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  • 1
    Electronic Resource
    Electronic Resource
    Crossed Immunoelectrophoretic Analysis of Precipitins to Candida albicans in Deep Candida Infection (1975)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 4 (1975), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: By means of crossed immunoelectrophoresis with intermediate gel the precipitins to C. albicans in sera from 18 patients with deep Candida infection were compared with the findings in a control material consisting of sera from 169 'normal' controls (mainly healthy blood donors), 25 patients with oral candidiasis, 14 patients with vaginal candidiasis, and 6 persons without candidiasis giving false positive reactions by the double diffusion method. Up to 20 different precipitins were found in individual sera, and precipitins were demonstrated in all sera except in 6 'normal' controls. Of 692 precipitins 682 (98%) were identified and titrated in terms of the 78-component reference system described earlier for C. albicans. No free Candida antigens were found, but would have been detected by the method. The best criterion for discrimination between sera from patients and controls was the precipitin score, which accounts for number of precipitins and for titres. A precipitin score of more than 6 1/2 had a diagnostic sensitivity of 88.9% and a specificity of 99.5%. Eleven precipitin specificities were found solely in the patients' sera, but the highest sensitivity for a single precipitin was 33%. Until more evidence has accumulated in favour of a monospecific immunoassay it is therefore rational to use a complex antigen extract for further evaluations of diagnostic Candida serology and a short cut to standardization would be the introduction of the method used in the present study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1975.tb03830.x
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  • 2
    Electronic Resource
    Electronic Resource
    Specific long-lasting potentiation of synaptic transmission in hippocampal slices (1977)
    [s.l.] : Nature Publishing Group
    Nature 266 (1977), S. 736-737 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Figure 1 shows the location of a typical CA1 neurone with reference to the layers of the hippocampal slice, and the arrangement of the stimulating and recording electrodes used to study two independent pathways, one in the layer of basal dendrites (str. oriens) and the other in the layer of apical ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/266736a0
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  • 3
    Electronic Resource
    Electronic Resource
    Formation of bimodal crystal textures in polypropylene (1975)
    Springer
    Journal of materials science 10 (1975), S. 870-886 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Electron microscopy, selected area electron diffraction, X-ray diffraction, and dynamic-mechanical testing have been used to study flow-crystallized and hot drawn isotactic polypropylene. As a result of these investigations, it was found that bimodal crystal textures can apparently be formed by at least two different treatments, but the corresponding morphologies are completely different. Flow-induced crystallization was observed to result in a microstructure of lamellae oriented perpendicular to the flow direction, while hot drawing of polypropylene films above a critical temperature produced a morphology of microfibrils lying parallel to the draw direction. Below this critical temperature, drawing produced a fibrillar morphology having only a typical unimodal fibre texture. As a result of information obtained here, a mechanism involving epitaxial deposition of chain segments onto growing lamellae is concluded to be responsible for formation of the bimodal crystal texture in flow-crystallized material.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01163082
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  • 4
    Electronic Resource
    Electronic Resource
    Studies of mitochondria and mitochondrial DNA extracted from organelles harboring an intramitochondrial virus (1977)
    Springer
    Molecular and cellular biochemistry 14 (1977), S. 129-133 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary (1) Two viper cell lines were investigated, one which harbors IMV in the mitochondria (VSW cells) and one without detectable IMV (VH3 cells). (2) The size of closed circular mtDNA molecules from both VSW and VH3 cells was found to be significantly greater (5.4 to 5.6µ) than the contour lengths of typical mammalian cells (4.8 to 5.2µ). (3) A small percentage of mini-circles ranging in size from 0.1 to 0.6µ was observed to band with closed circular mtDNA from both cell lines. Minicircles were especially abundant in VH3 cells. (4) MtDNA from VSW cells contained 34.1% dimers plus oligomers (10.2% oligomers), whereas VH3 cells had only 14.8% dimeric and oligomeric forms (5.4% oligomers). (5) Treatment of VSW cells with 1µg/ml ethidium bromide for 48 hours resulted in an increased incidence of IMV (IMV in 15% of mitochondrial sections) as compared with untreated VSW cells (IMV in 3% of mitochondrial sections).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01734176
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  • 5
    Electronic Resource
    Electronic Resource
    Glycogen depletion pattern and lactate accumulation in leg muscles during recreational downhill skiing (1978)
    Springer
    European journal of applied physiology 38 (1978), S. 261-269 
    Details
    ISSN: 1439-6327
    Keywords: Muscle glycogen ; Muscle and blood lactic acid ; Fibre type glycogen depletion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Twenty-eight adult female and male subjects performed recreational skiing, which took place in terrain with short and long slopes. Muscle biopsies were obtained before and after the skiing from the lateral portion of the quadriceps femoris for histochemical evaluation of glycogen depletion in individual fibres and biochemical analysis of glycogen and lactate content. Blood lactate was also determined. After a day of skiing the decline in muscle glycogen averaged 33 mM/kg in both short and long slope terrain. Technically advanced skiers had a reduction of 38 mM/kg and less advanced skiers had a reduction of 28 mM/kg (p〈0.05). In most subjects there was a predominant loss of glycogen from the slow-twitch fibres (ST). In the most talented skiers glycogen depletion was also observed in the fast-twitch a fibres (FTa). In contrast a pronounced depletion of the fast-twitch b fibres (FTb) was observed in the technically unskilled skier. Muscle lactate concentrations ranged 2–8 mM/kg, the higher values observed in the technically better skiers. Blood lactate values reflected muscle lactate at a 1–2 mM/l lower level. The obtained results suggest that recreational downhill skiing performed by experienced skiers can be characterized as dynamic exercise and that glycogen depleted muscle fibres may contribute to the fatigue experienced after a day of skiing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423108
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  • 6
    Electronic Resource
    Electronic Resource
    Polypropylene fibers melt-spun in the presence of torsional shear (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 19 (1975), S. 1755-1766 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Melt-spun fibers of isotactic polypropylene have been formed using specific combinations of shearing and elongational flow. Such rheological conditions were achieved with a take-up device capable of subjecting molten polymer filaments continuously to various combinations of torsional and elongational strains. Relationships were determined between mechanical properties of the resulting fibers and their molecular organizations. The results obtained indicated that these flow fields would produce fibers whose mean-preferred-chain-orientation directions ranged between being tilted radially from a direction parallel with the fiber axis (no torsional shear strain imposed) and having some additional tilt that is tangential with respect to the fiber circumference (some torsional shear strain imposed). It is not possible to resolve the observed helical chain tilts into contributions from crystalline and amorphous regions, respectively.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1975.070190625
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  • 7
    Electronic Resource
    Electronic Resource
    Flow-induced crystallization of polypropylene in stretched ribbons (1976)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 16 (1976), S. 217-221 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Ribbons of molten polypropylene have been extruded into a thermostatted chamber and subjected to elongations ranging as high as 4400-fold. Some of these ribbons have been shown to possess the unusual ability of recovering nearly all of the strain from large levels of stretch (≤100 percent). This property, as well as the magnitude of the tensile yield drop, has been found to be directly proportional to the uniaxial character of the flow present at the moment of crystallization. The structure in the final solid films has been characterized by wide angle X-ray diffraction and crystal orientation distribution. Both of these factors have been found to be proportional to the character of flow at the moment of crystallization. Finally, the temperature in the thermostatted chamber has been shown to affect the character of the flow.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760160316
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  • 8
    Electronic Resource
    Electronic Resource
    Crystal nucleation in sheared polymer melts (1978)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 18 (1978), S. 215-221 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: A semiquantitative procedure has been developed for analyzing crystal nucleation in undercooled polymer melts that are undergoing flow. This analysis was applied to the specific ease of molten high density polyethylene experiencing low levels of shearing flow. A custom-made concentric cylinder viscometer, which could be operated by the Rheometrics mechanical spectrometer instrument, was used to make simultaneous measurements of transmitted torque and optical anisotropy in isothermal melts, The result of the analytical procedure developed here was molecular Size-dependence of chain distensions caused by prevailing shear. This distribution function was verified by testing against experimentally obtained values of birefringence. Total entropy reduction resulting from this distorted state was then calculated, and the corresponding increase in free energy was found to be at least enough to account for comparable crystal nucleation rates in flowing melts at higher temperatures and in quiescent melts at lower temperatures.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760180308
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