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  • 1
    Electronic Resource
    Electronic Resource
    Purification, characterization, and primary structure of a chitinase from Pseudomonas sp. YHS-A2 (2000)
    Springer
    Applied microbiology and biotechnology 54 (2000), S. 397-405 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A chitinase gene (chiA) from Pseudomonas sp. YHS-A2 was cloned into Escherichia coli using pUC19. The nucleotide sequence determination revealed a single open reading frame of chiA comprised of 1902 nucleotide base pairs and 633 deduced amino acids with a molecular weight of 67,452 Da. Amino acid sequence alignment showed that ChiA contains two putative chitin-binding domains and a single catalytic domain. Two proline-threonine repeat regions, which are linkers between catalytic and substrate-binding domains in some cellulases and xylanases, were also found. From E. coli, ChiA was purified 12.8-fold relative to the periplasmic fraction. The Michaelis constant and maximum initial velocity for p-nitrophenyl-N,N′-diacetylchitobiose were 1.06 mM and 44.4 μmol/h per mg protein, respectively. The purified ChiA binds not only to colloidal chitin but also to other substrates (avicel, chitosan, and xylan), but the binding affinity of avicel, chitosan, and xylan is around 10 times lower than that of colloidal chitin. The reaction of ChiA with colloidal chitin and chitooligosaccharides (trimer-hexamer) produced an end product of N,N′-diacetylchitobiose, indicating that ChiA is a chitobiosidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530000408
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  • 2
    Electronic Resource
    Electronic Resource
    A comparison of structure and magnetoresistance in Fe/(Ag–Cu) films (abstract) : The 40th annual conference on magnetism and magnetic materials (1996)
    [S.l.] : American Institute of Physics (AIP)
    Journal of Applied Physics 79 (1996), S. 6299-6299 
    Details
    ISSN: 1089-7550
    Source: AIP Digital Archive
    Topics: Physics
    Notes: Films of 12 Fe/(Ag–Cu) bilayers were prepared to have either granular or multilayer structures on glass substrates with a 50 A(ring) Cu buffer layer. A magnetic Fe layer of 15 A(ring) was deposited by e-beam evaporation, and a nonmagnetic Ag–Cu layer of 20 A(ring) by thermal coevaporation with various Ag/Cu ratio. As the nonmagnetic layer composition changed from Ag to Cu, roughness of the film surface varied from more than 100 A(ring) to less than 10 A(ring), measured with atomic force microscopy. Regularity of multilayer structure was evident in the Fe/Cu film from a superlattice peak at around 2theta=2.5 deg by Cu Kα x-ray diffraction. The peak weakened as the Ag content increased in the nonmagnetic layer, and completely disappeared at Ag/Cu(approximately-greater-than)1, which indicated that granular structure became dominant. Pendelloesung peaks at 2theta=1 to 2 deg had a similar composition dependency. The structural change was confirmed with cross-sectional transmission microscopy, too. The deposited structure is explained in terms of surface tension and wettability of each element. Increasing Cu content decreases the surface tension and increases the wettability of the nonmagnetic layer. Heat treated film structures are explained with identical terms. Change of magnetoresistance is discussed in part by connectivity of the nonmagnetic layer. From the discussion, a reasoning is given to the effect of annealing on the magnetoresistance of the films. © 1996 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.362043
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  • 3
    Electronic Resource
    Electronic Resource
    Enzyme induction and receptor-binding affinity of steroidal 20-carboxamides in rat hepatoma tissue culture cells
    Amsterdam : Elsevier
    Steroids 57 (1992), S. 313-318 
    Details
    ISSN: 0039-128X
    Keywords: (20R)-and (20S)-21-(N-substituted ; [^3H]thymidine uptake ; antedrug ; antiinflammation ; enzyme induction ; glucocorticoid ; hepatoma tissue culture ; receptor binding ; steroidal 20-carboxamides ; steroids ; tyrosine aminotransferase
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0039-128X(92)90049-F
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  • 4
    Electronic Resource
    Electronic Resource
    A role for glucagon-like peptide-1 in the central regulation of feeding (1996)
    [s.l.] : Nature Publishing Group
    Nature 379 (1996), S. 69-72 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We report that ICV administration of GLP-1 reduces food intake in fasted rats, with greater effect at higher doses (Fig. Ib). ICV injection of GLP-1 in rats at the beginning of the dark (feeding) phase also results in a profound decrease in feeding (Fig. la). When administered intraperitoneally up ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/379069a0
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  • 5
    Electronic Resource
    Electronic Resource
    Serum Creatine Kinase in Patients with Rheumatic Diseases (2000)
    Springer
    Clinical rheumatology 19 (2000), S. 296-300 
    Details
    ISSN: 1434-9949
    Keywords: Key words:Creatine kinase – Rheumatic diseases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: Serum creatine kinase (CK) activity is reduced in some conditions, including rheumatic diseases, but the aetiology and significance remain to be clarified. The aim of this study was to investigate relationships between serum CK activity and other muscle enzymes, muscle mass, renal function, steroid use and disease activity in patients with rheumatic diseases. Serum CK activity was measured in sera from 498 patients with rheumatic diseases: rheumatoid arthritis (RA, n= 145), systemic lupus erythematosus (SLE, n= 31), spondyloarthropathies (SpA, n= 35), polyarthralgia/arthritis (Poly, n= 74), miscellaneous group (MI, n= 46), and in non-inflammaroty arthropathies (NIA, n= 167) as controls. Serum CK level was significantly reduced in RA (45.4 ± 1.9 IU/l), SLE (46.4 ± 4.2 IU/l), SpA (64.7 ± 5.6 IU/l) and MI (63.4 ± 4.8 IU/l), but not in poly (70.2 ± 3.1 IU/l), compared to controls (78.9 ± 2.4 IU/l) (P 〈 0.05). CK values correlated with aspartate aminotransferase (AST), erythrocyte sedimentation rate (ESR), body mass index (BMI) and platelets (Plat) in RA; ESR and haemoglobin (Hb) in SLE, AST, ESR and Hb in SpA; lactate dehydrogenase (LDH), AST, ESR and Hb in Poly; LDH, AST, ESR, Hb and Ccr in MI; and LDH, AST and ESR in controls. In all patients with rheumatic diseases CK level was significantly corrrelated with LDH, AST, alanine aminotransferase (ALT), ESR, C-reactive protein (CRP) and BMI and prednisolone dose, but not with Ccr, age and disease duration. In conclusion, our data support the possibility that reduced CK activity is inversely correlated with inflammatory activity and correlated with other muscle enzymes, muscle mass and steroid use, but not with renal function, age and disease duration in rheumatic diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s100670070049
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  • 6
    Electronic Resource
    Electronic Resource
    Lipoprotein(a) and Lipids in Relation to Inflammation in Rheumatoid Arthritis (2000)
    Springer
    Clinical rheumatology 19 (2000), S. 324-325 
    Details
    ISSN: 1434-9949
    Keywords: Key words:Lipid’s – Lipoprotein(a) – Rheumatoid arthritis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract: The aim of this study is to evaluate whether lipoprotein(a) (Lp(a)) acts as the acute phase reactant and whether changes of lipids are related to inflammation in rheumatoid arthritis (RA). Lp(a) and lipids were measured after an overnight fast, before and after 14 days use of antiinflammatory agents and correlated with laboratory findings in 21 untreated RA patients and 19 healthy controls. Nine (42.3%) of 21 RA patients and 6 (31.6%) of 19 controls had high Lp(a) levels (〉 30 mg/dl) and the Lp(a) level was higher in RA patients compared with controls (27.1 ± 5.3 vs 19.0 ± 4.2 mg/dl) without significant difference (p 〉 0.05). There was no significant correlation between ESR and Lp(a) and lipids in RA patients except for HDL cholesterol (r=–0.563, p = 0.008). After antiinflammatory agent use for 14 days, change in ESR (ESRsample1–ESRsample2) was significantly and negatively correlated to changes in total and HDL cholesterols in RA patients. In conclusion, although Lp(a) tended to be higher in RA, we could not find a distinct acute phase pattern of Lp(a). But changes in total and HDL cholesterols were negatively correlated with inflammation in RA. Our data support the phenomenon that dyslipoproteinemia observed in RA is associated with inflammation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00011174
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  • 7
    Electronic Resource
    Electronic Resource
    Rheological study on poly-D(-)(3-hydroxybutyrate) and its blends with poly(ethylene oxide) (1995)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 35 (1995), S. 1636-1642 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Aspects of thermal, morphological, and rheological properties of biodegradable poly-D(-)(3-hydeoxybutyrate) (PHB) blended with poly(ethylene oxide) (PEO) have been studied. Thermal properties and morphology of the blends were examined by scanning electron microscopy and differential scanning calorimetry, respectively. A rotational theometer with parallel plate geometry was also adopted to investigate the rheological properties of these blends. In addition, dynamic ciscoelasticity was measured by a Rheovibron as functions of time and temperature. From these measurements, PHB and PEO were observed to be miscible in the melt state. In the case of the blend systen 80/20 PHB/PEO by weight, the vacant domains of the PHB were filled with PEO particles, and this morphological state enhanced the rheological properties. Furthermore, PHB and its blends were found to have high crystallinities, but to have unstable thermal behavior about Tm.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760352010
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