Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Celluläre Immunreaktionen gegenüber homologen leberspezifischen Antigenen (HLP) bei chronischen Leberentzündungen (1974)
    Springer
    Journal of molecular medicine 52 (1974), S. 246-248 
    Details
    ISSN: 1432-1440
    Keywords: Chronic active hepatitis ; Cellular immunity ; Liver specific antigens ; Migration inhibition test ; leukocyte ; Chronisch aktive Hepatitis ; Celluläre Immunität ; Leberspezifische Antigene ; Migrations-Inhibitions-Test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es wird eine Zweiphasentechnik des Leukocyten-Migrations-Inhibitionstestes beschrieben und zum Nachweis einer cellulären Immunreaktion gegenüber Leberantigenen bei chronisch-entzündlichen Lebererkrankungen eingesetzt. Mit dieser Technik wurde bei 85% der Patienten mit hypergammaglobulinämischer Verlaufsform einer chronisch-aktiven Hepatitis und 42% der Patienten mit aktiv fortschreitenden kryptogenen Lebercirrhosen eine Migrationshemmung nachgewiesen. Lebergesunde Kontrollen zeigten keine, Patienten mit dem Zustand nach Hepatitis und chronisch-persistierender Hepatitis selten eine Leukocytenmigrationsinhibition gegenüber leberspezifischen Antigenen. 17% der Patienten mit chronisch-aktiver Hepatitis, die länger als 6 Monate unter einer immunosuppressiven Therapie standen, ließen noch eine schwache Migrationsinhibition erkennen. Die Befunde legen nahe, daß celluläre Immunreaktionen gegenüber leberspezifischen Antigenen bei bestimmten Verlaufsformen einer chronisch-entzündlichen Lebererkrankung pathogenetisch von Bedeutung sind.
    Notes: Summary A two-phase technique of the leukocyte migration inhibition test is described. Cellular immunity to homologous liver specific proteins in patients with chronic inflammatory liver diseases has been assayed using this test system. Inhibition of migration was observed in 85% of the patients with chronic active hepatitis and in 42% of the patients with cryptogenic cirrhosis of the liver. Healthy subjects with no history of liver disease were used as controls and showed no inhibition of migration to the liver antigens. Patients with healed hepatitis and chronic persistent hepatitis rarely had abnormal migration indices. A statistically insignificant inhibition of migration was found only in patients with chronic active hepatitis treated with azathioprin and prednison. The results support the view of the importance of cell- mediated immune reactions towards liver antigens in the pathogenesis of chronic active hepatitis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01468598
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    HL-A bei Lebererkrankungen (1973)
    Springer
    Journal of molecular medicine 51 (1973), S. 1075-1076 
    Details
    ISSN: 1432-1440
    Keywords: HL-A ; liver diseases ; HL-A ; Leberkrankheiten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Patienten mit akuter Hepatitis (AH), chronisch persistierender Hepatitis (CPH), autoimmuner chronisch aggressiver Hepatitis (CAH), Lebercirrhose (CI), metabolisch bedingten Leberkrankheiten (MLK) die in der II. Medizinischen Universitätsklinik und Poliklinik behandelt oder kontrolliert werden, und normale Kontrollpersonen wurden nach der NIH-Standardmethode HL-A typisiert. Bei der chronisch aggressiven Hepatitis wurden HL-A 1 und 8 statistisch signifikant häufiger gefunden (p〈0,01).
    Notes: Summary Patients treated or controlled at the Second Medical University Clinic and Outpatientclinic Mainz for acute hepatitis (AH), chronic persistent hepatitis (CPH), autoimmune chronic aggressive hepatitis (CAH), liver cirrhosis (CI). or liver diseases due to metabolic disorders (MLK) as well as normal controls were typed for HL-A by the NIH standard test. HL-A 1 and 8 were found to be associated with autoimmune hepatitis (p〈0.01).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01468323
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Scleroedema adultorum und Plasmocytom (1974)
    Springer
    Archives of dermatological research 248 (1974), S. 379-385 
    Details
    ISSN: 1432-069X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary A 37-year-old man suffering of a scleredema adultorum (Buschke) developed peripheral neuropathy of the shoulder-arm area due to root compression by an extradural growing process localized premedullary at C 7/Th 1. After neurosurgical intervention the histologic examination revealed a multiple myeloma. The diagnosis was confirmed by paraproteinemia and paraproteinuria documented first by paper electrophoresis and then identified by immunoelectrophoretic demonstration of a gamma-G-gammopathia. A possible relationship of scleredema and multiple myeloma is discussed.
    Notes: Zusammenfassung Bei einem 37jährigen Patienten mit einem im Bereich beider Arme und Schultern lokalisiertenScleroedema adultorum (Buschke) traten fast gleichzeitig sensomotorische Ausfälle der Schulter-Arm-Region auf. Diese waren durch einen prämedullär, extradural in Höhe von C 7/Th 1 gelegenen raumfordernden Prozeß hervorgerufen, dessen histologische Untersuchung ein Plasmocytom ergab. Diese Diagnose wurde durch eine Paraproteinämie und eine Paraproteinurie bestätigt, die sich durch papierelektrophoretische und immunelektrophoretische Untersuchungen als Gamma-G-Gammopathie charakterisieren ließ. Die Frage bleibt offen, ob es sich hierbei um ein mehr als zufälliges Zusammentreffen beider Erkrankungen handelt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00595556
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Human T helper cells reactive with somatic bacterial antigens belong to the Th1 subset (1994)
    Springer
    Medical microbiology and immunology 183 (1994), S. 169-175 
    Details
    ISSN: 1432-1831
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The aim of this study was to characterize the cytokine secretion patterns of human T helper cells from healthy donors reactive with somatic antigens from various bacteria, the nematode Anisakis and tetanus toxoid. From the peripheral blood of four healthy donors we have established 70 T cell lines reactive with antigens from Yersinia, Salmonella, Morganella, Klebsiella, Serratia, Escherichia, Chlamydia, Shigella, Streptococcus, tetanus toxoid and Anisakis, respectively. Our results show that all T cells reactive with bacteria produce interferon-γ(IFN-γ) and tumor necrosis factor-α (TNF-α), but no interleukin (IL)-4 and no or very little IL-2 and IL-10 and, thus, belong to the Th1 subset, while T cells reactive with tetanus toxoid or Anisakis belong to the ThO subset with production of IFN-γ, IL-2, IL-4, IL-10 and TNF-α. In summary, our data further substantiate the concept of a functional diversity of human T helper cells with respect to their cytokine profiles. Furthermore, they indicate that a Th1 cytokine profile is not restricted to intracellular bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196051
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Hepatitis C virus associated primary hepatocellular carcinoma in a noncirrhotic liver (1993)
    Springer
    Journal of molecular medicine 71 (1993), S. 49-53 
    Details
    ISSN: 1432-1440
    Keywords: Hepatocellular carcinoma (HCC) ; Non-cirrhotic liver ; Hepatitis B virus (HBV) ; Hepatitis C virus (HCV) ; Nodular regenerative hyperplasia (NRH)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The case of a 71-year-old man with a primary hepatocellular carcinoma in a non-cirrhotic liver is reported. There were no risk factors of hepatocellular carcinoma (HCC)-like liver cirrhosis, alcohol drinking, tobacco smoking, exposure to vinyl chloride, thorotrast, aflatoxin or α1-antitrypsin deficiency. Serologically, the patient was positive for antibodies to the hepatitis B virus (anti-HBc, anti-HBs) and for anti-hepatitis C virus (HCV) antibodies. Virologically, positive and negative strands of HCV RNA could be detected in the patient's serum and tumorous liver tissue by reverse transcription polymerase chain reaction as a sign of persistent HCV replication. Histologically, the HCC was completely surrounded by liver tissue which showed the signs of nodular regenerative hyperplasia. Indeed, the mechanism of hepatocarcinogenesis remains to be clarified. However, this case supports the observation that HCC may also develop in patients with HCV infection without preexisting liver cirrhosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00210964
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Acute hepatic failure: limitations of medical treatment and indications for liver transplantation (1993)
    Springer
    Journal of molecular medicine 71 (1993), S. 875-881 
    Details
    ISSN: 1432-1440
    Keywords: Acute hepatic failure ; Liver transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00185596
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Sustained elimination of hepatitis B virus from serum induced in a patient with chronic hepatitis B and advanced human immunodeficiency virus infection (1994)
    Springer
    Journal of molecular medicine 72 (1994), S. 1030-1036 
    Details
    ISSN: 1432-1440
    Keywords: Human immunodeficiency virus ; Hepatitis B virus ; Interferon-α ; Corticosteroid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A 48-year-old male patient was admitted with acquired immunodeficiency syndrome (stage III, Centers for Disease Control 1993) and viremic hepatitis B. Blood CD4 count was 15/μl. Discontinuation of prednisolone, previously prescribed by the patient's family practitioner because of elevated liver enzymes, resulted in severe hepatitis (alanine aminotransferase 〉 300U/1). Administration of interferon-α, (9 × 106U s.c. 3 × weekly) was initiated. Serum markers of viral replication disappeared, and aminotransferase levels returned to normal within a few weeks. The patient's serum was found negative for HBsAg after 3 months. Immunohistochemical analysis of liver biopsies before and during interferon therapy showed disappearance of all hepatitis B virus antigens and a marked reduction in inflammatory activity. Hepatitis B virus seroconversion remained stable until the patient died from the syndrome 2 years later. This case shows that in spite of severe HIV-associated immune deficiency with CD4 counts constantly below 100/μl, interferon-α can lead to sustained serological and histological improvement of viremic hepatitis B. Previous administration and discontinuation of cortisone may have helped to reach this effect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00577750
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Correlation of anti-cytoskeleton antibody activities in synovial fluid with interleukin-6 in patients with osteoarthritis and inflammatory joint disease (1990)
    Springer
    Journal of molecular medicine 68 (1990), S. 685-691 
    Details
    ISSN: 1432-1440
    Keywords: Synovial fluid ; IL-6 ; Cytoskeleton ; Antibodies ; ELISA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Synovial fluids and sera from patients with rheumatoid arthritis, psoriatic arthritis, yersinia arthritis, Behçet's syndrome, Crohn's disease, and osteoarthritis were tested for antinuclear antibodies and antibodies to five cytoskeletal components in sensitive enzyme-linked immunosorbent assay (ELISA) systems and for IL-6 concentrations in a proliferation assay (IL-6 dependent hybridoma cell line B13.29, subclone B9). Statistically significant correlations between antibody activities and IL-6 levels were found for vimentin antibodies (r= 0.56; p〈0.05) and actin antibodies (r= 0.44;p〈0.05). In patients with chronic and active disease like rheumatoid arthritis and psoriatic arthritis, optical densities measured by vimentin- and actin-ELISA were significantly different from those measured in patients with osteoarthritis. To date only a few reports exist concerning the incidence of antibodies in synovial fluids. We have shown to our knowledge for the first time that IL-6 seems to induce synovial fluid antibody activities restricted to cytoskeletal components of synoviocytes (i.e., vimentin and actin). Synovial fluid antibody activities against vimentin and actin appear to be markers of activity in patients with inflammatory joint disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01667017
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Sinusoidal endothelial liver cells in vitro release endothelin — Augmentation by transforming growth factorβ and Kupffer cell-conditioned media (1991)
    Springer
    Journal of molecular medicine 69 (1991), S. 387-391 
    Details
    ISSN: 1432-1440
    Keywords: Endothelin ; Liver ; Sinusoidal endothelial cells ; Transforming growth factorβ
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Endothelin is the most potent vasoconstrictor peptide known today. Using a radioimmunoassay for endothelin, we measured immunoreactive endothelin in culture media of guinea pig sinusoidal endothelial liver cells and human umbilical vein endothelial cells. A time-dependent release of immunoreactive endothelin by confluent sinusoidal endothelial liver cells in culture was found. Sinusoidal endothelial liver cells produced similar amounts of immunoreactive endothelin as umbilical vein endothelial cells, about 900 pg/μg DNA per 24 h. In the presence of transforming growth factorβ a dose-dependent increase of immunoreactive endothelin release was measured. The maximal increase of 50% was found at a concentration of 1 ng transforming growth factor per ml. To a similar extent Kupffer cell-conditioned media augmented the release of immunoreactive endothelin by sinusoidal endothelial liver cells, especially when Kupffer cells had been stimulated by endotoxin. Endotoxin itself did not alter the release of immunoreactive endothelin. Endothelin released by sinusoidal endothelial liver cells might influence the pericytes of the liver, i.e., the Ito-cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01647411
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Zur Vitalitätsprüfung isolierter Rattenleberzellen (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 199-209 
    Details
    ISSN: 1433-8580
    Keywords: Rat liver cells ; Technique of isolation ; Viability tests ; Rattenleberzellen ; Isolierungstechnik ; Vitalitätstests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Rattenleberzellen wurden mittels extrakorporaler Leberperfusion unter Verwendung eines Hyaluronidase-Kollagenase-haltigen bzw. enzymfreien Perfusionsmediums isoliert. Die isolierten Leberzellen wurden auf ihr Verhalten gegenüber Trypanblau, FDA, in der Phasenkontrastbetrachtung und im Luciferin-Luciferase-Test untersucht. Darüber hinaus wurde der ATP-Gehalt der Zellen sowie die spontane Abgabe von51Cr aus markierten Zellen bestimmt. Mit der Trypanblau- und der FDA-Technik zeigen enzympräparierte Zellen für einige Stunden Vitalitätsmerkmale, nicht hingegen enzymfrei präparierte Zellen. Im Gegensatz dazu erfüllen in der Phasenkontrastbetrachtung und im Luciferin-Luciferase-Test enzymfrei präparierte Leberzellen Vitalitätskriterien länger und in einem höheren Prozentanteil als enzympräparierte Zellen. Der ATP-Gehalt der enzympräparierten Zellen liegt signifikant höher verglichen mit enzymfrei präparierten Zellen. Die spontane ATP-Freisetzung aus isolierten Rattenleberzellen beträgt bei 1 × 106 Zellen pro Milliliter weniger als 1 ng/ml.51Cr wird von markierten Rattenleberzellen innerhalb von 5 Std bis zu 50% der primär gebundenen Aktivität abgegeben. Die Ergebnisse gestatten eine Aussage darüber, welche dieser Methoden sich für eine quantitative Erfassung immunologisch bedingterin vitro-Schädigungen von Leberzellen eignet.
    Notes: Summary Rat liver cells have been isolated by extracorporal liver perfusion using an enzymefree or enzyme containing perfusion medium. The vitality of the liver cells has been studied by the trypan-blue-technique, by FDA, by phase contrast microscopy and by the luciferin-luciferase-test. Furthermore the ATP ratio of the liver cells and their spontaneous ATP — as well as spontaneous51Cr release have been measured. Enzyme prepared liver cells had vitality criteria only a few hours after the isolation when studied by the trypan-blue or FDA technique. Using these methods it was impossible to define the vitality of enzymefree prepared cells. In contrast to these results the phase contrast microscopy and the luciferin-luciferase-test demonstrated, that enzymefree prepared liver cells fullfill vitality criteria longer and in a higher percentage compared with enzyme prepared hepatocytes. The ATP ratio of enzyme prepared liver cells was significantly higher. The spontaneous ATP release of both cell preparations was 1 ng pro 1 × 106 cells/ml. The51Cr release of isolated liver cells was about 50% of the primary bound activity. The investigations show what kind of method may be suitable to measure quantitativelyin vitro damages of liver cells induced by immune reactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851667
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...