 56%) and non significance for the temperature in the conditions employed. This indicates that a considerable range of temperature increase for the investigated SSF system could be allowed.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060312
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  • 89
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    Immobilized glucose dehydrogenase for cofactor regeneration in heme-catalyzed demethylation and hydroxylation reactions (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 265-271 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Glucose dehydrogenase (E.C. 1.1.1.47) from B. megaterium M 1286 was immobilized together with mutarotase (E.C. 5.1.3.3) on several organic carriers and by different methods. The storage stability of the enzyme at pH-values 〉 6 is slightly improved by immobilization and the pH-optimum is shifted from 8.3 to 8.0. Kinetic constants of the immobilized enzyme are: K′M(NAD+) = 5.36 × 10-4 mol/l K′M(glucose) = 3.76 · 10-2 mol/l and V′max = 5.54 · 10-5 mol/(l min g carrier) for the most active preparation (2.16 mg enzyme/g carrier).In reactor experiments the immobilized glucose dehydrogenase was used with glucose to regenerate NADPH in NADPH-dependent iron-III-protoporphyrin-IX-imidazole catalyzed hydroxylation and demethylation of model substrates of cytochrome P-450.The advantages of the coupling of both reactions with cofactor recycling are shown and discussed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060314
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  • 90
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    Zur Gewinnung von Ubichinon-10 aus Acetobacter methanolicus IMET B 346 (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 277-279 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Mixtures of acetone/water (93 : 7 ··· 90 : 10, v/v) are favoured solvents for the extraction of ubiquinone-10 from Acetobacter methanolicus IMET B 346. Using these solvent mixtures ubiquinone-10 was extracted nearly completely. Other lipids were extracted partially. Extracts were obtained by optimal conditions with a ubiquinone content 〉 5%.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060317
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  • 91
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    Methods of Enzymatic Analysis (3rd Edition). Vol. VIII. Metabolites 3: Lipids, Amino Acids and Related Compounds. Weinheim: VCH Verlagsgesellschaft, 1985. 629 (654) S., 325 DM (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 280-280 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060318
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  • 92
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    Herstellung von L-Lysindecarboxylase aus Klebsiella pneumoniae (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 273-276 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: L-Lysine decarboxylase (LL-Lysine carboxylase E.C. 4.1.1.18) is produced by a strain of the genus Klebsiella.The bacterial cells are cultivated at a temperature of 37°C with and without shaking. The production of LL-Lysine decarboxylase is increased by adding of LL-Lysine to the medium. At the end of cultivation the cells are killed by thymol, washed in water and dried by means of acetone and ether.Compared with specimen derived from other bacteria the LL-Lysine decarboxylase in the shape of dried cells shows a high specific activity. The preparations are free of disturbing enzyme activities, have a high level of stability and may be used for determining of LL-Lysine by means of enzyme electrodes.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060316
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  • 93
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    Assimilation of animal fats by yeasts and synthesis of protein by continuous cultivation in a complex nutrient medium (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 281-285 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Under the conditions of continuous cultivation studies were carried out on a mixed culture (association) with two strains of yeasts: Candida sp., assimilating animal fats, and Candida scottii, assimilating reducing sugars. A complex nutrient medium of wood hydrolysate was used.It was established that in this medium, at a rate of dilution of D = 0.25 h-1, the mixed culture assimilates the fats and the sugars simultaneously. As a result of this the yield of absolutely dry yeasts with regard to the total sum of fats and sugars amounts to 71% in the case of incineration fat and to 82% in the case of technical pork fat.The contents of protein, amino acids, lipids and ashy substances in the yeasts obtained from a medium with fats do not differ substantially from those obtained from the same medium without fats.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060319
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  • 94
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    The Physiological Properties of Plant Protoplasts. Berlin, Heidelberg, New York, Tokyo: Springer Verlag, 1985. 283 S., 82 Abb., 35 Tab., 115 DM (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 286-286 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060320
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  • 95
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    Xylan-Containing supports used to separate xylanase from the cellulases of aspergillus terreus F-413 (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 153-160 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The separation of xylanase from cellulolytic enzymes of A. terreus F-413 by affinity chromatography on xylan-containing supports was investigated. Xylanase purified over tenfold was obtained after column chromatography on xylan bound to controlled porous glass. The molecular weight of the purified enzyme has been found to be 140 000 daltons, and it is homogeneous in polyacrylamide gel electrophoresis. Purified xylanase also showed residual celluloytic activity (perhaps cross-specificity) with cellulosic substrates.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060213
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  • 96
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    Renaissance der Oberflächenkultur: Produktion von Mutterkornalkaloiden und Sporenbildung (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 245-251 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Industrially acceptable production of ergot alkaloids in surface culture under sterile conditions is described. After elucidation of the biochemical and morphological differentiation in the production microorganisms it is possible to elaborate a relatively simple cultivation method with good yields. Surface cultivation is especially advantageous for the production of spores in large quantities which are used as inoculum in industrial fermentations or as infection material (field production of ergot alkaloids, mycoinsecticides).
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060310
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  • 97
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    Praxis der Sicherheitsanalysen in der Verfarrenstechnik DECHEMA-Monographien, Bd. 100. Vorträge vom 2. Sonderkolloqium der DECHEMA über Sicherheitstechnik am 6. März 1985. Weinheim, Deerfeld Beach/Florida, Basel: Verlag Chemie, 1985. 171 S. (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 252-252 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060311
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  • 98
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    Sorption von Cellulase an Weizenstroh und seine Komponenten (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 259-264 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: By comparing different activity data of the buffered cellulase solution before and after contact with the substrate the interaction between Penicillium janthinellum cellulase and wheat straw, resp. its components (holocellulose and isolated lignin) has been investigated. The loss of activity due to sorption or denaturation has been found to differ widely between the different activity data and between the various substrates. A remarkable loss of enzyme activity was observed after contact with isolated straw lignin. The differences in activity decrease between the cellulose and the lignin moiety were found to be largent with the cellobiase activity.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060313
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  • 99
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    Rekombinierte DNA - Eine Einführnng. Heidelberg: Spektrum-der-Wissenschaft-Verlagsgesellschaft, 1985. 223 S. Originaltitel: Recombinant DNA - A short course. Aus dem Amerikanischen ubersetzt von M. LUDWIG-FESTL (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 292-292 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060322
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  • 100
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    Hybridoma cells produced by electrofusion in a homogeneous electric field (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 287-291 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: In the customary technique will be used a fusion chamber with an inhomogeneous electric field. The disadvantage is that the fusion conditions are not constant in the chamber. Yet for di-electrophoretic collection of the cells their own field-inhomogenizing influence is sufficient that is, the electrofusion can proceed also in very simple chambers with homogeneous fields.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060321
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