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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 38 (1979), S. 143-143 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0584
    Keywords: Leukämie ; Elektronenspin-Resonanz (ESR) ; Antioxidantien ; Leukemia ; ESR ; Antioxidants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary ESR investigations of lyophilized blood of patients with acute lymphatic leukemia exhibit an increase in spin concentration and an additional peak not present in control samples. This peak disappears almost completely concomittantly with a reduction in spin concentration after treating the patients with prednisolone, vincristine, and doxorubicin or after addition of 5 mM of CuCl2 to blood samples. The results show that the ESR spectra of lyophilized blood samples can be utilized to follow the effect of therapy. Moreover, they suggest that the leukemic blood possesses a high concentration of antioxidants.
    Notes: Zusammenfassung ESR-Untersuchungen an lyophilisiertem Blut von Patienten mit akuter lymphatischer Leukämie zeigten eine Zunahme in der Spinkonzentration. Außerdem trat ein zusätzliches Signal auf, welches in Kontrollproben nicht vorhanden ist. Wenn man die Patienten mit Prednisolen, Vincristin und Doxorubicin behandelt, verschwindet gleichzeitig mit einer Abnahme der Spinkonzentration dieses Signal fast vollständig. Ein ähnlicher Effekt wurde erhalten, wenn entsprechende Blutproben mit 5 mM CuCl2 versetzt wurden. Die Ergebnisse zeigen, daß die ESR-Spektren von lyophilisierten Blutproben als Indikator für den Therapie-Verlauf benutzt werden können. Diese Ergebnisse deuten darüber hinaus darauf hin, daß leukämisches Blut eine hohe Konzentration an Antioxidantien besitzt.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 334 (1961), S. 399-418 
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The presence of alkaline phosphatase was investigated in slide preparations and smears of lymph nodes with the azo dye method. In the case of normal or hyperplastic lymph nodes, capillaries and arterioles are regularly and most strongly positive; the phosphatase activity in the endothelium of the venules, the larger blood vessels and the lymph vessels is irregular. One occasionally finds a few positive fibroblasts in the capsule and trabeculae. In addition, the pulpa sometimes shows a reaction in the region of the sinus, especially of the marginal sinus, and in the region of the germinal centers which appears to be associated with fibers. The activity in the region of the germinal centers is most fully developed with severe follicular lymphatic hyperplasia. A considerable degree of alkaline phosphatase activity can be noted at the edge of tubercles and tubercular caseous foci and in the scarified areas of the lympho-granulomatosis. In lymph node smears, elements interpreted as large reticulum cells were found to be about 16% phosphatase active; in part these cells are for certain vascular endothelium and fibroblasts. In the smears, we also found small lymphoid reticulum cells which gave a clear cut phosphatase reaction. No alkaline phosphatase activity could be demonstrated in the tissue mast cells, at least, that is, in their granula. Phosphatase activity could be clearly demonstrated in slide preparations as in smears in a part of the cells from 2 lymphosarcomas.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Virchows Archiv 335 (1962), S. 491-512 
    ISSN: 1432-2307
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The esterase activity of cells of lymph nodes was studied in sections and touch preparations by using α-naphthyl-acetate and naphthol-AS-acetate as substrates. The reaction with α-naphthyl-acetate was always more intense than with naphthol-AS-acetate, except in tissue mast cells. We found the most positive reaction in the phagocytic cells; namely in the sinus endothelial cells, in the reticulum cells of the pulp and follicles (here especially in the cells that phagocytize nuclear debris), and in the epithelioid cells and Langhans giant cells. The plump epithelioid cells reacted much more intensely than the thinner ones. The tissue mast cells, the Hodgkins' cells, and the Sternberg giant cells show edless activity. The regenerating cells of the lymph nodes, the basophilic stem cells and germinoblasts, were esterase negative, as were the lymphocytes and plasma cells. By means of their positive reaction in touch preparations small reticulum cells could be differentiated from the lymphocytes. In addition, some of the middle reticular “Reizzellen” could be classified with the histiocytes. The esterase reaction provided a good method of surveying the number and distribution of functionally active reticulum cells. The cells of monocytic leukemia showed esterase activity enabling them to be differentiated readily from paramyeloblasts. The esterases as demonstrated probably belonged to the generalGomori type, and possibly were ester-splitting peptidases.
    Notes: Zusammenfassung Die Esteraseaktivität der Lymphknotenzellen wurde mit den Substraten α-Naphthylacetat und Naphthol-AS-Acetat im Schnitt- und Tupfpräparat geprüft. Die Reaktion mit α-Naphthylacetat ist stets stärker als diejenige mit Naphthol-AS-Acetat, außer bei den Gewebsmastzellen. Die stärkste positive Reaktion finden wir in den phagocytosefähigen Zellen, nämlich in Sinusretothelien, Reticulumzellen der Pulpa und Follikeln (hier besonders in Kerntrümmerphagen !), Epitheloidzellen und Langhansschen Riesenzellen. Die „saftigen“ Epitheloidzellen reagieren viel stärker als die „dürren“. Eine geringere Aktivität zeigen die Gewebsmastzellen, Hodgkinzellen und Sternbergschen Riesenzellen. Die regenerierenden Zellen des Lymphknotens — basophile Stammzellen und Germinoblasten — sind wie die Lymphocyten und Plasmazellen esterasenegativ. Im Tupfpräparat konnten kleine Reticulumzellen durch ihre positive Reaktion von Lymphocyten abgegrenzt werden. Auch gelang es, einen Teil der „mittleren retikulären Reizzellen“ den Histiocyten zuzuordnen. Für die Diagnostik gestattet die Esterasereaktion einen guten Überblick über Zahl und Verteilung funktionell-aktiver Reticulumzellen im Schnitt- und Tupfpräparat. Ein Teil der Retikulosarkome enthält esterasepositive Zellen. Auch die Zellen der Monocytenleukämie zeigen großenteils Esteraseaktivität, wodurch sie sich leicht von den Paramyeloblasten unterscheiden lassen. Alle übrigen Neubildungen des blutbildenden Gewebes reagieren negativ. Die gefundenen Esterasen zählen wahrscheinlich zum „allgemeinen Typ“Gomoris und sind möglicherweise als esterspaltende Peptidasen anzusprechen.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Czechoslovak journal of physics 29 (1979), S. 468-472 
    ISSN: 1572-9486
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Two effects are considered to influence the time dependence of the transformed volume in a binary alloy: a) the impingement effect (IE), takes into account the mutual impingement of the growing particles and b) the impoverishment effect (PE), which considers the decrease of driving forces and the increase of the diffusion paths in the approximation of an impoverishing matrix. Combining both effects growth equations are obtained analogous toJohnson-Mehl-Avrami only for one-dimensional growth.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 30 (1975), S. 287-289 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 32 (1976), S. 269-269 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 35 (1977), S. 481-483 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 36 (1978), S. 325-325 
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Annals of hematology 35 (1977), S. 179-186 
    ISSN: 1432-0584
    Keywords: L-Asparaginase ; extrakorporale Therapie ; ALL ; Nebenwirkungen ; L-asparaginase ; Extracorporeal therapy ; ALL ; Side effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary L-asparaginase therapy is often limited by allergy or toxicity and probably in some cases by antibody mediated inactivation of the enzyme. These problems can be avoided by extracorporeal application of l-asparaginase. As the enzyme is a stable tetramer with high molecular weight it cannot pass through the dialysis membrane in contrast to the amino acid l-asparagin which is destroyed. The resulting l-asparagin depletion of the plasma is sufficient for therapeutic success. Effective extracorporeal l-asparaginase therapy is demonstrated in two patients with ALL who were resistant to other chemotherapy and could not be treated intravenously because of allergy and toxicity.
    Notes: Zusammenfassung Allergie mit der Gefahr der Anaphylaxie, Toxizität und möglicherweise Inaktivierung durch Antikörper begrenzen den Einsatz von L-Asparaginase in der Leukämiebehandlung. Diese Probleme können durch die extrakorporale Anwendung umgangen werden. Sie ist möglich, weil das Enzym als stabiles Tetramer mit höherem Molekulargewicht Dialysemembranen nicht passiert, während die Aminosäure L-Asparagin aus dem Körper entfernt werden kann und dann abgebaut wird. Für den Therapieeffekt ist allein die L-Asparagindepletion notwendig. Am Besipiel von zwei Patienten mit ALL, die gegenüber anderer Chemotherapie résistent und wegen Allergie bzw. Toxizität parenteral nicht mit L-Asparaginase behandelt werden konnten, wird das Verfahren beschrieben.
    Type of Medium: Electronic Resource
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