ISSN:
1530-0358
Keywords:
Laparoscopic surgery
;
Surgical trauma
;
Inflammatory response
;
Crohn's disease
;
Granulocyte elastase
;
Cytokines
;
C-reactive protein
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract PURPOSE: The aim of this study was to assess whether systemic proinflammatory cytokines (IL-6), anti-inflammatory cytokines (IL-4, IL-10), acute phase proteins (C-reactive protein), or granulocyte elastase are valuable indicators for determining the degree of surgical trauma after openvs. laparoscopic-assisted resections in Crohn's disease. METHOD: Eleven patients in each group (open and laparoscopic-assisted surgery) were matched for indication, surgical procedure, and Crohn's disease activity index. Serum IL-4, IL-6, and IL-10 were measured using enzyme-linked immunosorbent assay. Serum C-reactive protein was determined by immunoturbidimetric assay. Plasma granulocyte elastase was determined by immunoactivation immunoassay. Blood was sampled preoperatively, six hours after the operation, and at postoperative Days 1 to 5. RESULTS: IL-4 was not detectable in any sample analyzed. Serum IL-6 and IL-10 levels peaked postoperatively in both groups without significant differences between laparoscopic-assisted (185.6±54.1 pg/ml and 112.1±19.4 pg/ml, respectively; mean ± standard error of the mean) and open surgery (431.1±240.4 pg/ml and 196.7±56.5pg/ml, respectively). Serum C-reactive protein levels also rose postoperatively, with a peak on the second day, but showed similar values after laparoscopic-assisted (107.1±12.1 mg/l) and open (128.3±17.5 mg/l) surgery. Plasma granulocyte elastase levels peaked on the first and second postoperative day and were found elevated almost throughout the five-day observation period. Comparison between the groups revealed significantly (P〈0.02) lower values after laparoscopic-assisted (Day 1, 46.5±8.9 µg/l; Day 2, 41.9±5.9 µg/l) when compared with open surgery (Day 1, 89.7±13.8 µg/l; Day 2, 91.4±14). CONCLUSIONS: Serum IL-6 and IL-10 may not be ideal measures for evaluation of the degree of tissue trauma in laparoscopic-assisted and open resections in Crohn's disease, probably because of interference with disease-specific cytokine interactions. In contrast, granulocyte elastase has to be considered a strong marker discriminating the different severity of surgical trauma induced by laparoscopic-assistedvs. open resection in Crohn's disease.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02235052
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