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  • 1
    ISSN: 1432-069X
    Keywords: Mycosis fungoides ; Histopathology ; Benign inflammatory dermatoses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The cutaneous infiltrate in mycosis fungoides (MF) is predominantly composed of T4-positive T-cells. Attempts to distinguish the early stages of this condition from benign inflammatory infiltrates using anti-T3, T4 and other T-cell-associated antibodies have hitherto been unsucessful. Recently a monoclonal antibody BE 2 has been described as selectively reacting against leukemic cells in patients with cutaneous T-cell lymphoma. To investigate whether the BE 2 antigen is differentially expressed in different stages of MF and benign dermatoses, thus facilitating diagnosis, especially of early MF, the reactivity of monoclonal antibody BE 2 against cutaneous infiltrates from such conditions was assessed. In the early stages of MF only a small number of reactive cells was present. In benign inflammatory infiltrates, especially in those that clinically and histologically were hardly distinguishable from early MF, BE 2 reactivity was essentially the same as in eczematous-stage MF. Lesions from plaque and tumor stage MF contained large numbers of BE 2-reactive cells. Our results indicate that expression of BE 2 is associated with the stage of a given MF lesion and is essentially identical in early MF and eczematous lesions with a similar histopathological appearance.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Human genetics 〈Berlin〉 20 (1973), S. 103-112 
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Um festzustellen, ob Ultraschall Chromosomenaberrationen zu induzieren vermag, wurden menschliches Venenblut und ankultivierte menschliche Lymphocyten in Teilung mit Ultraschall behandelt. Bei der Beschallung wurden Spezialgefäße benutzt, die Reflexionen ausschließen. Die Versuchsanordnung wurde jeweils so gewählt, daß eine Erhitzung des beschallten Mediums möglichst vermieden wurde. Zur Anwendung kam bei den Versuchen Dauerschall bis zu einer Intensität von 0,2 W/cm2 bei einer Frequenz von 2 MHz und bis zu einer Intensität von 35 W/cm2 bei einer Frequenz von 870 kHz. Außerdem wurde Impulsschall einer mittleren Intensität von 0,02 W/cm2 bei einer Frequenz von 2MHz benutzt (Pulsfolge 2000/sec, Pulsdauer 1 μsec). Anschließend wurden die Chromosomen aus den Lymphocyten dargestellt und auf numerische und strukturelle Aberrationen untersucht. Trotz der hohen angewendeten Intensitäten konnte weder eine chromosomenschädigende Wirkung noch eine Schädigung des Spindelapparates der Mitose festgestellt werden. Die Zellteilungsaktivität blieb unter den Beschallungen ebenfalls unbeeinflußt.
    Notes: Summary Human peripheral blood and cultured human lymphocytes undergoing the first mitosis were exposed to ultrasound in order to find out whether ultrasound can induce chromosome aberrations. Special vessels which prevented reflections were used for insonation. The experiments were carried out by a method avoiding any heating of the sonicated medium. During the investigations continuous ultrasound up to an intensity of 0.2 W/cm2 at a frequency of 2 MHz and up to an intensity of 35 W/cm2 at a frequency of 870 kHz was applied. In addition, pulsed sound of an average intensity of 0.02 W/cm2 at a frequency of 2 MHz was used (pulse sequence 2000/sec, pulse length 1 μsec). Afterwards chromosome cultures from lymphocytes were prepared and examined for numerical and structural aberrations. In spite of the high intensities applied no damaging effect on the chromosomes or the spindle were found. Mitotic activity was not affected by the insonation.
    Type of Medium: Electronic Resource
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