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  • Agrobacterium
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  • 1
    ISSN: 1432-203X
    Keywords: Agrobacterium ; auxin biosynthesis genes ; barley and tobacco protoplasts ; transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Agrobacterium tumefaciens and some Agrobacterium rhizogenes strains possess auxin biosynthesis genes (tms and aux genes respectively), responsible for a de novo auxin biosynthetic pathway in transformed plant cells. A comparison is presented of the potential expression of these genes in a monocotyledonous (barley) and a dicotyledonous plant (tobacco). The promoters of the genes were translationally fused to the β-glucuronidase reporter gene and analysed in transient expression experiments. The tms and aux fusions were highly expressed in tobacco, but not in barley. However, the aux enhancer active in tobacco, conferred low β-glucuronidase expression in barley when fused to a truncated cauliflower mosaic virus 35S promoter. The results are discussed in relation to the differential responses to Agrobacterium infection in monocots and dicots.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 14 (1994), S. 59-64 
    ISSN: 1432-203X
    Keywords: Agrobacterium ; transformation ; T-DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three chrysanthemum (Dendranthema grandiflora) cultivars were cocultivated with 2 Agrobacterium tumefaciens strains in combination with 4 pBIN19 derived binary plasmids, all carrying the Nosnptll selection gene and 35Sgus(intron) reporter gene. All binary plasmids transferred DNA to chrysanthemum explants but only pMOG410 gave good stable expression of GUS. This plasmid differs from the other plasmids in 2 aspects: 1) It carries a restored nptll gene and 2) the selection gene is positioned at the left border side of the reporter gene. Cocultivation with AGLO(pMOG410) yielded up to 13 GUS positive shoots per 100 explants. The presence of the gus and nptll gene in recovered shoots was confirmed by PCR and Southern blot analysis.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 13 (1994), S. 145-148 
    ISSN: 1432-203X
    Keywords: muskmelon ; gene transfer ; Agrobacterium ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cotyledon explants of muskmelon (Cucumis melo L., cv. Amarillo Oro) seedlings were co-cultivated with disarmed Agrobacterium tumefaciens strain LBA4404 that contained the binary vector plasmid pBI121.1. The T-DNA region of this binary vector contains the Nopaline synthase/neomycin phosphotransferase II (NPTII) chimeric gene for kanamycin resistance and the Cauliflower Mosaic Virus 35S/β-glucuronidase (GUS) chimeric gene. After infection, the cotyledon pieces were placed in induction medium containing 100 mg/l kanamycin. Putative transformed shoots were obtained, followed by the development of morphologically normal plantlets. The transgenic nature of regenerants was demonstrated by polymerase chain reaction, Southern blot analysis, plant growth on medium selective for the transgene (NPTII) and expression of the co-transformed GUS gene. Factors affecting the transformation procedure are discussed.
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  • 4
    ISSN: 1432-203X
    Keywords: Medicago sativa ; Alfalfa ; Transformation ; Agrobacterium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The trait for somatic embryogenesis is being introduced sexually into alfalfa (Medicago sativa) breeding populations to facilitate genetic transformation of this crop. Cocultivation experiments were conducted with an agronomically-improved embryogenic clone from one such population as well as with two other embryogenic clones, one of which was the source of the embryogenic trait in the breeding populations. Transgenic plants were produced from the agronomically-improved clone whereas none were produced from the other two clones. Among the 16 transgenic plants analyzed there was a range in both copy number and number of integration sites for the NPT-II gene; those plants regenerated after a prolonged selection phase in vitro generally had the highest numbers in both respects. There was no evidence of sectoral chimerism of the transgene in a subsample of transgenic plants analyzed by PCR.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 161 (1994), S. 300-309 
    ISSN: 1432-072X
    Keywords: Agrobacterium ; 16S rDNA ; 16S rDNA-23S rDNA Intergenic spacer ; tmr Gene ; nos Gene ; virA Gene ; virB2 Gene ; Polymerase chain reaction ; Restriction fragment length polymorphism ; Crown gall
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chromosomes and Ti plasmids of 41 Agrobacterium strains, belonging to biovars 1, 2, 3, and Agrobacterium rubi species were characterized by the restriction fragment length polymorphism of PCR-amplified DNAs. Profiles that were obtained by the analysis of the amplified 16S rDNA confirmed the grouping of the strains according to their species. Higher polymorphism was detected in the intergenic spacer between the 16S rDNA and 23S rDNA genes, allowing efficient discrimination of strains. Identification of most strains was possible, and the genetic relatednesses of Agrobacterium strains could be estimated. The analysis of the plasmid Ti encoded regions between the tmr and nos genes, and the virA and virB2 genes, allowed fingerprinting of Ti plasmids. Genomic typing by the rapid PCR-RFLP method is thus shown to be useful for an independant identification of strains and of the conjugative Ti plasmids.
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  • 6
    ISSN: 1432-072X
    Keywords: Agrobacterium ; β-1,2-Glucan ; chvB Mutants—Ca2+
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chvB gene of Agrobacterium tumefaciens encodes a 235 kDa proteinaceous intermediate involved in the synthesis of β-1,2-glucan. chvB mutants show a pleiotropic phenotype. Besides not to produce cyclic β-1,2-glucan, chvB mutants have been reported to be avirulent, attachment-deficient, and nonmotile. In this study we report additional differences from the parent strain, probably all linked to changes in the cell envelope. This pleiotropic phenotype — except for attachment and virulence — could largely be prevented by growing chvB cells with low levels of calcium. Although a role for β-1,2-glucan in osmoadaptation has been proposed, the mode of action of β-1,2-glucan is not known. We speculate that in A. tumefaciens β-1,2-glucan stabilizes membranes, which would be important especially in hypotonic media containing calcium.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1573-5028
    Keywords: Agrobacterium ; gene regulation ; sensor protein ; signal transduction ; VirA protein ; acetosyringone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The VirA protein ofAgrobacterium tumefaciens is thought to be a receptor for plant phenolic compounds such as acetosyringone. Although it is not known whether the interaction between VirA and the phenolics is direct or requires other phenolic-binding proteins, it is shown in this study that the first 280 amino acids of the VirA protein are not essential for the acetosyringone mediatedvir gene induction response. Considering the fact that the cytoplasmic region between the amino acids 283 and 304 is highly conserved between the different VirA proteins, and that deletion of this region abolishes VirA activity, we suggest that the acetosyringone receptor domain is located in this cytoplasmic domain of the VirA protein.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant molecular biology 25 (1994), S. 989-994 
    ISSN: 1573-5028
    Keywords: Agrobacterium ; binary vectors ; gentamycin resistance ; kanamycin resistance ; tobacco
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The newpPZP Agrobacterium binary vectors are versatile, relatively small, stable and are fully sequenced. The vectors utilize the pTiT37 T-DNA border regions, the pBR322bom site for mobilization fromEscherichia coli toAgrobacterium, and the ColE1 and pVS1 plasmid origins for replication inE. coli and inAgrobacterium, respectively. Bacterial marker genes in the vectors confer resistance to chloramphenicol (pPZP100 series) or spectinomycin (pPZP200 series), allowing their use inAgrobacterium strains with different drug resistance markers. Plant marker genes in the binary vectors confer resistance to kanamycin or to gentamycin, and are adjacent to the left border (LB) of the transferred region. A lacZ α-peptide, with the pUC18 multiple cloning site (MCS), lies between the plant marker gene and the right border (RB). Since the RB is transferred first, drug resistance is obtained only if the passenger gene is present in the transgenic plants.
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  • 9
    ISSN: 1573-5028
    Keywords: Agrobacterium ; attachment ; plant receptor ; rhicadhesin ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Attachment of Rhizobium and Agrobacterium bacteria to cells of their host plants is a two-step process. The first step, direct attachment of bacteria to the plant cell wall, is mediated by the bacterial protein rhicadhesin. A putative plant receptor molecule for rhicadhesin was purified from cell walls of pea roots using a bioassay based on suppression of rhicadhesin activity. This molecule appeared to be sensitive to treatments with pronase or glycosidase. Its isoelectric point is 6.4, and its apparent molecular mass was estimated to be 32 kDa before and 29 kDa after glycosidase treatment, as determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and ultrafiltration. The sequence of the first 29 N-terminal amino acids was determined: A-D-A-D-A-L-Q-D-L-C(?)-V-A-D-Y-A-S-V-I-L-V-N-G-F-A-S-K(Q)-(P/Q)-(L)-(I). No homology with known proteins was found. In the course of this research project the extracellular matrix protein vitronectin was reported to inhibit attachment of A. tumefaciens to carrot cells [29]. A variety of adhesive proteins, including vitronectin, contain a common cell attachment determinant with the sequence R-G-D. Since we could not detect other cell wall components able to suppress rhicadhesin activity, and since an R-G-D containing hexapeptide was also active as a receptor, we speculate that the plant receptor for rhicadhesin is a glycoprotein containing an R-G-D attachment site.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-203X
    Keywords: Trifoliate orange ; Epicotyl segment ; Agrobacterium ; Transformation ; rolC promoter ; β-glucuronidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple and efficient gene transfer system of trifoliate orange (Poncirus trifoliata Raf.) was developed using epicotyl segments. The segments were infected with Agrobacterium harboring the binary vector pBI121 or pBI101-O12-p1. Both vectors contained the neomycin phosphotransferase II (NPTII) and the β-glucuronidase (GUS) genes. In the plasmid pBI101-O12-p1, the GUS gene was directed to the promoter region of ORF12 (rolC) of the Ri plasmid. On a selection medium containing 100 or 200 μg/ml kanamycin, adventitious shoots were formed from 21.7–44.6% of the segments. Histochemical GUS assay showed that 55.4–87.7% of the shoots expressed the GUS gene. The stable integration of this gene was also confirmed by polymerase chain reaction (PCR) analysis and by Southern blot analysis. When the pBI101-O12-p1 plasmid was used, the GUS activity was found to be located in phloem cells of leaf, stem and root. More than 100 transformed plants were obtained using this method within 2–3 months.
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 88 (1994), S. 433-440 
    ISSN: 1432-2242
    Keywords: Agrobacterium ; Transformation ; Gene expression ; Petunia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic petunia (Petunia hybrida Vilm.) plants were obtained from Agrobacterium-mediated shoot apex transformation. Studies at the phenotypic as well as molecular level established both the presence of the NPT II (neomycin phosphotransferase II) and GUS (β-glucuronidase) genes and their level of activity. Twenty-nine primary transformed plants showed varying patterns of phenotype expression of both genes. NPT II and GUS expression in 7 primary plants over a 4-month interval showed varying levels of gene expression within and among individual plants. All primary transgenic plants were self-pollinated and backcrossed to establish the inheritance patterns of both genes. Mendelian and non-Mendelian inheritance patterns for both genes were observed. Analysis of the progeny showed poor transmission of the foreign genes through the pollen especially when two or more bands were present in the Southern hybridization. Most plants whose progeny segregated in Mendelian ratios for either the NPT II or GUS gene had just one copy of the gene. In this study where both foreign genes were examined in both self and test crosses, no transgenic plant showed Mendelian patterns of inheritance for both foreign traits.
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  • 12
    ISSN: 1432-2242
    Keywords: Agrobacterium ; Iodoacetamide Nicotiana protoplast fusion ; Nitrosomethyl urea Rhodamine 6G ; X-irradiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An effective selection system preceded by double inactivation of parental protoplasts was used to transfer Nicotiana suaveolens Leh. cytoplasmic male sterility into a commercial tobacco (N. tabacum L.) breeding line. Mesophyll protoplasts from transformed plants of N. tabacum cultivar WZ2-3-1-1 possessing a neomycin phosphotransferase II gene were used as the nuclear donors, while those isolated from N. suaveolens plants carrying a chloroplast mutation for resistance to spectinomycin, induced using nitrosomethyl urea, were the cytoplasm donors in somatic cybridizations. Prior to fusion, nuclear donor protoplasts were inactivated with iodoacetamide or rhodamine 6G, while those of the cytoplasm donor were inactivated by X-irradiation. The resultant microcalli were cultured on a shoot regeneration medium containing both kanamycin and spectinomycin to select cybrids. Only regenerants that had typical characteristics of the N. tabacum cultivar were selected for transfer to the glasshouse. Four putative cytoplasmic male-sterile (CMS) plants, out of a total of 44 regenerated plants transferred to the glasshouse, were obtained. Intraspecific somatic transfers of the CMS trait between N. tabacum cultivars with distinctlydifferent morphologies using single inactivation and nonselective shoot regeneration medium were demonstrated. The implications of the results for practical tobacco breeding as a means of circumventing lengthy backcrossing procedures are discussed.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 89 (1994), S. 577-582 
    ISSN: 1432-2242
    Keywords: Wheat ; Transformation ; Agrobacterium ; Electroporation ; Polyethylene glycol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A procedure for culturing protoplasts from slowly growing embryogenic calli of wheat was developed. The procedure was dependent on the ability to isolate large numbers of culturable protoplasts from slowly growing embryogenic callus. Approximately 68% of the isolated protoplasts divided, and 22% formed colonies; of the latter, 67% continued to proliferate. Plating efficiency was reduced when protoplasts were transformed by polythylene glycol, electroporation, and/or Agrobacterium. Intact cells were also directly transformed by electroporation. Direct electroporation of the Agrobacterium binary vector into intact cells resulted in a significant increase of GUS activity over the control.
    Type of Medium: Electronic Resource
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  • 14
    ISSN: 1573-9368
    Keywords: Agrobacterium ; mutagenesis ; Nicotiana plumbaginifolia ; nitrate reductase ; ploidy ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transformation frequencies were determined for 1n, 2n, and 4n Nicotiana plumbaginifolia protoplast cultures inAgrobacterium-mediated gene transfer experiments. An unexpected large drop (50%) in plating efficiencies was observed in the non-selected (control) 1n populations after transformation treatment with virulent strains. This effect was not observed in the 2n or 4n cultures or in the 1n cultures when treated with avirulent bacteria. The mortality was disproportionally high and could not be explained by the low (0.1–0.5%) transformation efficiency in the 1n population, indicating mutagenesis of the cell populations independently from the T-DNA insertions. Mutagenesis was also indicated in gene tagging experiments where nitrate reductase-deficient (NR−) mutants were selected from haploidNicotiana plumbaginifolia protoplasts, as well as from leaf disc cultures or protoplasts of diploid plants that were heterozygotic for a mutation either in the NR apoenzyme gene (nia/wt) or one of the molybdenum-containing cofactor genes (cnxA/wt), afterAgrobacterium co-cultivation. The chlorate-resistant isolates were tested for the T-DNA-specific kanamycin resistance trait only after NR-deficiency had been established. Thirty-nine independent NR-deficient mutants were analysed further by Southern blot hybridization. There was no indication of integrated T-DNA sequences in the mutated NR genes, despite the fact that NR-deficient cells were found more frequently in cell populations which became transformed during the treatment than in the populations which did not. These observations suggest that transformation-competent cells undergo mutagenesis during theAgrobacterium gene transfer process not only as a result of stable integration events, but also through accompanying events that do not result in major changes in the mutated loci. The nature of these changes at the molecular level remains to be elucidated.
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  • 15
    ISSN: 1573-9368
    Keywords: Agrobacterium ; β-glucuronidase ; cytoenzymology ; reporter gene ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For several models expressing theuidA orgus reporter gene with or without a presequence for mitochondrial targeting, we have demonstrated that the compartmentation of β-glucuronidase (E.C. 3.2.1.31) activity was not in agreement within situ localization of the diX-indigo microcrystals generated by the cytoenzymological GUS assay. These crystals were generally associated with the various cytomembranes and lipid inclusions. Experiments with purified β-glucuronidase or withgus-expressing bacteria incubated with 5-bromo-4-chloro-3-indolyl-β-d-glucuronide and maize oil-phosphate buffer emulsion indicated that the intermediate products resulting from the GUS assay actively diffused and crystallized preferentially in association with lipids, sometimes far from the site of enzyme activity. This phenomenon could not be suppressed by the addition of potassium ferricyanide in the incubation medium. These findings are discussed with regard to previously reported biochemical and histochemical data on animal tissues, and focus on the necessity for caution in studies of tissue-specific gene expression using the GUS assay, particularly for lipid-rich plant models.
    Type of Medium: Electronic Resource
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 244 (1994), S. 367-373 
    ISSN: 1617-4623
    Keywords: Agrobacterium ; Nopaline ; Repressor Activator ; LysR family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The NocR protein of Agrobacterium tumefaciens was found to regulate expression of the divergently transcribed nocB and nocR genes of the pTiT37 nopaline catabolism (noc) region. Experiments using the firefly luciferase (luc) gene as reporter demonstrated that NocR represses and activates transcription from the nocB promoter in the absence and presence of nopaline, respectively. NocR also negatively autoregulates its own synthesis irrespective of the presence of nopaline. Regulation of expression of both nocB and nocR is mediated by binding of the NocR protein to the nocR promoter. A 12 by symmetrical sequence, which lies 3 by downstream of the −10 hexamer of the nocR promoter, was confirmed to be essential for binding of the NocR protein. Functional localization of the nocB and nocR promoters verified that they do not overlap at all, and that the interrupted dyad, at which NocR binds, is 137 by upstream of the regulated nocB promoter. The in vivo and in vitro results described here and those published previously suggest that a novel type of regulatory mechanism, which may involve changes in DNA topology, controls gene expression in the noc operon of pTiT37.
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 245 (1994), S. 493-505 
    ISSN: 1617-4623
    Keywords: Agrobacterium ; Bacterial evolution ; Nopaline strains ; Ti plasmids ; Grapevine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Ti plasmid of the Agrobacterium vitis nopaline-type strain AB4 was subcloned and mapped. Several regions of the 157 kb Ti plasmid are similar or identical to parts of the A. vitis octopine/cucumopine (o/c)-type Ti plasmids, and other regions are homologous to the nopaline-type Ti plasmid pTiC58. The T-DNA of pTiAB4 is a chimaeric structure of recent origin: the left part is 99.2% homologous to the left part of the TA-DNA of the o/c-type Ti plasmids, while the right part is 97.1 % homologous to the right part of an unusual nopaline T-DNA recently identified in strain 82.139, a biotype Il strain from wild cherry. The 3′ non-coding regions of the ipt genes from pTiAB4 and pTi82.139 are different from those of other ipt genes and contain a 62 by fragment derived from the coding sequence of an ipt gene of unknown origin. A comparison of different ipt gene sequences indicates that the corresponding 62 by sequence within the coding region of the AB4 ipt gene has been modified during the course of its evolution, apparently by sequence transfer from the 62 by sequence in the 3′ non-coding region. In pTi82.139 the original coding region of the ipt gene has remained largely unmodified. The pTiAB4 6b gene differs from its pTi82.139 counterpart by the lack of a 12 by repeat in the 3′ part of the coding sequence. This leads to the loss of four glutamic acid residues from a series of ten. In spite of these differences, the ipt and 6b genes of pTiAB4 are functional. Our results provide new insight into the evolution of Agrobacterium Ti plasmids and confirm the remarkable plasticity of these genetic elements. Possible implications for the study of bacterial phylogeny are discussed.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 159 (1994), S. 171-178 
    ISSN: 1573-5036
    Keywords: Agrobacterium ; Gigaspora margarita ; Glomus intraradix ; PCR ; mycorrhizae ; rDNA gene ; root organ culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The symbiosis between vesicular-arbuscular mycorrhizal (VAM) fungi and host plants develops after successful interactions between both partners. These interactions probably involve signal molecules produced by the host plant, by the fungi, or by both. So far the biotrophic status of VAM fungi has hampered the understanding of the processes regulating their physiology. However, among different methods for co-cultivating VAM fungi, root organ cultures (ROC) appear to be a useful technique for studying VAM development. This system has been useful in defining the nutritional requirements of VAM fungi in the precolonization stage and in obtaining axenic fungal material in various developmental stages. The work discussed here focuses on the application of Polymerase Chain Reaction (PCR) technology and the potential of promoting hyphal growth in the absence of the plant. These techniques are being used to study VAM fungi in two main areas. The first concerns the determination of the DNA sequences coding for the SSU ribosomal RNA of two VAM fungi. This approach has allowed the design of specific primers for the rapid identification and quantification of VAM fungi. The second area of research concerns the potential use of PCR technology to study selective expression of specific genes during fungal spore development in defined in vitro conditions. The achievement of this future prospect depends on the ability to prepare PCR-based cDNA libraries from small amounts of fungal material after stimulation of hyphal growth with CO2 and plant flavonols.
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  • 19
    ISSN: 1573-5044
    Keywords: Agrobacterium ; Atropa belladonna ; double transformation ; phytohormone content ; tropane alkaloids ; viviparous leaves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hairy root cultures of Atropa belladonna L. were established by infection either with Agrobacterium rhizogenes ATCC 15834 or MAFF 03-01724, and transgenic plants were obtained from both hairy root cultures. Doubly transformed roots were induced by re-infection of the leaf segments of transgenic Atropa belladonna plants (A. rhizogenes 15834) with MAFF 03-01724. Shoots and viviparous leaves were regenerated from the doubly transformed roots. The genetic transformation was determined by the opine assay (agropine, mannopine and/or mikimopine) and polymerase chain reaction. Physiological changes and tropane alkaloid biosynthesis in the hairy roots (singly and doubly transformed) were investigated. The alkaloid content in the doubly transformed root strain was intermediate as compared to the root strains which were singly transformed. On the other hand endogenous IAA levels in doubly transformed roots were significantly decreased compared to both singly transformed roots.
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  • 20
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 10 (1994) 
    ISSN: 1069-8299
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Type of Medium: Electronic Resource
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  • 21
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 10 (1994), S. 1-9 
    ISSN: 1069-8299
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An iterative method is introduced for computing second-order partial derivatives (sensitivities) of eigenvalues and eigenvectors of matrices which depend on a number of real design parameters. Numerical tests confirm the viability of the method and support our theoretical analysis. Alternative methods are reviewed briefly and compared with the one proposed here.
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  • 22
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 10 (1994), S. 11-19 
    ISSN: 1069-8299
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In the formulation of the semi-Loof element the rotation of the tangent plane is derived from the interpolation of the transverse displacement, while the rotation of the normal is interpolated separately by another set of shape functions. The geometric stiffness matrix can be formulated by use of either of the two rotation representations. It is demonstrated that the use of the tangent plane representation in the geometric stiffness matrix is far superior to the common form at present.
    Additional Material: 4 Ill.
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  • 23
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 10 (1994), S. 21-32 
    ISSN: 1069-8299
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An efficient solution to boundary-value problems may be based on the application of a suitably truncated T-complete set of Trefftz functions over individual subdomains and on linking the fields by a least-squares procedure. Although it yields a symmetric system of linear equations, this approach as originally presented by Zielinski and Zienkiewicz is not suited for implementation into FE codes. The present paper presents two equivalent formulations, which take respectively the form of the finite (FE) and non-conventional boundary-element (BE) approach. Both allow the resulting simultaneous equations to be assembled following the standard direct stiffness methods and can readily be implemented into existing FE codes.As in the conventional p-method, the accuracy may be controlled within large limits without increasing the number of elements. The present approach allows substantial saving in computer time in comparison with the so-called hybrid-Trefftz (HT) elements, though the assumed displacement fields are identical. The practical efficiency of the new T-element approach is assessed on the problem of stress concentration in a symmetrically compressed perforated panel.
    Additional Material: 6 Ill.
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  • 24
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 10 (1994), S. 167-178 
    ISSN: 1069-8299
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A sixth-order polynomial shape function is developed for BIE analysis. The function is applied over only three-noded elements, but with the support for the function extending over adjacent elements. This avoids the oscillations near the ends of the range which otherwise are characteristic of high-order polynomial interpolation. Various test problems are explored, and it is shown that results as accurate as those from conventional quadratic elements are obtained with larger nodal spacings, and thus giving the potential for significant reductions in matrix storage requirements and solution times.
    Additional Material: 9 Ill.
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  • 25
    Electronic Resource
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    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 10 (1994), S. 155-166 
    ISSN: 1069-8299
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A finite element formulation based on superposition is proposed for a lifting aerofoil in incompressible potential flow. An accuracy improvement technique for the singularity at the aerofoil trailing edge is discussed. It is shown that the quarter-node quadrilateral element can be readily employed to simulate this singularity. It is also demonstrated that the circulation in the flow field can be easily represented by a single constraint equation rather than introducing an artificial cut line in the mesh. The influence of finite-element mesh size on solution accuracy and the correct form of the boundary condition have been investigated as well. Numerical examples are given for both steady and quasisteady Joukowski aerofoils of various thicknesses and at a range of incidences. In all test cases, good agreement is observed between the analytical solution and the numerical result.
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    Communications in Numerical Methods in Engineering 10 (1994) 
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  • 27
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    Communications in Numerical Methods in Engineering 10 (1994), S. 183-194 
    ISSN: 1069-8299
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An 8-node element (HMITC) for 3D non-linear analysis of solids is presented. The new element is based on an element developed by Wilson and Ibrahimbegovic, that incorporates incompatible modes, and on the method of mixed interpolation of tonsorial components. The HMITC element does not contain spurious zero energy modes and satisfies Irons' Patch Test. The numerical experimentation indicates that the HMITC has good performance even with distorted meshes.
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  • 28
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    Communications in Numerical Methods in Engineering 10 (1994), S. 203-215 
    ISSN: 1069-8299
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In this paper we consider an explicit finite-element method, with elements adaptively oriented in space-time, for the solution of one-dimensional conservation laws, extending previous work dealing with linear convection-diffusion and incompressible flow. In particular we consider Burgers' equation and the compressible Euler equations.
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  • 29
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    Communications in Numerical Methods in Engineering 10 (1994), S. 195-201 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A numerical method, based on neural-network-based functions, for solving partial differential equations is reported in the paper. Using a ‘universal approximator’ based on a neural network and point collocation, the numerical problem of solving the partial differential equation is transformed to an unconstrained minimization problem. The method is extremely easy to implement and is suitable for obtaining an approximate solution in a short period of time. The technique is illustrated with the aid of two numerical examples.
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  • 30
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    Communications in Numerical Methods in Engineering 10 (1994), S. 217-225 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An efficient algorithm for consideration of axial and cyclic symmetry in the boundary-element method is presented. The appropriate transformation relationships was derived. The algorithm is then implemented in a boundary element program for the analysis of 2D elastostatic problems. Through analysis of typical problems the validity of the algorithm and its implementation is verified. A high level of accuracy and substantial reduction in computer time and storage was achieved.
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  • 31
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    Communications in Numerical Methods in Engineering 10 (1994), S. 227-235 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The diffusion of oxygen into an absorbing medium as an example of an implicit moving boundary problem has been dealt with by a number of authors using various numerical techniques, and, where appropriate, approximate analytical expressions. To evaluate the time for complete absorption, extrapolation is usually employed. An unconditionally stable explicit numerical scheme that avoids the limitations of such methods is presented and tested herein. Unlike existing schemes this method is fully numerical; it avoids the large array size, generally required for existing methods, by using a variable-length time step. The time for complete absorption emerges from the final step in the normal computing procedure with no recourse to extrapolation. Furthermore, owing to the implicit condition prevailing at the moving boundary, no iterations are needed to evaluate the time step required for the moving boundary to move a single space increment. The numerical results obtained compare very favourably with those due to earlier authors.
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  • 32
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    Communications in Numerical Methods in Engineering 10 (1994), S. 237-248 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In the paper we have developed a new formulation for solution of structural-acoustic coupling problems by boundary elements using the multiple reciprocity method. It is assumed that the structure is composed of plate components and is excited by the external or the internal noise source. The efficiency of the proposed formulation becomes especially remarkable if the boundary-value problem is to be solved repeatedly for different values of frequency. The accuracy of the numerical computations has been compared with the analytical solution in a test example.
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  • 33
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    Communications in Numerical Methods in Engineering 10 (1994), S. 257-265 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Techniques for stress- and strain-controlled in situ homogenization of inelastic periodic composites are presented. The results of homogenization computations on a specific elastoplastic composite solid are then employed to validate the form of an orthotropic elastoplasticity model with a tensorial kinematic hardening law.
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  • 34
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    Communications in Numerical Methods in Engineering 10 (1994), S. 249-255 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An algorithm for evaluation of friction in general contact-impact interfaces is described. The algorithm is based on an explicit finite-element method. Coulomb's friction law is assumed. The defence node algorithm is used such that the sticking condition can be imposed with the Lagrange multiplier method even in explicit dynamic analysis. The algorithm is supposed to be applicable in general situations, including large deformations of the contact-impact bodies and large relative sliding between the contact-impact boundaries. Numerical results are presented to demonstrate the performance of the algorithm.
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  • 35
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    Communications in Numerical Methods in Engineering 10 (1994), S. 275-277 
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  • 36
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    Communications in Numerical Methods in Engineering 10 (1994) 
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  • 37
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    Communications in Numerical Methods in Engineering 10 (1994), S. 267-273 
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    Topics: Mathematics , Technology
    Notes: A generalization of the r(1-m)/m strain singularity of higher-order isoparametric elements is presented. It is shown that, by variable placement of the side nodes between their original and singular positions, the point of singularity sensed by the element can be controlled. The transition elements have a strain singularity outside their domain. The singular and non-singular elements are elements are special cases of the general mapping. The transition elements, together with the singular isoparametric elements, can be used for solving crack problems.
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  • 38
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    Communications in Numerical Methods in Engineering 10 (1994), S. 279-290 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: In the design of members with flaws, it is necessary to keep the stress intensity factor K of any sharp crack below the fracture toughness Kcr of the materials. Stress-intensity factor equations for the more common basic specimen geometries and various loading conditions are available in the literature. The application of these equations to complex structures involves geometric problems such as the identification of the outline of each member and the sizing of the equivalent specimen for each flaw. The paper gives a response to such difficulties.
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    Communications in Numerical Methods in Engineering 10 (1994), S. 303-312 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A simple, yet storage-effective ‘linear’ programming code is given. The assumption of non-negative variables is bypassed without increasing the size of the problem. Furthermore, the objective is allowed to be summed over not just linear, but also concave, functions. A specific truss topology optimization example is shown.
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    Communications in Numerical Methods in Engineering 10 (1994), S. 291-296 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: We propose in this paper a nine-point, fourth-order difference method for the numerical solution of the quasilinear Poisson equation \documentclass{article}\pagestyle{empty}\begin{document}$$ Au_{zz} + \frac{1}{r}u_r + Bu_{rr} = f\left({r,z,u,u_r,u_z} \right) $$\end{document} with appropriate boundary conditions. The method is based on five evaluations of f. The numerical results of four problems obtained using this method are listed. The results demonstrate the fourth-order accuracy of the method.
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  • 41
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    Communications in Numerical Methods in Engineering 10 (1994), S. 297-302 
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    Notes: Singularly perturbed second-order elliptic equations with boundary layers are considered. These may be considered as model problems for the advection of some quantity such as heat or a pollutant in a flow field or as linear approximations to the Navier-Stokes equations for fluid flow. Numerical methods composed of central-difference operators on special piece-wise-uniform meshes are constructed for the above problems. Numerical results are obtained which show that these methods give approximate solutions with error estimates that are independent of the singular perturbation parameter. An open theoretical problem is posed.
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  • 42
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    Communications in Numerical Methods in Engineering 10 (1994), S. 313-320 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: In the paper we present a superconvergent patch recovery technique for obtaining higher-order-accurate finite-element solutions and thus a postprocessed type of L2 norm error estimate. Two modifications make our procedure different from the one proposed by Zienkiewicz and Zhu (1992), in which higher-order-accurate derivatives of the finite-element solution at nodes are determined. Firstly, the recovery process is made for element, not for nodes. An ‘element patch’, which represents the union of an element under consideration and the surrounding elements, is introduced. Secondly, the local error estimate is calculated directly from the improved solution for this element. Numerical tests on both 1D and 2D model problems show that this method can provide an asymptotically exact a posteriori L2 norm error estimate if the used element possesses superconvergent points for the solutions.
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    Communications in Numerical Methods in Engineering 10 (1994), S. 321-331 
    ISSN: 1069-8299
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A new approach to developing serendipity quadrilateral infinite elements is presented. Using these elements universal matrices for quasiharmonic equation are developed. For a particular member of the family these matrices are independent of the size and shape of the element. Using these matrices the element stiffness matrix can be generated in a simpler manner by taking into account the size and shape of the element.
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    Communications in Numerical Methods in Engineering 10 (1994), S. 333-338 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A new global secant relaxation (GSR)-method-based improvement procedure is used to improve the overall convergency performance of the modified Newton-Raphson iteration in carrying out the solution of discrete systems resulting from the finite-element discretization of a certain class of structural problems involving non-linear deformation behaviour.
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    Communications in Numerical Methods in Engineering 10 (1994), S. 339-353 
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    Topics: Mathematics , Technology
    Notes: Theoretical and experimental analysis of free-surface electrohydrodynamic flow is fragmented and incomplete. Simulation studies of this phenomenon are further limited by the inherent complexities in the modelling process. In this note a mathematical model is developed to analyse free-surface electrohydrodynamic flow in two dimensions, and preliminary results of the simulation are described. The configurations examined include electrified conducting surfaces, the dielectrophoretic forces, and a conducting jet. The simulation is compared with analytical results in the first two investigations and is shown to be quite accurate. In the last simulation it is demonstrated that in the initial formation of a conducting jet, a 10 per cent increase in applied voltage results in about a 10 per cent increase in fluid velocity.
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    Communications in Numerical Methods in Engineering 10 (1994), S. 355-357 
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    Communications in Numerical Methods in Engineering 10 (1994), S. 359-360 
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    Communications in Numerical Methods in Engineering 10 (1994) 
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    Communications in Numerical Methods in Engineering 10 (1994), S. 361-371 
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    Topics: Mathematics , Technology
    Notes: The dual reciprocity boundary element method, first proposed by Nardini and Brebbia (1982, 1985), is a powerful technique for solving elliptic partial differential equations. Adopting this approach, a singular volume integral, which needs to be evaluated with a traditional boundary element method, can be converted into a boundary integral. However, when the governing equation is of a certain type, this conversion fails due to the singularities being introduced inside the physical domain and on the boundary arising by differentiating distance functions. We avoid these artificially created singularities by constructing a transformation which leads to improved numerical results.
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    Communications in Numerical Methods in Engineering 10 (1994), S. 743-749 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: This is a study about one of the core questions in the GMRES(k) method regarding the obtaining of vector yk for the least-squares problem, argminy |Hky - β(n)e1|2 (see Saad and Schultz1). We propose a simple but efficient approach to the resolution of this problem and a low cost computation of the residual and the residual norm, including both in a complete and detailed FGMRES(k) algorithm. The whole algorithm of minimization only involves two backward substitutions with triangular matrices and a dot product. The residual and the residual norm are computed, making use of results in the least-squares problem.
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    Communications in Numerical Methods in Engineering 10 (1994), S. 759-760 
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    Communications in Numerical Methods in Engineering 10 (1994), S. 761-762 
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    Communications in Numerical Methods in Engineering 10 (1994) 
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  • 54
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 1481-1497 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A procedure for the optimization of stretched triangular grids is described. The method is based on the construction and minimization of a function that represents a generalized version for stretched grids of a non-linear spring system. The function is minimized using a gradient method based on the steepest descent. Examples are provided to show the applicability of the method to computational fluid dynamics problems.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 1465-1480 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Multigrid method is used to accelerate the convergence of the numerical solution of the set of partial differential equations for generating the Body-Fitted Co-ordinate system (BFC) for both simply and doubly connected domain problems. The multigrid method is based on the full approximation scheme with bilinear interpolation as prolongation operator. The other components of the multigrid method such as the relaxation method, restriction operator, number of relaxation sweeps per cycle and direction of relaxation sweeps are varied with a view to obtaining the general principle in implementing an efficient multigrid algorithm for the generation of the BFCs.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 1531-1555 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: In Part I of this work, Paulino et al.1 have presented an algorithm for profile and wavefront reduction of large sparse matrices of symmetric configuration. This algorithm is based on spectral properties of a Finite Element Graph (FEG), An FEG has been defined as a nodal graph G, a dual graph G* or a communication graph G· associated with a generic finite element mesh. The novel algorithm has been called Spectral FEG Resequencing (SFR). This algorithm has specific features that distinguish it from previous algorithms. These features include (1) use of global information in the graph, (2) no need of a pseudoperipheral vertex or the endpoints of a pseudodiameter, and (3) no need of any type of level structure of the FEG. To validate this algorithm in a numerical sense, extensive computational testing on a variety of problems is presented here. This includes algorithmic performance evaluation using a library of benchmark test problems which contains both connected and non-connected graphs, study of the algebraic connectivity (λ2) of an FEG, eigensolver convergence verification, running time performance evaluation and assessment of the algorithm on a set of practical finite element examples. It is shown that the SFR algorithm is effective in reordering nodes and/or elements of generic finite element meshes. Moreover, it computes orderings which compare favourably with the ones obtained by some previous algorithms that have been published in the technical literature.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 1557-1572 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: To exploit the benefits of parallel computer architectures for multibody system simulation, an interdisciplinary approach has been pursued, combining knowledge of the three disciplines of dynamics, numerical mathematics and computer science. An analysis of the options available for the formulation and numerical solution of the dynamical system equations yielded a surprising result. A method initially proposed to solve the inverse problem of dynamics is the best choice to generate the system equations required for solving the simulation problem, when relying on implicit integration routines. Such routines have the particular advantage of handling stiff systems, too. The new O(N)-residual formalism, generating the system equations in a form required for implicit numerical integration, has a high potential to benefit from parallel computer architectures. Two strategies of medium and coarse grain parallelization have been implemented on a Transputer network to obtain a package for parallel multibody simulation. An analysis of the performance of this package demonstrates for typical multibody simulation problems that the new code is five times faster than existing codes when implemented on a serial computer. An additional speed-up by the same order of magnitude is obtained when the code is implemented on a Transputer network.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 181-201 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A general Finite Volume Method (FVM) for the analysis of structural problems is presented. It is shown that the FVM can be considered to be a particular case of finite elements with a non-Galerkin weighting. For structural analysis this can readily be interpreted as equivalent to the unit displacement method which involves mainly surface integrals. Both displacement and mixed FV formulations are presented for static and dynamic problems.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 229-256 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: An element-free Galerkin method which is applicable to arbitrary shapes but requires only nodal data is applied to elasticity and heat conduction problems. In this method, moving least-squares interpolants are used to construct the trial and test functions for the variational principle (weak form); the dependent variable and its gradient are continuous in the entire domain. In contrast to an earlier formulation by Nayroles and coworkers, certain key differences are introduced in the implementation to increase its accuracy. The numerical examples in this paper show that with these modifications, the method does not exhibit any volumetric locking, the rate of convergence can exceed that of finite elements significantly and a high resolution of localized steep gradients can be achieved. The moving least-squares interpolants and the choices of the weight function are also discussed in this paper.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 37-48 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A pseudo-transient (static) linear, geometric, material and combined geometric and material non-linear analyses of composite laminates are presented. A nine-noded isoparametric quadrilateral finite element belonging to the Lagrangiar family is used in space discretization. An explicit time marching scheme is employed for time integration of the resulting discrete ordinary differential equations with the special forms of diagonal fictitious mass and/or damping matrices. Elasto-plastic material behaviour is incorporated using the flow theory of plasticity. In particular, a modified version of Hill's initial yield criterion is used in which anisotropy parameters of plasticity are introduced. The shear deformation is accounted for by assuming a constant transverse shear strain across the thickness of the laminate and the geometric non-linearity is considered in the sense of von Karman strains. The layered element approach is adopted for the treatment of plastic behaviour through the thickness. A wide range of numerical examples is presented to demonstrate the validity and efficiency of the present approach. The results for combined non-linearity are also presented. The variety of results presented here, which are based on realistic material properties of often-used advanced laminated composite plates, and especially those for combined non-linear analysis, should serve as a reference for future investigations.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 91-105 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A variationally coupled finite element-boundary element method is developed for transient problems. A single variational statement is obtained for the entire domain and the unknown tractions, which may be discontinuous on the interface and are often a source of difficulties, are eliminated. Moreover, no interface conditions need be taken into consideration at the level of the discretized equation. The discrete equations for the coupled system can be obtained directly without any intermediate steps. The method generalizes a coupling method previously developed by the authors for statics. Numerical examples show that the solutions obtained by the present method agree very well with those obtained by analytical solutions.
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  • 62
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 431-455 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A six-node triangle plate/shell element is developed for the analysis of laminated composite structures. This model is formulated using Hamilton's principle along with a first-order (Reissner/Mindlin) shear deformation theory. The element is based upon an isoparametric representation along with an interdependent interpolation strategy; bicubic polynomials for the transverse displacement and biquadratic polynomials for the element geometry, in-plane displacements and rotations. The resulting element, which is evaluated using exact numerical integration, has correct rank and is free of shear ‘locking’. Numerical results are presented that validate the new element and prove its outstanding convergence capabilities in comparison to existing triangular elements using standardized test problems (elastic eigenvalue analysis, patch test, static simply supported square-plate solutions) and experimentally measured vibration data of cantilevered isotropic and composite plates.
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  • 63
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 475-496 
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    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The collapse of ordinary finite elements to generate the desired strain singularity at the crack tip for fracture mechanics applications can lead to unwanted additional singularities in that area. Although neither the quarter-point nor the half-point 8-node two-dimensional (2-D) and 20-node three-dimensional (3-D) elements exhibit this behaviour, the present article proves that arbitrarily small deviations from the quarter-point element can be constructed which do have additional singularities. Since the general behaviour of the half-point element is not affected by small modifications, this element is better suited to match complex body geometries.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 537-538 
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  • 65
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 517-536 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The superconvergent patch derivative recovery method of Zienkiewicz and Zhu is enhanced by adding the squares of the residuals of the equilibrium equation and natural boundary conditions. In addition, a new conjoint polynomial for interpolating the local patch stresses over the element which significantly improves the local projection scheme is presented. Results show that in the 4-node quadrilateral, the equilibrium and boundary condition residuals usually improve accuracy but not the rate of convergence, whereas in the 9-node quadrilateral, results are mixed. The conjoint polynomial always improves the accuracy of the derivative field within the element as compared to the standard nodal interpolation, particularly in 4-node quadrilaterals.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 279-296 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: An hybrid stress element formulation based on internal, incompatible displacements is used to develop efficient Mindlin plate elements. The 4-node quadrilateral Mindlin plate element is derived from a modified energy functional. Both displacements and stresses are defined in the natural co-ordinate interpolation system. The assumed stress field is obtained by tensor transformation and so chosen as to ensure that the element is co-ordinate invariant and stable. Shear locking is avoided through an appropriate identification of the internal, incompatible displacement field. The role played by incompatible displacements in the formulation of hybrid stress elements for thin and moderately thick plates is discussed. Numerical applications are presented to illustrate the accuracy and reliability of the suggested Mindlin plate element.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 605-621 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: In the present paper, we analyse the computational performance of the Lanczos method and a recent optimization technique for the calculation of the p (p ≤ 40) leftmost eigenpairs of generalized symmetric eigenproblems arising from the finite element integration of elliptic PDEs. The accelerated conjugate gradient method is used to minimize successive Rayleigh quotients defined in deflated subspaces of decreasing size. The pointwise Lanczos scheme is employed in combination with both the Cholesky factorization of the stiffness matrix and the preconditioned conjugate gradient method for evaluating the recursive Lanczos vectors. The three algorithms are applied to five sample problems of varying size up to almost 5000. The numerical results show that the Lanczos approach with Cholesky triangularization is generally faster (up to a factor of 5) for small to moderately large matrices, while the optimization method is superior for large problems in terms of both storage requirement and CPU time. In the large case, the Lanczos-Cholesky scheme may be very expensive to run even on modern quite powerful computers.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 387-411 
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    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A new eight-node quadrilateral shear-bending Reissner-Mindlin plate finite element for the very thin and thick plates without locking and spurious zero-energy modes is presented. The element has very good convergence characteristics both for thin and thick plates, is hardly insensitive to mesh distortions, and passes the patch tests. The formulation of the element is derived from a displacement variational principle and some general criteria to compute inconsistent transverse shear strains. These criteria have been applied with success to four- and eight-node quadrilateral plate finite elements and could be applied to construct triangular elements. The eight-node quadrilateral shear-bending plate finite element proposed has been found to be very efficient.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 457-474 
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    Notes: A theory is proposed in this paper to explain, in a unified manner, the three rational approaches using the incompatible displacements uλ in the hybrid element formulation. Limited to the plane stress case, the theory suggests that the introduction of uλ is to constrain the total value of the invariant I2(= σxσy - τxy2) of the assumed stresses over the element to zero. In the plane stress case, this is the well-known condition for the stress distributions to be independent of the elastic constants of the material. The theory proposed is also used to explain the closeness of the performances of the four 4-node hybrid stress membrane elements, 5β-I, 5β-II, 5β-A and 5β-C, that have been developed previously. The differences among them are shown to be of the second degree in the distortion measures of the element shape, when they are compared on the basis of the non-vanishing total value of I2 of their respectively adopted assumed stress field. Several numerical examples are used to verify and to illustrate the proposed theory.
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    International Journal for Numerical Methods in Engineering 37 (1994) 
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 1825-1840 
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    Notes: This paper presents a boundary element formulation for the permanent Navier-Stokes equations in which the well-known closed-form fundamental solution for the steady Stokes equations is employed. In this way, from the integral representation formulae for the Stokes' equations, an integral equation is found in which the original non-linear convective terms of the Navier-Stokes equations appear as a domain integral. Additionally, the method of dual reciprocity is used to transform the domain integral to boundary integrals (this method is closely related to the method of particular integrals also used in the literature to transform domain integrals to boundary integrals). Numerical results are presented for the three-dimensional internal flow in a cylindrical container with a rotating cover, in which the accuracy of the method is shown.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2291-2309 
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    Notes: The helicoidal beam model developed in the first part of this work is applied here to the development of a mixed finite element for space-curved and twisted beams undergoing large displacements and finite rotations. Starting from the governing weak form expressed by the principle of virtual work, a consistent linearization is obtained in the following and a novel updated Lagrangian finite element implementation is thoroughly discussed.The unique features and the distinguishing properties previously claimed for the helicoidal model are shown here to imply remarkable numerical consequences. For this purpose, meaningful example problems regarding the non-linear static response of beams are addressed in the following and the results are compared with those available from the literature.Furthermore, a finite element in time for the rigid body dynamic problem is developed within the framework of the helicoidal geometry. The underlying philosophy of this novel finite element for dynamics is the realization of the helicoidal decomposition of the rigid body motion within a time step.
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    International Journal for Numerical Methods in Engineering 37 (1994) 
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2311-2335 
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    Notes: A finite element approach for shape optimization in two-dimensional (2-D) frictionless contact problems is presented in this work. The goal is to find the shape that gives a constant distribution of stresses along the contact boundary.The whole formulation, including mathematical model for the unilateral problem, sensitivity analysis and geometry definition is treated in a continuous form, independently of the discretization in finite elements. Shape optimization is performed by direct modification of geometry through B-spline curves and an automatic mesh generator is used at each new configuration to provide the finite element input data for numerical analysis and sensitivity computations. Using augmented-Lagrangian techniques (to solve the contact problem) and an interior-point mathematical-programming algorithm (for shape optimization), we obtain several results reported at the end of the article.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2337-2338 
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 1881-1896 
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    Notes: This paper presents the first endeavour to exploit a generalized differential quadrature method as an accurate, efficient and simple numerical technique for structural analysis. Firstly, drawbacks existing in the method of differential quadrature (DQ) are evaluated and discussed. Then, an improved and simpler generalized differential quadrature method (GDQ) is introduced to overcome the existing drawback and to simplify the procedure for determining the weighting coefficients. Subsequently, the generalized differential quadrature is systematically employed to solve problems in structural analysis. Numerical examples have shown the superb accuracy, efficiency, convenience and the great potential of this method.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 3015-3018 
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 3019-3021 
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    International Journal for Numerical Methods in Engineering 37 (1994) 
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 3005-3014 
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    Notes: Since Soize introduced the concept of fuzzy structures in structural acoustics there has been little activity clarifying the basic elements which underlie his theory. Soize's papers are not easy reading due to the high level of mathematical formalism. In addition Soize simultaneously bases this fuzzy structure theory on two components: (1) a model for one Degree Of Freedom (DOF) fuzzy oscillators, and (2) a medium frequency solution method developed previously. It is unclear as to the role of the two components, although others have already undertaken a study of the medium frequency method by itself.In the present paper a fundamental analysis of the first component, the one-DOF fuzzy oscillators, is undertaken. The symbolic manipulation program Mathematica is utilized to gain insight into this component of Soize's fuzzy theory. The resulting Mathematica simulations are easy to use and interpret, and they provide valuable insight into the parameters composing Soize's fuzzy oscillators. It is determined that in many cases of structural acoustics, where there is small damping and a medium to high modal density, the fuzzy mass primarily determines what effect a discrete fuzzy oscillator will have as an attachment.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 3023-3036 
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    Notes: A new numerical approach has been devised for performing accurate absorbing-emitting non-scattering radiation calculations for Cartesian co-ordinate based problems with one ordinate symmetry using only the solution plane formed by the other two ordinates. To accomplish this, the method makes use of ‘effective intensity rays’ which only exist and have meaning in the solution plane of the problem but which are made, through a special procedure, to contain the information of the third dimension. This novel procedure thus eliminates the problem of solid angles and greatly reduces both the complexity and computational expense involved in the solution of such problems. Yet it can do this without producing loss in accuracy. The approach is ultimately intended for use in combustion problems involving high temperatures and optically thin media. For verification purposes the method is applied to the problem of heat exchange in infinitely long rectangular enclosures and is found to give excellent results over a optical thickness range that includes the optically very thin case.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 1978-1979 
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2525-2525 
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2005-2039 
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    Notes: A method is described which constructs three-dimensional unstructured tetrahedral meshes using the Delaunay triangulation criterion. Several automatic point creation techniques will be highlighted and an algorithm will be presented which can ensure that, given an initial surface triangulation which bounds a domain, a valid boundary conforming assembly of tetrahedra will be produced. Statistics of measures of grid quality are presented for several grids. The efficiency of the proposed procedure reduces the computer time for the generation of realistic unstructured tetrahedral grids to the order of minutes on workstations of modest computational capabilities.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2109-2123 
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    Notes: A coupled transient thermoelastic behaviour of an axial-cracked hollow circular cylinder subjected to a sudden heating is investigated in this study. It is shown that surface heating may induce the compressive thermal stress near the inner surface of the cylinder which in turn may force the cracked surfaces to close together. Assuming that the existence of the crack does not alter the temperature distribution, we can divide this problem into two parts and solve it by the principle of superposition. First, the temperature and transient thermal stress distributions along the axisymmetric surface of the imaginary cylinder without crack are obtained by finite element implicit time integration method Secondly, the opposite sense of the stress distributions along the cracked surfaces, which is obtained previously, is treated as the traction boundary conditions; the contact length and contact pressure of the real cracked cylinder are obtained by modified elimination finite element scheme. Finally, we also obtained the normalized stress intensity factor for the crack tip of the cylinder. It is concluded that the effect due to thermoelastic coupling term on stress intensity factor becomes more important for higher coupling coefficient, and this coupling term also results in a small time lag in temperature, thermal stress and stress intensity factor.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2149-2150 
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2699-2700 
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2583-2608 
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    Notes: In continuum mechanics within specific classes of problems, one- or two-dimensional theories are often simpler to apply than the more complete three-dimensional one. This is, for example, the case of thin bodies, such as plates or shells, which may be studied using appropriate two-dimensionai theories.Within this approach, the reduction of the dimension is traded for a loss of information relative to the motion in the transverse direction. For example, in the case of non-linear material behaviour, classical plasticity plate theories are usually not able to model the effects related to the spreading of plasticity through the cross-section.In the present paper we discuss a generalized plasticity plate model, which can be used to reproduce some of the three-dimensional effects in a two-dimensional setting. We present the continuous and the discrete time model, including both isotropic and kinematic hardening mechanisms; moreover, the form of the tangent matrix consistent with the discrete model is addressed.Finally, some examples (cantilever beam, clamped circular plate and clamped square plate under monotonic and cyclic loading) are studied numerically using a three-dimensional classical plasticity theory, a classical plasticity plate theory and the proposed plate theory. The generalized plasticity plate model matches the three-dimensional response with greater accuracy, than the classical plasticity plate model.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2679-2697 
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    Notes: The dynamic behaviour of liquid-filled shells of revolution is investigated considering the soil-structure interaction and the fluid-structure interaction, respectively. In the circumferential direction the loads and variables are approximated by Fourier series. The shell is modelled through shell ring elements including non-linear behaviour, coupled with isoparametric continuum ring elements and special infinite elements for the soil and isoparametric pressure ring elements for the fluid. Transient loadings like earthquake excitation and the non-linearities of the shell and the soil require an analysis in the time domain. To reduce the size of the problem, linear parts of the system are condensed by the substructure technique. The soil region is divided into two parts, a near field permitting non-linearities like plastification or uplifting of the shell, and a far field for the treatment of radiation of energy.The boundary conditions for the shell footing have a strong influence on the distribution of the axial membrane forces and, hence, on the stability limit, which is mostly governed by plastic collapse and caused by the dynamically activated pressure acting on the tank wall. It is shown how the soil properties influence the dynamic stability of the shell under harmonic excitation and under realistic earthquake motion.
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2701-2702 
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    International Journal for Numerical Methods in Engineering 37 (1994), S. 2717-2733 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper presents a Wave Equation Model (WEM) to solve advection dominant Advection-Diffusion (A-D) equation. It is known that the operator-splitting approach is one of the effective methods to solve A-D equation. In the advection step the numerical solution of the advection equation is often troubled by numerical dispersion or numerical diffusion. Instead of directly solving the first-order advection equation, the present wave equation model solves a second-order equivalent wave equation whose solution is identical to that of the first-order advection equation. Numerical examples of 1-D and 2-D with constant flow velocities and varying flow velocities are presented. The truncation error and stability condition of 1-D wave equation model is given. The Fourier analysis of WEM is carried out. The numerical solutions are in good agreement with the exact solutions. The wave equation model introduces very little numerical oscillation. The numerical diffusion introduced by WEM is cancelled by inverse numerical diffusion introduced by WEM as well. It is found that the numerical solutions of WEM are not sensitive to Courant number under stability constraint. The computational cost is economical for practical applications.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
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  • 94
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 37 (1994), S. 3825-3842 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A flat shell element based on the free-formulation finite element concept is developed for analysing geometrically non-linear thin composite shells. A corotational form of the updated Lagrangian formulation is utilized. Numerical results for typical validation problems are presented in order to demonstrate the accuracy and validity of this element. These results are obtained by solving the incremental equilibrium equations through the cylindrical arc-length method.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
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  • 95
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 37 (1994), S. 3809-3823 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In this paper we investigate the additional storage overhead needed for a parallel implementation of finite element applications. In particular, we compare the storage requirements for the factorization of the sparse matrices that would occur on a parallel processor vs. a uniprocessor. This variation in storage results from the factorization fill-in. We address the question of whether the storage overhead is so large for parallel implementations that it imposes severe limitations on the problem size in contrast to the problems executed sequentially on a uniprocessor. The storage requirements for the parallel implementation are based upon a new ordering scheme, the combination mesh-based scheme. This scheme uses a domain decomposition method which attempts to balance the processors' loads and decreases the interprocessor communication. The storage requirements for the sequential implementation is based upon the minimum degree algorithm. The difference between the two storage requirements corresponds to the storage overhead attributed to the parallel scheme.Experiments were conducted on regular and irregular, 2-D and 3-D problems. The meshes were decomposed into 2-256 subdomains which can be executed on 2-256 processors, respectively. The total storage requirements or fill-in for most of the 2-D problems were less than a factor of two increase over the sequential execution. In contrast, large 3-D problems had zero increase in storage or fill-in over the sequential execution; the fill-in was less for the parallel execution than the sequential execution. Thus, we conclude that the storage overhead attributed to the use of parallel processors will not impose severe constraints on the problem size. Further, for large 3-D applications, the combination mesh-based algorithm does better than minimum degree for reducing the fill-in.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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  • 96
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 37 (1994), S. 3843-3868 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Incrementally objective integration schemes are proposed for the accurate and efficient determination of design sensitivity coefficients (DSCs) for solid mechanics problems with both material and geometrical non-linearities. The derivation of these schemes are based on the direct differentiation of objective schemes that are used in stress analysis for problems of this class. Two widely used objective stress rates, the Jaumann rate and the Green-Naghdi rate, are considered here within the same setting with only minor changes of the integration scheme. Numerical results are presented for a simple shear problem with different material constitutive laws, including a hypoelastic model and a unified isotropic viscoplastic model, for these two objective rates. The numerical results are compared with analytical solutions or direct integration solutions. The close agreement among these solutions demonstrates the accuracy and efficiency of the proposed scheme.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
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  • 97
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 37 (1994), S. 3869-3903 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Development of a finite deformation elasto-plasticity model based on the multiplicative decomposition of the deformation gradient is presented and discussed in detail. The formulation presented in this paper includes the derivation of the full set of equations for the Drucker-Prager yield criterion. The equations, which are not available elsewhere, are developed within a framework using a spectral decomposition approach. Further, expressions for the consistent (algorithmic) tangent moduli in the finite strain regime are developed. Since the finite deformation framework employed to obtain the expressions presented here collapses to the classical infinitesimal plasticity framework when the finite strain assumption is no longer necessary, the finite deformation consistent tangent moduli are compared to the consistent tangent moduli valid for use with infinitesimal plasticity. Validation of the implemented finite deformation elasto-plastic Drucker-Prager model is performed through the solution of the concrete slump test. Comparisons between an existing approximate analytical solution and experimental data are presented, and results are discussed in detail.
    Additional Material: 11 Ill.
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  • 98
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 37 (1994), S. 3905-3919 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: This paper presents a parallel implementation of the boundary element method for MIMD computer architectures to determine the effective properties of two heterogeneous physical systems. The first physical system is comprised of spheres sedimenting in a viscous fluid at low Reynolds numbers. The effective property is characterized by the hindered settling function which is a measure of the average sedimentation velocity. The second physical system is a short-fibre reinforced composite. The effective property for this system is the composite modulus. The determination of effective properties of heterogeneous media requires performing statistical analyses of several realizations of physical systems based on defining characteristics of the media. The boundary element method is particularly well suited for studying such systems because of the simplification in the discretization associated with the method. However, as the number of heterogeneities to be modeled is increased so are the computational demands. Parallel computation offers the opportunity to model systems of greater complexity. We discuss a parallel boundary element formulation based on the torus-wrap mapping. In this approach, blocks of the coefficient matrix associated with the discretized boundary element equations are assigned to processors as opposed to more traditional parallel boundary element implementations where rows or columns are assigned to processors. The torus-wrap mapping can be shown to minimize communication volume between processors during the LU factorization. Therefore, the present formulation scales well with increases in the number of processors.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 99
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 37 (1994), S. 4263-4284 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The use of inconsistent displacement fields for Mindlin plate elements causes unmatched coefficients to appear in shear strain interpolations. The role of the numerical integration order in relation to these unmatched coefficients, and the existence of optimal stress points are explained through the use of the reduced minimization concept. This concept makes it possible to test whether assumed displacement fields or shear strain fields result in inconsistent strain fields which contain spurious constraints. We have included applications of reduced minimization to the conventional C0-continuous elements, which employ reduced integration, and to quadrilateral Mindlin plate elements of Hinton and Huang to demonstrate how these elements alleviate shear locking.
    Additional Material: 4 Ill.
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  • 100
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 37 (1994), S. 715-716 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Type of Medium: Electronic Resource
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