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  • 11
    Electronic Resource
    Electronic Resource
    Über das postnatale Wachstum des Corpus callosum der Katze (Felis domestica) (1970)
    Springer
    Anatomy and embryology 132 (1970), S. 228-239 
    Details
    ISSN: 1432-0568
    Keywords: Corpus callosum ; Postnatal growth ; Cat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Das postnatale Wachstum des Corpus callosum der Katze wurde an 31 Tieren im Alter von 1 Tag bis zu 15 Jahren untersucht. Die Gehirne wurden durch Perfusion mit Bouinscher Lösung fixiert und in 21 Frontal- und 10 Sagittalserien zerlegt. Folgende Befunde wurden erhoben: 1. Die Breite des Corpus callosum — und damit die Länge der darin verlaufenden Axone — nimmt während der postnatalen Entwicklung von etwa 2,8 mm auf über 5 mm zu. Das Wachstum erfolgt in den ersten 50 Tagen und ist vor der Geschlechtsreife abgeschlossen. 2. Die an Frontalschnitten ermittelte Fläche nimmt von etwa 1,4 mm2 auf über 3 mm2 zu. Das Wachstum setzt um den 15. Tag ein und ist mit Erreichen der Geschlechtsreife noch nicht abgeschlossen. Die an Sagittalschnitten ermittelte Fläche zeigt einen ähnlichen Wachstumsverlauf und nimmt von etwa 6 mm2 auf über 11 mm2 zu. 3. Das aus Fläche und Breite errechenbare Volumen des Corpus callosum nimmt nach dem 15. Lebenstag zu. Das Wachstum hält während der ganzen postnatalen Entwicklungsphase an. Das Volumen des Corpus callosum erreicht bei einem Alter von 1 1/2 Jahren mit über 60 mm3 etwa das 3fache des Volumens bei der neugeborenen Katze. Bei Berücksichtigung der durch Fixierung und Paraffineinbettung bedingten Schrumpfung hat der Balken der erwachsenen Katze demnach ein tatsächliches Volumen von etwa 85 mm3.
    Notes: Summary The postnatal growth of the corpus callosum has been studied in 31 cats of various ages between 1 day and 15 years. The brains were fixed by perfusion with Bouin's fluid and cut in the frontal (21) and sagittal (10) planes. The following results were obtained: 1. The width of the corpus callosum which is a measure for the length of the callosal nerve fibres increases from 2.8 mm to about 5 mm. This increase takes place during the first 50 days of postnatal life and comes to an end before sexual maturity is reached. 2. The square area of the corpus callosum as measured in frontal sections increases from 1.4 mm2 to more than 3 mm2. The growth begins at about the 15th day and continues into adult life. The square area as measured in sagittal sections shows a similar pattern of growth and increases from 6 mm2 to about 11 mm2. 3. The volume of the corpus callosum as determined from the values for width obtained in frontal sections and from the values for square area as determined in sagittal sections, increases from about the 15th day after birth and continues to increase after sexual maturity is reached. At 1 1/2 years of age the volume of the corpus callosum is about 60 mm3, i.e. three times that of the newborn cat. When the shrinkage due to fixation and embedding in paraffin is taken into account, the volume of the corpus callosum in the adult cat reaches a value of about 85 mm3.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00523378
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  • 12
    Electronic Resource
    Electronic Resource
    Ultrastructural changes of the early visceral yolk sac layer of mouse embryos after maternal injection of trypan blue (1978)
    Springer
    Anatomy and embryology 153 (1978), S. 287-293 
    Details
    ISSN: 1432-0568
    Keywords: Mouse embryos ; Visceral yolk sac ; Trypan blue ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The ultrastructural features of the early visceral yolk sac epithelium of normal mouse embryos on day 9 were compared to those whose mothers had received a single subcutaneous injection of 100 mg/kg trypan blue on day 8. The following results were obtained: In normal embryos the visceral yolk sac cells are predominantly characterized by numerous membrane bounded inclusions localized in the supranuclear cytoplasm. In embryos of animals treated with trypan blue, at about 12h after injection large single and only partly membrane bounded vacuoles are observed occupying most of the apical cytoplasm. Up to 24h after injection large cytoplasmic areas are seen which are in a stage of autodigestion possibly due to leakage of the vacuolar content. These alterations are exclusively limited to the visceral yolk sac epithelium whereas in the cells of the embryonic part, e.g. head process, no changes could be found. The observations are discussed in relation to the mechanism of the teratogenic activity of trypan blue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00315931
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  • 13
    Electronic Resource
    Electronic Resource
    Über die embryotoxische Wirkung von Carmin bei der Maus (1970)
    Springer
    Anatomy and embryology 131 (1970), S. 228-235 
    Details
    ISSN: 1432-0568
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die embryotoxische Wirkung von Carmin wurde an 2 Gruppen trächtiger Mäuse (NMRI/Han) nach subcutaner Injektion von Lithiumcarmin und Sodacarmin am 8. Tag der Gravidität geprüft. 10 Kontrolltiere erhielten 0,5 ml physiol. NaCl. Alle Tiere wurden am 19. Trächtigkeitstag schnittentbunden. Die Zahl der resorbierten, mißgebildeten und retardierten Feten wurde ermittelt und folgende Befunde erhoben: 1. Die Zahl der Resorptionen beträgt gegenüber 2% bei den Kontrollen nach Gabe von Lithiumcarmin oder Sodacarmin annähernd 20%. 2. Die Mißbildungsrate liegt nach Injektion von Lithiumcarmin um 16%, nach Injektion von Sodacarmin um 2,5%. Die häufigsten Mißbildungsformen sind Exencephalien und Rippen-Wirbelsäulen-Mißbildungen. 3. Die Zahl der retardierten Feten liegt nach Injektion von Lithiumcarmin mit etwa 4% unter der Kontrollgruppe (6,7%); bei Injektion von Sodacarmin ist sie mit 22% gegenüber den Kontrolltieren erhöht. Die Retardierungen sind am häufigsten im Bereich des Brustbeins und der Schädeldeckknochen lokalisiert. 4. Carmin wird ebenso wie Trypanblau im visceralen Dottersackepithel gespeichert. Die Bedeutung des Dottersackes beim Zustandekommen der embryotoxischen Carminwirkung wird diskutiert.
    Notes: Summary The embryotoxic action of carmine in pregnant mice has been investigated by means of subcutaneous injections of lithium carmine and sodium carmine at day 8 of gestation. The control animals were injected with 0.5 ml of saline. All animals were killed at day 19 and the number of resorbed, malformed and retarded fetuses was examined. The findings are as follows: 1. Following the injection of lithium carmine and sodium carmine, the number of resorptions rises to about 20% compared with about 2% in the controls. 2. Following the application of lithium carmine, the malformation rate is about 16% and following the injection of sodium carmine about 2.5%. Most commonly found are malformations of the ribs and of the vertebral column as well as exencephalies. 3. Following administration of lithium carmine the number of retarded fetuses is found to be about 4%. This is less than in the controls (6.7%). After injection of sodium carmine, the retardation rate is increased to approximately 22%. The most frequent retardations are localized in the sternum and the calvaria. 4. Carmine, similar to trypan blue, is concentrated in the visceral layer of yolk sac epithelium. The importance of the yolk sac as a nutritive organ of the developing embryo is discussed in the relation to the embryotoxic action of carmine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00520965
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  • 14
    Electronic Resource
    Electronic Resource
    DNA-, histone-, RNA-, hemoglobin-content and DNA-synthesis in erythroblasts in a case of congenital dyserythropoietic anemia type I (1972)
    Springer
    Annals of hematology 24 (1972), S. 32-41 
    Details
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Erythroblasten eines Falles von dyserythropoetischer Anämie Typ I wurden mit Hilfe zytophotometrischer Techniken untersucht. Diese wurden kombiniert mit der autoradiographischen Analyse des3H-Thymidin-Einbaus in vitro. Bei einem großen Teil der Erythroblasten wurden hypertetraploide DNS-Werte beobachtet — das Substrat einer gestörten Koordination von DNS-Synthese und Zellzyklus. Die Fähigkeit zur DNS-Synthese ging häufig schon bei unreifen Eruthroblasten irreversibel verloren, was zu einer intramedullären Akkumulation von nicht proliferierenden und allmählich zerfallenden Zellen führte. Der Quotient der fast-green-Feulgen-Extinktion war erhöht. Dieser Befund wies auf eine Störung des Desoxyribonukleoprotein-Stoffwechsels bei unreifen Erythroblasten hin. Die RNS-Synthese war stark reduziert, was eine Störung der Hb-Synthese zur Folge hatte.
    Notes: Summary Erythroblasts of a case of congenital dyserythropoietic anemia type I were examined using cytophotometric techniques together with an analysis of3H-thymidine incorporation in vitro by autoradiography. Hypertetraploid DNA-values were observed in a high proportion of the erythroblasts, obviously resulting from a disturbed coordination of DNA-synthesis and the cell cycle. There was evidence that DNA-synthesis was irreversibly arrested in a high proportion of early erythroblasts giving rise to an intramedullar accumulation of non-proliferating gradually decomposing cells. The fast green-Feulgen extinction ratio was increased suggesting the presence of an altered deoxyribonucleoprotein metabolism in immature erythroblasts. RNA-synthesis was markedly reduced causing a disturbance of Hb-synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01633140
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  • 15
    Electronic Resource
    Electronic Resource
    Proportionalitätsfehler bei der Feulgen-Hydrolyse (1968)
    Springer
    Histochemistry and cell biology 15 (1968), S. 194-203 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Beim Vergleich des Feulgen-Farbgehaltes verschiedener Zellkerne (Leberzellen, Lymphozyten, Granulozyten und Spermien) traten nach Alkoholfixierung Abweichungen der gemessenen Feulgen-Werte von den nach dem Gesetz von der DNS- Konstanz zu erwartenden DNS-Gehalt dieser Kerne auf. Verglichen mit den Feulgen-Werten der diploiden Leberzellkerne ergaben Lympho- und Granulozyten bei allen Hydrolysezeiten zu niedrige (bis zu minus 20%), haploide Spermien im postmaximalen Hydrolysebereich zu hohe Feulgen-Werte (z. T. sogar höhere Werte als die diploiden Zellkerne). Innerhalb desselben Zelltypes wurden dagegen, auch beim Vergleich der verschiedenen Ploidiestufen der Leberkerne, keine Differenzen festgestellt. Da die an Leukozyten und Spermien beobachteten Abweichungen nach Methanol-Formalin-Eisessig-Fixierung nicht mehr auftraten und auch durch UV-absorptionscytophotometrische Messungen nicht bestätigt werden konnten, muß man annehmen, daß es sich um Proportionalitätsfehler handelt, die auf Hydrolyseunterschieden beruhen. Für die quantitative Feulgen-Cytophotometrie scheint daher die Methanol-Formalin-Eisessig-Fixierung besser geeignet zu sein als die Alkoholfixierung, bei deren Verwendung es leicht zu Proportionalitätsfehlern während der Feulgen-Hydrolyse kommen kann.
    Notes: Summary Comparing the Feulgen dye-content of different nuclei (liver cells, lymphocytes, granulocytes and sperms) after alcohol-fixation deviations were found between the measured Feulgen values and the DNA-content to be expected from the DNA-constancy law. The Feulgen values of lymphocytes and granulocytes proved to be lower (up to minus 20%) than those of diploid liver nuclei at all hydrolysis times, while in the postmaximal range of hydrolysis the values of the haploid sperms were too high (even higher than those of the diploid nuclei). Such differences did not appear when nuclei of the same cell type in different DNA- ploidy classes (liver nuclei) were compared. Those deviations of leucocytes and sperms were no longer found after fixation in methanol-formalin-glacial acetic acid and, in addition, could not be confirmed by UV-absorption measurements. For that reason we suppose them to be due to proportionality errors caused by variations in the hydrolytic behaviour of the different nucleoproteins. Thus fixation in methanol-formalin-glacial acetic acid seems to be more suitable for quantitative Feulgen measurements than alcohol-fixation, which may easily give rise to proportionality errors during Feulgen hydrolysis. Moreover, to avoid any false interpretation of Feulgen data we should suggest controll measurements using another independent method (f. e. UV-absorption), or — if this is impossible — to check the Feulgen values after different fixations and variant hydrolysis times (premaximal, maximal, postmaximal).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00305883
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  • 16
    Electronic Resource
    Electronic Resource
    Fluoreszenzmikroskopischer Nachweis von Arginin im Neurosekret von Säugern (1971)
    Springer
    Histochemistry and cell biology 25 (1971), S. 152-162 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Bei Hunden, Hausschweinen, Wistarratten und Albinomäusen wurde das Vorkommen von Arginin im Neurosekret des supraoptico-hypophysären Systems mit der von Rosselet angegebenen Fluoreszenzreaktion untersucht. Das Verfahren basiert auf der Bildung einer fluoreszierenden Verbindung durch Anlagerung eines im alkalischen Milieu entstehenden Umwandlungsproduktes des Ninhydrins an die Guanidogruppe des Arginins. Bei allen aufgeführten Spezies zeigte das neurosekretorische Material im Hypophysenhinterlappen eine positive Argininreaktion. Auch in den Zellen der Nuclei supraopticus und paraventricularis konnte bei allen Spezies mit Ausnahme der Maus Arginin nachgewiesen werden. Dagegen ließen sich mit der Methode bei keiner Spezies die Fasern des Tractus supraoptico-hypophyseus hervorheben. Die Argininspezifität der Reaktion wurde durch mikrospektrographische Untersuchungen bestätigt. Aus dem Nachweis von Arginin im Neurosekret des Schweines folgt, daß es sich bei dem neurosekretorischen Material nicht um die Hinterlappenhormone selbst, sondern nur um deren Trägerproteine handeln kann.
    Notes: Summary In the neurosecretory material of the supraoptico-hypophysial system of dogs, pigs, rats, and mice arginine was shown to occur by means of the method of Rosselet. This method is based on the fact that arginine reacts with a ninhydrin derivate to give a fluorescent compound which can be localized in histological sections. In all species investigated, the neurosecretory material in the pituitary posterior lobe has been found to give a positive reaction. In addition, arginine was found in the nerve cells of the supraoptic and paraventricular nuclei of all species except mice. In contrast, no arginine could be demonstrated in the tractus supraoptico-hypophyseus of any of the species investigated. The arginine specificity of the reaction was confirmed by microspectrographic investigations. From the finding that the neurosecretory material of pigs contains arginine it can be concluded, that this material is not identical with the posterior lobe hormones, because in the pig these hormones are free of arginine. It is thus likely that the neurosecretory material represents the carrier protein of the hormones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279113
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  • 17
    Electronic Resource
    Electronic Resource
    Untersuchungen Zur Eosinophilie Hyalinen Knorpels (1966)
    Springer
    Histochemistry and cell biology 7 (1966), S. 303-317 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Während der chemischen Fixierung von Geweben und der nachfolgenden Behandlung in Alkoholreihen geht Hämoglobin in Lösung und diffundiert in das Gewebe. Die Diffusion des Hämoglobins ist besonders gut in der Grundsubstanz hyaliner Knorpel zu verfolgen. Das eingedrungene Hämoglobin wurde mit der Ultramikrospektrographie nachgewiesen. Die Hämoglobindiffusion läßt sich durch die Kryostatschnittechnik und durch eine Fixierung in 4%igem Formalin mit Zusatz von 2,5% Ferricyankalium verhindern bzw. mit Zusatz von Natriumchlorid oder Phosphat zu Formalin einschränken. Besonders säurehaltige Fixierungslösungen trennen die Häm- von der Globinkomponente. Der Globinanteil verbleibt in der Grundsubstanz des Knorpels und kann das Vorliegen eines ortsständigen Eiweiß-körpers vortäuschen. Die Einlagerung des vollständigen Hämoglobins oder des Globins allein bedingen die diffuse Eosinophilie der Grundsubstanz im fixierten Knorpel und blockiert die Darstellung der sauren Mucopolysaccharide (Alcianblaufärbung) sowie die metachromatische Reaktion der Grundsubstanz.
    Notes: Summary In the course of chemical fixation of tissues and the subsequent treatment with alcohol hemoglobin dissolves and diffuses into the tissue. The diffusion of hemoglobin can be observed particularly well in the ground substance of hyaline cartilage. There the hemoglobin is demonstrable by means of ultramicrospectrography. The diffusion of the hemoglobin can be prevented through the use of the cryostat technique and fixation in 4% formalin containing 2.5% ferricyankalium, NaCl or phosphate. Acid containing fixation media separate the “hem” from the “globin” component. The globin remains in the ground substance of the cartilage and may be mistaken for a sessil protein. The storage of either the complete hemoglobin molecule or the globin fraction causes the diffuse eosinophily in the ground substance of fixed cartilage which also blocks the determination of acid mucopolysaccharides (alcian blue staining method) as well as the metachromatic reaction of the ground substance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00306617
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  • 18
    Electronic Resource
    Electronic Resource
    Eine cytophotometrische Methode zur Objektivierung der Morphologie von Zellkernen (1967)
    Springer
    Histochemistry and cell biology 10 (1967), S. 341-352 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Es wird eine cytophotometrische Methode zur Objektivierung des morphologischen Erscheinungsbildes von feulgengefärbten Zellkernen angegeben. Nach photometrischer Abtastung der Zellkerne werden die Chromatinpartikel nach ihrem Farbstoffgehalt in verschiedene Extinktionsstufen eingeordnet. Diese Klassifizierung des Kernchromatins ergibt für verschiedene Typen von Zellkernen unterschiedliche Häufigkeitsverteilungen in verschiedenen Extinktionsstufen. Es können drei Klassen von Zellkernen unterschieden werden. 1. Kleine dichte Zellkerne, bei denen 70% der Werte in der höchsten Extinktionsstufe liegen und 30% niedrigeren Extinktionsstufen angehören (Thymuslymphozyten). 2. Große Zellkerne (Leberzellen, Mäuseascitestumorzellen), bei denen 90% der Extinktionswerte einer einheitlichen mittleren Extinktionsstufe angehören (unimodale Verteilung der Partikelextinktion). 3. Mittelgroße Zellkerne (Thymuslymphoblasten, Kupffersche Sternzellen), bei denen zwei Populationen von Chromatinpartikeln, solche mit niedrigen und solche mit hohen Extinktionswerten auftreten. Es wird diskutiert, inwieweit diese Methode für die Mengenbestimmung von Eu- und Heterochromatin benutzt werden kann.
    Notes: Summary A method for the objectivation of the morphology of Feulgen stained cell nuclei is presented. Cytophotometric measurements (scanning method) were used to obtain the optical density of chromatin particles. The particles were classified according to their optical density (Extinction level separation method). This classification of the nuclear chromatin particles reveals characteristic frequency distribution in different types of cell nuclei. Three classes of the cell nuclei may be distinguished. 1. Small dense cell nuclei in which 70% of the extinction values fall into the highest extinction level class while 30% belong to lower extinction levels (Thymus lymphocytes). 2. Large cell nuclei (liver cells, mouse ascites tumor cells) in which 90% of the optical density values of the particles are arranged around a medium value showing the presence of an unimodal frequency distribution pattern. 3. Medium-large cell nuclei (Thymus lymphoblasts, Kupffer cells), in which 2 populations of chromatin particles are found; one with a low and one with high extinction values. The possibility to use this method for quantitative estimation of Eu- and Heterochromatin is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00304317
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  • 19
    Electronic Resource
    Electronic Resource
    Urinary antigens as markers of papillary toxicity. I Identification and characterization of rat kidney papillary antigens with monoclonal antibodies (1996)
    Springer
    Archives of toxicology 71 (1996), S. 80-92 
    Details
    ISSN: 1432-0738
    Keywords: Key words Rat papillary antigens ; Monoclonal antibodies ; Nephrotoxicity ; Papillary toxicity ; Urinary markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Monoclonal antibodies were prepared in an attempt to develop diagnostic tools for the identification of toxic damage to the rat renal papilla. One IgG and five IgM monoclonal antibodies, reacting with antigens localized in the papilla were obtained. Three of the IgM class and the IgG class monoclonal antibodies were found to be specific for antigens localized in collecting ducts, two of them staining papillary collecting ducts more intensely than cortical collecting ducts. The IgG class antibody, termed Pap X 5C10, recognizes an antigen located at high density on the luminal side of papillary collecting duct epithelial cells and at lower density in cortical collecting duct cells. One of the IgM class monoclonal antibodies reacts with an antigen localized in epithelial cells of ascending and descending loops of Henle and of connecting tubules. Another of the IgM class monoclonal antibodies reacts with an antigen localized in the interstices of the inner medulla. All these monoclonal antibodies react with their antigens in native frozen as well as in Bouin-fixed and paraffin-embedded tissue slices. Molecular properties of the Pap X 5C10 antigen have been investigated by gel permeation chromatography, SDS-PAGE, Western blotting, and isoelectric focusing. The results indicate that the antigen in both its tissue-derived and urinary form is of large (150–200 kDa) molecular size and can be separated into two molecular species with isoelectric points of pH 7.2 and 7.3 respectively. In the urine the antigens recognized by the monoclonal antibodies form large complexes with Tamm-Horsfall protein. The antigen-containing complexes can be extracted from urine by adsorption to diatomaceous earth and elution with SDS-containing buffer. Using sandwich ELISA-type assays it is possible to determine the concentration of the antigens. In preliminary experiments we were able to show that at least three of the antigens are detected in the urine following toxic insults to the kidney. The monoclonal antibodies prepared and the tests developed thus may provide direct diagnostic access to the renal papilla and allow, for the first time, early detection of papillary damage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002040050361
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  • 20
    Electronic Resource
    Electronic Resource
    Urinary antigens as markers of papillary toxicity. II: Application of monoclonal antibodies for the determination of papillary antigens in rat urine (1999)
    Springer
    Archives of toxicology 73 (1999), S. 233-245 
    Details
    ISSN: 1432-0738
    Keywords: Key words Rat papillary antigens ; Monoclonal antibodies ; Papillary toxicity ; Urinary markers ; ELISA-tests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have previously reported the preparation of monoclonal antibodies specific for antigens localized in the rat renal papilla. Three of the monoclonal antibodies reacting with antigens localized in papillary and cortical collecting duct epithelia were selected for the development of enzyme-linked immunosorbent assay (ELISA)-type assays. The papillary antigens (`PapA') determined in these tests were designated PapA1 (applying the monoclonal antibody PapX 5C10), PapA2 (applying the monoclonal antibody PapX 12F6), and PapA3 (applying the monoclonal antibody PapXI 3C7). Using these assays antigen excretion was determined in the urine of rats. Depending on the test compound used, the application route, and the dose, the observed antigen release patterns differed. Whereas after a single intraperitoneal application of 2-bromoethanamine or of propyleneimine an increased release of PapA1 but not of the two other antigens was observed oral application of bromoethanamine had minor effects. In contrast, both a single intraperitoneal application or repeated oral applications of indomethacin resulted in an increased release of all the three antigens. Daily application of ipsapirone in the diet or in drinking water resulted in significantly elevated urinary release of PapA1 which increased incrementally for the duration of the application. Release of PapA2 and PapA3 was not affected and remained in the normal range. These results show that with the tests developed changes in the rat renal papilla caused by xenobiotics can be detected early by urinary analysis and monitored during follow-up studies. Moreover, the different antigen release patterns obtained after application of the different compounds suggest a possible differing mode of action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002040050612
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