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  • 1
    ISSN: 1434-601X
    Schlagwort(e): 25.75.+r
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Positively charged pions and protons from collisions of Ne+NaF and Au+Au at 1 GeV/nucleon incident energy were measured near midrapidity. The center-of-mass pion spectra deviate from a Maxwell-Boltzmann distribution. The slope of the high-energy part of the pion spectra varies significantly with the system mass and little with the size of the reaction zone. While the total pion yield rises linearly with the number of participant nucleons, the highenergy component increases more than linearly.
    Materialart: Digitale Medien
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Naturwissenschaften 52 (1965), S. 646-647 
    ISSN: 1432-1904
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Allgemeine Naturwissenschaft
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Naturwissenschaften 53 (1966), S. 200-201 
    ISSN: 1432-1904
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Allgemeine Naturwissenschaft
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Mathematische Annalen 73 (1913), S. 241-244 
    ISSN: 1432-1807
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Mathematische Annalen 74 (1913), S. 301-308 
    ISSN: 1432-1807
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 41 (1995), S. 1057-1069 
    ISSN: 1432-1432
    Schlagwort(e): Gene evolution ; Seed protein genes ; Legumin ; Vicilin ; Gene family ; Sequence homology ; Intron/exon structure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Legumin-like 11S and vicilin-like 7S globulins are the main storage proteins of most angiosperms and gymnosperms. The subunits of the hexameric legumin are synthesized as a precursor comprising a N-terminal acidic α- and a C-terminal basic β-chain. The trimeric vicilin molecule consists of subunits composed of two symmetrical N- and C-terminal structural domains. In a multiple alignment we have compared the N-terminal and C-terminal domains of 11 legumns and seven vicilins of several dicot, monocot, and gymnosperm species. The comparisons using all six possible pairwise combinations reveal that the N-terminal and C-terminal domains of both protein families are similar to each other. These results together with data on the distribution of variable and conserved regions, on the positions of susceptible sites for proteolytic attack, as well as on the published 7S protein tertiary structure suggest that both protein families share a common single-domain ancestor molecule and lead to the hypothesis that a triplication event has occurred during the evolution of a putative legumin/vicilin ancestor gene. Moreover, the comparison of the intron/exon pattern reveals that at least three out of five intron positions are precisely conserved between the genes of both protein families, further supporting the idea of a common evolutionary origin of recent legumin and vicilin encoding genes.
    Materialart: Digitale Medien
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  • 7
    ISSN: 1432-2048
    Schlagwort(e): Key words: Chitinase  ;  β-1 ; 3-Glucanase ; α-Manno‐sidase ; Nicotiana ; Protein secretion ; Suspension culture ; Vacuolar enzymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract. We have investigated the possibility that vacuolar proteins can be secreted into the medium of cultured cells of Nicotiana tabacum L. Time-course and balance-sheet experiments showed that a large fraction, up to ca. 19%, of vacuolar α-mannosidase (EC 3.2.1.24) and vacuolar class I chitinase (EC 3.2.1.14) in suspension cultures accumulated in the medium within one week after subculturing. This effect was most pronounced in media containing 2,4-dichlorophenoxyacetic acid (2,4-D). Under comparable conditions only a small fraction, 1.8–5.1% of the total protein and ca. 1% of malate dehydrogenase (EC 1.1.1.37), which is localized primarily in the mitochondria and cytoplasm, accumulated in the medium. Pulse-chase experiments showed that newly synthesized vacuolar class I isoforms of chitinase and β-1,3-glucanase (EC 3.2.1.39) were released into the medium. Post-translational processing, but not the release of these proteins, was delayed by the secretion inhibitor brefeldin A. Only forms of the proteins present in the vacuole, i.e. mature chitinase and pro-β-1,3-glucanase and mature β-1,3-glucanase, were chased into the medium of tobacco cell-suspension cultures. Our results provide strong evidence that vacuolar α-mannosidase, chitinase and β-1,3-glucanase can be secreted into the medium. They also suggest that secretion of chitinase and β-1,3-glucanase might be via a novel pathway in which the proteins pass through the vacuolar compartment.
    Materialart: Digitale Medien
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  • 8
    ISSN: 1432-2048
    Schlagwort(e): Key words: 2S Globulin ; Narbonin (immunolocalization) ; Seed ; Storage Protein ; Translation (in vitro) ; Vicia
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract. Narbonin is a 2S protein from the globulin fraction of narbon bean (Vicia narbonensis L.) cotyledons. Its amino acid composition and the pattern of its regulated accumulation in developing seeds led to the suggestion that narbonin could be a storage protein. Therefore, it was expected to be present in protein bodies of the storage tissue cells. Comparison of the cDNA-derived amino acid sequence with a directly determined partial N-terminal sequence revealed that the primary translation product of narbonin mRNA lacks a transient N-terminal signal peptide (V.H. Nong et al., 1995, Plant Mol Biol 28: 61–72). Narbonin polypeptides that had been synthesized in a cell-free translation system supplemented with dog pancreas microsomes were not protected against degradation by posttranslationally added proteases (protease protection assay). In accordance with the lack of a signal peptide this indicates that the polypeptide was not cotranslationally sequestered into the microsomes. The protein-body fraction that had been isolated from mature narbon bean cotyledons by a non-aqueous gradient centrifugation procedure was free of narbonin; this was found in the soluble cell fraction. In electron micrographs, narbonin could be localized in the cytoplasm using the immuno gold-labelling technique. Previously, it had already been shown that narbonin is too slowly degraded during narbon bean germination to act as a storage protein. From all these results it has to be concluded that narbonin is a cytoplasmic protein which does not belong to the storage proteins in the restricted sense. Other possible functions are discussed.
    Materialart: Digitale Medien
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  • 9
    ISSN: 1573-5028
    Schlagwort(e): biosynthesis ; gene structure ; narbonin ; recombinant protein ; 2S globulin ; seed storage protein ; Victa narbonensis L.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract cDNA and genomic clones encoding narbonin, a 2S globulin from the seed of narbon bean (Vicia narbonensis L.), were obtained using the polymerase chain reaction (PCR) and sequenced. The full-length cDNA as well as genomic clones contain a single open reading frame (ORF) of 873 bp that encodes a protein with 291 amino acids comprising the mature narbonin polypeptide (M r ca. 33 100) and an initiation methionine. The deduced amino acid sequence lacks a transient N-terminal signal peptide. The genomic clones do not contain any intron. No homology was found to nucleic acid and protein sequences so far registered in sequence data libraries. The biosynthesis of narbonin during embryogenesis is developmentally-regulated and its pattern of synthesis closely resembles that of typical seed storage globulins. However, during seed germination narbonin was degraded very slowly, indicating that it may have other function than storage protein. Southern analysis suggests the existence of a small narbonin gene family. Narbonin genes were also found in four different species of the genus Vicia as well as in other legumes such as Canavalia ensiformis and Glycine max. In Escherichia coli a recombinant narbonin was produced which yielded crystals like those prepared from narbonin purified from seeds.
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Genetic resources and crop evolution 15 (1967), S. 311-350 
    ISSN: 1573-5109
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Beschreibung / Inhaltsverzeichnis: Summary First proposals for economic use of micro-algae by mass culture were made 25 years ago (Harder and von Witsch). Considering the increasing interest in this question experiments, success, experience, and problems of mass culture of algae are being reviewed. Detailed laboratory investigations on growth and metabolism of unicellular green algae rendered the basis for producing algae by pilot plants being operated, as a rule, with open containers in the open air; closed systems were used but rarely. Nearly in all cases additional heating and artificial light were abandoned. One of the main obstacles on the way for successful technical production of algae is the inability of sufficiently adapting the cultivation methods to the different and changable climatic conditions. Systematically isolating and collecting algae strains from natural sites and investigating the influence of temperature and light upon their productivity are ways in getting drains which are well adapted to specific climatic conditions and promise economic success. More detailed description follows of pilot plants from Hungaria, Czechoslovakia and Poland, which have been built after 1960 representing examples for further development of production methods and for successful use of natural resources. The protein content of algal products is just as high as in high quality animal food and higher than in the most vegetable feed-stuffs. Experiments made hitherto in feeding algae to animals proved to be appropriate. Before algae may be fed, however, on a large scale, more extensive feeding experiments with domestic animals are necessary. On principle, economic use of micro-algae may be expected in the future.
    Kurzfassung: Краткое содержание 25 лет тому назад было впервые предложено использование массовых культур одноклеточных водорослей в хозяйственных целях (Хардер и фон Вич). Поскольку интерес к этому вопросу постоянно воэрастает — дается обзор проведенных опытов, достигнутых результатов и возникших проблем. Тщательные лабораторные исследования роста и обмена веществ одноклеточных зеленых водорослей полоQzили основу их продукции в полупроизводс твенных условиях. Здесь, в большинстве случаев, применялись открытые бассейны под открытым небом; закрытые системы применялись реже. Культуры велись почти всегда без добавочного огрева и освещения. Главным затруднением на пути к рентабельному пронзводству водорослей является то, что ещё не удалось приспособить такую культуру К различным и изменчивым климатическим условиям. Систематическое выделение и собирание штаммов водорослей с различных местообитаний и изучение зависимости их продуктивности от температурных и световых условий — это те пути, которые помогут найти форму производотва, хорошо приспособленную к определенным климатическим условиям и перспективную с хозяйственной точки зрения. Более подробно описываются „заводы“ для культуры водорослей в Венгрии, Чехословакии и Польше, построенные после 1960 года, а также примеры дальнейшего развития методов производства и удачного использования данных естественных условий. Содержание белка у продуктов, полученных из водорослей так же высоко, как у полноценных кормовых продуктов животного происхождения и выше, чем у большинства растительных кормов. Опыты использования водорослей в кормлении животных показали уже их принципиальную пригодность. Однако, прежде чем перейти к кормлению водорослями в широком масштабе, необходимо будет провести еще много опытов по кормлению домашних животных. Хозяйственное использование одноклеточных водорослей в будущем представляется сейчас вполне возможным.
    Notizen: Zusammenfassung Vor nunmehr 25 Jahren wurde der erste Vorschlag gemacht, Kleinalgen durch Massenkultur wirtschaftlich zu nutzen (Harder und von Witsch). Im Hinblick auf das ständig wachsende Interesse an dieser Frage wird eine Übersicht über Versuche, Erfolge, Erfahrungen und Probleme der Algenmassenkultur gegeben. Eingehende Laboratoriums-Untersuchungen über Wachstum und Stoffwechsel einzelliger Grünalgen lieferten die Grundlagen für die Produktion von Algen in halbindustriellen Anlagen. Sie wurden meist mit offenen Kulturbehältern und unter freiem Himmel betrieben; seltener wendete man geschlossene Systeme an. Größtenteils wurde auf zusätzliche künstliche Beheizung und Belichtung verzichtet. Eine Hauptschwierigkeit auf dem Wege zu einer wirtschaftlich erfolgreichen Algenproduktion besteht darin, daß die Kulturverfahren noch nicht genügend an unterschiedliche und wechselnde Klimabedingungen angepaßt werden können. Systematische Isolierung und Sammlung von Algenstämmen von verschiedenen natürlichen Standorten und die Untersuchung der Temperatur- und Lichtabhängigkeit ihrer Produktivität sind Wege, um Formen zu gewinnen, die bestimmten klimatischen Bedingungen gut angepaßt sind und wirtschaftlichen Erfolg versprechen. Es werden Kulturanlagen aus Ungarn, der Tschechoslowakei und Polen näher beschrieben, die in den Jahren nach 1960 erbaut wurden und Beispiele für die Weiterentwicklung der Produktionsverfahren und für eine gelungene Ausnutzung natürlicher Gegebenheiten sind. Der Eiweißgehalt von Algenprodukten ist ebenso hoch wie bei hochwertigen tierischen und höher als bei den meisten pflanzlichen Futtermitteln. Die bisherigen Versuche über eine Nutzung der Algen in der Tierernährung haben ihre grundsätzliche Eignung bewiesen. Ehe Algen in großem Umfang verfüttert werden können, sind jedoch umfangreichere Fütterungsversuche mit Haustieren erforderlich. Grundsätzlich kann in der Zukunft mit einer wirtschaftlichen Nutzung von Kleinalgen gerechnet werden.
    Materialart: Digitale Medien
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