ISSN:
1573-2657
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Isometric contraction (9–10°C) of skinned fibres from rat psoas muscle was elicited by photorelease of ATP (1.3–1.4 mM), and force (F), in-phase (I) and quadrature (Q) stiffness were monitored. The sinusoidal length change for the stiffness measurement was ≈ 0.09% fibre length and 200–1000 Hz. On photolysis,F andI initially fell, and then rose. The half-time of the rise ofI was shorter than that ofF. Contrary to the previous assumption,Q rose even earlier thanI. Although the time courses of the rise ofF andI were greatly affected by the presence of phosphate ion (Pi; 4 mM), its effect onQ was modest. We propose the following hypothesis: the rigor crossbridges, after binding ATP, initially enter a state characterized by highQ with lowI and none or littleF, and then, releasing Pi during passing the state with the increasedI, reach the final force generating state.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01738257
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