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  • 1
    Electronic Resource
    Electronic Resource
    Alterations in apolipoprotein B-48 in the postprandial state in NIDDM (1994)
    Springer
    Diabetologia 37 (1994), S. 1259-1264 
    Details
    ISSN: 1432-0428
    Keywords: Apolipoprotein B-48 ; triglyceride-rich lipoproteins ; NIDDM ; cholesterol ; triglyceride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The intestine is a major site of cholesterol synthesis and produces apolipoprotein B-48, which is critical for intestinal cholesterol absorption and secretion. The purpose of this study was to examine postprandial changes in apolipoprotein B-48 in diabetes. Six non-insulin-dependent diabetic patients and six non-diabetic control subjects were given a high-fat meal (1300 kcal) and blood samples were taken pre- and postprandially, from which the triglyceride-rich lipoprotein fraction was isolated by ultracentrifugation (density〈1.006 g/ml). Apolipoprotein B-48 was separated on 4–15% gradient gels and quantified as a percentage of the fasting concentration by densitometric scanning. Total protein, triglyceride and cholesterol in the triglyceride-rich lipoprotein fraction, blood glucose, and serum insulin were also measured. Diabetic patients exhibited a postprandial triglyceride-rich apolipoprotein B-48 profile significantly different from that of control subjects (p〈0.05). The triglyceride and total protein concentration in the triglyceride-rich lipoprotein fraction mirrored the post-prandial profile and apolipoprotein B-48 in both groups. Significantly different patterns for triglyceride (p〈0.02) and total protein (p〈0.05) following the fat-rich meal were observed in the two groups. Fasting and postprandial triglyceride-rich lipoprotein cholesterol and total apolipoprotein B were significantly higher in diabetic patients than in control subjects (p〈0.05). Since apolipoprotein B-48 is the structural protein of intestinally-derived lipoprotein particles, these studies suggest an abnormality in intestinal lipoprotein metabolism in diabetes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00399800
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  • 2
    Electronic Resource
    Electronic Resource
    A case of juvenile lipidosis: Electron microscopic, histochemical and biochemical studies (1968)
    Springer
    Acta neuropathologica 11 (1968), S. 122-139 
    Details
    ISSN: 1432-0533
    Keywords: Juvenile Lipidosis ; Electron Microscopy ; Histochemistry ; Biochemistry ; Granular Lipid Inclusions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Elektronenmikroskopische, histochemische und biochemische Untersuchungen eines Falles von juveniler Lipidose werden mitgeteilt. Die neuronalen Einschlüsse sind zumeist von einer unit membrane umgeben und zeigen fingerabdruckähnliche Muster. Membranovesiculäre Körperchen finden sich ebenfalls in den Nervenzellperikaryen; typische granuläre Lipofuscinkörperchen werden aber nicht gesehen. Die Astrocyten der Rinde enthalten eine ähnliche Art von Einschlüssen, von denen aber die Einschlüsse in den Astrocyten der Marksubstanz völlig verschieden sind. Die Lokalisation der Aktivität von saurer Phosphatase in allen Einschlußtypen läßt an ihre mögliche lysosomale Natur denken. Die biochemische Analyse deckt keine spezifischen Abweichungen auf.
    Notes: Summary Electron microscopic, histochemical and biochemical studies of a case of juvenile lipidosis have been presented. The neuronal inclusions were mostly unit membranebound with fingerprint-like patterns. Membrano-vesicular bodies were also found in the neuronal perikarya, but “typical” granular lipofuscin bodies were not seen. Cortical astrocytes contained a similar type of inclusion but the inclusions in the astrocytes in the white matter were quite different. The localization of acid phosphatase activity in all types of inclusions suggests their possible lysosomal nature. Biochemical analysis failed to pin-point any specific abnormalities.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690216
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  • 3
    Electronic Resource
    Electronic Resource
    Abnormal sarcolemmal nuclei encountered in several cases of dystrophia myotonica (1969)
    Springer
    Acta neuropathologica 12 (1969), S. 183-188 
    Details
    ISSN: 1432-0533
    Keywords: Dystrophia myotonica ; Electron Microscopy ; Sarcolemmal Nuclei ; Myofibrillar Material
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung In fünf Fällen von Dystrophia myotonica zeigte die elektronenoptische Untersuchung von bioptisch entnommenem Material zentrale und subsarkolemmale Kerne, die komplexe vacuoläre Areale um myofibrilläres Material enthalten. Diese bisher nicht beobachtete Veränderung könnte durch Verlagerung von myofibrillärem Material in den Kern während seiner Wanderung in das Faserzentrum durch Invagination, aber nicht Durchbrechung der Kernmembran bedingt sein.
    Notes: Summary In five cases of dystrophia myotonica electron microscope preparations of muscle taken at biopsy showed both centrally placed and subsarcolemmal nuclei containing complex vacuolar areas enclosing myofibrillar material. It is suggested that this appearence, which has not been seen in any other condition, may be due to the forcing of myofibrillar material into the nucleus during its migration to the centre of the fibre invaginating but not breaching its membrane.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00692505
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  • 4
    Electronic Resource
    Electronic Resource
    Elevated triglyceride-rich lipoproteins in diabetes (1996)
    Springer
    Acta diabetologica 33 (1996), S. 205-210 
    Details
    ISSN: 1432-5233
    Keywords: Apolipoprotein B-48 ; Apolipoprotein B-100 ; Triglyceride-rich lipoprotein ; Non-insulin-dependent diabetes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The role of the intestine in cholesterol metabolism in human diabetes in unclear, although abnormalities have been demonstrated in cholesterol synthesis and absorption in diabetic animals. This study examines the relationship between fasting and post-prandial apolipoprotein B-48 in type 2 (non-insulin-dependent) diabetic and non-diabetic subjects. Eight type 2 diabetic patients and ten healthy non-diabetic control subjects were given a high-fat meal (1300 kcal), and the triglyceride-rich lipoprotein fraction was isolated by ultracentrifugation (d〈1.006 g/ml) from fasting and post-prandial plasma. Apolipoprotein B-48 and apo B-100 were separated on 4%–15% gradient gels and quantified by densitometric scanning with reference to a purified low-density lipoprotein (LDL) apo B-100 preparation. Diabetic patients had significantly higher concentrations of apo B-48 and apo B-100 in both the fasting (P〈0.05) and post-prandial (P〈0.001) triglyceride-rich lipoprotein samples compared with non-diabetic subjects. The diabetic patients also exhibited a significantly different post-prandial profile for apo B-48 and apo B-100, with a prolonged increase and a later post-prandial peak, than the non-diabetic subjects (P〈0.01). These results suggest that the raised fasting triglyceride-rich lipoproteins, often found in diabetes, are associated with apo B-48 and may be derived from increased intestinal chylomicron production. The post-prandial pattern suggests an abnormality in intestinal production as well as hepatic clearance of apo B-48 in type 2 diabetes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02048544
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  • 5
    Electronic Resource
    Electronic Resource
    Intestinally derived lipoprotein particles in non-insulin-dependent diabetic patients with and without hypertriglyceridaemia (1995)
    Springer
    Acta diabetologica 32 (1995), S. 244-250 
    Details
    ISSN: 1432-5233
    Keywords: Apolipoprotein B-48 ; Triglyceride-rich lipoproteins ; Chylomicrons ; Hypertriglyceridaemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have previously demonstrated alterations in apolipoprotein B-48 metabolism in the post-prandial state in patients with non-insulin-dependent diabetes mellitus. This study investigates the relationship between hypertriglyceridaemia and post-prandial lipoprotein metabolism. Four groups of patients were examined: non-insulin-dependent diabetic patients, with normal serum triglyceride levels (serum triglyceride 〈2.1 mmol l−1; haemoglobin HbA1c 5.5%±0.4%); poorly controlled, non-insulin-dependent diabetic patients with hypertriglyceridaemia (serum triglyceride 〉2.1 mmol 1−1; HbA1c 8.8%±0.9%); nondiabetic subjects with serum triglycerides 〈2.1 mmoll−1; and non-diabetic subjects with hypertriglyceridaemia (serum triglyceride〉2.1 mmol l−1). Subjects were studied fasting and following a high-fat meal (1300 kcal). The triglyceride-rich lipoprotein fraction was isolated by ultracentrifugation (d〈1.006 g ml−1). Apoprotein B-48, apoprotein B-100 and apoprotein E were separated on 4%–15% gradient gels and quantified as a percentage of the fasting concentration by densitometric scanning. Triglyceride-rich lipoprotein apolipoprotein B-48 and apolipoprotein B-100 post-prandial profiles demonstrated a maximum increase either at 2 h or rising still further to a peak at 6 h before falling in the diabetic groups and hypertriglyceridaemic non-diabetic subjects when compared with the normotriglyceridaemic control subjects whose levels decreased after 2 h (P〈0.05). A significantly different triglyceride-rich lipoprotein apolipoprotein E profile was also exhibited by the diabetic patients (P〈0.05). Levels of triglyceride-rich lipoprotein, cholesterol, triglyceride, total protein and apoprotein B were elevated in the hypertriglyceridaemic subjects, both diabetic and non-diabetic. These results indicate that hypertriglyceridaemia is associated with altered metabolism and composition of post-prandial triglyceride-rich lipoprotein particles in both poorly controlled diabetic and non-diabetic subjects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00576257
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    Paper (German National Licenses)
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