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  • 1
    Electronic Resource
    Electronic Resource
    A three-step procedure for the purification of human basophils from buffy coat blood (1997)
    Springer
    Inflammation research 46 (1997), S. 137-142 
    Details
    ISSN: 1420-908X
    Keywords: Key words: Basophil — Purification — Elutriation — Immunomagnetic bead — Histamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Objective and Design: We report a method for basophil purification from buffy coats, which avoids positive selection of the cells and gives rise to good purity, yield and functional integrity of the cells.¶Subjects: Buffy coat blood (concentrated leukocyte fraction derived from 450 ml venipuncture donations) obtained from healthy blood donors (n = 51).¶Methods: Basophils were enriched by a three-step process starting with Ficoll density centrifugation (1.6 ± 0.1% basophil purity) followed by counter current centrifugal elutriation (17.7 ± 1.4% basophil purity). The final stage involved negative selection using Dynal immunomagnetic beads directed against CD2, CD14, CD16 and CD19 positive cell contaminants. Functional integrity of which was assessed by comparing the anti-IgE or calcium ionophore A23187 induced histamine release from basophils obtained from each enrichment step. Furthermore, basophil morphology was investigated using light and electron microscopy.¶Results: The final mean basophil purity of 67.3 ± 1.4% with a yield of 3.5 ± 0.5 × 106 basophils and a recovery of 21.8 ± 2.4% was achieved. Net histamine release from basophils stimulated with optimal concentrations of anti-human IgE was 39.1 ± 6.5% after Ficoll centrifugation, 41.6 ± 7.7% following elutriation and 35.7 ± 6.8% from the final purified fraction. Additionally, basophils enriched with our method showed intact morphology by electron microscopy and were functionally active to non-immunological stimulation.¶Conclusions: These results compare favourably with previous studies, which have often required the use of positive selection via the Fc〈epsilon〉RI receptor, which may result in cell degranulation, or cell sorting, which cannot be applied to large cell numbers. Our method provides a reproducible technique for basophil enrichment when large numbers of functionally intact basophils are required.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s000110050537
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  • 2
    Electronic Resource
    Electronic Resource
    Über eine Impfnekrose (1921)
    Springer
    Archives of dermatological research 136 (1921), S. 328-332 
    Details
    ISSN: 1432-069X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01843140
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  • 3
    Electronic Resource
    Electronic Resource
    Increased Permeability of the Gut to Polyethylene Glycol and Dextran in Rats Fed Alcohol (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 625 (1991), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1991.tb33931.x
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  • 4
    Electronic Resource
    Electronic Resource
    Immunohistological Double Labeling of Apolipoprotein E in Different Cell Types of the Human Atherosclerotic Plaque (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 598 (1990), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1990.tb42331.x
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  • 5
    Electronic Resource
    Electronic Resource
    Identification of macrophages and smooth muscle cells with monoclonal antibodies in the human atherosclerotic plaque (1987)
    Springer
    Virchows Archiv 412 (1987), S. 169-174 
    Details
    ISSN: 1432-2307
    Keywords: Human atherosclerotic plaque ; Phenotypic characterization of cell types ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Sections of human atherosclerotic plaques, obtained from 21 autopsy cases with various degrees of atherosclerosis, were stained with the indirect immunoperoxidase technique using specific monoclonal antibodies against macrophages and smooth muscle cells. Distinctive results were found in differing stages: Single blood monocytes were observed in diffuse intimal thickening and the foam cells seen in fatty streaks were mostly identified as mature tissue macrophages, while only very few blood monocytes were present. The spindle cells observed in fibroelastic plaques showed positive reactions to antibodies against desmin, which points to their derivation from smooth muscle cells, whereas only a few macrophage-derived foam cells were seen in these lesions. In the complicated lesions the majority of foam cells were macrophage-derived, but there was also a small number of foam cells positive to antibodies against desmin, suggesting a smooth muscle cell derivation. - Our results confirm that in human atherosclerotic plaques the majority of the foam cells are obviously macrophage-derived, which emphasizes the important role of macrophages in the morphogenesis of these lesions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00716190
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  • 6
    Electronic Resource
    Electronic Resource
    Macrophages and T lymphocytes infiltrating the rat mammary carcinoma HH9-cl 14 in progressive and regressive tumor growth (1986)
    Springer
    Journal of cancer research and clinical oncology 111 (1986), S. 13-19 
    Details
    ISSN: 1432-1335
    Keywords: Tumor model ; Immune surveillance ; Macrophages ; T Lymphocytes ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Ascites tumor cells (2×106) of a DMBA-induced rat mammary adenocarcinoma (HH9-cl 14) were injected s.c. into tumor-free syngeneic female rats and produced a continuously growing solid tumor in all animals of this group. Inoculation of 2×107 cells induced a first brief period of tumor growth, followed by complete tumor regression from the 2nd until the 5th week after injection. Both the progressive and the regressive tumors were analyzed immunohistologically at different stages with monoclonal antibodies against different T lymphocytes and macrophages. Obviously these cells appear in different quantity and quality, during the hosts immune response. Possible interactions of T lymphocytes and macrophages with tumor cells are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402769
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  • 7
    Electronic Resource
    Electronic Resource
    Changes in intestinal mucosa above lymph follicles during carcinogenesis in rats (1987)
    Springer
    Journal of cancer research and clinical oncology 113 (1987), S. 41-50 
    Details
    ISSN: 1432-1335
    Keywords: Experimental carcinogenesis ; Lymphoid plaque of rat intestine ; 1,2-Dimethylhydrazine-2HCl (DMH) ; Gut endocrine cell ; Rat intestinal carcinoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary p Changes in the intestinal mucosa during carcinogenesis were investigated in 36 rats after weekly s.c. injection of 20 mg dimethylhydrazine/kg body-weight. More changes were seen in the large than in the small intestine. In the first week, 60% of colonic lymphoid plaques displayed various crypt abscesses and glandular regenerations. These mucosal changes correspond to the glands covering the lymph follicles, in direct contact with lymphoid cells. Beginning in week 8, dysplastic glands developed in these mucosal areas above the lymph follicles. The number of lymphoid plaques with dysplastic glands in the large intestine increased week by week, attaining 75% in week 20. At the end of week 12 the first adenocarcinoma was detected in the cecum by light microscopy, and classified as a poorly differentiated adenocarcinoma with signet ring cells infiltrating the lymph follicles which contained endocrine cells. The majority of adenocarcinomas (10 cases) occurred in week 20. of these, 7 were localized above the lymphatic plaques in the intestine. Endocrine cells were found in varying numbers in 6 of 10 adenocarcinomas. Three endocrine cell carcinomas, corresponding to human adenocarcinoids or goblet cell carcinoids, developed within the intestinal mucosa; all were identified as poorly differentiated intestinal adenocarcinomas, two of them situated above lymph follicles. These suprafollicular tumors developing from the glandular base, were composed of mucoid cells, endocrine cells, and undifferentiated cells. Microcarcinomas are considered as initial stages of endocrine cell carcinoma. The trend of tumor development above colonic lymph follicles, and the histogenesis of endocrine cell carcinomas and de novo carcinomas is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00389965
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  • 8
    Electronic Resource
    Electronic Resource
    Biological characterization of human bone tumors (1987)
    Springer
    Journal of cancer research and clinical oncology 113 (1987), S. 559-562 
    Details
    ISSN: 1432-1335
    Keywords: Immunohistological techniques ; Monoclonal antibodies ; Mononuclear phagocyte system ; Malignant fibrous histiocytoma ; Giant cell tumor of bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Seven giant cell tumors of bone and four malignant fibrous histiocytomas were studied immunohistochemically with different monoclonal antibodies to the mononuclear phagocyte system (MPS), to HLA-DR antigens, and to a proliferation-associated nuclear antigen (KI-67), in order to clarify the role of macrophages in these tumors. A part of the mononuclear cells stained positive with antibodies against the MPS. Antibody 25-F-9 against mature tissue macrophages showed the strongest reaction. The osteoclast-like giant cells also stained positive with this antibody. Fibroblast-like stromal cells, however, showed negative reactions to all antibodies against MPS cells. A double-labeling immunohistological technique was used to detect the proliferating cell population in these tumors. The fibroblast-like cells that were negative for MPS markers, were positively labeled with the monoclonal antibody Ki-67 against a proliferation-associated nuclear antigen, whereas a negative reaction to Ki-67 was seen in cells positive with antibodies to the MPS. These results support the concept that macrophages are a reactive population in these tumors, whereas the fibroblast-like mesenchymal cells are the proliferating tumor cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390865
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  • 9
    Electronic Resource
    Electronic Resource
    Licht- und Elektronenmikroskopie der Steroid-Rezeptoren in Tube und Uterus (1993)
    Springer
    Archives of gynecology and obstetrics 254 (1993), S. 188-189 
    Details
    ISSN: 1432-0711
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Schlußfolgerung Unsere Untersuchungen zeigen somit sowohl auf licht- als auch auf elektronenmikroskopischer Ebene, daß die gefundenen Varationen in der Expression der Steroidrezeptoren als Ausdruch einer zum Teil reversiblen Zellschädigung zu verstehen sind, die mit einer Funktionsbeeinträchtigung während des Eitransportes einhergehen. Unsere Ergebnisse weisen auf eine Mitbeteiligung der Steroide und ihrer Rezeptoren bei der komplexen und im einzelnen noch wenig verstandenen Regulation des Eitransportes hin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02265951
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  • 10
    Electronic Resource
    Electronic Resource
    Steriod-Rezeptoren und Mamma-Karzinom (1993)
    Springer
    Archives of gynecology and obstetrics 254 (1993), S. 801-802 
    Details
    ISSN: 1432-0711
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Schlußfolgerung Somit erweist sich die immunohistochemische Östrogen-Rezeptor-und Progesteron-Rezeptor-Bestimmung gegenüber dem biochemischen Steroid-Rezeptor-Nachweis als empfindliche Methode. Zudem zeigt sich keine Korrelation zwischen der Höhe des Progesteron-Rezeptors und der Zykluphase z.Z. der Brustoperation. Wie Badwe und Mitarbeiter (2) beim Vergleich Überlebenszeit in Korrelation zur Zyklusphase z.Z. der Brustoperation beim Mammakarzinom gezeigt haben, müssen auch nach unseren vorliegenden Ergebnissen der immunohistochemischen Steroid-Rezeptorbestimmung weitere Faktoren als das Vorhandensein des Progesteron-Rezeptors für das bessere Überleben der in der Luteal-Phase operierten Patientin wesentlich sein, wie z.B. autokrine oder parakrine Interaktionen über Wachstumsfaktoren oder Zytokine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02266181
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