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  • 1
    Electronic Resource
    Electronic Resource
    Erratum: Two- and three-body forces in the interaction of He atoms with Xe overlayers adsorbed on (0001) graphite [J. Chem. Phys. 91, 6477 (1989)] (1990)
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 93 (1990), S. 4492-4492 
    Details
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.459724
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  • 2
    Electronic Resource
    Electronic Resource
    Differential Activation of Monocytes in Haemodialysis Patients Exposed to Different Types of Membranes (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 31 (1990), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Peripheral blood mononuclear cells (PBMC) from haemodialysis patients treated with different types of membranes were isolated, incubated in vitro, and extracellular and cell-associated interleukin 1 (IL-1) assayed by radioimmunological methods. Extracellular IL-1 was low and not different from controls, regardless of the dialyser used. In contrast, cell-associated IL-1 was increased in patients treated with dialysers containing low-Hux Cuprophan (CU, n = 5) and polyacrylonitrile sheet membrane (AN69, n = 5), Patients treated with diaiysers containing highflux polysulphone (PS, n= 7), and polymethylmethacrylate (PMMA, n= S), exhibited no increase in cell-associated IL-l under these conditions. To elucidate the mechanism of the activation, aqueous extracts of dialysers containing CU, PS, and AN69 were tested for their ability to induce IL-1 generation in PBMC from healthy donors. Extracts from unrinsed CU-containing dialysers caused significant IL-1 synthesis and release, whereas incubation with extracts from dialysers containing PS and AN69 sheet membranes did not. Hence, although both CU and AN69 sheet type dialysers result in activation of blood monocytes the mechanism of action appears to be different. We speculate that functional signs of PBMC activation as evidenced by increased spontaneous IL-1 production observed in some patients on long-term haemodialysis may result from extractable dialyser membrane material while in other instances direct cell membrane interactions or endotoxin transfer from the dialysate may be relevant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1990.tb02758.x
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  • 3
    Electronic Resource
    Electronic Resource
    Indications for radioisotope bone scanning in staging of cervical cancer (1990)
    Springer
    Archives of gynecology and obstetrics 248 (1990), S. 21-23 
    Details
    ISSN: 1432-0711
    Keywords: Radioisotope bone scanning ; Staging examinations ; Cervical cancer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The total of 160 patients with newly diagnosed invasive cancer of the cervix had whole body radioisotope bone scanning during staging of their disease. 51 patients had cancer of the cervix stage I, 63 had stage II, 34 stage III and 12 stage IV (FIGO). Only in 8 of 160 patients did the bone scans indicate possible metastases and this was confirmed by X-ray examination in only one patient with stage IV disease and liver metastases. We conclude that patients with stage I and stage II carcinoma of the cervix do not need to have bone scans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02389585
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  • 4
    Electronic Resource
    Electronic Resource
    Desmin and actin associated with cytoplasmic bodies in skeletal muscle fibers: immunocytochemical and fine structural studies, with a note on unusual 18- to 20-nm filaments (1990)
    Springer
    Acta neuropathologica 80 (1990), S. 406-414 
    Details
    ISSN: 1432-0533
    Keywords: Cytoplasmic body myopathy ; Immunohistochemistry ; Desmin ; Intermediate filaments ; Actin filaments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In a fine structural and immunocytochemical study, the latter performed on semithin sections of epoxy resin embedded skeletal muscle fibers, three types of cytoplasmic bodies were identified in a case of cytoplasmic body myopathy: (1) The first type, the classical type, showed a central core and a light halo with radiating actin filaments at the periphery. (2) The second type, the spheroid body was characterized by irregularly arranged granular masses associated with intermediate filaments. Desmin immunoreactivity occurred in the central and peripheral parts, where filaments of intermediate size were visualized by electron microscopy. Desmin immunoreactivity was noted also at the Z-bands of striated annulets, within areas of disordered myofibrils, such as sarcoplasmic masses, and in atrophic muscle fibers. (3) The third type of the cytoplasmic body was composed mainly of large masses of uneven granularity and electron density. The center of this type reacted to anti-actin antibody suggesting that the 5- to 6-nm filaments, which ultrastructurally proved to be a major component, were of the actin type. By contrast, neither intermediate filaments nor actin microfilaments were found by electron microscopy in cytoplasmic bodies in a second case where no immunoreaction to desmin or actin occurred. Anti-vimentin antibody stained only the cytoplasm of endomysial cells, but not the inclusion bodies. Some other, unusual inclusions with 18- to 20-nm tubulo-filamentous structures have to be distinguished from the various types of filaments in cytoplasmic bodies. It is concluded, that pleomorphism and heterogeneity of “cytoplasmic bodies” have to be taken into consideration when classifying cytoplasmic body myopathies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307695
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  • 5
    Electronic Resource
    Electronic Resource
    Neuroendocrine effects of ipsapirone on the hypothalamic-pituitary adrenal axis: CRF, ACTH and cortisol in healthy volunteers (1992)
    Springer
    European journal of clinical pharmacology 42 (1992), S. 163-169 
    Details
    ISSN: 1432-1041
    Keywords: Ipsapirone ; CRF ; ACTH ; cortisol ; time series analysis ; hypothalamic-pituitary-adrenal axis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Summary The neuroendocrine effects (changes in plasma CRF, ACTH and cortisol) of single and multiple (t.d.s. for 2 days) doses of ipsapirone (BAY Q 7821) 5 and 10 mg have been investigated in 6 healthy male volunteers. The study followed a balanced complete block, placebo-controlled and double blind design with two baseline phases (pre and post-treatment). Volunteers were investigated on identical days during 5 successive weeks. The results do not show a specific effect of ipsapirone on the hypothalamic-pituitary-adrenal axis when doses in the range of 5–30 mg per day were given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00278478
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  • 6
    Electronic Resource
    Electronic Resource
    Purification and properties of a F420-nonreactive, membrane-bound hydrogenase from Methanosarcina strain Gö1 (1992)
    Springer
    Archives of microbiology 157 (1992), S. 505-511 
    Details
    ISSN: 1432-072X
    Keywords: Archaebacteria ; methanogenesis ; energy conservation ; membranes ; F420-dependent hydrogenase ; Methanosarcina
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of the F420-reactive and F420-nonreactive hydrogenases from the methylotrophic Methanosarcina strain Gö1 indicated a membrane association of the F420-nonreactive enzyme. The membrane-bound F420-nonreactive hydrogenase was purified 42-fold to electrophoretic homogeneity with a yield of 26.7%. The enzyme had a specific activity of 359 μmol H2 oxidized · min-1 · mg protein-1. The purification procedure involved dispersion of the membrane fraction with the detergent Chaps followed by anion exchange, hydrophobic and hydroxylapatite chromatography. The aerobically prepared enzyme had to be reactivated anaerobically. Maximal activity was observed at 80°C. The molecular mass as determined by native gel electrophoresis and gel filtration was 77000 and 79000, respectively. SDS gel electrophoresis revealed two polypeptides with molecular masses of 60000 and 40000 indicating a 1:1 stoichiometry. The purified enzyme contained 13.3 mol S2-, 15.1 mol Fe and 0.8 mol Ni/mol enzyme. Flavins were not detected. The amino acid sequence of the N-termini of the subunits showed a higher degree of homology to cubacterial uptake-hydrogenases than to F420-dependent hydrogenases from other methanogenic bacteria. The physiological function of the F420-nonreactive hydrogenase from Methanosarcina strain Gö1 is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276770
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  • 7
    Electronic Resource
    Electronic Resource
    A fluorescence detection system for the analytical ultracentrifuge and its application to proteins, nucleic acids, and viruses (1990)
    Springer
    Colloid & polymer science 268 (1990), S. 45-54 
    Details
    ISSN: 1435-1536
    Keywords: Analyticalultracentrifuge ; fluorescencedetection ; proteins ; nucleicacids ; viruses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A fluorescence detection system was developed for the analytical ultracentrifuge Spinco model E. Fluorescence is excited by a laser beam which is focussed into the cell and illuminates an area with a dimension of 60 μm in radial direction. For scanning the laser beam is moved in radial direction. After passing the cell, the laser beam is quenched by a carbon light trap and a set of optical filters. Fluorescence emission intensity is monitored by a photomultiplier located behind the light trap and the set of filters. The sensitivity of the detection system was tested by applying it to the sedimentation analysis of proteins and nucleic acids. Bovine serum albumin (BSA) was covalently labelled with the fluorescence-dye fluorescein-isothiocyanate (FITC), and its sedimentation coefficient could be determined even if BSA was analyzed in a concentration as low as 10−10 M. Nucleic acids were labelled non-covalently by the intercalating dye ethidium bromide. Only 8 ng RNA were needed for the determination of the sedimentation coefficient. The particular advantages of the fluorescence detection system were exploited for the establishment of a new method for quantitative virus detection. To tobacco mosaic virus (TMV) a monoclonal anti-TMV antibody from mouse was bound, and to this a second, anti-mouse antibody that carried the fluorescence-label FITC was attached. Either by UV-irradiation or by incubation with glutaraldehyde, the first antibody was covalently crosslinked to TMV, and the second antibody to the first. In CsCl density centrifugation with fluorescence detection as little as 3.2 ng virus/80 μl or 6×108 virus particles/ml were recorded in a well expressed band at the corresponding buoyant density. Tenfold lower concentration would result still in a significant band. The sensitivity compares well with those of the most advanced techniques from immunology. Due to the specific labelling of viruses by antibodies it will be possible to carry out quantitative physical characterization of virus containing samples without purifying the virus. Future applications of the fluorescence detection system and of the virus detection technique are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01410422
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  • 8
    Electronic Resource
    Electronic Resource
    The structure of C2H4 clusters from theoretical interaction potentials and vibrational predissociation data (1990)
    Springer
    The European physical journal 15 (1990), S. 341-351 
    Details
    ISSN: 1434-6079
    Keywords: 36.40.01 ; 33.80.Gj
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Optimized geometries and binding energies are calculated for ethene (ethylene) dimers, trimers, and tetramers based on a pairwise additive dimer potential. From these results intermolecular frequencies and relative abundancies (catchment areas) of the different isomers are obtained and compared with the results of accurate measurements of the photodissociation upon absorption of one photon of a CO2 laser in the region of thev 7 monomer absorption band at 949 cm−1. The clusters are size selected in a scattering experiment and show for a cluster size fromn=2 ton=6 a frequency maximum shifted by 3 cm−1 to the blue compared with the monomer. The result is explained by the predominance of chains and chain-like structures of the clusters in the photodissociation process. The chains consist of cross-like dimer sub-units.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01437178
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  • 9
    Electronic Resource
    Electronic Resource
    Funktionelle Biochemie des Menschen. Band 1 und 2. (Reihe: Wissenschaftliche Taschenbücher.) Von E. Hofmann. Akademie Verlag, Berlin. Band 1: 1990, 316 S., Broschur DM 16.00.  -  ISBN 3-05-500626-7; Band 2: 1991, 402 S., Broschur DM 29.80.  -  ISBN 3-05-500627-5 (1992)
    Weinheim : Wiley-Blackwell
    Zeitschrift für die chemische Industrie 104 (1992), S. 494-495 
    Details
    ISSN: 0044-8249
    Keywords: Chemistry ; General Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ange.19921040438
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  • 10
    Electronic Resource
    Electronic Resource
    Die charakterisierung der porosität von polyacrylnitril-faserstoffen im gelzustand (1992)
    Weinheim : Wiley-Blackwell
    Acta Polymerica 43 (1992), S. 151-158 
    Details
    ISSN: 0323-7648
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Measurement methods that are applicable for the characterization of porous structures, i.e. differential scanning calorimetry, low-temperature gas adsorption, mercury high pressure porosimetry, electron and light microscopy, X-ray small angle scattering, water retention value, and apparent density, are described with respect of the peculiarities of poly(acrylonitrile) fibres. The methods have been applied on differently stretched and thermally treated PAN fibres in the gel state and after dehydration, respectively. The observed effects are explained by the different stabilities of the gel fibre structure.
    Notes: Die zur Charakterisierung von Porenstrukturen anwendbaren Meßmethoden Differential-Scanning-Calorimetrie, Tieftemperatur-Gasadsorption, Quecksilber-Hochdruckporosimetrie, Elektronen- und Lichtmikroskopie, Röntgenkleinwinkelstreuung sowie Wasserrückhaltevermögen und scheinbare Dichte werden unter Berücksichtigung der Besonderheiten von Polyacrylnitrilfasern vorgestellt. Die genannten Methoden wurden auf unterschiedlich gereckte und thermisch behandelte PAN-Fasern im Gelzustand und nach Entwässerung angewendet. Die dabei auftretenden Effekte werden mit der unterschiedlichen Stabilität der Gelfaserstruktur erklärt.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/actp.1992.010430305
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