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  • 1
    Electronic Resource
    Electronic Resource
    Emulsifying and Surface Properties of Wheat Gluten under Acidic Conditions (2001)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 66 (2001), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The solubility of wheat gluten was greatly improved at pH 4 or lower where it showed good emulsifying activity. This might be due to its high surface activity in the acidic pH range and the formation of a stable protein film surrounding the oil droplets. Among the major gluten proteins, gliadins showed higher surface activity than glutenins. The content of glutenins in the adsorbed protein film was higher than that of gliadins, and glutenins are likely to have been adsorbed more tightly than gliadins. These results suggest that gluten proteins exhibit complex behavior, such as adsorption/desorption/displacement/rearrangement during the adsorption process in a gluten-stabilized emulsion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.2001.tb16116.x
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  • 2
    Electronic Resource
    Electronic Resource
    Mutation screening of interferon regulatory factor 1 gene (IRF-1) as a candidate gene for atopy/asthma (2000)
    Oxford BSL : Blackwell Science Ltd
    Clinical & experimental allergy 30 (2000), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: IL-4 gene cluster on chromosome 5 contains several candidate genes for atopy and asthma. Several independent studies have shown evidence for linkage between the markers flanking IL-4 gene cluster and asthma and/or asthma-related traits. Interferon regulatory factor 1 (IRF-1) is located approximately 300 kb telomeric to IL-4 and recent study reveals that IRF-1 deficiency results in an elevated production of Th2-related cytokines and a compensatory decrease in the expression of native cell- and Th1-related cytokines.〈section xml:id="abs1-2"〉〈title type="main"〉ObjectiveTo determine if there are any mutations associated with the development of atopy and asthma present in the coding exons and 5′ flanking region of the IRF-1 gene.〈section xml:id="abs1-3"〉〈title type="main"〉Methods and resultsWe have screened the promoter and coding regions of the IRF-1 gene in atopic asthmatics and controls by SSCP method. We found three novel nuclear variants (the −300G/T and 4396 A/G polymorphisms and the 6355G 〉 A rare variant) in the IRF-1 gene. No variants causing amino acid alterations of IRF-1 were detected. The −300G/T polymorphism was in nearly complete linkage disequilibrium with the 4396 A/G polymorphism. An association between the 4396 A 〉 G polymorphism and atopy/asthma was examined by transmission disequilibrium test in 81 asthmatic families. Either of 4396 A or 4396G alleles was not significantly preferentially transmitted to atopy- or asthma-affected children.〈section xml:id="abs1-4"〉〈title type="main"〉ConclusionThe IRF-1 gene is less likely to play a substantial role in the development of atopy and asthma in the Japanese population.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2000.00916.x
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic analysis of tissue culture traits in barley cv. ‘Lenins’ (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The barley cultivar ‘Lenins’ was found to be a genotype showing high shoot regeneration ability in cultures derived from immature embryos. Five cultivars different from ‘Lenins’ in shoot regeneration ability were reciprocally crossed with ‘Lenins’ and the inheritance of tissue culture traits was investigated. F2 plants showed continuous distributions in callus growth and percentage of shoot regeneration, suggesting that these traits were controlled by polygenes. The F2 population, derived from a cross between ‘Lenins’ and ‘6721′, showed a monogenic segregation for the number of regenerated shoots, and the segregation ratio fitted 1:2:1. Tissue culture traits of ‘Lenins’ were controlled by several genes, whereas the number of regenerated shoots related to the efficiency of shoot regeneration is controlled by one major gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00822.x
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular and chemical analysis of β-amylase-less mutant barley in Tibet (2000)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 119 (2000), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The molecular and biochemical aspects of Tibetan β-amylase-less mutant barley were investigated. An enzyme assay showed that there were eight β-amylase-less mutant lines in the Tibetan landrace barley. Following Southern analysis and polymerase chain reaction, it is suggested that these mutants result from the same insert mutation in the latter part of the β-amylase structural gene. The α-glucosidase activity level of the mutant line was high in the initial period of germination, and the glucose ratio in the sugar conformation was remarkably high in the germinated seed of the mutant line. These results suggest that this β-amylase-less mutant line could be a new breeding material for beer production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2000.00502.x
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  • 5
    Electronic Resource
    Electronic Resource
    A variety of wheat tolerant to deep-seeding conditions: elongation of the first internode depends on the response to gibberellin and potassium (2001)
    Oxford, UK : Blackwell Science Ltd
    Plant, cell & environment 24 (2001), S. 0 
    Details
    ISSN: 1365-3040
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: The addition of gibberellin A3 (GA3) to culture media induced a dramatic elongation of the first internode in Hong Mang Mai, a wheat variety tolerant to deep-seeding conditions. The length of the first internode in the GA3-treated seedlings reached approximately 45 cm, which is twice as long as that of the control, whereas first internodes of other varieties were 3 to 12 cm long and hardly affected by GA3. The level of endogenous gibberellins (GA) in Hong Mang Mai was not greater than that of other wheat varieties. The expression of GAMyb, a transcription factor, was abundant in the first internode and substantially increased by GA3 application in Hong Mang Mai, compared with other wheat varieties. These results suggest that the first internode of Hong Mang Mai is more sensitive to GA in inducing strong elongation. The presence of potassium in the culture media was indispensable for the first internode elongation. Application of GA3 enhanced the uptake of potassium in Hong Mang Mai. Higher sensitivity of the first internode to GA could cause strong elongation by increasing the amount of osmotic solute, which plays an important role in the tolerance mechanism of Hong Mang Mai to deep-seeding conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-3040.2001.00688.x
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  • 6
    Electronic Resource
    Electronic Resource
    Single nucleotide polymorphism genotyping of the barley waxy gene by polymerase chain reaction with confronting two-pair primers (2004)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 123 (2004), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: A high-throughput single nucleotide polymorphism (SNP) genotyping procedure was developed to select amylose-free barley mutants whose waxy genes had a C- to T-base substitution in exon 5, which converted Gln-89 of the wild-type gene into a termination codon. An F2 population carrying an amylose-free waxy gene was checked for segregation. Polymerase chain reaction with confronting two-pair primers (PCR-CTPP) produced allele-specific PCR products that have different sizes and are inherited in a co-dominant manner. Two alleles of the barley waxy gene with SNP were correctly identified in parental strains using the PCR-CTPP procedure. Segregation of the SNP as detected by PCR-CTPP in an F2 population fitted the expected 1:2:1 ratio. The PCR-CTPP procedure can provide a time saving and cost-effective alternative to derived cleaved amplified polymorphic sequence in marker-assisted selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2004.00970.x
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  • 7
    Electronic Resource
    Electronic Resource
    Barley lines showing prominent high green shoot regeneration in cultures derived from immature embryos (2003)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 122 (2003), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Eighty-four cultivars of barley (Hordeum vulgare) and 95 wild strains (82 of H. spontaneum and 13 of H. agriocrithon) were surveyed for the production of callus, callus growth, and shoot regeneration in cultures derived from immature embryos. All cultivars except for ‘Turkey 381′, induced calli from more than 90% of embryos. On the other hand, the wild lines showed a large variation in the percentage of callus induction from 0 to 100%. Among the cultivars, those with the brittle rachis genotype, bt Bt2, on chromosome 3H, regenerated shoots with a significantly higher percentage than the cultivars with the Bt bt2 genotype. Green shoots were produced in a higher ratio (0.84) in the cultivars than in the wild lines (0.52). Among the lines examined,‘Lenins’ regenerated shoots efficiently (90.4%) and produced the highest number of calli with green shoots per embryo (4.77) followed by ‘Golden Promise’ (3.15). Examination of callus growth and shoot regeneration from embryos at different developmental stages revealed that scutellum development affected the quantity and quality of callus and shoot regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2003.00823.x
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  • 8
    Electronic Resource
    Electronic Resource
    Normal Development of the Gut-Associated Lymphoid Tissue Except Peyer's Patch in MyD88-Deficient Mice (2003)
    Oxford, UK : Blackwell Science Ltd
    Scandinavian journal of immunology 58 (2003), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: MyD88 is a key adaptor molecule for signalling via Toll-like receptors (TLRs) and the response to gut commensal microbes. To investigate the role of TLRs/MyD88 pathway in the development of the gut-associated lymphoid tissue (GALT), we examined the development of Peyer's patches (PPs) and cryptopatch (CP), and also one of effector compartment, intraepithelial lymphocyte (IEL) in MyD88–/–, TLR2–/– and TLR4–/– mice. In MyD88–/– mice, the organogenesis of PPs was not disturbed. However, PPs in 2-week-old MyD88–/– mice were significantly smaller than those in MyD88+/– mice. Also, in 2-week-old TLR4–/–, but not TLR2–/– mice, PPs did not develop rapidly. The development of PPs in MyD88–/– and TLR4–/– mice was completely recovered in 10 weeks. PP cells from MyD88–/– mice showed significant decrease in proliferation when stimulated with lipopolysaccharide. The development of CP and IEL was also normal in 10-week-old MyD88–/– mice. These results suggest that the TLRs/MyD88 pathway might be involved in the development of PPs only at early postnatal stage, and TLRs/MyD88-independent signalling is critically involved in the development of GALT in adult mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.2003.01346.x
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  • 9
    Electronic Resource
    Electronic Resource
    Pancreatic cancer: current status of treatment and survival of 16 071 patients diagnosed from 1981–1996, using the Japanese National Pancreatic Cancer Database (2000)
    Springer
    International journal of clinical oncology 5 (2000), S. 153-157 
    Details
    ISSN: 1437-7772
    Keywords: Key words Pancreatic cancer ; National database ; Multidisciplinary treatment ; Survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background. The Japanese Pancreas Society Registry Committee obtained data on 16 071 patients with pancrea-tic cancer diagnosed from 1981 to 1996. This is the largest multi-institutional retrospective study of the Japanese experience to describe the extent of disease and survival. Methods. Data were summarized according to age and sex and location of the disease. Survival analysis, using the Kaplan-Meier method, was performed according to surgical stage and therapeutic categories. Results. The male : female ratio was 1.6 : 1. The peak age was in the patients' sixties for both sexes. Forty-five per-cent of the primary tumors were located in the head of the pancreas. The survival of the patients depended on the histological diagnosis and surgical stage. The most com-mon histology, invasive ductal carcinoma, had the worst prognosis, and the 5-year survival of patients with stage I, II, III, IVa, and IVb disease was 66%, 55%, 21%, 11%, and 6% respectively. At the time of presentation, only 2.5% of the patients had stage I disease, while more than 70% had stage IV. Conclusions. Improvements in early detection strategies and more effective chemical or biological therapeutic agents, together with pancreatectomy, tested in well organized randomized prospective trials, will be the only way to cure this fatal disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00012030
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  • 10
    Electronic Resource
    Electronic Resource
    The distribution, organization and evolution of two abundant and widespread repetitive DNA sequences in the genus Hordeum (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 169-176 
    Details
    ISSN: 1432-2242
    Keywords: Key words Barley ; Hordeum ; In situ hybridization ; Phylogeny ; Tandemly repeated DNA sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The genomic organization and chromosomal distributions of two abundant tandemly repeated DNA sequences, dpTa1 and pSc119.2, were examined in six wild Hordeum taxa, representing the four basic genomes of the genus, by Southern and fluorescence in situ hybridization. The dpTa1 probe hybridized to between 30 and 60 sites on the chromosomes of all five diploid species studied, but hybridization patterns differed among the species. Hybridization of the pSc119.2 sequence to the chromosomes and Southern blots of digested DNA detected signals in Hordeum bulbosum, Hordeum chilense, Hordeum marinum and Hordeum murinum 4x, but not in Hordeum murinum 2x and Hordeum vulgare ssp. spontaneum. A maximum of one pSc119.2 signal was observed in the terminal or subterminal region of each chromosome arm in the species carrying this sequence. The species carrying the same I-genome differed in the presence (Hordeum bulbosum) or absence (Hordeum spontaneum) of pSc119.2. The presence of pSc119.2 in the tetraploid cytotype of Hordeum murinum, but its absence in the diploid cytotype, suggests that the tetraploid is not likely to be a simple autotetraploid of the diploid. Data about the inter- and intra-specific variation of the two independent repetitive DNA sequences give information about both the interrelationships of the species and the evolution of the repetitive sequences.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050023
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