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  • 1
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Oranizational forms and the current status of genetic counseling within the health care system of 15 European countries were evaluated by questionnaire and at a symposium, with individuals present from Austria, Belgium, Czechoslovakia, Denmark, the Federal Republic of Germany, the German Democratic Republic, Finland, France, Hungary, Italy, the Netherlands, Norway, Switzerland, the United Kingdom, and the Soviet Union. In spite of wide differences between these countries, certain similarities with respect to the delivery of genetic counseling services could be observed: (i) most genetic counseling is done within university institutions or closely linked to it; (ii) governmental support of genetic counseling is developing slowly, and genetic counseling is usually not yet fully integrated into the health care system; (iii) there is lack of qualified personnel; (iv) no guide lines for formal education have been developed, but a postgraduate training period of no less than four years is considered a minimum; (v) without appropriate support, genetic counseling is a burden for research in human genetics; yet, a strict separation of genetic counseling and research activities is not recommended; (vi) on the average, a team providing genetic counseling for about 1–2 million people should consist of 3–4 physicians, 5–10 technicians, 2–3 secretaries, and other supportive personnel.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 58 (1980), S. 43-45 
    ISSN: 1432-1440
    Keywords: Glucocorticoid-Rezeptoren ; Leukämien ; Sensitivität ; Glucocorticoid receptors ; Leukemias ; Sensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Determination of steroid receptors has been used to predict steroid sensitivity in various neoplasias. In an attempt to investigate its applicability in human leukemias we have studied glucocorticoid receptors in the leukemic cells from 23 patients with various hematologic neoplasias and in the lymphocytes from 18 normal donors. Specific glucocorticoid binding in intact cells was determined by a whole cell competitive binding assay. Normal lymphocytes have about 4,611 specific binding sites per cell. The blasts from 9 patients with acute myelogenous leukemias (AML) have strongly varying high levels of specific binding sites, ranging from 4,817 to 15,416 per cell. Of the 13 patients with chronic lymphocytic leukemia (CLL), 5 have received glucocorticoid treatment for years and were clinically resistant to glucocorticoid. Their lymphocytes have lower specific binding sites (range: 2,047 to 3,999) than the other CLL cases which were newly diagnosed (range: 3,734 to 11,020). Our results suggest that determination of glucocorticoid receptors might be of value in predicting clinical responsiveness in leukemias.
    Notes: Zusammenfassung Bei einigen steroid-sensitiven Neoplasien ist die Bestimmung von Steroid-Rezeptoren für die Voraussage einer Ansprechbarkeit auf die Hormontherapie von Bedeutung. Um die Anwendung dieser Methode auch bei Leukämien nachzuprüfen, haben wir die Glucocorticoid-Rezeptoren in Leukämiezellen von 23 Patienten und in Lymphozyten von 18 Kontrollpersonen untersucht. Die Bestimmung erfolgte nach einer Methode nach Baxter und Tomkins. Normallymphozyten haben circa 4611 spezifische Bindungsstellen pro Zelle. Der Anzahl der Steroid-Rezeptoren in den Blasten von 9 Patienten mit AML schwankten stark (Bereich: 4817–15416 Stellen/Zelle). Fünf von 13 Patienten mit CLL wurden seit Jahren mit Glucocorticoid behandelt und sprachen zur Zeit der Rezeptoren-Bestimmung auf die Therapie nicht mehr an. Die Anzahl der Bindungsstellen der Lymphozyten dieser Patienten war im Mittel niedriger (Bereich: 2047–3999), als bei den Patienten, die neu diagnostiziert worden waren (Bereich: 3734–11020). Unsere Ergebnisse weisen darauf hin, daß die Bestimmung von Steroid-Rezeptoren für die Planung einer Therapie mit Glucocorticoiden von Bedeutung sein könnte.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1618-2650
    Keywords: Elektroden, ionenselektive ; Reproduzierbarkeit, Zweipunkteichung, Diskelektroden
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Für die Na+-, K+- und Ca2+ -Bestimmung mit ionenselektiven Diskelektroden im medizinischen Bereich empfiehlt sich die Zweipunkteichung. Die mit diesem Verfahren erzielbare Reproduzierbarkeit wird theoretisch aufgrund des Ablesefehlers am Meßinstrument berechnet und mit experimentellen Werten verglichen. Die theoretisch berechneten und experimentellen Standardabweichungen liegen für 1-wertige Ionen bei 0,3 % und für 2-wertige bei 0,6 %. Die Genauigkeit wird nicht durch die Elektroden, sondern die gegenwärtig verfügbaren Meßgeräte begrenzt.
    Notes: Summary In clinical analysis it is recommendable to use a two-point calibration for the determination of Na+, K+ and Ca2+ ions by ion-selective disc electrodes. The accuracy, which can be obtained by this calibration method, is treated theoretically, and the results are compared with experimental data. Theoretical and experimental standard deviations are in very good agreement, with 0.3 % for Na+ and K+ and 0.6 % for Ca2+. The precision is limited by the measuring instrument and not by the disc electrodes.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 301 (1980), S. 410-413 
    ISSN: 1618-2650
    Keywords: Elektroden, ionenselektive ; Diskelektroden, strömungsoptimiertes Multimeßsystem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es wird ein strömungsoptimiertes Multimeßsystem mit ionenselektiven Diskelektroden vorgestellt, bei dem aufgrund stromlinienförmiger Erhebungen im Kanalsystem gegenüber den Carrier-PVC-Membranen in Verbindung mit einem stau- und totzonenarmen Strömungsverlauf eine trägheitsarme und präzise ionenselektive Analytik gewährleistet wird. In das Meßsystem lassen sich auch andere Sensoren wie pH-, gassensitive und Enzym-Elektroden einführen.
    Notes: Summary The proposed system ensures low-inertia and precise measurements by means of ion-selective disc electrodes. This is achieved by streamlined elevations in the channel system opposite to the carrier PVC membranes, caring for a flow low in stagnation and dead zones. Other sensors may also be used in this system, as e.g. pH-, gas-sensitive and enzyme electrodes.
    Type of Medium: Electronic Resource
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