Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Na-Rückresorption und O2-Verbrauch der Niere (1960)
    Springer
    Journal of molecular medicine 38 (1960), S. 680-680 
    Details
    ISSN: 1432-1440
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01486946
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Die renale Behandlung des Alkohols und dessen tubuläre Wirkung (1985)
    Springer
    Journal of molecular medicine 63 (1985), S. 944-947 
    Details
    ISSN: 1432-1440
    Keywords: Alcohol ; Ethanol ; Acetaldehyde ; Alcohol dehydrogenase ; Kidney ; Renal tubule ; Alkohol ; Äthanol ; Acetaldehyd ; Alkoholdehydrogenase ; Niere ; Nierentubulus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die renale Behandlung des Äthanols umfaßt sowohl die glomeruläre Filtration als auch die tubuläre Reabsorption. Wegen seiner hohen Permeabilität nähert sich die Konzentration des Alkohols in der Tubulusflüssigkeit derjenigen der peritubulären Flüssigkeit an, und unter Steady-state-Bedingungen ist die Alkoholkonzentration im Endharn fast genau so hoch wie im Serumwasser. Selbst hohe Alkoholkonzentrationen stören die Nierenzellfunktion nicht nennenswert. Das scheint darauf zu beruhen, daß Nierengewebe praktisch keine Alkoholdehydrogenase enthält. Deswegen sammelt sich Acetaldehyd, das zytotoxische Stoffwechselprodukt des Alkohols, nicht in wirksamer Dosis an. Nach direkter Zufuhr im Rahmen von Mikropunktionsexperimenten übt Alkohol keine nennenswerten Wirkungen aus. Demgegenüber blockiert Acetaldehyd die wesentlichen Parameter der Zellvitalität, gemessen in Form der elektrischen Membranpotentiale und der intrazellulären Ionenaktivitäten.
    Notes: Summary The renal handling of ethanol comprises glomerular filtration and tubular reabsorption. Due to its high permeability alcohol concentration in the tubular fluid approaches that of peritubular fluid and under steady state conditions alcohol concentration in the final urine is almost the same as in serum water. Even in high concentrations alcohol does not significantly interfere with kidney cell function. This seems to be due to the fact that renal tissue is almost free from alcohol dehydrogenase. Thus, acetaldehyde, the cytotoxic intermediate of alcohol metabolism, is not accumulated in effective dosis. If applied directly in micropuncture experiments alcohol is without distinct effects while acetaldehyde inhibits the main parameters of cellular vitality as measured by electrical membrane potentials and intracellular ion activities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01738149
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Damaging effects of high energy shock waves on cultured Madin Darby Canine Kidney (MDCK) cells (1990)
    Springer
    Urological research 18 (1990), S. 255-258 
    Details
    ISSN: 1434-0879
    Keywords: ESWL ; Shock waves ; MDCK cells ; LDH ; GOT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Shock wave lithotripsy (ESWL) has become an almost non-invasive standard treatment modality for urolithiasis. Several investigations, however, demonstrated that ESWL is not completely free of side effects. Among others alteration of renal tubular function has been reported. To study the effect of shock waves on tubular cells directly an in-vitro model with cultured Madin Darby Canine Kidney (MDCK) cells was established. Suspensions of MDCK cells (7 groups of 6 containers each) were exposed to 0, 16, 32, 64, 128, 256 shock waves (Dornier HM4, 18 kV). Before and 0, 1, 3, 6, 9, 12, 24 h after ESWL the following parameters were measured in the nutrient medium: lactate dehydroxygenase (LDH), glutamate oxalacetate transaminase (GOT), electrolytes. LDH and GOT increased depending on the number of shock waves indicating a membrane damage of MDCK cells. The MDCK model seems suitable for further studies on the effect of shock waves on renal tubular cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00294768
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Renal handling of urate and oxalate: Possible implications for urolithiasis (1979)
    Springer
    Urological research 7 (1979), S. 143-148 
    Details
    ISSN: 1434-0879
    Keywords: Urate ; Oxalate ; Renal Handling ; Precipitation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Both urate and oxalate are organic acids of considerable clinical interest, owing to their limited solubility. Calcium oxalate is the most frequent constituent of renal calculi and occasionally precipitates in body fluids. Urate precipitations are common in the kidney and in various other tissues. In this paper, a short outline of the present knowledge of renal handling of these substances will be followed by some conclusions as to the possible relevance of this knowledge for the understanding of urolithiasis and intrarenal precipitation. Direct (micropuncture) data are available for urate in the rat (1,6, 7, 10, 21, 23, 28, 36, 42), rabbit (35), dog (34) and cebus monkey (33) and in the rat only for oxalate (11, 15, 20).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00257198
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Handling of uric acid by the rat kidney (1972)
    Springer
    Pflügers Archiv 335 (1972), S. 257-265 
    Details
    ISSN: 1432-2013
    Keywords: Uric Acid Reabsorption ; Uric Acid Secretion ; Microperfusion ; Proximal Tubule of the Rat Kidney
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Under free flow conditions there is a net transport of uric acid in the rat proximal tubule. This net transport is the result of two unidirectional fluxes which in the range of normal plasma concentrations of uric acid follow a first order kinetic, i.e. the unidirectional fluxes are in linear proportion to uric acid concentration in tubular fluid or in plasma, respectively. The transport coefficient of the influx (in secretion direction) exceeds that of the outflux (in reabsorption direction) by a factor of 2.5.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00586216
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Handling of allantoin by the rat kidney (1975)
    Springer
    Pflügers Archiv 357 (1975), S. 201-207 
    Details
    ISSN: 1432-2013
    Keywords: Allantoin ; Uricase ; Kidney ; Clearance ; Micropuncture ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Renal excretion of allantoin was measured by tracer techniques. After injection of 2-C14 urate and H3 inulin, clearances of allantoin and inulin were measured and both proximal and distal tubules were micropunctured. In confirmation of earlier results 2-C14 urate injected into an intact animal is very rapidly converted to C14 allantoin: after 15 min more than 90% of urinary tracer is present as allantoin. It was further observed that 1) allantoin clearance is essentially identical with inulin clearance over a wide range of urine flows; 2) no net transport of allantoin occurs in either proximal or distal tubules. Clearly allantoin is handled by the rat kidney like inulin. The total excretion of filtered allantoin unlike that of filtered urate provides an easy and effective mechanism for animals possessing the enzyme uricase to dispose of their purine loads.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00585975
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Handling of oxalate by the rat kidney (1978)
    Springer
    Pflügers Archiv 374 (1978), S. 243-248 
    Details
    ISSN: 1432-2013
    Keywords: Oxalate ; Wistar rat ; Microperfusion ; Microinfusion ; Organic acid secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Renal transport of14C-oxalate was studied in the rat by clearance and micropuncture techniques. The ultrafilterability of oxalate was 0.98±0.02 (n=7). Fractional clearance of oxalate was significantly above unity in antidiuresis and volume expansion: mean 1.24 ±0.04 (n=115). Pyrazinamide (1.1·10−3 mol/kg BW) and probenecid (0.35·10−3 mol/kg BW) had no significant effect on oxalate clearance. P-aminohippurate (1.45·10−3 mol/kg BW) and urate (0.48 ·10−3 mol/kg BW) depressed the fractional clearance of oxalate significantly from 116 to 91 and from 125 to 90%, respectively. Excess excretion of14C-oxalate over3H-inulin was invariably demonstrable in peritubular microperfusion experiments (n=5) and in microinfusions underneath the kidney capsule (n=4). Together with the first 50% of3H-inulin 58±2% of the total14C-oxalate were excreted in the peritubular microperfusions, and 64±3% in the subcapsular microinfusions. In tubular microinfusion experiments (n=36) urinary14C-oxalate recovery was almost complete after early proximal microinfusion (93±4%) and complete after late proximal microinfusion (102±4%). In continuous microperfusion experiments of proximal tubules (n=42) a small but highly significant outflux of14C-oxalate of 7% per mm perfusion distance was found. The data suggest that oxalate is freely filterable at the glomerular site. A small but significant amount of oxalate is reabsorbed in the proximal nephron. Most likely at the same site and in the pars recta oxalate is secreted and tubular load increased to 124% of filtered load. This amount is excreted in final urine. The secretion of oxalate is inhibited by organic acids which are known to be secreted by the proximal tubule and the pars recta.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00585601
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    In vivo studies on uricase activity in the rat (1974)
    Springer
    Pflügers Archiv 351 (1974), S. 323-330 
    Details
    ISSN: 1432-2013
    Keywords: Uricase ; Urate ; Allantoin ; Liver ; Kidney ; Microperfusion ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. In vivo uricase activity was tested in rats by injection of 2-C14 urate and measurement of the total C14 activity and the fractional activities of allantoin, allantoic acid and urea in samples of blood and urine. In control animals, 5 min after the injection, 70% of the plasma tracer was already present in the form of allantoin. No allantoic acid and urea were produced. Intestinectomy had no measurable influence on uricase activity. On the other hand, hepatectomy or ligation of the hepatic artery combined with subtotal viscerectomy did abolish uricase activity almost completely. 2. Following microinjections into proximal tubules of Ringer solution containing 2-C14 urate, urine samples during early recovery mainly contained labelled urate, whereas in later samples the fraction of labelled allantoin increased. About 12 min after the microinjection the urine of both kidneys contained equal amounts of tracer mainly in the form of allantoin. 3. When segments of proximal tubules were perfused with an equilibrium solution containing tracer amounts of C 14 urate, no urate was metabolized during its passage through the proximal tubule. 4. C 14 urate was offered from the peritubular capillaries and samples of tubular fluid were analyzed, Again, all the tracer in the tubular fluid was in the form of urate, indicating that urate is not oxidized when it is transported across the tubular cell. It is concluded from these results that: 1. The rat kidney has no significant uricase activity. 2. Urate transport in the kidney is not influenced by this enzyme. 3. The degradation of urate to allantoin takes place at extrarenal sites, mainly in the liver.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00593318
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Maleic acid induced aminoaciduria, studied by free flow micropuncture and continuous microperfusion (1979)
    Springer
    Pflügers Archiv 382 (1979), S. 109-114 
    Details
    ISSN: 1432-2013
    Keywords: Renal tubule ; Microperfusion ; Maleic acid ; Amino acid transport ; Fanconi syndrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The injection of 200 mg/kg BW maleic acid was found to be a suitable dose for exploring the experimental Fanconi syndrome by micropuncture techniques in rats. In clearance experiments, the fractional excretion of glycine,l-alanine,l-aspartate and taurine was measured. After intraperitoneal administration of maleic acid the excretion of these amino acids was increased in the range between the 20-fold and the 230-fold. Free flow micropuncture experiments showed that the reabsorption of these amino acids is reduced drastically along the whole proximal tubule. Continuous microperfusion experiments lead to the result that, in maleic acid pretreated rats, the reabsorption of14C-glycine from the proximal convolution was strongly inhibited. It was found, furthermore, that after blocking the saturable glycine transport byl-phenylalanine, the remaining reabsorption of glycine (corresponding to passive diffusion) was exactly the same with and without maleic acid. Microinfusion experiments with 8 μmol·l−1 l-3H-alanine into the early distal tubule showed a fractional recovery of 103±4.2% (S.D.) in the control and of 101±6.5% in presence of maleic acid. It is concluded that maleic acid inhibits the saturable reabsorption mechanism of amino acids along the proximal tubule. Passive permeability of the tubular membrane does not seem to be altered by maleic acid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00584210
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Kinetics ofl-proline reabsorption in rat kidney studied by continuous microperfusion (1979)
    Springer
    Pflügers Archiv 382 (1979), S. 115-121 
    Details
    ISSN: 1432-2013
    Keywords: l-proline reabsorption ; Renal tubule ; Microperfusion ; Transport kinetics ; Diisopropylphosphorofluoridate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Renal tubular reabsorption of3H and14C labelledl-proline was measured in vivo et situ by continuous microperfusion of single proximal tubules of the rat. The reabsorption is shown to be saturable. Passive diffusion plays a relatively small role in the reabsorption. A maximum possible permeability coefficient of 25 μm2·s−1 for proline was calculated. Two transport systems were found, one with a small affinity and a high capacity, the other with a very high affinity and a small capacity. The following values were estimated: $$\begin{gathered} J_{\max 1} = {\text{ }}2.6 \pm 0.28{\text{ }}(SEM){\text{ }}nmol \cdot m^{ - 1} \cdot s^{ - 1} \hfill \\ K_{m1} {\text{ }} = {\text{ }}11.8 \pm 1.7{\text{ }}(SEM){\text{ }}nmol \cdot 1^{ - 1} \hfill \\ J_{\max 2} = {\text{ }}9.6 \pm 1.92{\text{ }}(SEM){\text{ }}pmol \cdot m^{ - 1} \cdot s^{ - 1} \hfill \\ K_{m2} {\text{ }} = {\text{ }}29.3 \pm 7.8{\text{ }}(SEM){\text{ }}\mu mol \cdot l^{ - 1} \hfill \\ \end{gathered} $$ Whereas the first system reabsorbs the bulk of the filtered load, the activity of the second system explains the extremely small amount of proline found in the final urine. Diisopropylphosphorofluoridate — a specific inhibitor of dipeptidyl peptidase IV — decreases the reabsorption ofl-proline andl-alanine but has no influence on the reabsorption of the basic amino acidl-arginine and the acidic amino acidl-glutamic acid. This result correlates with a recent speculation that dipeptidyl peptidase IV is involved in proline and alanine reabsorption.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00584211
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...