ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract— A simple fluorometric method for the estimation of endogenous 3,4-dihydroxyphenylethylene glycol sulphate (DOPEG-SO4) in brain tissue is described. DOPEG-SO4 was extracted from brain tissue with 0.4 n-perchloric acid and purified by column chromatography on QAE-Sephadex A-25, Cl− form. The isolated DOPEG-SO4 was hydrolysed by incubation with a purified preparation of sulphatase from Helix pomatia. The free DOPEG thus formed was further purified on a column of aluminium oxide and determined fluorometrically after conversion to a fluorescent compound by condensation with ethylenediamine.The sensitivity of this method was about 15 ng per perchloric acid extract applied on the column (3 ml) and the reproducibility was excellent, with a variation coefficient of 1.6% (n = 5). The concentration of DOPEG-SO4 in whole brains of rats, determined by the present method, was 79.3 ± 4.7 ng/g (mean ± S.D., n = 10) in terms of free DOPEG. No appreciable DOPEG-SO, was detected in the brains of mice and guinea-pigs.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1978.tb10488.x
Permalink