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  • 1
    Electronic Resource
    Electronic Resource
    Density separation of alkylated coal macerals (1987)
    s.l. : American Chemical Society
    Energy & fuels 1 (1987), S. 280-286 
    Details
    ISSN: 1520-5029
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ef00003a010
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  • 2
    Electronic Resource
    Electronic Resource
    Development of two stage culture process by optimization of inorganic salts for improving catharanthine production in hairy root cultures of Catharanthus roseus
    Amsterdam : Elsevier
    Journal of Fermentation and Bioengineering 77 (1994), S. 57-61 
    Details
    ISSN: 0922-338X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0922-338X(94)90209-7
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  • 3
    Electronic Resource
    Electronic Resource
    Improvement of the catharanthine productivity in hairy root cultures ofCatharanthus roseus by using monosaccharides as a carbon source (1992)
    Springer
    Biotechnology letters 14 (1992), S. 695-700 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Sucrose, glucose, and fructose as carbon sources in culture medium were assessed in hairy root cultures ofCatharanthus roseus. The cultures preferentially consumed sucrose, resulting in about 40% (dry wt) higher growth rate. However, fructose enhanced the cathranthine yield about two-fold. The elevated yield was not seemingly ascribed to the higher osmolarity per unit weight of fructose than sucrose. A two stage culture using sucrose (1st) and fructose (2nd) improved volumetric yields of catharanthine about two-fold, i.e. 41 mg/l.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01021645
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  • 4
    Electronic Resource
    Electronic Resource
    Adaptive control of specific growth rate based on proton production in anaerobic fed-batch culture (1993)
    Springer
    Biotechnology letters 15 (1993), S. 511-516 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary This paper develops a practical and useful computer control scheme to control the specific growth rate as accurately as possible by measuring the released protons in anaerobic alcohol fermentation. In the case of current study, most of the released protons are due to the uptake of cationic ammonium ion via the conversion: NH4 + → NH3(cell) + H+. Correlating the Proton Production (PP) and the Proton Production Rate (PPR) with specific growth rate (μ) proved PP as a better measured variable. Using a simple adaptive control algorithm, μ was successfully controlled in a Zymomonas mobilis fed-batch culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00129328
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  • 5
    Electronic Resource
    Electronic Resource
    Biosynthesis of copolyesters consisting of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids from 1,3-butanediol or from 3-hydroxybutyrate by Pseudomonas sp. A33 (1995)
    Springer
    Applied microbiology and biotechnology 42 (1995), S. 901-909 
    Details
    ISSN: 1432-0614
    Keywords: Polyhydroxyalkanoates 3-hydroxybutyrate ; medium-chain-length 3-hydroxyalkanoates ; 1,3-butanediol Pseudomonas sp. A33 ; polyhydroxyalkanoate synthase polyhydroxyalkanoate depolymerase biodegradable polyesters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Pseudomonas sp. A33 and other isolates of aerobic bacteria accumulated a complex copolyester containing 3-hydroxybutyric acid (3HB) and various medium-chain-length 3-hydroxyalkanoic acids (3HAMCL) from 3-hydroxybutyric acid or from 1,3-butanediol under nitrogen-limitated culture conditions. 3HB contributed to 15.1 mol/100 mol of the constituents of the polyester depending on the strain and on the cultivation conditions. The accumulated polymer was a copolyester of 3HB and 3HAMCL rather than a blend of poly(3HB) and poly(3HAMCL) on the basis of multiple evidence. 3-Hydroxyhexadecenoic acid and 3-hydroxyhexadecanoic acid were detected as constituents of polyhydroxyalkanoates, which have hitherto not been described, by13C nuclear magnetic resonance or by gas chromatography/mass spectrometric analysis. In total, ten different constituents were detected in the polymer synthesized from 1,3-butanediol by Pseudomonas sp. A33:besides seven saturated (3HB, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, and 3-hydrohexadecanoate) three unsaturated (3-hydroxydodecenoate, 3-hydroxytetradecenoate and 3-hydrohexadecanoate) hydroxyalkanoic acid constituents occured. The polyhydroxyalkanoate synthase of Pseudomonas sp. A33 was cloned, and its substrate specificity was evaluated by heterologous expression in various strains of P. putida, P. oleovorans and Alcaligenes eutrophus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00191189
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  • 6
    Electronic Resource
    Electronic Resource
    Biosynthesis of copolyesters consisting of 3-hydroxybutyric acid and medium-chain-length 3-hydroxyalkanoic acids from 1,3-butanediol or from 3-hydroxybutyrate by Pseudomonas sp. A33 (1995)
    Springer
    Applied microbiology and biotechnology 42 (1995), S. 901-909 
    Details
    ISSN: 1432-0614
    Keywords: Key words Polyhydroxyalkanoates 3-hydroxybutyrate ; medium-chain-length 3-hydroxyalkanoates ; 1 ; 3-butanediol ; Pseudomonas sp. A33 ; polyhydroxyalkanoate synthase ; polyhydroxyalkanoate depolymerase ; biodegradable polyesters
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract  Pseudomonas sp. A33 and other isolates of aerobic bacteria accumulated a complex copolyester containing 3-hydroxybutyric acid (3HB) and various medium-chain-length 3-hydroxyalkanoic acids (3HAMCL) from 3-hydroxybutyric acid or from 1,3-butanediol under nitrogen-limitated culture conditions. 3HB contributed to 15.1 mol/100 mol of the constituents of the polyester depending on the strain and on the cultivation conditions. The accumulated polymer was a copolyester of 3HB and 3HAMCL rather than a blend of poly(3HB) and poly(3HAMCL) on the basis of multiple evidence. 3-Hydroxyhexadecenoic acid and 3-hydroxyhexadecanoic acid were detected as constituents of polyhydroxyalkanoates, which have hitherto not been described, by13C nuclear magnetic resonance or by gas chromatography/mass spectrometric analysis. In total, ten different constituents were detected in the polymer synthesized from 1,3-butanediol by Pseudomonas sp. A33 : besides seven saturated (3HB, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecanoate, 3-hydroxytetradecanoate and 3-hydrohexadecanoate) three unsaturated (3-hydroxydodecenoate, 3-hydroxytetradecenoate and 3-hydrohexadecanoate) hydroxyalkanoic acid constituents occurred. The polyhydroxyalkanoate synthase of Pseudomonas sp. A33 was cloned, and its substrate specificity was evaluated by heterologous expression in various strains of P. putida, P. oleovorans and Alcaligenes eutrophus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530050350
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  • 7
    Electronic Resource
    Electronic Resource
    Continuous production of enantiopure 1,2-epoxyhexane by yeast epoxide hydrolase in a two-phase membrane bioreactor (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 641-646 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A two-phase membrane bioreactor was developed to continuously produce enantiopure epoxides using the epoxide hydrolase activity of Rhodotorula glutinis. An aqueous/organic cascade, hydrophilic, hollow-fiber membrane bioreactor was used: (1) to carry out large-scale resolution of epoxides, (2) to continuously extract residual enantiopure epoxides from the aqueous phase, and (3) to separate inhibitory formed diol from the yeast cells contained in the aqueous phase. Dodecane was employed to dissolve-feed epoxide as well as to extract residual epoxide. 1,2-Epoxyhexane was used as a model substrate. By use of this membrane bioreactor, enantiopure (S)-1,2-epoxyhexane (〉98% enantiomeric excess) was obtained with a volumetric productivity of 3.8 g l−1 h−1. The continuous-production system was operated for 12 days and resulted in 38 g enantiopure (S)-1,2-epoxyhexane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530000451
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  • 8
    Electronic Resource
    Electronic Resource
    Resolution of 1,2-epoxyhexane by Rhodotorula glutinis using a two-phase membrane bioreactor (1999)
    Springer
    Applied microbiology and biotechnology 53 (1999), S. 7-11 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Large-scale resolution of epoxides by the yeast Rhodotorula glutinis was demonstrated in an aqueous/organic two-phase cascade membrane bioreactor. Due to the chemical instability and low solubility of epoxides in aqueous phases, an organic solvent was introduced into the reaction mixture in order to enhance the resolution of epoxide. A cascade hollow-fiber membrane bioreactor was used (1) to minimize the toxicity of organic solvents towards the epoxide hydrolase of R. glutinis, and (2) to remove inhibitory amounts of formed diol from the yeast cell containing aqueous phase. Dodecane was selected as a suitable solvent and 1,2-epoxyhexane as a model substrate. By use of this membrane bioreactor, highly concentrated (0.9 M in dodecane) enantiopure (〉 98% ee) (S)-1,2-epoxyhexane (6.5 g, 30% yield) was obtained from the racemic mixture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051606
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  • 9
    Electronic Resource
    Electronic Resource
    Selection of protoclones for high yields of indole alkaloids from suspension cultures ofCatharanthus roseus and the qualitative analysis of the compounds by LC-MS (1992)
    Springer
    Biotechnology techniques 6 (1992), S. 305-308 
    Details
    ISSN: 1573-6784
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary To produce economically important indole alkaloids by cell culture, we have selected protoclones ofCatharanthus roseus for high yields of catharanthine and ajmalicine. Protoplasts were enzymatically isolated from suspension-cultured cells. Protoclone VPC-10 produced catharanthine at 5.9 μg/g fresh wt of cells after 10 days of culture, although the original cell line did not produce it at a level detectable by HPLC. Under the same conditions, protoclone VPC-15 produced ajmalicine at 133.6 μg/g, which was about 3 times the productivity of the original cell line. In addition, the indole alkaloids were qualitatively confirmed by LC-MS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02439316
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  • 10
    Electronic Resource
    Electronic Resource
    Activator effects on urease kinetics (1976)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 22 (1976), S. 1106-1112 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: An experimental study of the effects of amino acid additives on the rates of urease kinetics showed that the degree of enhancement is sensitive to the relative levels of additives and substrate, and that enhancement can turn to inhibition at especially low concentrations of either arginine, DL-alanine, or glycine. Kinetic models developed to interpret these and prior literature data showed that all the data are consistent in the framework of the steady state model proposed but contradict the expectations that would follow from an equilibrium based treatment.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690220622
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