Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Differential modulation of interleukin-1α (IL-1α) and interleukin-1β(IL-1β in human epidermal keratinocytes by UVB (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Experimental dermatology 3 (1994), S. 0 
    Details
    ISSN: 1600-0625
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Abstract Conflicting reports exist concerning ultraviolet-B (UVB) effects on keralinocyte (KC) interleukin-l (IL-1) expression. To clarify the modulatery effects of UVB on IL-1, the following study was undertaken. Normal human epidermal KCs cultured in a standard low Ca2+ and serum-free medium were irradiated in quiescent phase with UVB. In this study, we used semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) to determine the mRNA level of interleukin-1α (IL-1α) and interleukin-1β (IL-β). After exposure to 100 or 300 J/m2 UVB, a transient increase in mRNA levels was observed within I hour for IL-1α and 3 to 6 h for IL-β Following this transient induction, mRNA levels for both IL-1α and IL-1β returned to steady-state levels after 100 J/m2. After 300 J/m2 irradiation, IL-1α and IL-1β levels were downregulated compared to unirradiated cultures at 24-h post-irradiation. The half-life for IL-1α and IL-1β was estimated using actinomycin D treatment. Both IL-1α and IL-1β mRNAs half-lives (t1/2) decreased faster in irradiated cells (t1/2 = 30 minutes for IL-1α and 2 h for IL-1β) compared to unirradiated cells (t1/2= 1 h and 4 h, respectively). These results suggest that IL-1α and IL-1β mRNA expression are differentially regulated by UVB. In contrast to down-regulation of mRNA levels, a significant increase in IL-1α protein levels, measured by ELISA. was observed in culture supernatant from 6 h to 24 h after 300 J/m2 UVB irradiation. Cycloheximide treatment did not abrogate this increase in IL-1α protein level. Since this dose of UVB irradiation decreased the stability of IL-1α and IL-1β mRNA, this suggests that the release of IL-1α after UVB irradiation was due to leakage from UVB-damaged cells and not from de novo protein synthesis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0625.1994.tb00263.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Circulating CD34+ hematopoietic progenitors in the harvesting peripheral blood stem cells; enhancement by recombinant human granulocyte colony-stimulating factor (1992)
    Springer
    Biotherapy 5 (1992), S. 131-136 
    Details
    ISSN: 1573-8280
    Keywords: autotransplantation ; CD34 ; granulocyte colony-stimulating factor ; peripheral blood stem cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The number of circulating progenitor cells increases during the period of hematopoietic recovery following myeloablative therapy. These progenitor cells were used for autologous transplantation in order to reconstitute hematopoiesis. As an indicator of the circulating progenitor cells, the number of granulocyte-macrophage colony forming units (CFU-GM), which is measured by means of a long-term cell culture, has been widely used. Recently, a cell surface marker, CD34, which can easily be measured by means of flowcytometry, was found to represent immature hematopoietic progenitor cells, which are very close to stem cells. Therefore, the relationship between the number of CD34 positive cells (CD34+ cells) and the number of CFU-GM in the peripheral blood following chemotherapy was studied in 9 patients selected to undergo autotransplantation. The number of peripheral blood CD34+ cells was found to be significantly correlated with that of CFU-GM (r = 0.81). When four out of 9 patients received recombinant human granulocyte-colony stimulating factor (rG-CSF) administration, a significant increase in the release of peripheral blood CD34+ cells as well as peripheral blood CFU-GM was observed (P〈0.01). Thus, the measurement of CD34+ cells is useful for predicting the number of circulating CFU-GM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02171698
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    CD7-positive acute myeloid leukemia: further evidence of cellular immaturity (1992)
    Springer
    Journal of cancer research and clinical oncology 118 (1992), S. 386-388 
    Details
    ISSN: 1432-1335
    Keywords: AML ; CD7 ; CD34 ; Flow cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Among 63 cases of acute myeloid leukemia (AML), 14 were found to express the CD7 antigen, a cell surface marker usually found at an early stage during T lineage differentiation. The CD7-positive AML cases consisted of 5 cases of M1, 3 cases of M2, 3 cases of M4, 1 case of M5, 1 case of M6 and 1 case of M7. Among these 63 cases, the proportion of blast cells expressing the CD34 antigen was examined. The proportion of CD34-stained cells among the CD7-positive AML cases, although varying, was significantly larger than that among the CD7-negative AML cases (P〈0.05). As the CD34 antigen was expressed on hematopoietic progenitor cells and was considered to reflect an early hematopoietic stage, the high proportion of cells expressing CD34 among the CD7-positive AML cases may support the notion that CD7-positive AML cells are immature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01294444
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Analysis of a human DNA excision repair gene involved in group A xeroderma pigmentosum and containing a zinc-finger domain (1990)
    [s.l.] : Nature Publishing Group
    Nature 348 (1990), S. 73-76 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] We cloned the XPAC gene, and detected 1.0-1.1 kilobase (kb) XPAC mRNA in mouse cells, and 1.3-1.4 kb and 1.0-1.1 kb XPAC mRNAs in normal human cells1. To obtain human and mouse cDNAs corresponding to these mRNAs, we screened pcD2 human and mouse expression cDNA libraries2 using one of the ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/348073a0
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Gene expression of cytokines suppressing hematopoietic progenitor cells in lymphoid malignancies (1991)
    Springer
    Journal of cancer research and clinical oncology 117 (1991), S. 359-363 
    Details
    ISSN: 1432-1335
    Keywords: Lymphoid malignancies ; Tumor necrosis factorα ; Lymphotoxin ; Transforming growth factorβ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The expression of cytokine genes for tumor necrosis factorα (TNFα), lymphotoxin and transforming growth factorβ (TGFβ), all of which are known to suppress normal hematopoiesis, was investigated in 32 patients with lymphoid malignancies using Northern blot analysis. Messenger RNA (mRNA) for TNFα, lymphotoxin and TGFβ was detected in 9 cases, 2 cases and 7 cases, respectively. When the relationship between cytokine gene expression and surface phenotype was analyzed, the expression of CD19 correlated significantly with expression of the TNFα gene (P〈0.05). This suggests that B cell malignancies are likely to produce TNFα. When the hematological parameters of patients expressing and not expressing the gene were compared, the expression of TNFα mRNA was found to correlate with more profound anemia in acute lymphoblastic leukemia (P〈0.05). Both granulocyte and platelet counts were lower in patients expressing TNFα mRNA; however, the decreases were not significant. Neither lymphotoxin nor TGFβ gene expression correlated significantly with any hematological parameter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01630720
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Scintigraphic detection of neural-cell-derived small-cell lung cancer using glioma-specific antibody (1994)
    Springer
    Journal of cancer research and clinical oncology 120 (1994), S. 259-262 
    Details
    ISSN: 1432-1335
    Keywords: Small-cell lung cancer ; Scintigraphy ; Monoclonal antibody
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Radiolabeled GA-17, a murine monoclonal antibody that reacts specifically with glioma cells, bound to a small-cell lung cancer (SCLC) cell line NCI-H69 derived from neural cells, both in vitro and in vivo. The affinity constant of GA-17 F (ab′)2 fragment binding to NCI-H69 was 1.02×108/M while that to the glioma cell line U87MG was 1.22×108/M. Iodine-125-labeled GA-17 F (ab′)2 fragments injected i.v. localized well in NCI-H69 cells xenografted in nude mice. The percentage of the injected dose per gram accumulated in the xenografted tumor was 6.87±1.34%g−1 (mean±SD,n=5) 24 h after injection. On the other hand, control monoclonal F (ab′)2 fragments accumulated in the xenografted tumor at 0.75±0.30%g−1. The tumor-to-blood ratio was 1.8 for NCI-H69, while that of control F (ab′)2 was 0.60. In conclusion, the radiolabeled GA-17 F (ab′)2 fragment is expected to be useful clinically to visualize the small-cell lung cancer and in radioimmunotherapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01236381
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Analysis of acute myeloid leukemia cells by flow cytometry, introducing a new light-scattering classification (1994)
    Springer
    Journal of cancer research and clinical oncology 120 (1994), S. 553-557 
    Details
    ISSN: 1432-1335
    Keywords: Acute myeloid leukemia ; Light-scattering classification ; Immunophenotyping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A combined flow-cytometric evaluation of light scattering and the immunophenotype of acute myeloid leukemia (AML) cells from 71 newly diagnosed consecutive patients was conducted. Light-scattering characteristic of AML cells examined by flow cytometry and multiple surface markers were also analyzed using the same samples, to enable a comparison with the French-American-British (FAB) classification. Our AML cases could be classified into three light-scattering classification (LSC) types according to their physical properties on flow cytometry. These were type A, where forward light scattering (FSC) of the leukemic cell population was larger than that of lymphocytes, while side light scattering (SSC) was the same or larger than that of lymphocytes but smaller than that of monocytes; type B, where FSC of the leukemic cell population was larger than that of lymphocytes and SSC spread toward that of monocytes; and type C, where both FSC and SSC of the leukemic cell population spread beyond those of monocytes. Although a clear relationship between the FAB classification and LSC classification by the light-scattering profile of AML was not established, we observed the following findings. The majority of cases were classified as type A (58%), while type B comprised 25% and type C comprised 17%. While CD7 expression on AML cells is considered to be an immature characteristic, CD7 was expressed more frequently among LSC type A cases. Furthermore, all but one of the FAB M1 cases were classified as type A. On the other hand, CD7 was not expressed on type C leukemic cells. The percentage of cases in which more than 60% of leukemic cells possessed another immature surface antigen, CD 34ö, was 13/18 (72%) among FAB M1 cases, much higher than among FAB M2 (35%) or FAB M4 (27%) cases. A negative correlation was observed between mature antigen CD33 and CD34 among the FAB M2 cases. The frequency of CD7 expression was 25% among the total cases, and CD7-positive cases were frequent among FAB M1 and M2, but not among FAB M3 cases. These findings concerning LSC and immunophenotyping indicate that the scattergram pattern analysis may contribute towards more precise immunophenotyping, in that it reflects the maturation stage of each AML case.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01221034
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Restoration of proliferating cell nuclear antigen (PCNA) complex formation in xeroderma pigmentosum group a cells following cis-diamminedichloroplatinum (II)-treatment by cell fusion with normal cells (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 152 (1992), S. 639-645 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We examined the role of the factor deficient in xeroderma pigmentosum group A (XP-A) cells in the formation of proliferating cell nuclear antigen (PCNA) complex with DNA in the DNA repair process in human fibroblasts following cisdiamminedichloroplatinum (CDDP)-treatment. Immunofluorescence staining after methanol fixation was used to detect the PCNA complex formation. When quiescent normal cells were PCNA-stained at 3 h after 100 μM CDDP treatment for 1 h, almost all nuclei of the cells showed a punctuated staining pattern. On the other hand, nuclei of XP-A cells were not stained. These results were the same with the findings following 10J/m2 of ultraviolet light (UV)-irradiation. The quantitative analysis of the PCNA immunofluorescence intensity of normal cells revealed that the mean intensity was increased by 4.8 times by the CDDP-treatment and 6.1 times by the UV-irradiation, compared with that of untreated cells. The intensities among nuclei ranged widely in both treatments. In contrast, the mean intensity was not increased in XP-A cells by the same treatments. However, when XP-A cells were fused with normal cells with polyethylene glycol (PEG) treatment, the nuclei of the XP-A cells showed positive PCNA-staining following CDDP-treatment or UV-irradiation in almost all cases. These results suggest that the PCNA complex formation may play a role in the DNA repair process after the step where the factor deficient in XP-A cells is involved following CDDP-treatment as well as following UV-irradiation. © 1992 Wiley-Liss, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041520324
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...