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  • 1
    Electronic Resource
    Electronic Resource
    Leucocytokinetic studies during viral hepatitis (1972)
    Springer
    European journal of pediatrics 113 (1972), S. 171-174 
    Details
    ISSN: 1432-1076
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Untersuchung der Knochenmarksfunktion mittels des Neutrophilen-Mobilisationstestes im Verlaufe viraler hepatitis bei 10 Patienten. In den ersten Erkrankungswochen wurden bei 6 Patienten pathologische Testergebnisse gefunden. Gleichlaufend mit der Heilung der Hepatitis normalisierten sich die Testergebnisse, oder anfänglich schon entsprechende Testresultate verbesserten sich. Als Erklärung wird eine reversible Knochenmarksdepression im Verlaufe viraler Hepatitiden angenommen.
    Notes: Abstract The neutrocyte mobilization test was performed in 10 children to investigate the bone marrow function during viral hepatitis. In the initial stage pathologic test results were obtained in 6 out of 10 patients. As the hepatitis improved the test responses normalized, or initially adequate responses increased. These findings are discussed as a manifestation of reversible bone marrow suppression during viral hepatitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00464620
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  • 2
    Electronic Resource
    Electronic Resource
    Calcium-Phosphor-Stoffwechseluntersuchungen an einem Fall von McCune-Albright-Syndrom mit Phosphatdiabetes (1974)
    Springer
    European journal of pediatrics 117 (1974), S. 47-61 
    Details
    ISSN: 1432-1076
    Keywords: McCune-Albright Syndrome ; Fibrous dysplasia of bone ; Hypophosphatemic rickets ; Secondary hyperparathyroidism ; Vitamin-D therapy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei einem 2 6/12 jährigen, familiär nicht belasteten Mädchen wird die Kombination eines Mccune-Albright-Syndroms mit einem Phosphatdiabetes beobachtet. Eine derartige Kombination ist in der Weltliteratur erst zweimal mitgeteilt worden. Nach kurzer Beschreibung der klinischen Symptomatik wird auf die ausgedehnten Skeletbefunde eingegangen. Pharmakologisch dosiertes Vitamin D3 führt zur Abheilung der rachitisch bedingten Veränderungen, während die fibröse Knochendysplasie dadurch radiologisch deutlicher hervortritt. Untersuchungen des Calcium-Phosphor-Stoffwechsels erbringen die einem Phosphatdiabetes entsprechenden Serum-und Harnbefunde; lediglich die alkalische Serumphosphatase zeigt ein Ausnahmeverhalten, da sie auch unter Vitamin D nicht zur Norm absinkt.
    Notes: Abstract The case of a 2 1/2-year-old girl with the unusual combination of fibrous dysplasia of bone and vitamin D-resistant rickets is described. Metabolic studies showed the evidence of phosphate diabetes with hypophosphatemia, normocalcemia and elevated alkaline phosphatase in the blood and extremely low calcium levels and high phosphate clearance that did not yield to calcium infusions in the urine. The X-ray pictures before and during high vitamin D therapy, which only made the rickets disappear, are discussed; they still showed fibrous dysplasia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00439023
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  • 3
    Electronic Resource
    Electronic Resource
    Culture studies of human pluripotent hemopoietic progenitors (1980)
    Springer
    Annals of hematology 41 (1980), S. 327-333 
    Details
    ISSN: 1432-0584
    Keywords: Pluripotent hemopoietic stem cells ; Mixed hemopoietic colonies ; Disorders of hemopoietic stem cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Conclusions Culture conditions are described that promote the growth of human pluripotent hemopoietic progenitors and facilitate their quantitation. These primitive cells form mixed colonies that may contain all elements of myeloid differentiation, including granulocytes, erythroblasts, megakaryocytes, and macrophages. Some mixed colonies contain, in addition to mature progeny, early progenitors that can be identified by their ability to form secondary hemopoietic colonies. The production of secondary mixed hemopoietic colonies by cells present in redispersed primary mixed hemopoietic colonies supports the view that some CFU-GEMM may self-replicate in culture, thus fulfilling one of the major operational requirements for pluripotent hemopoietic stem cells. Assessment of the proliferative state of CFU-GEMM under steady state conditions and in various clinical disorders suggests that the proliferative activity may reflect conditions that are associated with perturbations at the level of pluripotent progenitors. The recently observed increased plating efficiency of approximately 10 mixed colonies per 105 plated mononuclear cells will facilitate investigation of regulatory events at the level of pluripotent progenitors. With the use of putative stimulators, it might be feasible to modulate the cellular composition of mixed colonies and thus, identify mechanisms that determine the choice of pluripotent cells to self-replicate or to differentiate and mature into cells of specific phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01035488
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  • 4
    Electronic Resource
    Electronic Resource
    The role of CFU-GEMM in human hemopoiesis (1986)
    Springer
    Annals of hematology 53 (1986), S. 269-277 
    Details
    ISSN: 1432-0584
    Keywords: Pluripotent precursors ; CFU-GEMM ; Hemopoiesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The assay for CFU-GEMM has provided a measurement for pluripotent hemopoietic precursors in normal and abnormal hemopoiesis. While these cells are able to express the functional repertoire that includes not only myelopoiesis but also lymphopoiesis attempts to determine their self-renewal have shown little or no self-renewal capability. It is currently not known whether this observation reflects culture conditions favouring differentiation processes and surpressing self-renewal, or whether the observation made in culture truly reflects the potential of cells in vivo. Recent advances in molecular biology have lead to the identification of the genomic sequences of at least one of the hemopoietic growth factors thus confirming their importance as regulators.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320884
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  • 5
    Electronic Resource
    Electronic Resource
    Abstract (1992)
    Springer
    Annals of hematology 65 (1992), S. A1 
    Details
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02044602
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  • 6
    Electronic Resource
    Electronic Resource
    Stimulatory activity of PHA-LCM for normal human hemopoietic progenitors and leukemic blast cell precursors: Separation by isoelectric focusing (1981)
    New York, NY : Wiley-Blackwell
    Journal of Supramolecular Structure and Cellular Biochemistry 15 (1981), S. 41-48 
    Details
    ISSN: 0275-3723
    Keywords: hemopoiesis ; leukemia ; hemopoietic progenitors ; cell culture ; stimulatory molecules ; isoelectric focusing ; Chemistry ; Molecular Cell Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Medium conditioned by leukocytes in the presence of phytohemagglutinin (PHA-LCM) promotes growth of human hemopoietic progenitors (CFU-GEMM, BFU-E, CFU-C) and precursors of leukemic blast cells. PHA-LCM was separated by isoelectric focusing and each fraction tested with nonadherent cells of normal individuals as well as blast cells from two patients with acute myelogenous leukemia. Activity profiles for CFU-GEMM, BFU-E and CFU-C ranged from pH 5.0-6.5. The profile for activity stimulatory for leukemic blast cells was broader and ranged from pH 5.5-7.5. Although some overlap was observed, the main peaks of stimulatory activity for normally differentiating progenitors and precursors of leukemic blast cells were separable with respect to their isoelectric point.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jsscb.1981.380150105
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  • 7
    Electronic Resource
    Electronic Resource
    Flexible association of hemopoietic differentiation programs in multilineage colonies (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 121 (1984), S. 291-297 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Pluripotent hemopoietic progenitors lose potentialities during the process of differentiation. We have examined events that lead to lineage restriction by determining the cellular composition of 785 multilineage colonies grown from peripheral blood samples of glucose-6-phosphate-dehydrogenase (G-6-PD) heterozygous volunteers. Of these colonies, 762 contained only one isoenzyme type and were considered to be of clonal origin. A considerable heterogenity was observed. Some colonies were composed of cells belonging to two different lineages, while other colonies contained three or more different cell types. A small number of colonies consisted - in addition to myeloid cells - of T-lymphocytes. The variable association within individual colonies of members belonging to different hemopoietic lineages suggests a flexible determination and expression of differentiation programs by early progenitors.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041210205
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  • 8
    Electronic Resource
    Electronic Resource
    Segregation of precursors with high proliferative potential from their differentiated progeny in a myeloma cell line (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 136 (1988), S. 384-388 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Human myeloma colonies were grown from the peripheral blood of a patient with multiple myeloma who was unresponsive to any further therapy. The majority of primary myeloma colonies contained cells that were able to form secondary colonies upon replating and facilitated the establishment of a myeloma cell line (OCI-My5). The recloning procedure was repeated for colonies that contained the highest and lowest number of clonogenic progenitors. This approach allowed the selection of clonogenic cells with high self-renewal potential. Using a cell separation procedure based on differences in sedimentation velocity, clonogenic myeloma cells with high self-renewal were found to be smaller than the majority of clonogenic myeloma cells. Furthermore, cells with increasing size did not only display reduced self-renewal ability but showed significant increases in the content of mRNA transcripts for lambda light chains. These data were consistent with the view that clonogenic myeloma cells are heterogeneous with respect to self-replication and that they give rise to more differentiated progeny, as documented by increasing mRNA levels for the M-protein.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041360225
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  • 9
    Electronic Resource
    Electronic Resource
    Characterization of human megakaryocytic colony formation in human plasma (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 124 (1985), S. 67-74 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have analysed the contribution to megakaryocyte colony formation in methylcellulose made by human plasma, serum, media conditioned by phytohemagglutinin (PHA) stimulated leukocytes (PHA-LCM), erythropoietin (EPO) preparations, and platelets. The culture system was used as a bioassay for megakaryocyte colony stimulating activity (Meg-CSA) in plasma samples of patients with perturbed megakaryocytopoiesis. Preparations of heparinized platelet-poor plasma yielded the most consistent results. Platelet-poor plasma of normal subjects will at best facilitate the occasional growth of small megakaryocyte colonies. Colony frequency and size are reproducibly enhanced in the presence of PHA-LCM as a source of exogenous Meg-CSA. Commercially available EPO preparations may vary in their content of activities that influence megakaryocyte colony formation. Addition of these preparations to cultures that contain plasma and PHA-LCM usually does not enhance colony formation. In contrast to platelet-poor plasma, platelet rich plasma and serum are less supportive of megakaryocyte colony growth. It is suggested that this loss of activity may be related to the release of inhibitors by activated platelets or alternatively caused by absorption of activities by platelets. Plasma samples from patients with megakaryocytopoietic dysfunction may contain components that promote colony formation without addition of PHA-LCM or EPO. This phenomenon is consistently observed for patients with severe aplastic anemia and bone marrow transplant recipients after completion of their ablative preparative regimen.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041240112
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  • 10
    Electronic Resource
    Electronic Resource
    Regulation of interleukin-6 expression in the lymphoma cell line OCI-LY3 (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 148 (1991), S. 426-429 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Previously we described a cell line OCI-LY3 derived from a patient with non-Hodgkin's lymphoma. The cell line produced interleukin-6 (IL-6) mRNA and protein and demonstrated an autocrine pattern of growth for IL-6. Southern blot analysis of the IL-6 gene did not reveal any rearrangement. To determine whether the production of IL-6 by OCI-LY3 was due to subtle changes in the promoter of IL-6 or due to the expression of trans-acting factors chloramphenicol acetyltransferase (CAT) reporter constructs containing from -1, 180 to + 13 to -112 to + 13 of a normal IL-6 gene were elecroporated into the cell line. When these constructs are transferred into unstimulated fibroblasts, no CAT activity is seen; however, CAT activity is induced when the cells are stimulated with either IL-1α, lipopolysaccharide (LPS), or cyclic adenosine monophosphate (cAMP) analogues. When the cell line OCI-LY3 was transfected with these constructs, CAT activity was observed; it was not necessary to stimulate the cells with exogenous factors to observe this activity. No CAT activity was observed in a second lymphoma cell line, OCI-LY13.1, that does not produce IL-6. These results suggest that the constitutive production of IL-6 by the cell line OCI-LY3 is due to the presence of trans-acting factors that stimulate the expression of IL-6 and not due to a cis-acting mutation of the IL-6 promoter.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041480314
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