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Immunohistochemical identification of corticotropin releasing factor-containing neurons projecting to the stalk-median eminence of the rat

Niimi, M. ; Takahara, J. ; Hashimoto, K. ; [et al.]

Amsterdam : Elsevier

Peptides 9 (1988), S. 589-593

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ISSN: 0196-9781

Keywords: Corticotropin releasing factor (CRF) ; Immunocytochemistry ; Median eminence ; True blue

Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Topics: Chemistry and Pharmacology

Type of Medium: Electronic Resource

URL: http://linkinghub.elsevier.com/retrieve/pii/0196-9781(88)90169-6

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Sites of origin of growth hormone-releasing factor-containing neurons projecting to the stalk-median eminence of the rat

Niimi, M. ; Takahara, J. ; Sato, M. ; [et al.]

Amsterdam : Elsevier

Peptides 10 (1989), S. 605-608

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ISSN: 0196-9781

Keywords: Growth hormone-releasing factor (GRF) ; Immunocytochemistry ; Median eminence ; True blue

Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Topics: Chemistry and Pharmacology

Type of Medium: Electronic Resource

URL: http://linkinghub.elsevier.com/retrieve/pii/0196-9781(89)90150-2

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Calphostin C synergistically induces apoptosis with VP-16 in lymphoma cells which express abundant phosphorylated Bcl-2 protein (1997)

Murata, M. ; Nagai, M. ; Fujita, M. ; [et al.]

Springer

Cellular and molecular life sciences 53 (1997), S. 737-743

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ISSN: 1420-9071

Keywords: Key words. Apoptosis; Bcl-2; protein kinase C; follicular lymphoma; phosphorylation.

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract. A newly established human lymphoma cell line (OZ) has the t(14;18)(q32;q21) translocation and expresses large amounts of Bcl-2 compared to CCRF-CEM cells. VP-16 (40 μg/mL), a promising agent against lymphoma, caused DNA fragmentation (26.9% of total DNA) typical for apoptosis at 6 h in CCRF-CEM cells, but no significant changes in OZ cells until 24 h after the addition of VP-16. However, coincubation with calphostin C (0.2 μg/mL), a protein kinase C (PKC) inhibitor, induced DNA fragmentation in VP-16-treated OZ cells (13.5% of total DNA) at 6 h after the treatment. Simultaneous immunoblot analysis revealed that this induction of apoptosis coincided with the downregulation of serine-phosphorylated Bcl-2 (13% of control cells). By contrast, apoptosis induced by VP-16 in CCRF-CEM cells was attenuated by the addition of 0.5 μM phorbol 12-myristate 13-acetate, a potent PKC stimulator. These observations suggest that Bcl-2 function is partly regulated by phosphorylation/dephosphorylation mechanisms of the PKC system, and that phosphorylated Bcl-2 in lymphoma cells may play a role in the prevention of apoptosis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s000180050093

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Elevation of Anti-Cytokeratin 18 Antibody and Circulating Cytokeratin 18: Anti-Cytokeratin 18 Antibody Immune Complexes in Sera of Patients with Idiopathic Pulmonary Fibrosis (2000)

Dobashi, N. ; Fujita, J. ; Murota, M. ; [et al.]

Springer

Lung 178 (2000), S. 171-179

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ISSN: 1432-1750

Keywords: Key words: Autoantibody—Cytokeratin 18—Idiopathic pulmonary fibrosis—Immune complexes

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract. In this study, we hypothesize that anti-cytokeratin 18 (CK18) antibody and CK18:anti-CK18 immune complex increase in sera in patients with idiopathic pulmonary fibrosis (IPF). To prove the existence of anti-CK18 antibodies in patients' sera, bovine CK18 was stained with patients' sera using a Western blotting. In patients with IPF, anti-CK18 antibodies were clearly demonstrated in sera by Western blotting. Then, we tried to establish an enzyme-linked immunosorbent assay (ELISA) to quantify anti-CK18 antibodies and CK18:anti-CK18 immune complexes in sera of patients with IPF. Levels of anti-human CK18 antibodies in sera of patients with IPF (0.81 ± 0.31, mean ± SD) measured by ELISA were significantly high compared with that of normal volunteers (0.45 ± 0.06, p 〈 0.01). In addition, levels of CK18:anti-CK18 antibody complexes in patients' sera (0.64 ± 0.35, man ± SD) significantly increased compared with those of normal subjects (0.40 ± 0.10, p 〈 0.01). These results suggest that anti-CK18 antibody and its immune complex may have played a role in the process of lung injury in IPF.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004080000020

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Differences in the distributional pattern of CRF-, oxytocin-, and vasopressin-immunoreactive nerve fibers in the median eminence of the rat (1983)

Kawata, M. ; Hashimoto, K. ; Takahara, J. ; [et al.]

Springer

Cell & tissue research 230 (1983), S. 247-258

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ISSN: 1432-0878

Keywords: CRF ; Oxytocin ; Vasopressin ; Immunohistochemistry ; Median eminence ; Rat

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary Conspicuous differences in the distributional pattern of nerve fibers containing corticotropin-releasing factor (CRF) or posterior lobe hormones, respectively, were shown in the median eminence of the adult male rat by means of immunoperoxidase histochemistry, with the use of anti-CRF, anti-oxytocin, and anti-vasopressin sera. In the rostral and central divisions of the median eminence, a high concentration of CRF-immunoreactive nerve fibers was found in the median portion of the external layer; these fibers terminated on the capillary loops of the hypophysial portal system. In the caudal division of the median eminence, the CRF-immunoreactive nerve fibers were located in the median to paramedian portions of the external layer. Numerous oxytocin- and vasopressin-immunoreactive nerve fibers were observed evenly distributed throughout the internal layer of the median eminence. In the external layer, a small number of the oxytocin- and vasopressin-containing nerve fibers was found around the capillary loops, particularly in the median to paramedian portions. The distributional patterns of the CRF and the posterior lobe hormones in the hypothalamo-hypophysial system and their functional interrelationship are discussed.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00213803

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Immunohistochemical identification of neurons containing corticotropin-releasing factor in the rat hypothalamus (1983)

Kawata, M. ; Hashimoto, K. ; Takahara, J. ; [et al.]

Springer

Cell & tissue research 230 (1983), S. 239-246

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ISSN: 1432-0878

Keywords: CRF neurons ; Hypothalamus ; Immunohistochemistry ; PAP ; Rat

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Summary A specific rabbit anti-CRF serum and the immunoperoxidase technique were used to show that CRF-containing neurons are mainly distributed in the paraventricular and supraoptic nuclei of the rat hypothalamus. In addition, immunoreactive neurons are scattered in other hypothalamic regions. These neurons are 20–30 μm in diameter. From the present and previous investigations it may be concluded that the hypothalamic magnocellular nuclei, i.e., paraventricular and supraoptic, and other hypothalamic accessory nuclei, are the producing sites not only for vasopressin and oxytocin, but also for corticotropin-releasing factor.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00213802

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