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  • 1980-1984  (4)
  • 1
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Zusammenfassung Ein Zusatz von Kuhmilch zu Schaf-und/oder Ziegenmilch bzw. -käse kann nach Carbamylierung der Proteine durch Gegenstromelektrophorese auf Agarosegelen im Bereich von 0,1 bis 1 % als untere Grenze bei Milch bzw. von 0,1 bis 2% Kuhmilchkäse als untere Grenze bei Käse nachgewiesen werden. Hierzu wird ein spezifisches Antiserum gegen Rinder-Immunglobulin vom Typ G eingesetzt. Nur bei einer wesentlichen Überschreitung der üblichen Pasteurisationsbedingungen für Milch oder bei sehr starker Proteolyse während der Käsereifung kann ein sicherer Nachweis nicht gewährleistet werden.
    Notes: Summary The addition of cow milk to sheep or goat milk respectively — sheep or goat cheese can upon carbamylation of the proteins, be detected by counter current electrophoresis on agarose gel. Lower limits of detection are 0.1–1% in milk and 0.1–2% in cheese. To accomplish this an antiserum specific to bovine immunoglobulin type G is used. Only, when the usual pasteurisation conditions for milk have been substantially exceeded, or upon very strong proteolysis during ripening of the cheese, certain detection cannot be guaranteed.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1438-2385
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Zusammenfassung Mit Hilfe der trägerfreien Elektrophorese nach Hannig gelingt es, zuvor nicht antigene Maisproteine so schonend zu isolieren und anzureichern, daß eine erfolgreiche Immunisierung durchge führt werden kann. Es wird dabei angenommen, daß es zu einer Abtrennung von die Immunisierung störenden Stoffen kommt. Mit den erhaltenen Antisera lassen sich bei Bier verwendete Rohfruchtproteine in immunologischen Methoden nachweisen.
    Notes: Summary By means of free flow electrophoresis according to Hannig it is possible to isolate and enrich previously not antigenic proteins ofZea mays in a very careful manner, so that afterwards a successful immunisation can be carried out. It is assumed that the separation procedure of the electrophoresis eliminates some interfering substances from the protein extract e.g. carbohydrates. With the thus obtained antiserum added raw fruit proteins from corn in beer sud could be detected in the final product by immunological method.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Fresenius' Zeitschrift für analytische Chemie 317 (1984), S. 599-600 
    ISSN: 1618-2650
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 0173-0835
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: The identification of turkey meat in food products has been accomplished using specific antisera against turkey troponin T. This protein (Mr 37 000) was extracted from fresh muscle tissue and isolated by a procedure that included free-flow electrophoresis as final fractionation step. Preparations of troponin T with purities of more than 95 % were applied as antigen in the immunization of rabbits. Several standard immunological techniques were used to determine additions of turkey meat in food products heated to different temperatures (including roasting temperature). That way, it was possible to also detect additions of chicken meat on the basis of a partial identity between troponins T from turkey and chicken muscle.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
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