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  • Immunofluorescence microscopy  (2)
  • Monoclonal antibodies  (2)
  • (Sub)periosteal  (1)
  • Aneurysmal bone cyst  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Biologic characterization of human bone tumors (1983)
    Springer
    Journal of cancer research and clinical oncology 106 (1983), S. 234-239 
    Details
    ISSN: 1432-1335
    Keywords: Osteosarcoma ; Collagen types ; Immunofluorescence microscopy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Sixteen cases of typical highly malignant osteosarcoma were investigated by light, electron, and immunofluorescence microscopy to demonstrate the presence of collagen types I–III. It was shown that, in light-microscopically anaplastic areas of the tumor, collagen type III predominates, while only very few membranes of collagen type I are observed. Ultrastructurally, the cells are characterized by numerous free ribosomes in their cytoplasm and only a few membranes of granular endoplasmic reticulum (ER). In osteoblastic areas, collagen type I is increased, while type-III collagen is decreased. The cytoplasm of cells contains markedly more granular ER. An increasing mineralization of matrix is observed. In fibroblastic areas of the tumors, collagen types I and III are codistributed. Tumor cells have a fibroblast appearance with elongated nuclei and well developed granular ER. The chondroblastic areas, characterized by immature neoplastic cartilage, contain varying amounts of collagen type II. Chondroblast-like tumor cells have typical ring-shaped membranes of granular ER in their cytoplasm. The evidence of different collagen types in osteosarcomas lends additional support to the concept that a pluripotent mesenchymal cell is the stem cell of osteosarcomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402614
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  • 2
    Electronic Resource
    Electronic Resource
    Biologic characterization of human bone tumors I. Ewing's sarcoma (1982)
    Springer
    Journal of cancer research and clinical oncology 104 (1982), S. 171-180 
    Details
    ISSN: 1432-1335
    Keywords: Ewing's sarcoma ; Type IV collagen ; Factor-VIII-associated protein ; Endothelial differentiation ; Electron microscopy ; Immunofluorescence microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Six cases of Ewing's sarcoma were investigated by electron and immunofluorescence microscopy. A layer of basement membrane-like deposits was found between typical principal and secondary tumor cells. To clarify the nature of these ultrastructural deposits, antibodies against collagen type IV were applied to frozen sections of corresponding tumor tissue. This reaction revealed type IV collagen as a regular component of basement membranes in nonneoplastic tumor capillaries, but it was equally able to localize collagen type IV between single tumor cells in capillary-free areas. With the same method, factor-VIII-associated protein, predominantly found in endothelial cells, could be demonstrated in some tumor cells. These results demonstrate that, in addition to anaplastic cells, some tumor cells are found in Ewing's sarcoma that share certain differentiating features with the endothelial cell.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402065
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    High malignant surface osteosarcoma arising at the site of a previously treated aneurysmal bone cyst (1993)
    Springer
    Journal of cancer research and clinical oncology 119 (1993), S. 375-378 
    Details
    ISSN: 1432-1335
    Keywords: Malignant transformation ; Differential diagnosis ; Osteosarcoma ; Aneurysmal bone cyst
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A patient who developed a high malignant surface osteosarcoma at the site of a previously treated aneurysmal bone cyst is reported. The patient developed the osteosarcoma 4 years after complete curettage and bone-grafting of the cyst. The clinical, radiological and light microscopic features of this case are described. A causal relationship between the preexisting aneurysmal bone cyst and osteosarcoma is discussed, but seems to be unlikely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01218416
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  • 4
    Electronic Resource
    Electronic Resource
    (Sub)periosteal Ewing's sarcoma of bone (1992)
    Springer
    Journal of cancer research and clinical oncology 118 (1992), S. 72-74 
    Details
    ISSN: 1432-1335
    Keywords: Ewing's sarcoma ; (Sub)periosteal ; Differential diagnosis ; Prognostic factors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ewing's sarcoma is a small malignant round-cell tumour that arises from mesenchymal cells, predominantly in the medullary cavity of bone. In exceptional cases it originates in the soft tissues and subsequently invades the underlying bone. A (sub)periosteal origin of Ewing's sarcoma is a very rare condition: only a few cases have been published so far. Three cases of (sub)periosteal Ewing's sarcoma, having received neoadjuvant chemotherapy and radiation therapy as well as wide excision, are reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01192315
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  • 5
    Electronic Resource
    Electronic Resource
    Biological characterization of human bone tumors (1987)
    Springer
    Journal of cancer research and clinical oncology 113 (1987), S. 559-562 
    Details
    ISSN: 1432-1335
    Keywords: Immunohistological techniques ; Monoclonal antibodies ; Mononuclear phagocyte system ; Malignant fibrous histiocytoma ; Giant cell tumor of bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Seven giant cell tumors of bone and four malignant fibrous histiocytomas were studied immunohistochemically with different monoclonal antibodies to the mononuclear phagocyte system (MPS), to HLA-DR antigens, and to a proliferation-associated nuclear antigen (KI-67), in order to clarify the role of macrophages in these tumors. A part of the mononuclear cells stained positive with antibodies against the MPS. Antibody 25-F-9 against mature tissue macrophages showed the strongest reaction. The osteoclast-like giant cells also stained positive with this antibody. Fibroblast-like stromal cells, however, showed negative reactions to all antibodies against MPS cells. A double-labeling immunohistological technique was used to detect the proliferating cell population in these tumors. The fibroblast-like cells that were negative for MPS markers, were positively labeled with the monoclonal antibody Ki-67 against a proliferation-associated nuclear antigen, whereas a negative reaction to Ki-67 was seen in cells positive with antibodies to the MPS. These results support the concept that macrophages are a reactive population in these tumors, whereas the fibroblast-like mesenchymal cells are the proliferating tumor cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390865
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  • 6
    Electronic Resource
    Electronic Resource
    Identification of macrophages and smooth muscle cells with monoclonal antibodies in the human atherosclerotic plaque (1987)
    Springer
    Virchows Archiv 412 (1987), S. 169-174 
    Details
    ISSN: 1432-2307
    Keywords: Human atherosclerotic plaque ; Phenotypic characterization of cell types ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Sections of human atherosclerotic plaques, obtained from 21 autopsy cases with various degrees of atherosclerosis, were stained with the indirect immunoperoxidase technique using specific monoclonal antibodies against macrophages and smooth muscle cells. Distinctive results were found in differing stages: Single blood monocytes were observed in diffuse intimal thickening and the foam cells seen in fatty streaks were mostly identified as mature tissue macrophages, while only very few blood monocytes were present. The spindle cells observed in fibroelastic plaques showed positive reactions to antibodies against desmin, which points to their derivation from smooth muscle cells, whereas only a few macrophage-derived foam cells were seen in these lesions. In the complicated lesions the majority of foam cells were macrophage-derived, but there was also a small number of foam cells positive to antibodies against desmin, suggesting a smooth muscle cell derivation. - Our results confirm that in human atherosclerotic plaques the majority of the foam cells are obviously macrophage-derived, which emphasizes the important role of macrophages in the morphogenesis of these lesions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00716190
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