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Digitale Medien

Expression of calcium channel subunits in the normal and diseased human myocardium (1996)

Hasse, H. ; Kresse, A. ; Hohaus, A. ; [weitere]

Springer

Journal of molecular medicine 74 (1996), S. 99-104

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ISSN: 1432-1440

Schlagwort(e): L-type calcium channel ; β Subunit isoforms ; Human heart ; Cardiac hypertrophy

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract We investigated the expression of α1 and β subunits of the L-type Ca2+ channel on the protein level in cardiac preparations from normal human heart ventricles and from the hypertrophied septum of patients with hypertrophic obstructive cardiomyopathy (HOCM). 1,4-Dihydropyridine (DHP) binding and immunorecognition by polyclonal antibodies directed against the C-terminal amino acid sequences of the β2 and β3 subunits were used for detection and quantification of α1, β2, and β3 subunits. Bmax of high-affinity DHP binding was 35±2 fmol/mg protein in HOCM and 20±2 fmol/mg protein in normal human hearts (P〈0.05). In rabbit hearts the anti-β2 subunit antibody immunoprecipitated 80% of the total amount of DHP-labeled Ca2+ channels present in the assay. Under identical experimental conditions 25% of labeled Ca2+ channels were recovered in the immunoprecipitates of both normal and HOCM ventricles. A similar partial immunoprecipitation was observed in pig hearts. Immunoblot analysis demonstrated that the β2 subunit was associated with the DHP receptor/Ca2+ channel in cardiac muscle of rabbit, pig, and human heart. In neither of these purified cardiac Ca2+ channels was the β3 subunit isoform detected. Our results suggest that both α1 and β2 subunit expression is upregulated in HOCM in a coordinate manner.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00196785

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Digitale Medien

Secondary ion mass spectrometry round-robin study of relative sensitivity factors in gallium arsenide (1998)

Homma, Y. ; Tohjou, F. ; Masamoto, A. ; [weitere]

Chichester [u.a.] : Wiley-Blackwell

Surface and Interface Analysis 26 (1998), S. 144-154

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ISSN: 0142-2421

Schlagwort(e): SIMS ; GaAs ; round-robin ; sensitivity factor ; Chemistry ; Polymer and Materials Science

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Physik

Notizen: Round-robin studies on relative sensitivity factors (RSFs) in secondary ion mass spectrometry (SIMS) were conducted using bulk GaAs samples uniformly doped with various impurity elements. A total of 31 laboratories participated in two round-robins. More than 30 sets of relative ion intensities were obtained for B, Si, Cr, Mn, Fe, Cu, Zn, In and Te in GaAs. The RSFs for both positive and negative ions were derived for several types of SIMS instruments. The effect of primary ion incident angle was examined using quadrupole-based instruments and found to be the determining factor of the instrumental dependence of RSF. © 1998 John Wiley & Sons, Ltd.

Zusätzliches Material: 6 Ill.

Materialart: Digitale Medien

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