ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Crystals of 7α-hydroxysteroid dehydrogenase from E. coli, which is a tetramer in its active form, have been obtained by a hanging-drop vapor-diffusion method in the presence of the coenzyme NAD+. Crystals as large as 0.25 × 0.25 × 0.75 mm could be grown within a month at pH 8.5 with polyethylene glycol as precipitating agent. Preliminary X-ray crystallographic analysis revealed that they belong to one of the enantiomorphic space groups P41212 or P43212 with dimensions a = b = 81.66 and c = 214.6 Å, having two subunits in an asymmetric unit. The crystals diffract to at least 2.3 Å resolution.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S090744499501002X
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