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  • Electronic Resource  (3)
  • 1990-1994  (3)
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  • Electronic Resource  (3)
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  • 1
    Electronic Resource
    Electronic Resource
    Fetal liver-cell transplantation for surgically-induced acute hepatic failure in rats (1993)
    Springer
    Pediatric surgery international 8 (1993), S. 23-26 
    Details
    ISSN: 1437-9813
    Keywords: Fetal liver cells ; Transplantation ; Acute hepatic failure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Pediatric liver transplantation has become increasingly successful, but donor scarcity is a major limitation. We studied fetal liver-cell transplantation as an alternative to provide functional hepatic replacement and evaluated the efficacy of the intraperitoneal (i.p.) transplantation of fetal liver cells in the treatment of acute hepatic failure in rats. Outbred Wistar rat fetuses (18–20 days' gestation) were used as donors. In Wistar male rats (250–300 g), acute hepatic failure was achieved by simultaneous portacaval shunt and 70% hepatectomy. This model produced lethal hepatic failure in a highly reproducible manner. Fetal liver cells were isolated by the mechanical method. Group A consisted of 2×107 fetal liver cells suspended in 1 ml phosphate-buffered saline (PBS) while group B consisted of only 1 ml PBS. Both were injected i.p. just after surgery. Fetal liver-cell transplantation (group A) significantly improved survival. The degree of hypoglycemia was significantly less significant 1 day after surgery in group A and the levels of plasma insulin and glucagon 3 days after surgery were significantly lower in group A than in group B. The results indicate that i.p. transplantation of fetal liver cells can provide metabolic support in rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02352995
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Immunological and virological characterization of improved construction of recombinant vaccinia virus expressing rinderpest virus hemagglutinin (1991)
    Springer
    Archives of virology 116 (1991), S. 81-90 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We constructed a recombinant vaccinia virus (RVV) expressing rinderpest virus (RPV) hemagglutinin (H) by modifying the promoter region of the original RVV. The promotor region was modified at three points, i.e., an outframe ATG was eliminated, the sequence between the promoter and initiation codon was shortened and the base sequence just upstream of the initiation codon was changed. As compared with the original RVV, the modified RVV was found to produce a remarkably large amount of H protein in infected rabbit kidney cells cultured in vitro and to induce high titers of anti-RPV-H antibodies in rabbits. The median protective doses in rabbits of the modified and of the original RVVs were 102 pfu and 103.5 pfu, respectively, indicating that the modified RVV was at least 10-times more effective in protection than the original. The neurovirulence of the modified RVV and the parental LC16mO strain was roughly at the same level, and was much lower than that of WR strain. The modified RVV was as heat-stable as the original one. These results indicate that the modified RVV could be a candidate rinderpest vaccine for further examinations including cattle.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01319233
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Severe pulmonary pneumocystosis in simian acquired immunodeficiency syndrome induced by simian immunodeficiency virus: its characterization by the polymerase-chain-reaction method and failure of experimental transmission to immunodeficient animals (1993)
    Springer
    Parasitology research 79 (1993), S. 624-628 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Pneumocystis carinii (Pc) infection was observed in three of five rhesus monkeys infected with simian immunodeficiency virus (SIVmac251). They showed severe symptoms similar to those associated with human acquired immunodeficiency syndrome (AIDS). Histopathology revealed severe pulmonary pneumocystosis in one of three Pc-positive monkeys, and anti-Pc antibodies were detected in sera from two of the three monkeys. Localization of Pc organisms in various organs of the monkeys was examined by the polymerase-chain-reaction (PCR) method, and Pc-specific bands of DNA amplification were detected in the liver, kidney, spleen, adrenal gland, testis, brain, and other organs examined, but no Pc organism was found in these organs by histopathologic examination. These results suggest that the activation of a latent infection of Pc occurs in SIV-infected rhesus monkeys as well as in human AIDS. Experimental transmission of Pc derived from a simian was attempted in severe combined immunodeficiency (SCID) mice and athymic nude (rnu/rnu, F344) rats. These animals were inoculated intranasally with 104 Pc cysts, but neither histopathologic changes nor Pc organisms were detected in SCID mice at 4 months after inoculation or in nude rats at 2 months postinoculation, suggesting that simian Pc is species-specific.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00932502
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    Paper (German National Licenses)
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