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  • 1960-1964  (78)
  • 1890-1899
  • 1963  (39)
  • 1961  (39)
  • Biochemistry and Biotechnology  (78)
Materialart
Erscheinungszeitraum
  • 1960-1964  (78)
  • 1890-1899
Jahr
  • 1
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 309-330 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Biological purification of water pollutants depends upon a complex balance of organisms and environment conducive to effective metabolism. The bench scale test is useful to determine effects of a given waste water on that balance and to suggest controls promoting optimum purification. Applicability of laboratory results depends, in part, upon an experimental design similating anticipated operating conditions. This paper discusses advantages and limitations of historical bench-tests as a guide for design of tests giving data that are more useful during full-scale operation.
    Zusätzliches Material: 5 Tab.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A 50-gal. growth chamber is described together with its air filter and humidifier. The unit was designed especially for the production of Neurospora crassa, and was found to be effective in producing bulk amounts of mycelia with a high specific activity of tryptophan synthetase.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 87-89 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Addition of the S-2-hydroxyethyl-, S-2-chloroethyl-, S-n-propyl-, and S-iso-propyl-derivatives of homocysteine, and methoxinine to S. aureofaciens (ATCC 13900) fermentations resulted in production of 7-chloro-6-demethyltetracycline in addition to the normally synthesized 7-chlortetracycline.
    Zusätzliches Material: 1 Tab.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 123-129 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A method of inoculating nutrient surfaces uniformly with bacterial cells has been developed. The method is based on controlled exposure of samples in a humidified chamber to a spray produced from a bacterial suspension. Factors affecting uniformity of inoculation (ionic strength of suspension, force of spray, distance between spray nozzle and sample, and exposure time) were studied in detail. Under optimum condition using agar medium in Petri plates, differences between total counts for six plates inoculated simultaneously, or between counts for individual 2 cm.2 areas taken at any location on any of the plates, were not statistically significant at the 5% level. The coefficient of variability for counts on 2 cm.2 areas renged from 8 to 12% between tests of 216 counts each.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 8
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 185-192 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: By growing Aspergillus parasiticus (C.M.I. 15957) under controlled conditions on sterilized peanuts an average of 265 mg. of aflatoxin per kilogram of peanuts has been produced and subsequently isolated. Other strains from the A. flavus-oryzae group gave lower yields of aflatoxin and one such strain gave aflatoxin from which certain normal components were absent. The aflatoxin produced on sterilized peanuts by any particular strain of A. flavus-oryzae was shown by thin-layer chromatography to contain the same major components as were produced by that strain on unsterilized whole peanuts.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 9
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 221-230 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A process has been developed giving over-all yields of about 50%: which could be increased to about 80% with higher material costs and processing time. The preferred method of recovering nucleotides from the cytoplasm has been thermal extraction by means of a heat exchanger followed by filtration, protein precipitation, and carbon adsorption. The concentrated eluate from the carbon is absorbed onto Dowex 1 ion-exchange resin, and groups of nucleotides are removed by pH-defined eluents and recovered by a second carbon adsorption. Finally, standard ion-exchange resolutions have been used to recover individual nucleotides. Other biochemicals in the extract have been investigated, and preliminary work has been done on the combination of nucleotide extraction with the recovery of nucleic acids from the residual cells.
    Zusätzliches Material: 3 Tab.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 231-242 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: In the present paper the authors described laboratory fermentors used in the Antibiotics Research Institute since 1958 for research concerning the technology of the biosynthesis of antibiotics and amino acids and other aerobic fermentations. The lids of the fermentors are made from stainless steel, the vessels are made from glass. The fermentors are placed, always four each, in eight water baths with automatic temperature regulation within a range of ±0.5°C. Details regarding construction, auxiliary equipment, and working procedures used for the sterilization of fermentors and media, inoculation, injection of additives, and defoaming are given. The set-up for one-stage and multi-stage, batch and continuous cultivation are discussed.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 11
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 12
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 131-146 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A sensitive cup plate assay method for lysine on large plates using S. faecalis ATCC 6057 as the test organism is described in detail. Well-defined zones of dense growth were obtained in a rectilinear dose - response range from 5 to 3000 μg. of L-lysine/ml. Comparative assays on twelve different samples were carried out using the turbidimetric tube assay method with Leuc. mesenteroides ATCC 8042 and the cup plate method with S. faecalis ATCC 6057. Good agreement between the two assay methods was obtained for five samples. For the remaining seven samples the tube assay values were appreciably higher than those obtained by the plate method. This discrepancy is discussed. Pure lysine added to various sample extracts could be quantitatively recovered. The specificity of the lysine requirement of S. faecalis ATCC 6057 in the plate assay was also investigated. Under certain experimental conditions, a weak and variable response was obtained with D-lysine and the polyamines cadaverine, spermine, and spermidine. There was generally no difficulty in distinguishing the D-lysine and polyamine growth zones from the L-lysine growth zones. This growth effect of D-lysine and polyamines could be diminished and even completely abolished by increasing the DL-hydroxylysine hydrochloride level in the plate medium. The lysine peptides glycyl-L-lysine and L-lysylglycine gave excellent growth zones of the same appearance as those produced by L-lysine. The activity of these two peptides in cup tests were on a molar basis about 70% of that of L-lysine.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
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  • 13
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 9-15 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A method by which a paramagnetic oxygen analyzer is used to determine oxygen consumption and carbon dioxide production during aerobic fermentation of Torulopsis utilis is described. The volume of carbon dioxide produced is not necessarily related to the volume of oxygen consumed by the microorganisms. For this reason, all carbon dioxide is removed from the effluent gas before it enters the analyzer when determining oxygen consumption. A method for calibrating the analyzer to correct for this removal is outlined. When determining carbon dioxide, effluent gas bypasses the carbon dioxide absorption tower before entering the analyzer. The carbon dioxide which it contains dilutes the oxygen remaining in the gas. The magnitude of this dilution can be used to calculate the percentage of carbon dioxide produced in terms of the air flow to the fermentor. Equipment required to alternately measure and record oxygen consumption and carbon dioxide production is described.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
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  • 14
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 21-25 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Cobamide production by Butyribacterium rettgeri Barker and Haas (ATCC 10825) was studied on a laboratory scale. Yields of the order of 5 μg./ml. were obtained when this anaerobe was grown for 6 days at 37°C. in a medium containing glucose, cornsteep liquor, cobalt nitrate, calcium carbonate, and sufficient ammonium hydroxide to keep the pH between 6.0 and 7.2. All of the vitamin was found associated with the bacterial cells, and examination of extracts of these cells showed the presence of 5,6-dimethylbenzimidazolylcobamide coenzyme in large quantities. Supplementation of the fermentation medium with a number of benzimidazoles resulted in formation of traces of “new” cobamides with these bases in the nucleotide protion of the cobamide. Supplementation with a number of purines did not result in incorporation of these moieties in the cobamide portion of the molecule.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
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  • 15
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 49-52 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: An investigation was made of the stability of product limited continuous cultures of Saccharomyces carlsbergensis and Pseudomonas ovalis. No oscillations were found in pH or glucose concentration during the steady state, and no overshoot resulted from changing from one steady state to another.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 16
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 41-48 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Bacillus subtilis NRRL B-2612, isolated at this laboratory, was used to produce polyglutamic acid from devitalized wheat gluten. Conditions for production of the polypeptide were investigated. When NRRL B-2612 was grown on wheat gluten in shaken flasks with phsophate buffer and salts at pH 6.5, yields of polymer reflected a 25-40% incorporation of glutamic acid into polyglutamic acid. Repeated alcoholic precipitation yielded peptides containing between 45 and 90% D-glutamic acid. Physical and chemical properties of a typical purified polyglutamic acid indicated that the product was a gamma-linked polypeptide composed of 75% D-glutamic acid and 25% L-glutamic acid with a molecular weight of approximately 200,000.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
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  • 17
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 109-121 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Microbiological production of beta-carotene previously investigated in shakenflask culture has been scaled-up to 20-liter fermentors containing an operating volume of 10-liters. Yields obtained in shaken flasks, approximately 100 mg./100 ml. have been reproduced with a fermentation time of 72 hr. Factors affecting the fermentation: inoculum, mash ingredients, and environmental conditions, were investigated. Typical data are presented. A preliminary cost analysis indicates a “cost to make” of $31.35/kg. of carotene contained in the dried fermentation solids.
    Zusätzliches Material: 12 Ill.
    Materialart: Digitale Medien
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  • 18
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 167-184 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Shock pressure waves with a frequency of 50/sec. and a maximal pressure of 60-70 kg./cm.2 greatly reduced the viability of E. coli. A similar effect was obtained also on staphylococci, enterococci, Salmonella Zanzibar, and coliphages T2. On the contrary no effect on spores of B. subtilis was found. The effect was practically independent of the maximal pressure within the range from 30-80 kg./cm.2, the highest pressure tested. The effect decreased with increasing cell density of the bacterial suspension. Bacteria surviving shock treatment died considerably more rapidly when stored at 4°C. than bacteria treated in the same way except for exposure to pressure waves. Morphological examinations with the aid of ordinary light microscopy as well as electron microscopy suggest that the effect was due to mechanical action on the bacterial cell walls.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
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  • 19
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963) 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 20
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 287-307 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Sphaerotilus is usually present in biological waste treatment processes and is considered to be a nuisance organism because it may cause bulking of activated sludge. However, it may play an important role in the decomposition of putrescible organic matter in properly operated biological waste treatment processes. The research described herein is a study of the variety of types of organic compounds which may be decomposed by Sphaerotilus and the effect of some environmental factors upon the rate of decomposition. Although there are some types of compounds which cannot be decomposed by Sphaerotilus, it is shown that this organism is able to assimilate and oxidize a large variety of organic compounds and that it is tolerant of an extremely wide range of environmental conditions. The role of Sphaerotilus in biological waste treatment is discussed in the light of the data presented.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 21
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 355-365 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: It has been found that a bacterium (isolant C12B) grew poorly on 6 and 8 carbon alcohols, well on 10 and 12 carbon alcohols, and slowly but well on the 14 to 18 carbon compounds. Unexpectedly, resting cells oxidized the 6 and 8 carbon compounds most readily and cell-free extracts oxidized 6, 8, and 10 carbon alcohols. Extracts from cells adapted to sodium lauryl sulfate oxidized the 12 carbon alcohol as well, if small concentrations of substrate were used. The bacterium grew on benzene sulfonate and adapted resting cells oxidized that compound. Pure culture degradation studies revealed that isolant C12B used only 11% of tetrapropylene ABS supplied as a sole carbon source, whereas 66% of sodium lauryl sulfate was degraded. Intermediate quantities of kerosene-based alkyl benzene sulfonate and a tallow-derived detergent were utilized. Extracts of another bacterium, TEG-5, grown on tetraethylene glycol, contained enzymes which oxidized polyethylene glycols up to an average molecular weight of 600, but not any larger polymers. This oxidation was visualized by coupling to reduction of ferricyanide.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
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  • 22
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 17-20 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The applicability of physicochemical adsorption laws to the sorption of methylene blue by dead Penicillium chrysogenum was studied. The specific area (area/mass) of the mold was measured.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
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  • 23
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 147-165 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A number of factors was found to affect the cup plate assay for lysine with Streptococcus faecalis ATCC 6057. Hydroxylysine, aspartic acid, and Tween 80 were found to be among the essential components of the assay medium. Removal of any of these three components produced diffuse growth zones or otherwise unsatisfactory plates. The inclusion of asparagine and additional dipotassium phosphate to the medium was found to be advantageous but not an absolute requirement. It was important not to autoclave the hydroxylysine and the additional dipotassium phosphate together with the rest of the assay medium, but to add these components to the sterilized medium immediately before pouring the plates. With certain concentrations of hydroxylysine in the medium, the addition of L-glutamine was found to potentiate the effect of hydroxylysine and thereby improve the lysine plates. The effect of graded amounts of the above mentioned medium components and of the pH of the assay medium was also studied. Increasing the sterilization time of the medium from 5 to 10 min. at 120°C. improved the readability of the lysine plates and led to more easily reproducible results. Factors influencing the preparation of the inoculum for the lysine plates were also studied: the age of the stab culture, the amount of inoculum used per plate, and the influence of the nature of the suspension medium used for washing the inoculum.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
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  • 24
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 193-199 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Ferrocyanide-treated beet molasses was fermented in fully baffled mechanically agitated, 2.5-liter, tank-type fermenters using pellets of Aspergillus niger grown in shake flasks as an inoculum. The object of the study was to adapt the fermentation method previously developed in an unstirred tower-type fermenter to a conventional tank-type fermenter. Under selected conditions of stirring (agitator speed 400-700 rpm) and sparging (medium porosity sintered disk), high yields of citric acid (8.6% citric acid, 70% conversion of available sugar) were obtained in 140 hr. of fermentation. There was little relation between citric acid yield and aeration conditions in the fermenter as judged by mash viscosity measurements and sulfite oxidation studies.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
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  • 25
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 53-58 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The design of a cabinet for the aseptic manipulation of material is described. The interior surfaces of the cabinet are sterilized by swabbing with a germicide. Germ-free conditions are maintained during operation by means of a positive pressure of sterile air. A fan and filter unit attached to the cabinet is used to blow sterile air into the cabinet in sufficient quantity so as to flow out of the hand ports at a velocity of 50 ft./min. In the design, careful consideration was given to the ease of manipulations in the cabinet.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 26
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Removal of the pungent factor, allyl isothiocyanate, will partly determine whether mustard seed can become a commerical source for oil and meal in the United States. In processing studies at the Northern Laboratory, the mustard glucoside was converted enzymatically and the pungent oil was removed. This process has now been extended to pilot-plant scale by using filtration-extraction equipment at the Southern Laboratory. After desolventization and further steam stripping, the extracted meal had a residual content of 2.9% crude fat and 0.004% allyl isothiocyanate.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 27
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 59-74 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The distribution of dissolved oxygen in agitated, sparger-aerated aqueous solutions has been investigated by polarographic techniques. The results of the investigations are discussed in relation to earlier studies in bubble distribution in such systems. Liquid-phase mixing is good at agitation intensities well below that necessary to disperse bubbles throughout the liquid.Suspended solids interfere with the transfer of oxygen unless they are freely suspended. Discrete fibers (paper-pulp) are much easier to suspend than molds in either filamentous or pellet forms. Mass-transfer at the cell-liquid interface appears to be unaffected, in both bacterial and mold suspensions, by the intensity of agitation, provided that the organisms are freely suspended.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
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  • 28
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 83-85 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The inclusion of ethionine in Streptomyces rimosus fermentation resulted in the production of N-methylethyloxytetracycline. The compound has been isolated, crystallized, and a number of its chemical properties determined.
    Zusätzliches Material: 1 Tab.
    Materialart: Digitale Medien
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  • 29
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 101-107 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: An adsorption colorimetric method is proposed for the determination of the percentage of dead cells in suspensions of Penicillium chrysogenum, based on the observed fact that a linear relation exists between that percentage and the dye concentration at the equilibrium point.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 30
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 91-99 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Microbial polysaccharides which have many potential industrial applications are receiving more and more attention. One of the economic obstacles to the commercialization of these polysaccharides has been the cost of isolating them from fermented broth. To reduce this cost a recycling process was developed. The polyanionic polysaccharide synthesized from glucose by the activity of the bacterium Xanthomonas campestris NRRL B-1459 is precipitated from the fermented broth with a quaternary ammonium compound (QAC).QAC is removed from the precipitate with methanol leaving the water-soluble polysaccharide. Evaporation of the methanol wash removed the methanol and substantially all the QAC was recovered in the concentrate. This was recycled for subsequent precipitations. Chemical assay and the viscosity of water solutions of the isolated polysaccharide indicate no adverse effects on it from recycled QAC. The cost to make this polysaccharide by the described procedure is estimated to be $1.14/lb. This cost estimate includes land, buildings, raw materials, equipment, labor and supervision, utilities, factory supplies, working capital, and plant overhead.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 31
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 211-220 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A disposable plastic tetrahedron bag is described which is suitable for holding a 50 ml. culture on a shaker table. Bags can be made out of polypropylene or Teflon and they are quite simple and inexpensive. The tetrahedrons make excellent use of the shaker table space and they give a threefold increase in aeration efficiency over Erlenmeyer flasks.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 32
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 243-251 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The metabolic pathway of degradation of naphthalene to salicylic acid has been studied by several investigators. The rates of conversion and of cell growth are the basis for the present study. In the early phase of the fermentation, the salicylic acid accumulation followed an exponential curve, as did the bacterial cell accumulation. In the second phase, most of the salicylic acid was produced according to a linear or zero reaction, at rates of 0.3 to 0.5 g./l./hr. A linear accumulation of microbial cell weight accompanied the linear salicylic acid formation. Despite the correlation of growth and salicylic acid production, the type or amount of nitrogen supplied for growth had only a small effect on yield over broad limits. The optimum pH for salicylic acid accumulation was approximately 6.5. Below this value a slower rate of production and lower final yield was seen. Above pH 6.5, a rapid degradation of salicylic acid was observed. A temperature range from 25 to 30°C. was optimum. At 35°C., no growth occurred, while at 20°C. very slow salicylic acid accumulation resulted.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 33
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 255-273 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Stabilization ponds have been widely accepted in recent years, following studies that established deeper understanding of the phenomena associated with the stabilization process. A compilation of information on the use of both sewage and industrial ponds is presented showing types and geographical location in the United States. A total of 1,304 stabilization ponds currently are used as the principal method of sewage treatment serving a population of 2,138,085. Ponds are used for tertiary treatment serving a population of 2,146,951, in 343 instances. Use of 827 industrial ponds by 31 industries in 44 States is also indicated. Indiana ranks first in usage with 159 ponds and Callfornia second with 140. Ponds were classed as effective by 66 percent of the 455 users who reported on this item. Of 149 reports on odors, 73% indicated nuisances. Insect problems were reported in only a small number of instances. Data are presented on design considerations and the mechanism of waste stabilization ponds. Aerobic ponds are given greatest emphasis. Photosynthesis and its dependence upon the algal mass, suitable temperature, incident light penetration, nutrient supply, and induced vertical mixing by wind are of prime importance in the stabilization mechanism. Odors are associated with prolonged anaerobic conditions, and these may persist up to 4 weeks following extended ice cover in cold climates, if BOD loadings are 25 lbs. per acre per day or greater. Nitrogen and carbon may be limiting factors in the development of an algal mass. Coliform, BOD, and nutrient removals are presented for various physical, chemical, and climatic conditions. Although fecal streptococci removal averaged 97% in a study pond, they were still discharged in the effluent at a rate of 700,000 per second in winter and 70,000 per second in summer. A striking similarity exists generally among the algal speciation in stabilization ponds, regardless of geographic location. The algal mass is, however, dependent upon unique pond conditions and location, and may vary upwards to nearly 5 million algal cells per milliliter, 34,000 p.p.m. by volume, or 30-35 tons per acre per year. The problem of blue-green algal mats and resultant pigpen odors in extremely localized areas is duscussed. Midges and midge larvae may have an impact on the nutrient cycle in a stabilization pond, and may pose a potential localized nuisance in some areas. Mosquito problems have usually been associated with growth of aquatic vegetation. Suggestions for minimizing the potential problem are discussed.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
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  • 34
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 201-209 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: It is a well-known fact that choosing a sequence of optimal control variables in each reactor tank of a multistage reactor system can result in a considerable increase of the desired product for a consecutive reaction system. The discrete maximum principle is applied to a generalized optimization problem of first order consecutive biochemical reaction system. A detailed numerical solution as an example is also presented for illustration.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
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  • 35
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 275-285 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Phenol-bearing waste waters continue to be an area of wide concern for various industries faced with the task of cleaning up effluents containing relatively small amounts of these materials. The microbiological aspects of the degradation of these materials in pure culture and waste treatment plants is reviewed. Actual operating experiences at three plants of The Dow Chemical Company, where phenols are present in combination with other widely varying waste constituents, are discussed.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
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  • 36
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 331-345 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: These experiments show that ABS can be adsorbed and desorbed from microbial cell surfaces. The adsorption equilibria between the free and adsorbed phases are primarily influenced by the pH of the system. The effects of ABS upon the biochemical activities of bacteria depended upon the amount of ABS adsorbed and the type of bacteria present. The behaviour of ABS is essentially the same whether in the presence of pure or mixed bacterial cultures. Two desorption experiments with soil from a reclaimed water spreading basin which has received sewage over a six month period showed that at least 82% of the ABS “loss” was recoverable from the soil. In all the experiments carried out in this study no evidence was obtained for the biochemical degradation of ABS.
    Zusätzliches Material: 15 Ill.
    Materialart: Digitale Medien
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  • 37
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 347-354 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: With the emphasis being given to the development of biologically soft detergents, it is necessary to establish eriteria defining biodegradability of these materials. It is the authors' opinion that a biologically soft detergent should have the following characteristics: (1) Under normal operations the material should be 85% removable by activated sludge. (2) Normally high discharges of the detergent should not influence biological activity. (3) The detergent should ultimately degrade to carbon dioxide and water.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 38
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 367-384 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Because branched-chain alkyl benzene sulfonate (ABS) is well known to create water pollution problems in surface water and ground water, a study was made on the biodegradation of this surfactant along with other anionic and nonionic surfactants in detergents by water bacteria. Biodegradation was evaluated by the conventional river die-away test employing the methylene-blue method or surface-tension techniques as analytical tools. Under anaerobic conditions, sugar detergents (sucrose esters) seem to be an ultimate solution to water problems created by branched-chain ABS; in aerated water, straight-chain ABS, sulfo tallow methyl esters, and nonionic surfactants are preferable to branched-chain ABS to improve water problems.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 39
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 5 (1963), S. 1-7 
    ISSN: 0006-3592
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A cost analysis of the continuous fermentation of penicillin G shows that materials cost and product yields are more significant than throughput rate. Number of fermentation stages, productivity, and the implications for research and development are discussed.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 40
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 51-63 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Industrially important polysaccharides are now primarily obtained from plant sources. One exception is the exocellular bacterial polysaccharide, dextran. A survey at the Northern Laboratory revealed that many other microbial polysaccharides could be synthesized by the action of micro-organisms on starch-derived saccharides. Previous publications from this laboratory describe the production of the microbial polymer, phosphomannan, synthesized by the yeast Hansenula holstii. This paper describes the production of another polysaccharide, synthesized by the bacterium Xanthomonas campestris NRRL B-1459.Fermentation of media containing 3 per cent dextrose, seeded with 5 per cent inoculum of X. campestris NRRL B-1459 and cultured aerobically at 28°C, is complete in 96 h. A light tan polymer is recovered in yields of 50 per cent, based on commercial dextrose, from the viscous fermentation broth by precipitation with methanol in the presence of an electrolyte. The viscosities of aqueous re-solutions containing 1 and 2 per cent of the polymer were 3,000 cP and 11,000 cP respectively.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 41
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 79-85 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Various studies have revealed the presence of several maltolproducing compounds in streptomycin preparations. These previous studies have utilized the techniques of counter-current distribution, column chromatography, paper chromatography, and electrophoresis. A system of cation-exchange chromatography has been reported which separates, at least partially, six components of the streptomycin complex. This technique involves the use of a gradient elution procedure.In addition to five maltol-producing compounds, another member of the complex is reported. The possible significance of such a compound is discussed.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
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  • 42
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 115-117 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Zusätzliches Material: 1 Tab.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 43
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961) 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 44
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 311-323 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The effectiveness of beta-propiolactone (BPL) and ethylene oxides as sterilants for liquid microbiological media was studied. Preliminary tests against heavy suspensions of B. coagulans in various media confirmed the sterilizing levels found by earlier investigators. In actinomycin fermentation tests, both compounds were found to sterilize effectively at concentrations up to 0·5 per cent without significant effects on the growth-promoting qualities of the medium. At the concentrations required to sterilize large numbers of spores (0·5-1·0 per cent), however, both agents exhibit inhibitory effects on the fermentation. In these cases BPL is less damaging than ethylene oxide.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
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  • 45
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 351-355 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Based on numerous reports in the literature, it appeared that the commerical production of dihydroxyacetone by microbial fermentation was not feasible due to the comparatively long duration of fermentation and the low total yield that could be obtained per fermentor. A new study has indicated that by adjusting environmental conditions and by modifying mash ingredients, commerical production of dihyroxyacetone via microbial oxidation of glycerol is practicable. The effect of these variables on the rate and extent of dihydroxyacetone production is discussed. The commerical and pharmaceutical uses of dihydroxyacetone and its derivatives is presented.
    Zusätzliches Material: 3 Tab.
    Materialart: Digitale Medien
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  • 46
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 219-233 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Several strains of Pseudomonas, Nocardia and incompletely identified soil isolates have been grown in a mineral salts plus hydrocarbon medium, and the fatty acids produced by the organisms have been isolated, identified and estimated. The results of an estimation of the percentage conversion to these acids under varying experimental conditions is discussed in relation to the metabolic systems involved. Some indication has been obtained that the hydrocarbon breakdown pathway by these organisms is that of ω oxidation followed by β oxidation. Preliminary experiments carried out with one strain of organism, Pseudomonas aeruginosa 5940, indicate that there may be some difference in the utilization of odd- and even-chain hydrocarbons by this organism. An improvement of 3 ·8-fold was obtained by using nitrate instead of ammonium nitrogen; 13-fold by using continuous instead of batch operation; and 8-fold by the use of liquid instead of solid paraffins, giving a total improvement of yield of 400-fold.
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
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  • 47
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 235-240 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: This paper presents a method for measuring the specific area (area/mass) of micro-organisms and the average area of cells, based on the adsorption of dyes.
    Zusätzliches Material: 1 Tab.
    Materialart: Digitale Medien
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  • 48
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The redox potential changes in the oxidative fermentation of L-sorbose to 2-keto-L-gulonic acid were investigated, using a mutant strain of Pseudomonas. The growth medium contained sorbose as the sole carbohydrate source. It was found that the redox potential curve of the fermenting system indicated qualitatively the existing oxygen demand of the culture. Increasing aeration rates led to higher oxygen demand and lower redox potentials. A comparison of the redox potential curves revealed that although the amount of dissolved oxygen directly affected the respiration of the culture, it did not necessarily mean respiratory insufficiency. The redox potential difference between the aerated and the deoxygenated (nitrogen flushed) fermentation broth was found to be linearly proportional to the logarithm of the dissolved-oxygen concentration. This observation served as the basis of a new quantitative method of dissolved-oxygen measurement, for which the name differential redoxymetry was proposed.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
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  • 49
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 325-329 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 50
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 95-109 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A production model consisting of one or more exponential decay functions is proposed to represent the rate of accumulation of a product per unit weight of cell. This, together with a cell age distribution function, is used to describe the product accumulation in a fermentation process.This postulate offers a unified concept applicable to both batch and continuous processes as well as problems arising from environmental changes. The idea is compatible with the current understanding of microbial physiology. It offers an interpretation for the controversy over cell-product ratios observed in some batch and continuous fermentations when treated as conventional chemical reaction. The proposed postulate fits reasonably well with the lysine and lactic acid processes.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
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  • 51
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961) 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 52
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961) 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 53
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 7-20 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A cation exchanger suitable for decontaminating low and medium radioactive waste water was sought. Regeneration being considered undesirable, the exchange material had to be cheap and readily obtainable. Sugar-beet pulp, a weakly acidic cation exchanger, satisfies these conditions. Its capacity is about 0·62 mg eq. per gram of dry matter. In order to study the selectivity of the sugar-beet pulp exchanger, the equilibrium curve of the reaction was determined.This curve was found to have the shape of the hyperbola proposed by Waterman and Weber for the characterization of the course of simultaneous reactions. Sugar-beet pulp adsorbs the salts of the alkaline earth metals selectively in the presence of both Na+ and La+++. Sugar-beet pulp was used to decontaminate a solution containing 140BaCl2 and 140LaCl3 and having an activity of about 10-2 μc/ml. The results were satisfactory.By treating sugar-beet pulp with formaldehyde and hydrochloric acid, the amount of water bound to the pulp is very much decreased. By this treatment a cation exchanger is produced having a capacity per unit volume about six times greater than that of sugar-beet pulp. The capacity of this exchanger is about 0·5 mg eq. per gram of dry matter. The selective behaviour of sugar-beet pulp treated with formaldehyde is similar to that of unmodified sugar-beet pulp.By treating sugar-beet pulp with formaldehyde, hydrochloric acid and dilute sulphuric acid, a cation exchanger with very good mechanical and filtration properties is obtained. This exchanger has a capacity of about 1·3 mg eq. per gram of dry matter.It seems possible to employ the cation exchangers obtained by treatment of sugar-beet pulp with either formaldehyde and HCl or formaldehyde, HCl and H2SO4 also for purposes other than the removal of radioactive cations from water.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
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  • 54
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 1-6 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: An empirical relationship of oxygen transfer rate and mould viscosity is discussed, which leads to equations for predicting the OTR of mould fermentations.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 55
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 405-433 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Two commercially available ultrasonic instruments are described, in which micro-organisms and other cells may be disintegrated.Sound waves (20 kc/s) are generated in the cell suspension by means of titanium velocity transformers (probes) coupled to a magnetostriction transducer. One instrument operating at 500 W will disintegrate from 2 to 500 ml, the other operating at 50 W from 1 ml to 30 ml.Yeast was used as a test organism but results on some other organisms are also given. Cell rupture is shown to be independent of sonically generated free radicals, but enzyme inactivation (alcohol dehydrogenase) is accelerated by free radicals. Increasing the viscosity, decreasing surface tension of the suspending medium, or the presence of CO2 decreases disintegration. The addition of solid nuclei such as powdered glass, as well as small air bubbles, increases disintegration.This is consistent with disintegration being due to cavitation, but does not indicate the precise mechanism of cell rupture. Some effects of sonic disruption are compared with disruption by other methods.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
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  • 56
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 125-137 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A modified pH electrode assembly is desccribed which is capable of steam sterilization and which can be installed in steel fermentors of all sizes in a variety of ways. Its design is such that electrode life is prolonged and contamination hazards are reduced.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 57
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The combination of low-temperature disintegration with gradient and pile centrifugation makes possible the preparation of bacterial cell walls under conditions preventing digestion.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
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  • 58
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 181-197 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Accumulation of volatile acids produced during anaerobic fermentation of distillery-spent liquor decreased the activity of Desulphovibrio desulphuricans employed in previously communicated studies; this caused a decrease in the reduction of sulphates to sulphides. An enriched strain of Desulphovibrio rubentschikii made in a synthetic mineral medium (NH4Cl, 1·0 g; MgSO4·7H2O, 2·0 g; NaCl, 10·0 g; CaSO4, 1·0 g; calcium acetate, 1·0 g; K2HPO4, 0·5 g; Mohrs' salt, trace in 1 l.) using calcium acetate as the substrate and carbon from the tap-water was gradually built up to a 3-1. volume. The amount of hydrogen sulphide in this medium was found to be 225 mg/l. The fermentation was next carried out in a synthetic medium containing volatile acids from distillery-spent liquor as the only substrate. A 3-1. fermentation set up with diluted distillery-spent liquor (300 ml or raw liquor in 3,000 ml of water), neutralized with NaOH to pH 7·2 and supplemented with calcium sulphate and 0·1 per cent urea, showed sulphide productions (calculated as milligrams of hydrogen sulphide per litre of raw distillery-spent liquid) of 2,000, 4,000 and 6,000 with 0·1, 1·0 and 2·0 per cent calcium sulphate in the charge respectively. The volatile acid content, expressed as acetic acid, was 18,564 mg/litre of raw distillery-spent liquor on an average throughout the study. The ratio of hydrogen sulphide produced per litre of raw distillery-spent liquor to the amount of volatile acids developed expressed in milliequivalents per litre of raw liquor during fermentation increased gradually from 0·1367 to 0·5567 in the presence of 0·1 to 2·0 per cent of calcium sulphate in the charge.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 59
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 261-275 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Two methods have been investigated for the measurement of dissolved oxygen in fermentations: the tubing method and the probe method. The sensing elements for both methods are steam-sterilizable and measure only the oxygen tension in the liquid phase. The tubing method is probably the most accurate and reliable method available for measurement of dissolved oxygen in fermentations but it requires extensive ancillary apparatus.
    Zusätzliches Material: 12 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 60
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 119-123 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: An analytical method has been devised for the rapid determination of prednisolone produced in a fermentative oxidation process. It allows frequent checking of concentrations of the prednisolone formed during the oxidation of hydrocortisone.The estimation of prednisolone in the presence of hydrocortisone is based upon the diversity of colour shown by the chromogens produced from these steroids by sulphuric acid and ethanol. Using the optimal reagent mixture and conditions, the interference of hydrocortisone is negligible. The steroids are extracted from the fermentation broth with ethyl acetate. One test takes about 20 min.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 61
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 161-174 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The preparation of soybeans for fermentation is a very important part of the process for making miso, a traditional Japanese food. In general, soybeans from the United States are smaller and more pigmented than Japanese soybeans. Twenty-eight American varieties, four Japanese varieties and one Chinese variety have been studied with respect to absorption of water and cooking. A variety of soaking and cooking conditions have been investigated. Some U.S. varieties absorb water and cook unevenly. Unevenness in cooking is not associated with the size of the beans within a variety.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
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  • 62
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 199-218 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A process is described for the production of a specific-molecular-weight dextran, e.g. clinical dextran, by using a modified form of controlled synthesis. The synthesis is carried out directly in a medium containing appropriate amounts of sucrose and low-molecular-weight dextran after inoculation with a culture of actively growing bacteria. The importance of an inoculum substantially freed from substances affecting the synthesis of dextran is demonstrated. The technique appears to offer a decided advantage over other well-known procedures. Technical details of the process and equipment are given.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
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  • 63
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 139-150 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The constant-rate operation of a system utilizing asbestos filter-pads for the cold sterilization of a tissue culture medium is described. Filtration theory is briefly reviewed with regard to the constant-rate process, and the results of several data treatment methods are reported. The standard law of Hermans and Bredée4, 5 best describes the constant-rate data. Computation of the data, when plotted by the standard-law method, results in two straight lines divided by a transition zone. The second region is characterized by a slower plugging rate and a plot of the standard-law slopes versus the initial filtration pressures shows a linear relation. The constant-rate method is compared with the constant-pressure method and is found to yield equivalent overall filtrate rates at lower operating pressures.The observed filtration behaviour is discussed, and available data are presented to indicate the sterilization capability of the filter.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
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  • 64
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 175-180 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A simple procedure for the disintegration of yeast cells, by which litre quantities of cell extract (approximately 11 per cent dry weight) may be obtained, is described. The fresh yeast cake is treated with dry ice in a high speed electric homogenizer after which it is left to thaw out at 0°. No addition of buffer has to be made, and the cell juice obtained after centrifugation of the resulting slurry appears to contain different subcellular particles and various enzymes in a relatively native state.When the extract is incubated at higher temperatures, proteolysis will rapidly change the electrophoretic and enzymatic properties, which indicates that procedures involving autolysis may be dangerous when information about the chemical composition of the native enzymes is desired.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
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  • 65
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 339-350 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A novel fermentor is described in which aerobic microorganisms are grown on solid medium. Cellulose sponges support a nutrient-agar medium, resulting in a large surface area for growth within a relatively small volume. The sponges are alternately squeezed and relaxed within a piston-and-cylinder-type apparatus during fermentation. Provision is made for inoculation, harvesting, and for supplying air during operation. The apparatus has been operated semicontinuously in that there are enough cells left in the sponges after harvest to inoculate fresh medium. Cell concentrations of Serratia marcescens up to 84 × 1010 per ml have been recovered. A growth curve for this micro-organism is given and sample data on semicontinuous operation are presented.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 66
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 357-376 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: When trying to turn a batch fermentation process into a continuous process, many important factors must be repected. A new approach is presented by means of which it is possible to reproduce in a continuous system those changes (both inside the cells and in the environment) which are functions of elapsed time (lag-type changes, sudden changes occurring at a constant growth rate of the micro-organisms, etc.). Special attention is paid to lag-type changes. In the hitherto described apparatuses for continuous cultivation it was difficult to follow changes which are functions of time. The use of a multi-stage apparatus is therefore suggested, and a method of cultivation, which through adjusting retention times in individual cultivation vessels allows a detailed study of such changes, is proposed. Basic equations concerning the growth of microorganisms in the described apparatus are derived and some examples of the possible practical use of the new approach are presented.
    Materialart: Digitale Medien
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  • 67
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 395-403 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: To collect microbes from suspensions by a process other than centrifugation, it has been found effective to add inorganic and organic electrolytes in the colloidal state. The addition of electrolytes causes a neutralization of charges on the particles in suspension resulting in their coagulation and precipitation. High-molecular-weight compounds which form micelles also promote coagulation and precipitation. It is believed that chemical collection is an extremely efficient method of treatment for the mass production of microbial suspensions.
    Materialart: Digitale Medien
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  • 68
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 331-338 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A simple apparatus for preparing shake cultures of microorganisms on a laboratory scale is described. Using a series of special shaking vessels, each of a capacity of 1-10 l., a total volume of 20-200 l. of culture can be produced in one run. The rate of aeration is similar to the one observed in shake flasks.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
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  • 69
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 377-393 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The purposes of this investigation were to determine the relationship between weight and volume increases which take place when wheat kernels are immersed in water, to develop an equation for the volume increase, and to test experimentally its validity.It was shown that the volume increase was practically equal to the weight increase divided by the fluid density. In view of this, the following equation was obtained by analogy to the diffusion equation derived by Becker.1 \documentclass{article}\pagestyle{empty}\begin{document}$$ 1 - \bar V = \frac{2}{{\sqrt \pi }}\frac{S}{V}\sqrt {D_\upsilon \theta } $$\end{document} where 1 - V̄ is the relative volume increase, and Dv is the coefficient of volume increase.The average coefficients of volume increase are given by the Arrhenius relation as: \documentclass{article}\pagestyle{empty}\begin{document}$$ \begin{array}{l@{\quad}l} D_\upsilon = 1{\cdot}068\ e^{ - 10,129/RT} & \hbox{for Ponca wheat} \\ D_\upsilon = 1{\cdot}240\ e^{ - 11,030/RT} & \hbox{for Seneca wheat} \end{array} $$\end{document}The results obtained in this investigation provide useful information for designing the steeping vessels.
    Zusätzliches Material: 10 Ill.
    Materialart: Digitale Medien
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  • 70
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 21-28 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A rapid analytical method has been developed to follow the biological oxidation of 17-α-oxy-11-desoxy-corticosterone (Reichstein compound S) to hydrocortisone. Paper chromatography is too slow to follow the progress of a preparative microbiological steroid transformation. However, 17-α-oxy-11-desoxy-corticosterone can be separated in a sufficiently pure state for photometric assay by a simple extraction from fermentation broths containing several steroids. This extraction, coupled with the formation of a sulphuric acid chromogen, permits estimation of the steroid in fermentation liquors. The steady decrease of the 17-α-oxy-11-desoxy-compound thus estimated proved to be a true indication of the progress of the oxidation process, allowing it to be stopped at the most favourable time.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
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  • 71
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 29-39 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Laboratory scale experiments have been carried out with sulphate-reducing bacteria growing under anaerobic conditions to investigate the removal of iron from iron-containing solutions. The complete removal of iron, as the insoluble sulphide, has been achieved from both ferrous and ferrous-ferric sulphate solutions. It has been found that under certain conditions a magnetic iron sulphide is produced. This aggregates under the influence of a magnetic field and settles at a rate of some 30 in./min. The magnetic sulphide has only been obtained from intermittent continuous cultures. With the system employed, rates of removal of up to 150 mg of iron per litre of culture vessel per hour have been obtained.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
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  • 72
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 41-50 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A method has been developed for the recovery of enterotoxin from bacterial culture supernatants of staphylococcus strain S-6 by use of the ion-exchange resin Amberlite XE-64 (IRC-50). The enterotoxin from 200 ml of bacterial culture supernatant diluted with one volume of water and adjusted to pH 6 can be removed with 1 g of resin pretreated with 0·02 M sodium phosphate, pH 6·2. The enterotoxin is recovered from the resin with 0·2 M sodium phosphate, pH 6·2, with subsequent precipitation with EtOH at -10°C, dialysis, and freeze drying. Partially purified preparations containing 1 emetic dose per 50μg were obtained.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
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  • 73
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 65-77 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Tomato puree, groundnut and soya protein, and sugar-gelatine solutions were successfully dried in a Scott pilot plant spray-drier. The tomato puree did not need the addition of a carrier provided that the air plenum of the drier was suitably modified. Spray-drying did not change the colour of tomato puree and the protein solutions, the viscosity characteristics of the protein solutions, or the nutritive value of the proteins. The conditions for the successful spray-drying of sugar-gelatine solutions were rather critical. The temperature in the spray-drier had the major effect on the quality of the powders. Published data on spray-drying do not provide a basis for predicting the drying behaviour of different products; experiments on the plant are essential.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
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  • 74
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 111-113 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 75
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 87-94 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: A laboratory spray drier with a gas flow of 2 to 3 ft3/min was used to dry suspensions containing spores of Bacillus anthracis or cells of Pasteurella tularensis. Dried material was collected in a 1/2-in. diameter cyclone that retained about 97 per cent of the product from the drier. The particle size distributions of the powders varied with the nature of substances in the suspension to be dried; as much as 80 per cent of the mass in particles 5 μ or less in diameter was obtained only when egg yolk was added to the suspensions. Retention of viability was about 90 per cent for B. anthracis spores and these powders contained from 175 to 522 × 109 viable spores/gram. Retention of viability of P. tularensis cells was 0·6 per cent or greater only when the suspension contained a relatively high proportion of stabilizers. With sucrose-skim milk-thiourea stabilizer about 10 per cent recovery of viability was obtained when the proportion of stabilizer solids to other solids was 3 : 1 or 6 : 1. These powders contained 140 × 109 viable cells per gram and the mass median diameter was 6·0 μ. The properties of the powders suggest that spray dried vaccines may be prepared for use either in suspension or in aerosols.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
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  • 76
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961) 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 77
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Biochemical and Microbiological Technology and Engineering 3 (1961), S. 277-309 
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Symptoms of oxygen deficiency and variations in dissolved-oxygen level have been described in some typical aerobic fermentations. In bacterial fermentations, dissolved-oxygen levels were almost the same in different parts of the fermentor. As a result, good correlations between apparent and true critical oxygen concentrations were obtained. Poor correlations were obtained in mould fermentations.
    Zusätzliches Material: 27 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 78
    ISSN: 0368-1467
    Schlagwort(e): Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: In the investigation of the redox potential changes of the oxidative fermentation of L-sorbose to 2-keto-L-gulonic acid, it was found that the 2-keto-L-gulonic acid formation proceeded favourably at low negative redox potential values. Low redox potentials were maintained in a continuous fermentation process in the chemostat, with a resulting high 2-keto-L-gulonic acid formation rate. L-Tyrosine and some of its analogues stimulated the 2-keto-L-gulonic acid formation. The stimulation seemed to be bound to low redox potential values, and suggested the action of a direct oxidase-type enzyme.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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