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  • 2000-2004  (3)
  • 1
    Electronic Resource
    Electronic Resource
    S-IV-02 
C2C12 Cells Differente in Three Dimensional Culture in Collagen Gel (2004)
    Oxford, UK; Malden, USA : Blackwell Science Inc
    Wound repair and regeneration 12 (2004), S. 0 
    Details
    ISSN: 1524-475X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Aim: The movements of the cell population are different between 2-D culture and 3-D culture. From the observation of morphology of skeletal muscle cell 3-D culture, We expect that skeletal muscle cells differentiation is accelerate in the collagen gel 3-D culture, and the proliferation is suppressed. The purpose of this study is to investigate the difference between 2-D culture and 3-D culture of C2C12 cells. Methods: C2C12 skeletal muscle cells are incubated following three difference conditions for 48 hours, plastic dish 2-D culture, collagen coated dish 2-D culture and collagen gel 3-D culture. The culture medium is Dulbecco's modified Eagle medium containing 10% fetal bovine serum and 1% penicillin/streptomycin. Collagens are removed by collagenase treatment and cells are homogenized. After centrifugation the top clear layer is used for CPK assay and protein development analysis by Western blotting Results: After 48 hour incubation, we observed cell morphology by a phase contract microscope. Cell fusion was observed in collagen gel 3-D culture. The fusion cells have many nucleus in the cytoplasm called synthetium. But in plastic dish 2-D culture and in collagen coated dish 2-D culture synthetiums were not observed and cells were mononuclear and monolayer. Cell prolieration was suppressed in collagen gel 3-D culture. CPK activity was five times activated in collagen gel 3-D culture than in plastic dish 2-D culture. Conclusions: We suggest skeletal muscle cells C2C12 are activate differentiation by collagen gel 3-D culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1067-1927.2004.abstractj.x
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  • 2
    Electronic Resource
    Electronic Resource
    Postmortem absorption of dichloromethane: a case study and animal experiments (2000)
    Springer
    International journal of legal medicine 114 (2000), S. 96-100 
    Details
    ISSN: 1437-1596
    Keywords: Key words Blood ; Dichloromethane ; Poisoning ; Postmortem change ; Tissue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract A case of accidental death after occupational exposure to an atmosphere containing dichloromethane (DCM) is reported. The concentrations of DCM in the blood and tissues of a 40-year-old man who died while observing an industrial washing machine filled with DCM vapour were blood 1660 mg/l, urine 247 mg/l, brain 87 mg/ kg, heart muscle 199 mg/kg and lungs 103 mg/kg which are 3–7 times higher than previously reported fatal levels. The body was left undiscovered in the machine filled with DCM vapour for about 20 h. The present study was designed to determine whether all the DCM detected in the tissues and body fluids had been inhaled while alive using rats as the experimental model. The concentrations of DCM in the tissues and body fluids of a rat that died from DCM poisoning and was left for 20 h in a box containing DCM vapour were the same as those in the tissues and body fluids of a rat that had died from an injected overdose of barbiturates and had then been placed in the DCM box in a similar manner. Moreover, the concentrations of DCM in the tissues and body fluids of the carcasses that were exposed to the DCM vapour increased gradually throughout the period of exposure. These findings imply that DCM is able to penetrate the tissues and body fluids of rat carcasses through a route other than inhalation such as through the skin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004140000141
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Oxidation behavior of Mo≤5Si3C≤1 and its composites (2000)
    Springer
    Journal of materials science 35 (2000), S. 863-872 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The oxidation behavior of Mo≤5Si3C≤1 and its composites was studied in air over the temperature range of 500°C–1600°C. Experiments revealed poor oxidation resistance of monolithic Mo≤5Si3C≤1 at high temperature. The oxidation was quite rapid at 1200°C and above, resulting in complete oxidation of specimens in a short time. The addition of 2.0 wt% boron was found to produce a Mo≤5Si3C≤1 composite with three other phases of MoB, MoSi2, and SiC, and showed remarkable improvement in oxidation resistance. The mechanism for the improvement was attributed to the viscous sintering of the scale to close the pores formed during the initial oxidation period. Oxidation tests were also conducted on SiC-Mo≤5Si3C≤1 composite at 800°C, 1300°C and 1600°C for more than 100 hours. The oxidation resistance of the composite was found to be very good. The results demonstrate that, though oxidation resistance of monolithic Mo≤5Si3C≤1 is far insufficient for high-temperature applications, boron-modification and/or composites with SiC are viable methods to improve oxidation resistance to a practically acceptable level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004786005333
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    Paper (German National Licenses)
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